Directed, strong, and reversible immobilization of proteins tagged with a β-trefoil lectin domain: A simple method to immobilize biomolecules on plain agarose matrixes

Bioconjugate Chemistry

Volumn 23, Issue 3, 2012, Pages 565-573

  López Gallego, Fernando ^a   Acebrón, Ivan ^b   Mancheño, Jose Miguel ^b   Raja, Sebastian ^b   Lillo, M Pilar ^b   Guisán Seijas, Jose Manuel ^a  

^a INSTITUTO DE CATÁLISIS Y PETROLEOQUÍMICA   (Spain)

^b INSTITUTO DE QUÍMICA FÍSICA ROCASOLANO   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING ENERGY; ENZYME IMMOBILIZATION; ORGANIC SOLVENTS; SCAFFOLDS (BIOLOGY);

AGAROSE; DIRECTED IMMOBILIZATION; ENHANCED GREEN FLUORESCENT PROTEIN; FUSION PROTEINS; HIGHLY STABLES; IMMOBILISATION; MATRIX; PORE FORMING TOXINS; REVERSIBLE IMMOBILIZATION; SIMPLE METHOD;

BINDING SITES;

ACETONITRILE; AGAROSE; ENHANCED GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; LECTIN; MYCOTOXIN; TREFOIL PEPTIDE; TRIACYLGLYCEROL LIPASE;

ARTICLE; ENZYME BINDING; ENZYME IMMOBILIZATION; ENZYME SUBSTRATE; GEOBACILLUS; GEOBACILLUS THERMOCATENOLATUS; HIGH TEMPERATURE; LAETIPORUS SULPHUREUS; MUSHROOM; NONHUMAN; PROTEIN DOMAIN; PROTEIN STRUCTURE; TEMPERATURE DEPENDENCE; TOXIN ANALYSIS;

BASIDIOMYCOTA; GEOBACILLUS; LAETIPORUS SULPHUREUS; LOTUS;

EID: 84858732424     PISSN: 10431802     EISSN: 15204812     Source Type: Journal    
DOI: 10.1021/bc2006237     Document Type: Article

References (48)

1. Sheldon, R. A. (2007) Enzyme immobilization: the quest for optimum performance Adv. Synth. Catal. 349, 1289-1307
2. Mateo, C., Palomo, J. M., Fernandez-Lorente, G., Guisan, J. M., and Fernandez-Lafuente, R. (2007) Improvement of enzyme activity, stability and selectivity via immobilization techniques Enzyme Microb. Technol. 40, 1451-1463 (Pubitemid 46482654)
3. Berne, C., Betancor, L., Luckarift, H. R., and Spain, J. C. (2006) Application of a microfluidic reactor for screening cancer prodrug activation using silica-immobilized nitrobenzene nitroreductase Biomacromolecules 7, 2631-2636 (Pubitemid 44530503)
4. Betancor, L., Berne, C., Luckarift, H. R., and Spain, J. C. (2006) Coimmobilization of a redox enzyme and a cofactor regeneration system Chem. Commun. (Cambridge) 3640-3642 (Pubitemid 44267653)
5. Wang, F., Guo, C., Liu, H. Z., and Liu, C. Z. (2007) Reversible immobilization of glucoamylase by metal affinity adsorption on magnetic chelator particles J. Mol. Catal. B: Enzym. 48, 1-7 (Pubitemid 47126964)
6. Mateo, C., Abian, O., Fernandez-Lafuente, R., and Guisan, J. M. (2000) Reversible enzyme immobilization via a very strong and nondistorting ionic adsorption on support-polyethylenimine composites Biotechnol. Bioeng. 68, 98-105 (Pubitemid 30163498)
7. Wang, P. Y., Tsai, S. W., and Chen, T. L. (2008) Improvement of enantioselectivity and stability of Klebsiella oxytoca hydrolase immobilized on Eupergit C 250L J. Chem. Technol. Biotechnol. 83, 1518-1525
8. Wang, L., Ran, Q., Tian, Y., Xu, J., Xian, Y., Peng, R., and Jin, L. (2010) Covalent immobilization of redox protein via click chemistry and carbodiimide reaction: Direct electron transfer and biocatalysis J. Colloid Interface Sci. 350, 544-550
9. Mateo, C., Grazú, V., Pessela, B. C. C., Montes, T., Palomo, J. M., Torres, R., López-Gallego, F., Fernández-Lafuente, R., and Guisán, J. M. (2007) Advances in the design of new epoxy supports for enzyme immobilization-stabilization Biochem. Soc. Trans. 35, 1593-1601
10. Guisán, J. M. (1988) Aldehyde-agarose gels as activated supports for immobilization-stabilization of enzymes Enzyme Microb. Technol. 10, 375-382
11. Puertas, S., Batalla, P., Moros, M., Polo, E., Del Pino, P., Guisan, J. M., Grazu, V., and de la Fuente, J. M. (2011) Taking advantage of unspecific interactions to produce highly active magnetic nanoparticle-antibody conjugates ACS Nano 5, 4521-4528
12. Godoy, C. A., de las Rivas, B., Grazu, V., Montes, T., Guisán, J. M., and López-Gallego, F. (2011) Glyoxyl-disulfide agarose: a tailor-made support for site-directed rigidification of proteins Biomacromolecules 12, 1800-1809
13. Mansfeld, J. and Ulbrich-Hofmann, R. (2000) Site-specific and random immobilization of thermolysin-like proteases reflected in the thermal inactivation kinetics Biotechnol. Appl. Biochem. 32, 189-195 (Pubitemid 32053171)
14. Cuetos, A., Valenzuela, M. L., Lavandera, I., Gotor, V., and Carriedo, G. A. (2010) Polyphosphazenes as tunable and recyclable supports to immobilize alcohol dehydrogenases and lipases: Synthesis, catalytic activity, and recycling efficiency Biomacromolecules 11, 1291-1297
15. Fuentes, M., Pessela, B. C. C., Maquiese, J. V., Ortiz, C., Segura, R. L., Palomo, J. M., Abian, O., Torres, R., Mateo, C., Fernández-Lafuente, R., and Guisán, J. M. (2004) Reversible and strong immobilization of proteins by ionic exchange on supports coated with sulfate-dextran Biotechnol. Prog. 20, 1134-1139 (Pubitemid 39059626)
16. Mateo, C., Bolivar, J. M., Godoy, C. A., Rocha-Martin, J., Pessela, B. C., Curiel, J. A., Muñoz, R., Guisan, J. M., and Fernandez-Lorente, G. (2010) Improvement of enzyme properties with a two-step immobilizaton process on novel heterofunctional supports Biomacromolecules 11, 3112-3117
17. Hedhammar, M., Gräslund, T., and Hober, S. (2005) Protein engineering strategies for selective protein purification Chem. Eng. Technol. 28, 1315-1325 (Pubitemid 41694144)
18. Saleemuddin, M. (1999) Bioaffinity based immobilization of enzymes Adv. Biochem. Eng. Biotechnol. 64, 203-226
19. Terpe, K. (2003) Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems Appl. Microbiol. Biotechnol. 60, 523-533 (Pubitemid 36169526)
20. Ueda, E. K. M., Gout, P. W., and Morganti, L. (2003) Current and prospective applications of metal ion-protein binding J. Chromatogr., A. 988, 1-23 (Pubitemid 36165010)
21. Luke, J. M., Carnes, A. E., Sun, P., Hodgson, C. P., Waugh, D. S., and Williams, J. A. (2011) Thermostable tag (TST) protein expression system: Engineering thermotolerant recombinant proteins and vaccines J. Biotechnol. 151, 242-250
22. Esposito, D. and Chatterjee, D. K. (2006) Enhancement of soluble protein expression through the use of fusion tags Curr. Opin. Biotechnol. 17, 353-358 (Pubitemid 44163452)
23. Hu, S. and Wong, D. T. (2009) Lectin microarray Proteom. Clin. Appl. 3, 148-154
24. Tetala, K. K. R. and Van Beek, T. A. (2010) Bioaffinity chromatography on monolithic supports J. Sep. Sci. 33, 422-438
25. Mao, X., Luo, Y., Dai, Z., Wang, K., Du, Y., and Lin, B. (2004) Integrated lectin affinity microfluidic chip for glycoform separation Anal. Chem. 76, 6941-6947 (Pubitemid 39600868)
26. Tang, J., Liu, Y., Yin, P., Yao, G., Yan, G., Deng, C., and Zhang, X. (2010) Concanavalin A-immobilized magnetic nanoparticles for selective enrichment of glycoproteins and application to glycoproteomics in hepatocelluar carcinoma cell line Proteomics 10, 2000-2014
27. Mancheño, J. M., Tateno, H., Goldstein, I. J., Martinez-Ripoll, M., and Hermoso, J. A. (2005) Structural analysis of the Laetiporus sulphureus hemolytic pore-forming lectin in complex with sugars J. Biol. Chem. 280, 17251-17259 (Pubitemid 41389193)
28. Mancheño, J. M. and Angulo, I. (2009) Fusion Proteins Having a Beta-Trefoil-Type Domain: Method for Obtaining Same and Use Thereof, WO121994.
29. Angulo, I., Acebron, I., de Las Rivas, B., Munoz, R., Rodriguez-Crespo, I., Menendez, M., Garcia, P., Tateno, H., Goldstein, I. J., Perez-Agote, B., and Mancheno, J. M. (2011) High-resolution structural insights on the sugar-recognition and fusion tag properties of a versatile b-trefoil lectin domain from the mushroom Laetiporus sulphureus Glycobiology 21, 1349-1361
30. Wiesbauer, J., Bolivar, J. M., Mueller, M., Schiller, M., and Nidetzky, B. (2011) Oriented immobilization of enzymes made fit for applied biocatalysis: non-covalent attachment to anionic supports using Zbasic2Module ChemCatChem 3, 1299-1303
31. Johnson, C. P., Jensen, I. E., Prakasam, A., Vijayendran, R., and Leckband, D. (2003) Engineered protein a for the orientational control of immobilized proteins Bioconjugate Chem. 14, 974-978 (Pubitemid 37152957)
32. Nordon, R. E., Craig, S. J., and Foong, F. C. (2009) Molecular engineering of the cellulosome complex for affinity and bioenergy applications Biotechnol. Lett. 31, 465-476
33. Cassimjee, K. E., Kourist, R., Lindberg, D., Wittrup Larsen, M., Thanh, N. H., Widersten, M., Bornscheuer, U. T., and Berglund, P. (2011) One-step enzyme extraction and immobilization for biocatalysis applications Biotechnol. J. 6, 463-469
34. Holland-Nell, K. and Beck-Sickinger, A. G. (2007) Specifically immobilised aldo/keto reductase AKR1A1 shows a dramatic increase in activity relative to the randomly immobilised enzyme ChemBioChem 8, 1071-1076 (Pubitemid 47183624)
35. Kim, M. I., Kwon, S. J., and Dordick, J. S. (2009) In vitro precursor-directed synthesis of polyketide analogues with coenzyme a regeneration for the development of antiangiogenic agents Org. Lett. 11, 3806-3809
36. Tielker, D., Rosenau, F., Bartels, K. M., Rosenbaum, T., and Jaeger, K. E. (2006) Lectin-based affinity tag for one-step protein purification Biotechniques 41, 327-332 (Pubitemid 46534896)
37. Godoy, C. A., de las Rivas, B., Bezbradica, D., Bolivar, J. M., López-Gallego, F., Fernandez-Lorente, G., and Guisan, J. M. (2011) Reactivation of a thermostable lipase by solid phase unfolding/refolding. Effect of cysteine residues on refolding efficiency Enzyme Microb. Technol. 49, 388-394
38. Geiser, M., Cebe, R., Drewello, D., and Schmitz, R. (2001) Integration of PCR fragments at any specific site within cloning vectors without the use of restriction enzymes and DNA ligase Biotechniques 31 (88-90) 92
39. Fernandez-Lorente, G., Godoy, C. A., Mendes, A., Lopez-Gallego, F., Grazu, V., de las Rivas, B., Palomo, J., Hermoso, J., Fernandez-Lafuente, R., and Guisan, J. (2008) Solid-phase chemical amination of a lipase from bacillus thermocatenulatus to improve its stabilization via covalent immobilization on highly activated glyoxyl-agarose Biomacromolecules 9 (9) 2553-2561
40. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding Anal. Biochem. 72, 248-254
41. Uskova, M. A., Borst, J. W., Hink, M. A., van Hoek, A., Schots, A., Klyachko, N. L., and Visser, A. J. (2000) Fluorescence dynamics of green fluorescent protein in AOT reversed micelles Biophys. Chem. 87, 73-84
42. Ladero, M., Santos, A., and Garca-Ochoa, F. (2001) Diffusion and chemical reaction rates with nonuniform enzyme distribution: An experimental approach Biotechnol. Bioeng. 72, 458-467
43. Borchert, A. and Buchholz, K. (1984) Improved biocatalyst effectiveness by controlled immobilization of enzymes Biotechnol. Bioeng. 26, 727-736 (Pubitemid 14608186)
44. Bolivar, J. M., Hidalgo, A., Sanchez-Ruiloba, L., Berenguer, J., Guisan, J. M., and Lopez-Gallego, F. (2011) Modulation of the distribution of small proteins within porous matrixes by smart-control of the immobilization rate J. Biotechnol. 155, 412-420
45. Jokerst, J. V., Chou, J., Camp, J. P., Wong, J., Lennart, A., Pollard, A. A., Floriano, P. N., Christodoulides, N., Simmons, G. W., Zhou, Y., Ali, M. F., and McDevitt, J. T. (2011) Location of biomarkers and reagents within agarose beads of a programmable bio-nano-chip Small 7, 613-624
46. Logan, T. C., Clark, D. S., Stachowiak, T. B., Svec, F., and Fréchet, J. M. J. (2007) Photopatterning enzymes on polymer monoliths in microfluidic devices for steady-state kinetic analysis and spatially separated multi-enzyme reactions Anal. Chem. 79, 6592-6598 (Pubitemid 47395043)
47. Teeuwen, R. L. M., Zuilhof, H., De Wolf, F. A., and Van Hest, J. C. M. (2009) Temperature-controlled positioning of fusion proteins in microreactors Soft Matter. 5, 2261-2268
48. Medin, A. (1995) Studies on Structures and Properties of Agarose, Uppsala University, Uppsala, Sweden.