Reactivation of a thermostable lipase by solid phase unfolding/refolding. Effect of cysteine residues on refolding efficiency

Enzyme and Microbial Technology

Volumn 49, Issue 4, 2011, Pages 388-394

  Godoy, César A ^a   de las Rivas, Blanca ^c   Bezbradica, Dejan ^b   Bolivar, Juan M ^a,b,c   López Gallego, Fernando ^a,b,c   Fernandez Lorente, Gloria ^c   Guisan, Jose M ^a  

^a CSIC   (Spain)

^b UNIVERSITY OF BELGRADE   (Serbia)

^c INSTITUTO DE FERMENTACIONES INDUSTRIALES CSIC   (Spain)

Author keywords

Additives; Cysteine oxidation; Enzyme reactivation; Immobilization; Lipase; Refolding

Indexed keywords

ACTIVITY RECOVERY; AGAROSE; AMINO GROUP; AQUEOUS MEDIA; BUFFER COMPOSITION; COVALENT ATTACHMENT; CYANOGEN BROMIDE; CYS RESIDUES; CYSTEINE OXIDATION; CYSTEINE RESIDUES; DIFFERENT EFFECTS; DITHIOTHREITOL; ENZYME REACTIVATION; EXPERIMENTAL CONDITIONS; GLYOXYL; GLYOXYL AGAROSE; HIGH CONCENTRATION; INITIAL ACTIVITY; MATRIX; MULTIPOINT; PROTEIN REFOLDING; REFOLDING; REFOLDING EFFICIENCY; SOLID DERIVATIVES; SOLID-PHASE;

AMINO ACIDS; BROMINE COMPOUNDS; EFFICIENCY; OXIDATION; PH EFFECTS;

ENZYME IMMOBILIZATION;

AGAROSE; CYANOGEN BROMIDE; CYSTEINE; DITHIOTHREITOL; GLYOXYLIC ACID; IMMOBILIZED ENZYME; TRIACYLGLYCEROL LIPASE;

ARTICLE; ENZYME IMMOBILIZATION; GEOBACILLUS; GEOBACILLUS THERMOCATENULATUS; NONHUMAN; OXIDATION; PH; PROTEIN FOLDING;

BIOTECHNOLOGY; CYSTEINE; DITHIOTHREITOL; ENZYME ACTIVATION; ENZYME STABILITY; ENZYMES, IMMOBILIZED; GEOBACILLUS; GLYOXYLATES; LIPASE; MODELS, MOLECULAR; OXIDATION-REDUCTION; PROTEIN REFOLDING; SEPHAROSE;

GEOBACILLUS THERMOCATENULATUS;

EID: 80051794269     PISSN: 01410229     EISSN: 18790909     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2011.06.018     Document Type: Article

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