Modulation of the distribution of small proteins within porous matrixes by smart-control of the immobilization rate

Journal of Biotechnology

Volumn 155, Issue 4, 2011, Pages 412-420

  Bolivar, Juan M ^a   Hidalgo, Aurelio ^b   Sánchez Ruiloba, Lucia ^c   Berenguer, José ^b   Guisán, José M ^a   López Gallego, Fernando ^a  

^a INSTITUTO DE CATÁLISIS Y PETROLEOQUÍMICA   (Spain)

^b CENTRO DE BIOLOGÍA MOLECULAR SEVERO OCHOA   (Spain)

^c INSTITUTO DE INVESTIGACIONES BIOMÉDICAS ALBERTO SOLS   (Spain)

Author keywords

Confocal laser scanning microscopy; Fluorescent proteins; Immobilization; Porous supports; Protein distribution

Indexed keywords

AGAROSE; APPLIED POTENTIALS; COIMMOBILIZATION; CONFOCAL LASER SCANNING MICROSCOPY; CROSSLINKED; FLUORESCENT PROTEIN; GLYOXYL; HETEROGENEOUS DISTRIBUTIONS; HOMOGENEOUS DISTRIBUTION; IMIDAZOL; IMMOBILIZATION PROCESS; IMMOBILIZATION RATES; MATRIX; PHYSICO-CHEMICALS; POROUS MATRIXS; POROUS SOLIDS; POROUS SUPPORT; POROUS SURFACE; PRIMARY AMINE GROUPS; PROTEIN IMMOBILIZATION; PROTEIN LOCALIZATION; REACTIVE GROUP;

AMINO ACIDS; CHELATION; CONFOCAL MICROSCOPY; CROSSLINKING; FLUORESCENCE; LASER APPLICATIONS; PROTEINS;

ENZYME IMMOBILIZATION;

AGAROSE; FLUORESCENT PROTEIN; GLYOXYLIC ACID; IMIDAZOLE; PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CELLULAR DISTRIBUTION; CHEMICAL INTERACTION; CONFOCAL LASER MICROSCOPY; DENSITY GRADIENT; PRIORITY JOURNAL; PROTEIN IMMOBILIZATION; SURFACE PROPERTY;

ESCHERICHIA COLI; GREEN FLUORESCENT PROTEINS; HISTIDINE; IMIDAZOLES; IMMOBILIZED PROTEINS; KINETICS; MICROSPHERES; MODELS, THEORETICAL; POROSITY; SEPHAROSE;

EID: 80052392558     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2011.07.039     Document Type: Article

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