One-step enzyme extraction and immobilization for biocatalysis applications

Biotechnology Journal

Volumn 6, Issue 4, 2011, Pages 463-469

  Cassimjee, Karim Engelmark ^a   Kourist, Robert ^b   Lindberg, Diana ^c   Wittrup Larsen, Marianne ^a   Thanh, Nguyen Hong ^a   Widersten, Mikael ^c   Bornscheuer, Uwe T ^b   Berglund, Per ^a  

^a ROYAL INSTITUTE OF TECHNOLOGY   (Sweden)

^b UNIVERSITY OF GREIFSWALD   (Germany)

^c UPPSALA UNIVERSITY   (Sweden)

Author keywords

Condition promiscuity; Enzymatic synthesis; Enzyme in organic solvent; HIs6 tag; HisSi Immobilization

Indexed keywords

ANTARCTICA; AQUEOUS BUFFER; BACILLUS SUBTILIS; BIOCATALYSIS; CANDIDA ANTARCTICA LIPASE B; CONDITION PROMISCUITY; DIRECT EXTRACTION; ENZYMATIC SYNTHESIS; EPOXIDE HYDROLASES; ESTER HYDROLYSIS; HIS6-TAG; HISSI IMMOBILIZATION; IMMOBILIZATION METHOD; IMMOBILIZED ENZYME; LIPASE A; PSEUDOMONAS FLUORESCENCE; SILICA OXIDE; SOLANUM TUBEROSUM; SPECIFIC LOCATION;

ACETONITRILE; ADSORPTION; BACILLI; BACTERIOLOGY; CATALYSTS; ENZYME ACTIVITY; ENZYMES; ESTERS; HYDROLASES; METAL IONS; ORGANIC SOLVENTS; SILICA; SYNTHESIS (CHEMICAL);

ENZYME IMMOBILIZATION;

ACETONITRILE; ACID LIPASE; COBALT; CYCLOHEXANE; ESTERASE; HISTIDINE; LIPASE B; METAL ION; ORGANIC SOLVENT; SILICON DIOXIDE;

ARTICLE; BACILLUS SUBTILIS; BIOCATALYSIS; CANDIDA ANTARCTICA; CYTOPLASM; ENZYME ACTIVITY; EXTRACTION; METHODOLOGY; NONHUMAN; POTATO; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN IMMOBILIZATION; PSEUDOMONAS FLUORESCENS;

BACTERIAL PROTEINS; ENZYMES, IMMOBILIZED; EPOXIDE HYDROLASES; LIPASE; PLANT PROTEINS;

BACILLUS SUBTILIS; CANDIDA ANTARCTICA; PSEUDOMONAS; SOLANUM TUBEROSUM;

EID: 79953685174     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201000357     Document Type: Article

References (28)

1. Pollard, D. J., Woodley, J. M., Biocatalysis for pharmaceutical intermediates: The future is now. Trends Biotechnol. 2007, 25, 66-73.
2. Hult, K., Berglund, P., Enzyme promiscuity: Mechanism and applications. Trends Biotechnol. 2007, 25, 231-238.
3. Kazlauskas, R. J., Bornscheuer, U. T., Finding better protein engineering strategies. Nat. Chem. Biol. 2009, 5, 526-529.
4. Bornscheuer, U. T., Immobilizing enzymes: How to create more suitable biocatalysts. Angew. Chem., Int. Ed. 2003, 42, 3336-3337.
5. Brady, D., Jordaan, J., Advances in enzyme immobilisation. Biotechnol. Lett. 2009, 31, 1639-1650.
6. Cao, L., Immobilised enzymes: Science or art? Curr. Opin. Chem. Biol. 2005, 9, 217-226.
7. 6-tagged enzyme: Alanine racemase in hydrophobic solvent. Biotechnol. Bioeng. 2008, 99, 712-716.
8. 6-tagged protein onto nickel(II)-cyclam grafted mesoporous silica. Chem. Commun. (Cambridge) 2010, 46, 1124-1126.
9. Larsen, M. W., Bornscheuer, U. T., Hult, K., Expression of Candida antarctica lipase B in Pichia pastoris and various Escherichia coli systems. Protein Expression Purif. 2008, 62, 90-97.
10. Schmidt, M., Hasenpusch, D., Kahler, M., Kirchner, U. et al., Directed evolution of an esterase from Pseudomonas fluorescens yields a mutant with excellent enantioselectivity and activity for the kinetic resolution of a chiral building block. ChemBioChem 2006, 7, 805-809.
11. Heinze, B., Kourist, R., Fransson, L., Hult, K., Bornscheuer, U. T., Highly enantioselective kinetic resolution of two tertiary alcohols using mutants of an esterase from Bacillus subtilis. Protein Eng. Des. Sel. 2007, 20, 125-131.
12. Elfström, L. T., Widersten, M., Catalysis of potato epoxide hydrolase, StEH1. Biochem. J. 2005, 390, 633-640.
13. Droge, M. J., Boersma, Y. L., van Pouderoyen, G., Vrenken, T. E. et al., Directed evolution of Bacillus subtilis lipase A by use of enantiomeric phosphonate inhibitors: Crystal structures and phage display selection. ChemBioChem 2006, 7, 149-157.
14. Magnusson, A. O., Rotticci-Mulder, J. C., Santagostino, A., Hult, K., Creating space for large secondary alcohols by rational redesign of Candida antarctica lipase B. ChemBioChem 2005, 6, 1051-1056.
15. Chen, C. S., Fujimoto, Y., Girdaukas, G., Sih, C. J., Quantitative-analyses of biochemical kinetic resolutions of enantiomers. J. Am. Chem. Soc. 1982, 104, 7294-7299.
16. Uppenberg, J., Öhrner, N., Norin, M., Hult, K. et al., Crystallographic and molecular-modeling studies of lipase B from Candida antarctica reveal a stereospecificity pocket for secondary alcohols. Biochemistry 1995, 34, 16838-16851.
17. Blank, K., Morfill, J., Gumpp, H., Gaub, H. E., Functional expression of Candida antarctica lipase B in Eschericha coli. J. Biotechnol. 2006, 125, 474-483.
18. Bornscheuer, U. T., Bessler, C., Srinivas, R., Krishna, S. H., Optimizing lipases and related enzymes for efficient application. Trends Biotechnol. 2002, 20, 433-437.
19. Kourist, R., Bartsch, S., Fransson, L., Hult, K., Bornscheuer, U. T., Understanding promiscuous amidase activity of an esterase from Bacillus subtilis. ChemBioChem 2008, 9, 67-69.
20. Schmidt, M., Henke, E., Heinze, B., Kourist, R. et al., A versatile esterase from Bacillus subtilis: Cloning, expression, characterization, and its application in biocatalysis. Biotechnol. J. 2007, 2, 249-253.
21. Kourist, R., Bartsch, S., Bornscheuer, U. T., Highly enantioselective synthesis of arylaliphatic tertiary alcohols using mutants of an esterase from Bacillus subtilis. Adv. Synth. Catal. 2007, 349, 1393-1398.
22. Bartsch, S., Kourist, R., Bornscheuer, U. T., Complete inversion of enantioselectivity towards acetylated tertiary alcohols by a double mutant of a Bacillus subtilis esterase. Angew. Chem., Int. Ed. 2008, 47, 1508-1511.
23. Krebsfanger, N., Zocher, F., Altenbuchner, J., Bornscheuer, U. T., Characterization and enantioselectivity of a recombinant esterase from Pseudomonas fluorescens. Enzyme Microb. Technol. 1998, 22, 641-646.
24. Bornscheuer, U. T., Altenbuchner, J., Meyer, H. H., Directed evolution of an esterase for the stereoselective resolution of a key intermediate in the synthesis of epothilones. Biotechnol. Bioeng. 1998, 58, 554-559.
25. Park, S., Morley, K. L., Horsman, G. P., Holmquist, M. et al., Focusing mutations into the P. fluorescens esterase binding site increases enantioselectivity more effectively than distant mutations. Chem. Biol. 2005, 12, 45-54.
26. de Vries, E. J., Janssen, D. B., Biocatalytic conversion of epoxides. Curr. Opin. Biotechnol. 2003, 14, 414-420.
27. van Loo, B., Kingma, J., Arand, M., Wubbolts, M. G., Janssen, D. B., Diversity and biocatalytic potential of epoxide hydrolases identified by genome analysis. Appl. Environ. Microbiol. 2006, 72, 2905-2917.
28. Thomaeus, A., Naworyta, A., Mowbray, S. L., Widersten, M., Removal of distal protein-water hydrogen bonds in a plant epoxide hydrolase increases catalytic turnover but decreases thermostability. Protein Sci. 2008, 17, 1275-1284.