Generation of stable Drosophila cell lines using multicistronic vectors

Scientific Reports

Volumn 1, Issue , 2011, Pages

  González, Monika ^a   Martín Ruíz, Itziar ^a   Jiménez, Silvia ^a   Pirone, Lucia ^a   Barrio, Rosa ^a   Sutherland, James D ^a  

^a CIC BIOGUNE   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 84858204874     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep00075     Document Type: Article

References (51)

1. Hamburger, V. Wilhelm Roux: visionary with a blind spot. J Hist Biol 30, 229-238 (1997).
2. Cockrell, A. S. & Kafri, T. Gene delivery by lentivirus vectors. Mol Biotechnol 36, 184-204 (2007). (Pubitemid 350135911)
3. Jarvis, D. L. Baculovirus-insect cell expression systems. Methods Enzymol 463, 191-222 (2009).
4. Mohr, S., Bakal, C. & Perrimon, N. Genomic screening with RNAi: results and challenges. Annu Rev Biochem 79, 37-64 (2010).
5. Castoreno, A. B. et al. Small molecules discovered in a pathway screen target the Rho pathway in cytokinesis. Nat Chem Biol 6, 457-463 (2010).
6. Sorensen, H. P. Towards universal systems for recombinant gene expression. Microb Cell Fact 9, 27 (2010).
7. McCarroll, L. & King, L. A. Stable insect cell cultures for recombinant protein production. Curr Opin Biotechnol 8, 590-594 (1997). (Pubitemid 27455336)
8. Cherbas, L., Moss, R. & Cherbas, P. Transformation techniques for Drosophila cell lines. Methods Cell Biol 44, 161-179 (1994).
9. Van der Straten, A., Johansen, H., Rosenberg, M., Sweet, R. W. Introduction and constitutive expression of gene products in cultured Drosophila cells using hygromycin B selection. Methods in Molecular and Cellular Biology 1, 1-8 (1989).
10. Rio, D. C. & Rubin, G. M. Transformation of cultured Drosophila melanogaster cells with a dominant selectable marker. Mol Cell Biol 5, 1833-1838 (1985). (Pubitemid 15049140)
11. Bourouis, M. & Jarry, B. Vectors containing a prokaryotic dihydrofolate reductase gene transform Drosophila cells to methotrexate- resistance. Embo J 2, 1099-1104 (1983).
12. Makridou, P., Burnett, C., Landy, T. & Howard, K. Hygromycin B-selected cell lines from GAL4-regulated pUAST constructs. Genesis 36, 83-87 (2003). (Pubitemid 36818667)
13. Iwaki, T., Figuera, M., Ploplis, V. A. & Castellino, F. J. Rapid selection of Drosophila S2 cells with the puromycin resistance gene. Biotechniques 35, 482-484, 486 (2003).
14. Koelle, M. R. et al. The Drosophila EcR gene encodes an ecdysone receptor, a new member of the steroid receptor superfamily. Cell 67, 59-77 (1991). (Pubitemid 121001425)
15. Iwaki, T. & Castellino, F. J. A single plasmid transfection that offers a significant advantage associated with puromycin selection in Drosophila Schneider S2 cells expressing heterologous proteins. Cytotechnology 57, 45-49 (2008).
16. Segal, D., Cherbas, L. & Cherbas, P. Genetic transformation of Drosophila cells in culture by P element-mediated transposition. Somat Cell Mol Genet 22, 159-165 (1996).
17. Rees, S. et al. Bicistronic vector for the creation of stable mammalian cell lines that predisposes all antibiotic-resistant cells to express recombinant protein. Biotechniques 20, 102-104, 106, 108-110 (1996).
18. Gurtu, V., Yan, G. & Zhang, G. IRES bicistronic expression vectors for efficient creation of stable mammalian cell lines. Biochem Biophys Res Commun 229, 295-298 (1996). (Pubitemid 27013746)
19. Masoumi, A., Hanzlik, T. N. & Christian, P. D. Functionality of the 59-and intergenic IRES elements of cricket paralysis virus in a range of insect cell lines, and its relationship with viral activities. Virus Res 94, 113-120 (2003). (Pubitemid 36930034)
20. Cherry, S. & Perrimon, N. Entry is a rate-limiting step for viral infection in a Drosophila melanogaster model of pathogenesis. Nat Immunol 5, 81-87 (2004). (Pubitemid 38081472)
21. Carter, J. R., Fraser, T. S. & Fraser, M. J., Jr. Examining the relative activity of several dicistrovirus intergenic internal ribosome entry site elements in uninfected insect and mammalian cell lines. J Gen Virol 89, 3150-3155 (2008).
22. Mizuguchi, H., Xu, Z., Ishii-Watabe, A., Uchida, E. & Hayakawa, T. IRESdependent second gene expression is significantly lower than cap-dependent first gene expression in a bicistronic vector. Mol Ther 1, 376-382 (2000).
23. Underhill, M. F., Smales, C. M., Naylor, L. H., Birch, J. R. & James, D. C. Transient gene expression levels from multigene expression vectors. Biotechnol Prog 23, 435-443 (2007). (Pubitemid 46626319)
24. Ryan, M. D., King, A. M. & Thomas, G. P. Cleavage of foot-and-mouth disease virus polyprotein is mediated by residues located within a 19 amino acid sequence. J Gen Virol 72 (Pt 11), 2727-2732 (1991).
25. Donnelly, M. L. et al. Analysis of the aphthovirus 2A/2B polyprotein 'cleavage' mechanism indicates not a proteolytic reaction, but a novel translational effect: a putative ribosomal 'skip'. J Gen Virol 82, 1013-1025 (2001). (Pubitemid 32454602)
26. Donnelly, M. L. et al. The 'cleavage' activities of foot-and-mouth disease virus 2A site-directed mutants and naturally occurring '2A-like' sequences. J Gen Virol 82, 1027-1041 (2001). (Pubitemid 32454603)
27. de Felipe, P. et al. E unum pluribus: multiple proteins from a self-processing polyprotein. Trends Biotechnol 24, 68-75 (2006). (Pubitemid 43151978)
28. de Felipe, P. Skipping the co-expression problem: the new 2A "CHYSEL" technology. Genet Vaccines Ther 2, 13 (2004).
29. Szymczak, A. L. et al. Correction of multi-gene deficiency in vivo using a single 'self-cleaving' 2A peptide-based retroviral vector. Nat Biotechnol 22, 589-594 (2004). (Pubitemid 38585911)
30. Fang, J. et al. Stable antibody expression at therapeutic levels using the 2A peptide. Nat Biotechnol 23, 584-590 (2005). (Pubitemid 41724897)
31. Osborn, M. J. et al. A picornaviral 2A-like sequence-based tricistronic vector allowing for high-level therapeutic gene expression coupled to a dual-reporter system. Mol Ther 12, 569-574 (2005). (Pubitemid 41150144)
32. Provost, E., Rhee, J. & Leach, S. D. Viral 2A peptides allow expression of multiple proteins from a single ORF in transgenic zebrafish embryos. Genesis 45, 625-629 (2007). (Pubitemid 350099585)
33. Trichas, G., Begbie, J. & Srinivas, S. Use of the viral 2A peptide for bicistronic expression in transgenic mice. BMC Biol 6, 40 (2008).
34. Shaner, N. C. et al. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat Biotechnol 22, 1567-1572 (2004). (Pubitemid 40039460)
35. Coutts, A. S., MacKenzie, E., Griffith, E. & Black, D. M. TES is a novel focal adhesion protein with a role in cell spreading. J Cell Sci 116, 897-906 (2003). (Pubitemid 36367639)
36. Garvalov, B. K. et al. The conformational state of Tes regulates its zyxindependent recruitment to focal adhesions. J Cell Biol 161, 33-39 (2003). (Pubitemid 36459077)
37. Yanagawa, S., Lee, J. S. & Ishimoto, A. Identification and characterization of a novel line of Drosophila Schneider S2 cells that respond to wingless signaling. J Biol Chem 273, 32353-32359 (1998). (Pubitemid 29177184)
38. Schneider, I. Cell lines derived from late embryonic stages of Drosophila melanogaster. J Embryol Exp Morphol 27, 353-365 (1972).
39. Cherbas, P., Cherbas, L., Lee, S. S. & Nakanishi, K. 26-[125I]iodoponasterone A is a potent ecdysone and a sensitive radioligand for ecdysone receptors. Proc Natl Acad Sci USA 85, 2096-2100 (1988). (Pubitemid 24315835)
40. Carey, B. W. et al. Reprogramming of murine and human somatic cells using a single polycistronic vector. Proc Natl Acad Sci USA 106, 157-162 (2009).
41. Drusco, A. et al. Knockout mice reveal a tumor suppressor function for Testin. Proc Natl Acad Sci USA 102, 10947-10951 (2005). (Pubitemid 41105973)
42. Lycett, G. J. & Crampton, J. M. Stable transformation of mosquito cell lines using a hsp70:neo fusion gene. Gene 136, 129-136 (1993). (Pubitemid 24049231)
43. Zhao, Y. G. & Eggleston, P. Comparative analysis of promoters for transient gene expression in cultured mosquito cells. Insect Mol Biol 8, 31-38 (1999). (Pubitemid 29032133)
44. de Felipe, P., Luke, G. A., Brown, J. D. & Ryan, M. D. Inhibition of 2A-mediated 'cleavage' of certain artificial polyproteins bearing N-terminal signal sequences. Biotechnol J 5, 213-223 (2010).
45. de la Luna, S., Soria, I., Pulido, D., Ortin, J. & Jimenez, A. Efficient transformation of mammalian cells with constructs containing a puromycin-resistance marker. Gene 62, 121-126 (1988). (Pubitemid 18062011)
46. Kimura, M., Takatsuki, A. & Yamaguchi, I. Blasticidin S deaminase gene from Aspergillus terreus (BSD): a new drug resistance gene for transfection of mammalian cells. Biochim Biophys Acta 1219, 653-659 (1994). (Pubitemid 24353383)
47. Eulalio, A., Huntzinger, E. & Izaurralde, E. GW182 interaction with Argonaute is essential for miRNA-mediated translational repression and mRNA decay. Nat Struct Mol Biol 15, 346-353 (2008). (Pubitemid 351483394)
48. Cherbas, L., Koehler, M. M. & Cherbas, P. Effects of juvenile hormone on the ecdysone response of Drosophila Kc cells. Dev Genet 10, 177-188 (1989).
49. Cherbas, L., Yonge, C. D., Cherbas, P. & Williams, C. M. The morphological response of Kc-H cells to ecdysteroids: Hormonal specificity. Roux's Archives of Developmental Biology 189, 1 (1980).
50. Buster, D. W., Nye, J., Klebba, J. E. & Rogers, G. C. Preparation of Drosophila S2 cells for light microscopy. J Vis Exp (2010).
51. Sanchez, J. et al. Sumoylation modulates the activity of Spalt-like proteins during wing development in Drosophila. J Biol Chem 285, 25841-25849 (2010).