A picornaviral 2A-like sequence-based tricistronic vector allowing for high-level therapeutic gene expression coupled to a dual-reporter system

Molecular Therapy

Volumn 12, Issue 3, 2005, Pages 569-574

  Osborn, Mark J ^a   Panoskaltsis Mortari, Angela ^a   McElmurry, Ron T ^a   Bell, Scott K ^a   Vignali, Dario A A ^b   Ryan, Martin D ^c   Wilber, Andrew C ^a   McIvor, R Scott ^a   Tolar, Jakub ^a   Blazar, Bruce R ^a  

^a UNIVERSITY OF MINNESOTA   (United States)

^b ST JUDE CHILDREN S RESEARCH HOSPITAL   (United States)

^c UNIVERSITY OF ST ANDREWS   (United Kingdom)

Author keywords

Cis acting hydrolase elements; Iduronidase; Internal ribosome entry site; Mucopolysaccharidosis type I; Multicistronic; Whole body imaging

Indexed keywords

GENE PRODUCT; HYBRID PROTEIN; LEVO IDURONIDASE; LUCIFERASE; PROTEIN DSRED2; UNCLASSIFIED DRUG; VIRUS VECTOR;

AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL TRANSPORT; CONTROLLED STUDY; DRUG ACTIVITY; GENE CONSTRUCT; GENE EXPRESSION REGULATION; HUMAN; HURLER SYNDROME; IN VITRO STUDY; IN VIVO STUDY; INTERNAL RIBOSOME ENTRY SITE; MOUSE; NONHUMAN; PICORNAVIRUS; PROMOTER REGION; PROTEIN DEGRADATION; PROTEIN FUNCTION; REPORTER GENE; VIRAL GENE DELIVERY SYSTEM;

ANIMALS; CYSTEINE ENDOPEPTIDASES; GENE EXPRESSION; GENE THERAPY; GENE TRANSFER TECHNIQUES; GENES; GENES, REPORTER; GENETIC VECTORS; IDURONIDASE; LUCIFERASES; LUCIFERASES, FIREFLY; LUMINESCENT PROTEINS; MICE; MICE, INBRED NOD; MICE, SCID; MODELS, GENETIC; MUCOPOLYSACCHARIDOSES; NIH 3T3 CELLS; PICORNAVIRIDAE; PLASMIDS; PROTEIN BIOSYNTHESIS; TIME FACTORS; TRANSFECTION; VIRAL PROTEINS;

PICORNAVIRIDAE;

EID: 23844466445     PISSN: 15250016     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ymthe.2005.04.013     Document Type: Article

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