Preparation of Drosophila S2 cells for light microscopy

Journal of Visualized Experiments

Volumn , Issue 40, 2010, Pages

  Buster, Daniel W ^a   Nye, Jonathan ^a   Klebba, Joseph E ^a   Rogers, Gregory C ^a  

^a UNIVERSITY OF ARIZONA   (United States)

Author keywords

Cellular biology; Drosophila; dsRNA mediated interference; Issue 40; RNAi; S2 cells; Schneider cells

Indexed keywords

EID: 80355135387     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/1982     Document Type: Article

References (4)

1. Schneider, I. Cell lines derived from late embryonic stages of Drosophila melanogaster. J Embryol Exp Morphol 27, 353-365, (1972).
2. Rogers, S. L. & Rogers, G. C. Culture of Drosophila S2 cells and their use for RNAi-mediated loss-of-function studies and immunofluorescence microscopy. Nat Protoc 3, 606-611, (2008).
3. Rogers, S. L., Rogers, G. C., Sharp, D. J. & Vale, R. D. Drosophila EB1 is important for proper assembly, dynamics, and positioning of the mitotic spindle. J Cell Biol 158, 873-884, (2002).
4. Goshima, G. et al. Genes required for mitotic spindle assembly in Drosophila S2 cells. Science 316, 417-421, (2007).