Stable insect cell cultures for recombinant protein production

Current Opinion in Biotechnology

Volumn 8, Issue 5, 1997, Pages 590-594

  McCarroll, Lynn ^a   King, Linda A ^a  

^a OXFORD BROOKES UNIVERSITY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

MEMBRANE RECEPTOR; RECOMBINANT PROTEIN;

CELL CULTURE; DROSOPHILA; EXPRESSION VECTOR; GENETIC TRANSFORMATION; INSECT; LEPIDOPTERA; MALARIA; MOSQUITO; NONHUMAN; PRIORITY JOURNAL; PROTEIN SYNTHESIS; RNA TRANSLATION; SHORT SURVEY;

INSECTA; LEPIDOPTERA;

EID: 0030687630     PISSN: 09581669     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0958-1669(97)80034-1     Document Type: Article

References (34)

1. King LA, Possee RD: The baculovirus expression system: a laboratory manual. London: Chapman and Hall; 1992.
2. Sondergaard L: Drosophila cells can be grown to high cell densities in a bioreactor. Biotechnology techniques 1996, 10:161-166.
3. Miller LK: Insect viruses. In Fields Virology, edn 3. Edited by Fields BN, Knipe DM, Howley PM. New York: Lippincott-Raven Publishers; 1996:533-556.
4. Bourouis M, Jarry B: Vectors containing a prokaryotic dihydrofolate reductase gene transform Drosophila cells to methotrexate-resistance. EMBO J 1983, 2:1099-1104.
5. Jarvis DL, Fleming JGW, Kovacs GR, Summers MD, Guarino LA: Use of early baculovirus promoters for continuous expression and efficient processing of foreign gene products in stably-transformed lepidopteran insect cells. Bio-Technology 1990, 8:50-55.
6. Jarvis DL, Finn EE: Modifying the insect cell N-glycosylation pathway with immediate early baculovirus expression vectors. Nat Biotechnol 1996, 14:1288-1292.
7. Joyce KA, Atkinson AE, Bermudez I, Beadle DJ, King LA: Synthesis of functional GABA-A receptors in stable insect cell lines. FEBS Lett 1993, 335:61-64.
8. Henderson J, Atkinson AE, Lazarus CM, Hawes CR, Napier RM, Macdonald H, King LK: Stable expression of maize auxin-binding protein in insect cell lines. FEBS Lett 1995, 371:293-296.
9. Brighty DW, Rosenberg M, Chen ISY, Ivey-Hoyle M: Envelope proteins from isolates of human immunodeficiency virus type 1 that are refractory to neutralisation by soluble CD4 possess high affinity for the CD4 receptor. Proc Natl Acad Sci USA 1991, 88:7802-7805.
10. Shotkoski F, Zhang HG, Jackson MB, French-Constant RH: Stable expression of insect GABA receptors in insect cell lines; promoters for efficient expression of Drosophila and mosquito RdI GABA receptors in stably transformed mosquito cell lines. FEBS Lett 1996, 380:257-262. Recombinant Drosophila and mosquito cell lines that express an insect γ-aminobutyric acid RdI subunit are described. Expression is directed by the high copy numbers of the ie-1 promoter, which appears to work efficiently across insect species.
11. Buckingham SD, Matsuda K, Hosie AM, Baylis HA, Squire MD, Lansdell SJ, Millar NS, Satelle DB: Wild-type and insecticide-resistant homo-oligomeric GABA receptors of Drosophila melanogaster stably expressed in a Drosophila cell line. Neurophramacology 1996, 35:1393-1401. A comparison of recombinant insect GABA RdI subunit expression in Drosophila cell lines and Xenopus oocytes.
12. Ashburner M: Drosophila, A Laboratory Handbook. New York: Cold Spring Harbour Press; 1989.
13. Cherbas L, Moss R, Cherbas P: Transformation techniques for Drosophila cell lines. Methods Cell Biol 1994, 44:161-179.
14. Segal D, Cherbas L, Cherbas P: Genetic transformation of Drosophila cells in culture by P element-mediated transposition. Somatic Cell Mol Genet 1996, 22:159-165. A novel method of DNA transformation is described using P element transposition. The use of the P element results in useful levels of expression in Drosophila cells.
15. Miller LH, Sakai RK, Romans P, Gwadz RW, Kantoff P, Coon HG: Stable integration and expression of a bacterial gene in the mosquito Anopheles gambiae. Science 1987, 237:779-781.
16. Monroe TJ, Muhlmann-Diaz MC, Kovach MJ, Carlson JO, Bedford JS, Beaty BJ: Stable transformation of a mosquito cell line results in extraordinarily high copy numbers of the plasmid. Proc Natl Acad Sci USA 1992, 89:5725-5729.
17. Lycett GJ, Crampton JM: Stable transformation of mosquito cell lines using a hsp70::neo fusion gene. Gene 1993, 136:129-136.
18. Jarvis DL: Continuous foreign gene expression in stably-transformed insect cells. In Insect Cell Culture Engineering. Edited by Goosen MFA, Daugulis A, Faulkner P. New York: Marcel Dekker; 1993:193-217.
19. Jarvis DL, Guarino LA: Continuous foreign gene expression in transformed lepidopteran insect cells. In Methods in Molecular Biology 1995, 39:187-201.
20. Atkinson AE, Henderson J, Hawes CR, King LA: Efficient membrane targeting of the chick nicotinic acetylcholine receptor α-subunit in stable insect cell lines. Cytotechnology 1996, 19:37-42. This report demonstrates the potential for stable lepidopteran insect cell lines in the expression of membrane-targeted receptor subunits.
21. Kleymann G, Boege F, Hahn M, Hampe M, Vasudevan S, Reilander H: Human β2-adrenergic receptor produced in stably transformed insect cells is functionally coupled via endogenous GTP-binding protein to adenylyl cyclase. Eur J Biochem 1993, 213:797-804.
22. Jarvis DL, Weinkauf C, Guarino LA: Immediate early baculovirus vectors for foreign gene expression in transformed or infected insect cells. Protein Expand Purif 1996, 8:193-203.
23. Urlaub G, Chasin LA: Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activity. Proc Natl Acad Sci USA 1980, 77:4216-4220.
24. Di Nocera PP, Dawid IB: Transient expression of genes introduced into cultured cells of Drosophila. Proc Natl Acad Sci USA 1983, 80:7095-7098.
25. Vanden Broeck J, Vulsteke V, Huybrechts R, De Loof A: Characterisation of a cloned locust tyramine receptor cDNA by functional expression in permanently transformed Drosophila 52 cells. J Neurochem 1995, 64:2387-2395.
26. Li B, Tsing S, Kosaka AH, Nguyen B, Osen EG, Bach C, Barnett J: Expression of human dopamine β-hydroxylase in Drosophila Schneider 2 cells. Biochem J 1996, 313:57-64. Receptor subunits are shown to be functional, correctly processed and synthesised to relatively high levels in stably transformed insect cells.
27. Van der Straten A, Johansen H, Rosenberg M, Sweet RW: Introduction and constitutive expression of gene products in cultured Drosophila cells using hygromycin B selection. Methods Mol Cell Biol 1989, 1:1-8.
28. Johansen H, van der Straten A, Sweet R, Otto E, Maroni G, Rosenberg M: Regulated expression at high copy number allows production of a growth-inhibitory oncogene product in Drosophila Schneider cells. Genes Dev 1989, 3:882-889.
29. Ivey-Hoyle M, Culp JS, Chaikin MA, Hellmig BD, Matthews TJ, Sweet RW, Rosenberg M: Envelope glycoproteins from biologically diverse isolates of immunodeficiency viruses have widely different affinities for CD4. Proc Natl Acad Sci USA 1991, 88:513-516.
30. Ivey-Hoyle M, Clark RK, Rosenberg M: The N-terminal 31 amino acids of human immunodeficiency virus type 1 envelope protein gp120 contain a potential gp41 contact site. J Virol 1991, 65:2682-2685.
31. Millar NS, Buckingham SD, Sattelle DB: Stable expression of a functional homo-oligomeric Drosophila GABA receptor in a Drosophila cell line. Proc R Soc Lond Biol Sci 1994, 258:307-314.
32. Millar NS, Baylis HA, Reaper C, Bunting R, Mason WT, Sattelle DB: Functional expression of a cloned Drosophila muscarinic acetylcholine receptor in a stable Drosophila cell line. J Exp Biol 1995, 198:1843-1850.
33. Butler D, Maurice J, O'Brien C: Briefing: malaria, Nature 1997, 386:535-540. A review of events at the recent world malaria conference. An interesting article on the potential of stably transformed insect cells for the control of vector-borne diseases.
34. Johansen HR, van der Straten-Ponthoz AA, Rosenberg M: Expression of foreign genes in Drosophila cells. European Patent Application Number 88304093.3, publication number 0 290 261 A1 1988.