Viral 2A peptides allow expression of multiple proteins from a single ORF in transgenic zebrafish embryos

Genesis (United States)

Volumn 45, Issue 10, 2007, Pages 625-629

  Provost, Elayne ^a   Rhee, Jerry ^a   Leach, Steven D ^a,b  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b JOHNS HOPKINS UNIVERSITY   (United States)

Author keywords

2A self cleaving peptide; IRES; Multicistronic; Transgenesis; Zebrafish

Indexed keywords

VIRUS PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; EMBRYO; FLUORESCENCE MICROSCOPY; GENE EXPRESSION; IMMUNOBLOTTING; NONHUMAN; PEPTIDE ANALYSIS; PRIORITY JOURNAL; STOICHIOMETRY; TRANSGENE; ZEBRA FISH;

DANIO RERIO;

EID: 36048993980     PISSN: 1526954X     EISSN: 1526968X     Source Type: Journal    
DOI: 10.1002/dvg.20338     Document Type: Article

References (14)

1. de Felipe P, Luke GA, Hughes LE, Gani D, Halpin C, Ryan MD. 2006. E unum pluribus: Multiple proteins from a self-processing polyprotein. Trends Biotechnol 24:68-75.
2. Donnelly ML, Hughes LE, Luke G, Mendoza H, ten Dam E, Gani D, Ryan MD. 2001a. The 'cleavage' activities of foot-and-mouth disease virus 2A site-directed mutants and naturally occurring '2A-like' sequences. J Gen Virol 82:1027-1041.
3. Donnelly ML, Luke G, Mehrotra A, Li X, Hughes LE, Gani D, Ryan MD. 2001b. Analysis of the aphthovirus 2A/2B polyprotein 'cleavage' mechanism indicates not a proteolytic reaction, but a novel translational effect: A putative ribosomal 'skip'. J Gen Virol 82:1013-1025.
4. Fisher S, Grice EA, Vinton RM, Bessling SL, McCallion AS. 2006. Conservation of RET regulatory function from human to zebrafish without sequence similarity. Science 312:276-279.
5. Godinho L, Mumm JS, Williams PR, Schroeter EH, Koerber A, Park SW, Leach SD, Wong RO. 2005. Targeting of amacrine cell neurites to appropriate synaptic laminae in the developing zebrafish retina. Development 132:5069-5079.
6. Holst J, Vignali KM, Burton AR, Vignali DA. 2006. Rapid analysis of T-cell selection in vivo using T cell-receptor retrogenic mice. Nat Methods 3:191-197.
7. Kawakami K, Shima A. 1999. Identification of the Tol2 transposase of the medaka fish Oryzias latipes that catalyzes excise on of a non-autonomous Tol2 element in zebrafish Danio rerio. Gene 240:239-244.
8. Kawakami K, Shima A, Kawakami N. 2000. Identification of a functional transposase of the Tol2 element, an Ac-like element from the Japanese medaka fish, and its transposition in the zebrafish germ lineage. Proc Natl Acad Sci USA 97:11403-11408.
9. Kawakami K, Takeda H, Kawakami N, Kobayashi M, Matsuda N, Mishina M. 2004. A transposon-mediated gene trap approach identifies developmentally regulated genes in zebrafish. Dev Cell 7:133-144.
10. Mizuguchi H, Xu Z, Ishii-Watabe A, Uchida E, Hayakawa T. 2000. IRES-dependent second gene expression is significantly lower than cap-dependent first gene expression in a bicistronic vector. Mol Ther 1:376-382.
11. Pisharath H, Rhee JM, Swanson MA, Leach SD, Parsons MJ. 2007. Targeted ablation of beta cells in the embryonic zebrafish pancreas using E. coli nitroreductase. Mech Dev 124:218-229.
12. Ryan MD, Drew J. 1994. Foot-and-mouth disease virus 2A oligopeptide mediated cleavage of an artificial polyprotein. EMBO J 13:928-933.
13. Ryan MD, King AM, Thomas GP. 1991. Cleavage of foot-and-mouth disease virus polyprotein is mediated by residues located within a 19 amino acid sequence. J Gen Virol 72 (Part 11):2727-2732.
14. Szymczak AL, Workman CJ, Wang Y, Vignali KM, Dilioglou S, Vanin EF, Vignali DA. 2004. Correction of multi-gene deficiency in vivo using a single 'self-cleaving' 2A peptide-based retroviral vector. Nat Biotechnol 22:589-594.