Rapid, efficient functional characterization and recovery of HIV-specific human CD8 + T cells using microengraving

Proceedings of the National Academy of Sciences of the United States of America

Volumn 109, Issue 10, 2012, Pages 3885-3890

  Varadarajan, Navin ^a,e   Kwon, Douglas S ^b,c   Law, Kenneth M ^b   Ogunniyi, Adebola O ^a   Anahtar, Melis N ^b   Richter, James M ^c   Walker, Bruce D ^b,d   Love, J Christopher ^a,b  

^a Massachusetts Institute of Technology Cambridge   (United States)

^b HARVARD UNIVERSITY   (United States)

^c MASSACHUSETTS GENERAL HOSPITAL   (United States)

^d HOWARD HUGHES MEDICAL INSTITUTE   (United States)

^e UNIVERSITY OF HOUSTON   (United States)

Author keywords

Microarrays; Nanowells; Single cell analysis; Soft lithography; T cell cloning

Indexed keywords

ANTIGEN SPECIFICITY; ARTICLE; BLOOD CELL; CD8+ T LYMPHOCYTE; CLONAL VARIATION; CONTROLLED STUDY; CYTOKINE RELEASE; CYTOMETRY; ENZYME LINKED IMMUNOSPOT ASSAY; GENOTYPE; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS; INTERMETHOD COMPARISON; LYMPHOCYTE FUNCTION; MICROENGRAVING; MICROMANIPULATION; MUCOSA; PRIORITY JOURNAL; STAINING;

ANTIGENS, CD3; CD8-POSITIVE T-LYMPHOCYTES; CLONING, MOLECULAR; ENZYME-LINKED IMMUNOSORBENT ASSAY; EPITOPES; GENOTYPE; HIV; HIV INFECTIONS; HLA ANTIGENS; HUMANS; INTERFERON-GAMMA; LAB-ON-A-CHIP DEVICES; MICROMANIPULATION; NANOTECHNOLOGY; T-LYMPHOCYTES;

EID: 84857978200     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1111205109     Document Type: Article

References (42)

1. Robins HS, et al. (2010) Overlap and effective size of the human CD8+ T cell receptor repertoire. Sci Transl Med 2(47):47-64.
2. Betts MR, et al. (2001) Analysis of total human immunodeficiency virus (HIV)-specific CD4(+) and CD8(+) T-cell responses: Relationship to viral load in untreated HIV infection. J Virol 75:11983-11991. (Pubitemid 33115870)
3. Ellis JM, et al. (2000) Frequencies of HLA-A2 alleles in five U.S. population groups. Predominance Of A*02011 and identification of HLA-A*0231. Hum Immunol 61:334-340. (Pubitemid 30117803)
4. Streeck H, Frahm N, Walker BD (2009) The role of IFN-gamma Elispot assay in HIV vaccine research. Nat Protoc 4:461-469.
5. Altman JD, Davis MM (2003) MHC-peptide tetramers to visualize antigen-specific T cells. Curr Protoc Immunol 53:17.3.1-17.3.33.
6. Toebes M, Rodenko B, Ovaa H, Schumacher TN (2009) Generation of peptide MHC class I monomers and multimers through ligand exchange. Curr Protoc Immunol 87:18.16.1-18.16.20.
7. Rodenko B, et al. (2006) Generation of peptide-MHC class I complexes through UVmediated ligand exchange. Nat Protoc 1:1120-1132.
8. Subirá D, et al. (2002) Flow cytometric analysis of cerebrospinal fluid samples and its usefulness in routine clinical practice. Am J Clin Pathol 117:952-958. (Pubitemid 34564353)
9. Martin-Hirsch P, Jarvis G, Kitchener H, Lilford R (2000) Collection devices for obtaining cervical cytology samples. Cochrane Database Syst Rev (3):CD001036.
10. Li Pira G, Ivaldi F, Bottone L, Manca F (2007) High throughput functional microdissection of pathogen-specific T-cell immunity using antigen and lymphocyte arrays. J Immunol Methods 326:22-32. (Pubitemid 47361195)
11. Hoff A, et al. (2010) Peptide microarrays for the profiling of cytotoxic T-lymphocyte activity using minimum numbers of cells. Cancer Immunol Immunother 59:1379-1387.
12. Yue C, Oelke M, Paulaitis ME, Schneck JP (2010) Novel cellular microarray assay for profiling T-cell peptide antigen specificities. J Proteome Res 9:5629-5637.
13. Bradshaw EM, et al. (2008) Concurrent detection of secreted products from human lymphocytes by microengraving: Cytokines and antigen-reactive antibodies. Clin Immunol 129:10-18.
14. Han Q, Bradshaw EM, Nilsson B, Hafler DA, Love JC (2010) Multidimensional analysis of the frequencies and rates of cytokine secretion from single cells by quantitative microengraving. Lab Chip 10:1391-1400.
15. + T cell functions reveals discordance for cytokine secretion and cytolysis. J Clin Invest 121:4322-4331.
16. Deeks SG, Walker BD (2007) Human immunodeficiency virus controllers: Mechanisms of durable virus control in the absence of antiretroviral therapy. Immunity 27:406-416. (Pubitemid 47418678)
17. Koup RA, et al. (1994) Temporal association of cellular immune responses with the initial control of viremia in primary human immunodeficiency virus type 1 syndrome. J Virol 68:4650-4655. (Pubitemid 24183063)
18. Borrow P, Lewicki H, Hahn BH, Shaw GM, Oldstone MB (1994) Virus-specific CD8+ cytotoxic T-lymphocyte activity associated with control of viremia in primary human immunodeficiency virus type 1 infection. J Virol 68:6103-6110. (Pubitemid 24258634)
19. Dyer WB, et al. (2008) Mechanisms of HIV non-progression; robust and sustained CD4+ T-cell proliferative responses to p24 antigen correlate with control of viraemia and lack of disease progression after long-term transfusion-acquired HIV-1 infection. Retrovirology 5:112.
20. Pereyra F, et al.; International HIV Controllers Study (2010) The major genetic determinants of HIV-1 control affect HLA class I peptide presentation. Science 330:1551-1557.
21. Chen H, et al. (2009) Differential neutralization of human immunodeficiency virus (HIV) replication in autologous CD4 T cells by HIV-specific cytotoxic T lymphocytes. J Virol 83:3138-3149.
22. Sun Y, et al. (2003) A systematic comparison of methods to measure HIV-1 specific CD8 T cells. J Immunol Methods 272:23-34. (Pubitemid 36050742)
23. Zuñiga R, et al. (2006) Relative dominance of Gag p24-specific cytotoxic T lymphocytes is associated with human immunodeficiency virus control. J Virol 80:3122-3125. (Pubitemid 43346412)
24. Kiepiela P, et al. (2007) CD8+ T-cell responses to different HIV proteins have discordant associations with viral load. Nat Med 13:46-53. (Pubitemid 46067385)
25. Moss ML, et al. (1997) Cloning of a disintegrin metalloproteinase that processes precursor tumour-necrosis factor-alpha. Nature 385:733-736. (Pubitemid 27098095)
26. Haney D, et al. (2011) Isolation of viable antigen-specific CD8+ T cells based on membrane-bound tumor necrosis factor (TNF)-α expression. J Immunol Methods 369:33-41.
27. Song Q, et al. (2010) On-chip activation and subsequent detection of individual antigen- specific T cells. Anal Chem 82:473-477.
28. Choi JH, et al. (2010) Development and optimization of a process for automated recovery of single cells identified by microengraving. Biotechnol Prog 26:888-895.
29. Karlsson AC, et al. (2003) Comparison of the ELISPOT and cytokine flow cytometry assays for the enumeration of antigen-specific T cells. J Immunol Methods 283:141-153. (Pubitemid 37501065)
30. Pahar B, Li J, Rourke T, Miller CJ, McChesney MB (2003) Detection of antigen-specific T cell interferon gamma expression by ELISPOT and cytokine flow cytometry assays in rhesus macaques. J Immunol Methods 282:103-115. (Pubitemid 37357037)
31. Maecker HT, et al. (2005) Impact of cryopreservation on tetramer, cytokine flow cytometry, and ELISPOT. BMC Immunol 6:17.
32. Han Q, et al. (2012) Polyfunctional responses by human T cells result from sequential release of cytokines. Proc Natl Acad Sci USA 109:1605-1612.
33. Schaubert KL, et al. (2010) Generation of robust CD8+ T-cell responses against subdominant epitopes in conserved regions of HIV-1 by repertoire mining with mimotopes. Eur J Immunol 40:1950-1962.
34. Addo MM, et al. (2003) Comprehensive epitope analysis of human immunodeficiency virus type 1 (HIV-1)-specific T-cell responses directed against the entire expressed HIV-1 genome demonstrate broadly directed responses, but no correlation to viral load. J Virol 77:2081-2092. (Pubitemid 36110021)
35. Dalod M, et al. (1999) Broad, intense anti-human immunodeficiency virus (HIV) ex vivo CD8(+) responses in HIV type 1-infected patients: Comparison with anti-Epstein-Barr virus responses and changes during antiretroviral therapy. J Virol 73:7108-7116. (Pubitemid 29383190)
36. Betts MR, et al. (2006) HIV nonprogressors preferentially maintain highly functional HIV-specific CD8+ T cells. Blood 107:4781-4789. (Pubitemid 43882629)
37. Migueles SA, et al. (2002) HIV-specific CD8+ T cell proliferation is coupled to perforin expression and is maintained in nonprogressors. Nat Immunol 3:1061-1068. (Pubitemid 35363659)
38. Sáez-Cirión A, et al. (2009) ANRS EP36 HIV Controllers Study Group (2009) Heterogeneity in HIV suppression by CD8 T cells from HIV controllers: Association with Gagspecific CD8 T cell responses. J Immunol 182:7828-7837.
39. Billam P, et al. (2011) T Cell receptor clonotype influences epitope hierarchy in the CD8+ T cell response to respiratory syncytial virus infection. J Biol Chem 286: 4829-4841.
40. Shacklett BL, et al. (2003) Optimization of methods to assess human mucosal T-cell responses to HIV infection. J Immunol Methods 279:17-31. (Pubitemid 37297487)
41. Han C, et al. (2010) Integration of single oocyte trapping, in vitro fertilization and embryo culture in a microwell-structured microfluidic device. Lab Chip 10:2848-2854.
42. Yang OO, et al. (1997) Suppression of human immunodeficiency virus type 1 replication by CD8+ cells: Evidence for HLA class I-restricted triggering of cytolytic and noncytolytic mechanisms. J Virol 71:3120-3128. (Pubitemid 27119959)