High throughput functional microdissection of pathogen-specific T-cell immunity using antigen and lymphocyte arrays

Journal of Immunological Methods

Volumn 326, Issue 1-2, 2007, Pages 22-32

  Li Pira, Giuseppina ^a   Ivaldi, Federico ^a   Bottone, Laura ^b   Manca, Fabrizio ^b  

^a ADVANCED BIOTECHNOLOGY CENTER   (Italy)

^b G GASLINI INSTITUTE   (Italy)

Author keywords

Cytokines; Epitope mapping; Fungal immunity; High throughput cellular assays; Human T cells; Immunodeficiency; Viral immunity

Indexed keywords

CYTOKINE;

ARTICLE; CELL CULTURE; CELLULAR IMMUNITY; CHILD; CONTROLLED STUDY; CYTOKINE RELEASE; CYTOMEGALOVIRUS; DATA ANALYSIS; DNA MICROARRAY; EPITOPE MAPPING; FUNGUS IMMUNITY; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; IMMUNE DEFICIENCY; IMMUNOSTIMULATION; LYMPHOCYTE; LYMPHOCYTE CULTURE; MICRODISSECTION; PERIPHERAL BLOOD MONONUCLEAR CELL; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; SENSITIVITY ANALYSIS; T LYMPHOCYTE; T LYMPHOCYTE ACTIVATION; VETERINARY MEDICINE; VIRUS IMMUNITY;

ANTIGENS, FUNGAL; ANTIGENS, PROTOZOAN; ANTIGENS, VIRAL; CELL CULTURE TECHNIQUES; CELL LINE; CELLS, CULTURED; CYTOKINES; ENZYME-LINKED IMMUNOSORBENT ASSAY; EPITOPES, T-LYMPHOCYTE; HUMANS; IMMUNOPHENOTYPING; T-LYMPHOCYTES; TISSUE ARRAY ANALYSIS;

EID: 34548403850     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2007.06.012     Document Type: Article

References (38)

1. Bakker A.H., and Schumacher T.N. MHC multimer technology: current status and future prospects. Curr. Opin. Immunol. 17 (2005) 428
2. Betts M.R., Nason M.C., West S.M., De Rosa S.C., Migueles S.A., Abraham J., Lederman M.M., Benito J.M., Goepfert P.A., Connors M., Roederer M., and Koup R.A. HIV nonprogressors preferentially maintain highly functional HIV-specific CD8+ T cells. Blood 107 (2006) 4781
3. Chattopadhyay P.K., Price D.A., Harper T.F., Betts M.R., Yu J., Gostick E., Perfetto S.P., Goepfert P., Koup R.A., De Rosa S.C., Bruchez M.P., and Roederer M. Quantum dot semiconductor nanocrystals for immunophenotyping by polychromatic flow cytometry. Nat. Med. 12 (2006) 972
4. Chen D.S., Soen Y., Stuge T.B., Lee P.P., Weber J.S., Brown P.O., and Davis M.M. Marked differences in human melanoma antigen-specific T cell responsiveness after vaccination using a functional microarray. PLoS Med. 2 (2005) e265
5. De Groot A.S. Immunomics: discovering new targets for vaccines and therapeutics. Drug Discov. Today 11 (2006) 203
6. De Rosa S.C., Lu F.X., Yu J., Perfetto S.P., Falloon J., Moser S., Evans T.G., Koup R., Miller C.J., and Roederer M. Vaccination in humans generates broad T cell cytokine responses. J. Immunol. 173 (2004) 5372
7. *0201 restricted peptide-based vaccine against human cytomegalovirus infection. Blood 90 (1997) 1751
8. +-T-cell responses to human cytomegalovirus reveals broad and multispecific reactivities in healthy virus carriers. J. Virol. 77 (2003) 5226
9. Fazekas de St G. The evaluation of limiting dilution assays. J. Immunol. Methods 49 (1982) R11
10. Gallot G., Vivien R., Ibisch C., Lule J., Davrinche C., Gaschet J., and Vie H. Purification of Ag-specific T lymphocytes after direct peripheral blood mononuclear cell stimulation followed by CD25 selection. I. Application to CD4(+) or CD8(+) cytomegalovirus phosphoprotein pp65 epitope determination. J. Immunol. 167 (2001) 4196
11. Gavin M.A., Gilbert M.J., Riddell S.R., Greenberg P.D., and Bevan M.J. Alkali hydrolysis of recombinant proteins allows for the rapid identification of class I MHC-restricted CTL epitopes. J. Immunol. 151 (1993) 3971
12. Gratama J.W., and Kern F. Flow cytometric enumeration of antigen-specific T lymphocytes. Cytometry A 58 (2004) 79
13. Hebart H., Daginik S., Stevanovic S., Grigoleit U., Dobler A., Baur M., Rauser G., Sinzger C., Jahn G., Loeffler J., Kanz L., Rammensee H.G., and Einsele H. Sensitive detection of human cytomegalovirus peptide-specific cytotoxic T-lymphocyte responses by interferon-gamma-enzyme-linked immunospot assay and flow cytometry in healthy individuals and in patients after allogeneic stem cell transplantation. Blood 99 (2002) 3830
14. Kern F., Surel I.P., Brock C., Freistedt B., Radtke H., Scheffold A., Blasczyk R., Reinke P., Schneider-Mergener J., Radbruch A., Walden P., and Volk H.D. T-cell epitope mapping by flow cytometry. Nat. Med. 4 (1998) 975
15. Kern F., Bunde T., Faulhaber N., Kiecker F., Khatamzas E., Rudawski I.M., Pruss A., Gratama J.W., Volkmer-Engert R., Ewert R., Reinke P., Volk H.D., and Picker L.J. Cytomegalovirus (CMV) phosphoprotein 65 makes a large contribution to shaping the T cell repertoire in CMV-exposed individuals. J. Infect. Dis. 185 (2002) 1709
16. Kern F., Li Pira G., Gratama J.W., Manca F., and Roederer M. Measuring Ag-specific immune responses: understanding immunopathogenesis and improving diagnostics in infectious disease, autoimmunity and cancer. Trends Immunol. 26 (2005) 477
17. Khanna R., and Diamond D.J. Human cytomegalovirus vaccine: time to look for alternative options. Trends Mol. Med. 12 (2006) 26
18. Khattab B.A., Lindenmaier W., Frank R., and Link H. Three T-cell epitopes within the C-terminal 265 amino acids of the matrix protein pp65 of human cytomegalovirus recognized by human lymphocytes. J. Med. Virol. 52 (1997) 68
19. *2402-restricted cytomegalovirus-specific CD8(+) T-cell epitopes by a computer algorithm and an enzyme-linked immunospot assay. Blood 98 (2001) 1872
20. Li Pira G., Bottone L., Ivaldi F., Pelizzoli R., Del Galdo F., Lozzi L., Bracci L., Loregian A., Palu G., De Palma R., Einsele H., and Manca F. Identification of new Th peptides from the cytomegalovirus protein pp65 to design a peptide library for generation of CD4 T cell lines for cellular immunoreconstitution. Int. Immunol. 16 (2004) 635
21. Li Pira G., Bottone L., Ivaldi F., Tagliamacco A., Fiordoro S., Ricciardi A., Barbano G., and Manca F. Generation of cytomegalovirus (CMV)-specific CD4 T cell lines devoid of alloreactivity, by use of a mixture of CMV-phosphoprotein 65 peptides for reconstitution of the T helper repertoire. J. Infect. Dis. 191 (2005) 215
22. Li Pira G., Kern F., Gratama J., Roederer M., and Manca F. Measurement of antigen specific immune responses: 2006 update. Clin. Cytom. 72 (2007) 77
23. Li Pira G., Ivaldi F., Bottone L., Koopman G., and Manca F. Helper function of cytolytic lymphocytes: switching roles in the immune response. Eur. J. Immunol. 37 (2007) 66
24. Longmate J., York J., La Rosa C., Krishnan R., Zhang M., Senitzer D., and Diamond D.J. Population coverage by HLA class-I restricted cytotoxic T-lymphocyte epitopes. Immunogenetics 52 (2001) 165
25. McKinney D.M., Skvoretz R., Qin M., Ishioka G., and Sette A. Characterization of an in situ IFN-gamma ELISA assay which is able to detect specific peptide responses from freshly isolated splenocytes induced by DNA minigene immunization. J. Immunol. Methods 237 (2000) 105
26. McMichael A.J., and Hanke T. HIV vaccines 1983-2003. Nat. Med. 9 (2003) 874
27. Perfetto S.P., Chattopadhyay P.K., and Roederer M. Seventeen-colour flow cytometry: unravelling the immune system. Nat. Rev. Immunol. 4 (2004) 648
28. Retiere C., Prod'homme V., Imbert-Marcille B.M., Bonneville M., Vie H., and Hallet M.M. Generation of cytomegalovirus-specific human T-lymphocyte clones by using autologous B-lymphoblastoid cells with stable expression of pp65 or IE1 proteins: a tool to study the fine specificity of the antiviral response. J. Virol. 74 (2000) 3948
29. Schepers K., Arens R., and Schumacher T.N. Dissection of cytotoxic and helper T cell responses. Cell. Mol. Life Sci. 62 (2005) 2695
30. Soen Y., Chen D.S., Kraft D.L., Davis M.M., and Brown P.O. Detection and characterization of cellular immune responses using peptide-MHC microarrays. PLoS Biol. 1 (2003) e65
31. Solache A., Morgan C.L., Dodi A.I., Morte C., Scott I., Baboonian C., Zal B., Goldman J., Grundy J.E., and Madrigal J.A. Identification of three HLA-A*0201-restricted cytotoxic T cell epitopes in the cytomegalovirus protein pp65 that are conserved between eight strains of the virus. J. Immunol. 163 (1999) 5512
32. Sospedra M., Pinilla C., and Martin R. Use of combinatorial peptide libraries for T-cell epitope mapping. Methods 29 (2003) 236
33. Stone J.D., Demkowicz Jr. W.E., and Stern L.J. HLA-restricted epitope identification and detection of functional T cell responses by using MHC-peptide and costimulatory microarrays. Proc. Natl. Acad. Sci. U. S. A. 102 (2005) 3744
34. Strijbosch L.W., Buurman W.A., Does R.J., Zinken P.H., and Groenewegen G. Limiting dilution assays. Experimental design and statistical analysis. J. Immunol. Methods 97 (1987) 133
35. Thiel A., Scheffold A., and Radbruch A. Antigen-specific cytometry - new tools arrived!. Clin. Immunol. 111 (2004) 155
36. Toebes M., Coccoris M., Bins A., Rodenko B., Gomez R., Nieuwkoop N.J., van de Kasteele W., Rimmelzwaan G.F., Haanen J.B., Ovaa H., and Schumacher T.N. Design and use of conditional MHC class I ligands. Nat. Med. 12 (2006) 246
37. Weekes M.P., Wills M.R., Mynard K., Carmichael A.J., and Sissons J.G. The memory cytotoxic T-lymphocyte (CTL) response to human cytomegalovirus infection contains individual peptide-specific CTL clones that have undergone extensive expansion in vivo. J. Virol. 73 (1999) 2099
38. Wills M.R., Carmichael A.J., Mynard K., Jin X., Weekes M.P., Plachter B., and Sissons J.G. The human cytotoxic T-lymphocyte (CTL) response to cytomegalovirus is dominated by structural protein pp65: frequency, specificity, and T-cell receptor usage of pp65-specific CTL. J. Virol. 70 (1996) 7569