Engineering yield and rate of reductive biotransformation in Escherichia coli by partial cyclization of the pentose phosphate pathway and PTS-independent glucose transport

Applied Microbiology and Biotechnology

Volumn 93, Issue 4, 2012, Pages 1459-1467

  Siedler, Solvej ^a   Bringer, Stephanie ^a   Blank, Lars M ^b,c   Bott, Michael ^a  

^a FORSCHUNGSZENTRUM JÜLICH   (Germany)

^b TU DORTMUND UNIVERSITY   (Germany)

^c RWTH AACHEN UNIVERSITY   (Germany)

Author keywords

13C flux analysis; Escherichia coli; glf; NADPH yield; pfkA; pfkB; pgi; Reductive whole cell biotransformation

Indexed keywords

FLUX ANALYSIS; GLF; NADPH YIELD; PFKA; PFKB; PGI; WHOLE-CELL BIOTRANSFORMATIONS;

CELL ENGINEERING; ESCHERICHIA COLI; GENES; GLUCOSE; INDUSTRIAL APPLICATIONS; INTERNET PROTOCOLS; OPTIMIZATION; STRAIN;

CYCLIZATION;

6 PHOSPHOFRUCTOKINASE; 6 PHOSPHOFRUCTOKINASE ISOENZYME L; 6 PHOSPHOFRUCTOKINASE ISOENZYME M; ALCOHOL DEHYDROGENASE; BACTERIAL PROTEIN; FRUCTOSE 6 PHOSPHATE; GLUCOKINASE; GLUCOSE; GLUCOSE 6 PHOSPHATE; GLUCOSE 6 PHOSPHATE ISOMERASE; GLUCOSE FACILITATOR; PENTOSE PHOSPHATE; UNCLASSIFIED DRUG;

BIOTRANSFORMATION; CATALYSIS; COLIFORM BACTERIUM; GLUCOSE; METABOLISM; REDOX CONDITIONS;

ARTICLE; BIOCATALYSIS; BIOTRANSFORMATION; CATALYSIS; CYCLIZATION; ESCHERICHIA COLI; GLUCOSE TRANSPORT; METABOLIC ENGINEERING; NONHUMAN; ZYMOMONAS MOBILIS;

ACETOACETATES; BIOTRANSFORMATION; CARBON ISOTOPES; ESCHERICHIA COLI; GENE DELETION; GLUCOSE; HYDROXYBUTYRATES; METABOLIC ENGINEERING; NADP; OXIDATION-REDUCTION; PENTOSE PHOSPHATE PATHWAY; PHOSPHOTRANSFERASES;

ESCHERICHIA COLI; ZYMOMONAS MOBILIS;

EID: 84857912495     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-011-3626-3     Document Type: Article

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