Genotoxicity of pesticide mixtures present in the diet of the French population

Environmental and Molecular Mutagenesis

Volumn 53, Issue 3, 2012, Pages 173-184

  Graillot, Vanessa ^a,b,c   Takakura, Natsuko ^c   Hegarat, Ludovic Le ^c   Fessard, Valerie ^c   Audebert, Marc ^a,b   Cravedi, Jean Pierre ^a,b  

^a TOXALIM RESEARCH CENTRE IN FOOD TOXICOLOGY   (France)

^b UNIVERSITÉ DE TOULOUSE   (France)

^c FRENCH AGENCY FOR FOOD   (France)

Author keywords

H2AX; Genotoxicity; Human cell lines; Mixture of pesticides

Indexed keywords

CASPASE 3; CASPASE 7; CYPRODINIL; FLUDIOXONIL; PESTICIDE; REACTIVE OXYGEN METABOLITE; UNCLASSIFIED DRUG;

ARTICLE; CELL LINE; CELL STRAIN HEPG2; COMET ASSAY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CYTOTOXICITY; DIETARY INTAKE; DNA DAMAGE; ENZYME ACTIVITY; FOOD CONTAMINATION; GENOTOXICITY; HUMAN; HUMAN CELL;

CASPASE 3; CASPASE 7; CELL LINE, TUMOR; COMET ASSAY; COMPLEX MIXTURES; DIET; DNA DAMAGE; DOSE-RESPONSE RELATIONSHIP, DRUG; FOOD CONTAMINATION; FRANCE; HEP G2 CELLS; HUMANS; MUTAGENICITY TESTS; PESTICIDE RESIDUES; REACTIVE OXYGEN SPECIES;

EID: 84857801857     PISSN: 08936692     EISSN: 10982280     Source Type: Journal    
DOI: 10.1002/em.21676     Document Type: Article

References (41)

1. Audebert M, Dolo L, Perdu E, Cravedi JP, Zalko D. 2011. Use of the gammaH2AX assay for assessing the genotoxicity of bisphenol A and bisphenol F in human cell lines. Arch Toxicol 85: 1463-1473.
2. Audebert M, Riu A, Jacques C, Hillenweck A, Jamin EL, Zalko D, Cravedi JP. 2010. Use of the gammaH2AX assay for assessing the genotoxicity of polycyclic aromatic hydrocarbons in human cell lines. Toxicol Lett 199: 182-192.
3. Barnes L, Dumas M, Juan M, Noblesse E, Tesniere A, Schnebert S, Guillot B, Moles JP. 2010. GammaH2AX, an accurate marker that analyzes UV genotoxic effects on human keratinocytes and on human skin. Photochem Photobiol 86: 933-941.
4. Bartkova J, Horejsi Z, Koed K, Kramer A, Tort F, Zieger K, Guldberg P, Sehested M, Nesland JM, Lukas C, Ørntoft T, Lukas J, Bartek J. 2005. DNA damage response as a candidate anti-cancer barrier in early human tumorigenesis. Nature 434: 864-870.
5. Bolognesi C, Creus A, Ostrosky-Wegman P, Marcos R. 2011. Micronuclei and pesticide exposure. Mutagenesis 26: 19-26.
6. Crepet A, Tressou J. 2011. Bayesian nonparametric model for clustering individual co-exposure to pesticides found in the French diet. Bayesian analysis 6: 127-144.
7. Das PP, Shaik AP, Jamil K. 2007. Genotoxicity induced by pesticide mixtures: In-vitro studies on human peripheral blood lymphocytes. Toxicol Ind Health 23: 449-458.
8. EFSA. 2007. Conclusion regarding the peer review of the pesticide risk assessment of the active substance fludioxonil. Available at:.
9. EFSA. 2008. Opinion of the scientific panel on plant protection products and their residues to evaluate the suitability of existing methodologies and, if appropriate, the identification of new approaches to assess cumulative and synergistic risks from pesticides to human health with a view to set MRLs for those pesticides in the frame of Regulation (EC) 396/20051. Available at: 704:1-84.***.
10. EFSA. 2010. Annual Report on Pesticide Residues according to Article 32 of Regulation (EC) n° 396/2005. Available at:.
11. FAO/WHO. 2004. Pesticide residues in food 2004. Available at:.
12. Gorgoulis VG, Vassiliou LV, Karakaidos P, Zacharatos P, Kotsinas A, Liloglou T, Venere M, Ditullio RA, Jr., Kastrinakis NG, Levy B, Kletsas D, Yoneta A, Herlyn M, Kittas C, Halazonetis TD. 2005. Activation of the DNA damage checkpoint and genomic instability in human precancerous lesions. Nature 434: 907-913.
13. Huang R, Southall N, Cho MH, Xia M, Inglese J, Austin CP. 2008. Characterization of diversity in toxicity mechanism using in vitro cytotoxicity assays in quantitative high throughput screening. Chem Res Toxicol 21: 659-667.
14. Isidori M, Caterino E, Criscuolo E, Fatigati V, Liguori G, Parrella A. 2009. Antimutagenic and antigenotoxic effects of vegetable matrices on the activity of pesticides. Food Addit Contam Part A Chem Anal Control Expo Risk Assess 26: 1049-1062.
15. Ismail IH, Wadhra TI, Hammarsten O. 2007. An optimized method for detecting gamma-H2AX in blood cells reveals a significant interindividual variation in the gamma-H2AX response among humans. Nucleic Acids Res 35: e36.
16. Iwanari M, Nakajima M, Kizu R, Hayakawa K, Yokoi T. 2002. Induction of CYP1A1, CYP1A2, and CYP1B1 mRNAs by nitropolycyclic aromatic hydrocarbons in various human tissue-derived cells: Chemical-, cytochrome P450 isoform-, and cell-specific differences. Arch Toxicol 76: 287-298.
17. Khanna KK, Jackson SP. 2001. DNA double-strand breaks: Signaling, repair and the cancer connection. Nat Genet 27: 247-254.
18. Kirkland D, Pfuhler S, Tweats D, Aardema M, Corvi R, Darroudi F, Elhajouji A, Glatt H, Hastwell P, Hayashi M, Kasper P, Kirchner S, Lynch A, Marzin D, Maurici D, Meunier JR, Müller L, Nohynek G, Parry J, Parry E, Thybaud V, Tice R, van Benthem J, Vanparys P, White P. 2007. How to reduce false positive results when undertaking in vitro genotoxicity testing and thus avoid unnecessary follow-up animal tests: Report of an ECVAM Workshop. Mutat Res 628: 31-55.
19. Leopardi P, Cordelli E, Villani P, Cremona TP, Conti L, De Luca G, Crebelli R. 2010. Assessment of in vivo genotoxicity of the rodent carcinogen furan: Evaluation of DNA damage and induction of micronuclei in mouse splenocytes. Mutagenesis 25: 57-62.
20. Levine AJ. 1997. p53, the cellular gatekeeper for growth and division. Cell 88: 323-331.
21. Lioret S, Dubuisson C, Dufour A, Touvier M, Calamassi-Tran G, Maire B, Volatier JL, Lafay L. 2011. Trends in food intake in French children from 1999 to 2007: Results from the INCA (etude Individuelle Nationale des Consommations Alimentaires) dietary surveys. Br J Nutr 103: 585-601.
22. Lodovici M, Casalini C, Briani C, Dolara P. 1997. Oxidative liver DNA damage in rats treated with pesticide mixtures. Toxicology 117: 55-60.
23. Matsuzaki K, Harada A, Takeiri A, Tanaka K, Mishima M. 2010. Whole cell-ELISA to measure the gammaH2AX response of six aneugens and eight DNA-damaging chemicals. Mutat Res 700: 71-79.
24. Muller L, Sofuni T. 2000. Appropriate levels of cytotoxicity for genotoxicity tests using mammalian cells in vitro. Environ Mol Mutagen 35: 202-205.
25. Olgun S, Gogal RM, Jr., Adeshina F, Choudhury H, Misra HP. 2004. Pesticide mixtures potentiate the toxicity in murine thymocytes. Toxicology 196: 181-195.
26. Osseni RA, Debbasch C, Christen MO, Rat P, Warnet JM. 1999. Tacrine-induced reactive oxygen species in a human liver cell line: The role of anethole dithiolethione as a scavenger. Toxicol In Vitro 13: 683-688.
27. Radice S, Fumagalli R, Chiesara E, Ferraris M, Frigerio S, Marabini L. 2004. Estrogenic activity of procymidone in rainbow trout (Oncorhynchus mykiss) hepatocytes: A possible mechanism of action. Chem Biol Interact 147: 185-193.
28. Radice S, Marabini L, Gervasoni M, Ferraris M, Chiesara E. 1998. Adaptation to oxidative stress: Effects of vinclozolin and iprodione on the HepG2 cell line. Toxicology 129: 183-191.
29. Sedelnikova OA, Redon CE, Dickey JS, Nakamura AJ, Georgakilas AG, Bonner WM. 2010. Role of oxidatively induced DNA lesions in human pathogenesis. Mutat Res 704: 152-159.
30. Skipper PL, Kim MY, Sun HL, Wogan GN, Tannenbaum SR. 2010. Monocyclic aromatic amines as potential human carcinogens: Old is new again. Carcinogenesis 31: 50-58.
31. Stuart SA, Wang JY. 2009. Ionizing radiation induces ATM-independent degradation of p21Cip1 in transformed cells. J Biol Chem 284: 15061-15070.
32. Tanaka T, Halicka HD, Huang X, Traganos F, Darzynkiewicz Z. 2006. Constitutive histone H2AX phosphorylation and ATM activation, the reporters of DNA damage by endogenous oxidants. Cell Cycle 5: 1940-1945.
33. Trouiller B, Reliene R, Westbrook A, Solaimani P, Schiestl RH. 2009. Titanium dioxide nanoparticles induce DNA damage and genetic instability in vivo in mice. Cancer Res 69: 8784-8789.
34. Wang Y, Prives C. 1995. Increased and altered DNA binding of human p53 by S and G2/M but not G1 cyclin-dependent kinases. Nature 376: 88-91.
35. Watters GP, Smart DJ, Harvey JS, Austin CA. 2009. H2AX phosphorylation as a genotoxicity endpoint. Mutat Res 679: 50-58.
36. Weichenthal S, Moase C, Chan P. 2010. A review of pesticide exposure and cancer incidence in the Agricultural Health Study cohort. Environ Health Perspect 118: 1117-1125.
37. Wetmore BA, Wambaugh JF, Ferguson SS, Sochaski MA, Rotroff DM, Freeman K, Clewell HJIII, Dix DJ, Andersen ME, Houck KA, Allen B, Judson RS, Singh R, Kavlock RJ, Richard AM, Thomas RS 2011. Integration of dosimetry, exposure and high-throughput screening data in chemical toxicity assessment. Toxicol Sci, doi: 10.1093/toxsci/kfr254.
38. Xia M, Huang R, Witt KL, Southall N, Fostel J, Cho MH, Jadhav A, Smith CS, Inglese J, Portier CJ, Tice RR, Austin CP. 2008. Compound cytotoxicity profiling using quantitative high-throughput screening. Environ Health Perspect 116: 284-291.
39. Yoshikawa T, Kashino G, Ono K, Watanabe M. 2009. Phosphorylated H2AX foci in tumor cells have no correlation with their radiation sensitivities. J Radiat Res (Tokyo) 50: 151-160.
40. Zhang Q, Wang C, Sun L, Li L, Zhao M. 2010. Cytotoxicity of lambda-cyhalothrin on the macrophage cell line RAW 264.7. J Environ Sci (China) 22: 428-432.
41. Zhou C, Li Z, Diao H, Yu Y, Zhu W, Dai Y, Chen FF, Yang J. 2006. DNA damage evaluated by gammaH2AX foci formation by a selective group of chemical/physical stressors. Mutat Res 604: 8-18.