Gene expression of selected signature cytokines of T cell subsets in duodenal tissues of dogs with and without inflammatory bowel disease

Veterinary Immunology and Immunopathology

Volumn 146, Issue 1, 2012, Pages 87-91

  Schmitz, Silke ^a   Garden, Oliver A ^a   Werling, Dirk ^a   Allenspach, Karin ^a  

^a ROYAL VETERINARY COLLEGE   (United Kingdom)

Author keywords

Adaptive immunity; Canine chronic enteropathy; Interleukin 17; Quantitative reverse transcriptase polymerase chain reaction

Indexed keywords

GAMMA INTERFERON; INTERLEUKIN 10; INTERLEUKIN 17; INTERLEUKIN 22; TRANSFORMING GROWTH FACTOR BETA;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DOG; DUODENUM; ENTERITIS; GENE EXPRESSION; LAMINA PROPRIA; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; T LYMPHOCYTE; T LYMPHOCYTE SUBPOPULATION; TH17 CELL; UPREGULATION;

ANIMALS; CYTOKINES; DOG DISEASES; DOGS; DUODENUM; FEMALE; INFLAMMATORY BOWEL DISEASES; MALE; RNA, MESSENGER; T-LYMPHOCYTE SUBSETS;

CANIS FAMILIARIS;

EID: 84857796312     PISSN: 01652427     EISSN: 18732534     Source Type: Journal    
DOI: 10.1016/j.vetimm.2012.01.013     Document Type: Article

References (25)

1. Brand S., Beigel F., Olszak T., Zitzmann K., Eichhorst S.T., Otte J.M., Diepolder H., Marquardt A., Jagla W., Popp A., Leclair S., Herrmann K., Seiderer J., Ochsenkuhn T., Goke B., Auernhammer C.J., Dambacher J. IL-22 is increased in active Crohn's disease and promotes proinflammatory gene expression and intestinal epithelial cell migration. Am. J. Physiol. Gastrointest. Liver Physiol. 2006, 290:G827-G838.
2. Cua D.J., Tato C.M. Innate IL-17-producing cells: the sentinels of the immune system. Nat. Rev. Immunol. 2010, 10:479-489.
3. De Majo M., Pugliese M., Galia S., Mazzullo G., La Camera E., Fera M.T. Cytokine mRNA quantification in gastro-intestinal biopsies of dogs with idiopathic chronic enteropathies by Real Time RT-PCR: preliminary results. Vet. Res. Commun. 2008, 32(Suppl 1):S275-S277.
4. Fujino S., Andoh A., Bamba S., Ogawa A., Hata K., Araki Y., Bamba T., Fujiyama Y. Increased expression of interleukin 17 in inflammatory bowel disease. Gut 2003, 52:65-70.
5. Funke B. Laser microdissection of intestinal epithelial cells and downstream analysis. Methods Mol. Biol. 2011, 755:189-196.
6. German A.J., Helps C.R., Hall E.J., Day M.J. Cytokine mRNA expression in mucosal biopsies from German shepherd dogs with small intestinal enteropathies. Dig. Dis. Sci. 2000, 45:7-17.
7. Ivanov I.I., McKenzie B.S., Zhou L., Tadokoro C.E., Lepelley A., Lafaille J.J., Cua D.J., Littman D.R. The orphan nuclear receptor RORgammat directs the differentiation program of proinflammatory IL-17+ T helper cells. Cell 2006, 126:1121-1133.
8. Ivanov I.I., Zhou L., Littman D.R. Transcriptional regulation of Th17 cell differentiation. Semin. Immunol. 2007, 19:409-417.
9. Kinugasa T., Sakaguchi T., Gu X., Reinecker H.C. Claudins regulate the intestinal barrier in response to immune mediators. Gastroenterology 2000, 118:1001-1011.
10. Lee Y.K., Turner H., Maynard C.L., Oliver J.R., Chen D., Elson C.O., Weaver C.T. Late developmental plasticity in the T helper 17 lineage. Immunity 2009, 30:92-107.
11. Maccoux L.J., Clements D.N., Salway F., Day P.J. Identification of new reference genes for the normalisation of canine osteoarthritic joint tissue transcripts from microarray data. BMC Mol. Biol. 2007, 8:62.
12. Maul J., Loddenkemper C., Mundt P., Berg E., Giese T., Stallmach A., Zeitz M., Duchmann R. Peripheral and intestinal regulatory CD4+ CD25(high) T cells in inflammatory bowel disease. Gastroenterology 2005, 128:1868-1878.
13. McMahon L.A., House A.K., Catchpole B., Elson-Riggins J., Riddle A., Smith K., Werling D., Burgener I.A., Allenspach K. Expression of Toll-like receptor 2 in duodenal biopsies from dogs with inflammatory bowel disease is associated with severity of disease. Vet. Immunol. Immunopathol. 2010, 135:158-163.
14. Melgar S., Yeung M.M., Bas A., Forsberg G., Suhr O., Oberg A., Hammarstrom S., Danielsson A., Hammarstrom M.L. Over-expression of interleukin 10 in mucosal T cells of patients with active ulcerative colitis. Clin. Exp. Immunol. 2003, 134:127-137.
15. O'Connor W., Kamanaka M., Booth C.J., Town T., Nakae S., Iwakura Y., Kolls J.K., Flavell R.A. A protective function for interleukin 17A in T cell-mediated intestinal inflammation. Nat. Immunol. 2009, 10:603-609.
16. Peters I.R., Peeters D., Helps C.R., Day M.J. Development and application of multiple internal reference (housekeeper) gene assays for accurate normalisation of canine gene expression studies. Vet. Immunol. Immunopathol. 2007, 117:55-66.
17. Pinheiro D., Singh Y., Grant C.R., Appleton R.C., Sacchini F., Walker K.R., Chadbourne A.H., Palmer C.A., Armitage-Chan E., Thompson I., Williamson L., Cunningham F., Garden O.A. Phenotypic and functional characterization of a CD4(+) CD25(high) FOXP3(high) regulatory T-cell population in the dog. Immunology 2011, 132:111-122.
18. Rozen S., Skaletsky H. Primer3 on the WWW for general users and for biologist programmers. Methods Mol. Biol. 2000, 132:365-386.
19. Sartor R.B. Mechanisms of disease: pathogenesis of Crohn's disease and ulcerative colitis. Nat. Clin. Pract. Gastroenterol. Hepatol. 2006, 3:390-407.
20. Schreiber S., Heinig T., Thiele H.G., Raedler A. Immunoregulatory role of interleukin 10 in patients with inflammatory bowel disease. Gastroenterology 1995, 108:1434-1444.
21. Shen W., Durum S.K. Synergy of IL-23 and Th17 cytokines: new light on inflammatory bowel disease. Neurochem. Res. 2010, 35:940-946.
22. Sugimoto K., Ogawa A., Mizoguchi E., Shimomura Y., Andoh A., Bhan A.K., Blumberg R.S., Xavier R.J., Mizoguchi A. IL-22 ameliorates intestinal inflammation in a mouse model of ulcerative colitis. J. Clin. Invest. 2008, 118:534-544.
23. Vandesompele J., De Preter K., Pattyn F., Poppe B., Van Roy N., De Paepe A., Speleman F. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 2002, 3. RESEARCH0034.
24. Veldhoen M., Hirota K., Westendorf A.M., Buer J., Dumoutier L., Renauld J.C., Stockinger B. The aryl hydrocarbon receptor links TH17-cell-mediated autoimmunity to environmental toxins. Nature 2008, 453:106-109.
25. Zheng Y., Valdez P.A., Danilenko D.M., Hu Y., Sa S.M., Gong Q., Abbas A.R., Modrusan Z., Ghilardi N., de Sauvage F.J., Ouyang W. Interleukin-22 mediates early host defense against attaching and effacing bacterial pathogens. Nat. Med. 2008, 14:282-289.