Cytokine mRNA quantification in gastro-intestinal biopsies of dogs with idiopathic chronic enteropathies by Real Time RT-PCR: Preliminary results

Veterinary Research Communications

Volumn 32, Issue SUPPL. 1, 2008, Pages

  De Majo, M ^a,b   Pugliese, M ^a   Galia, S ^a   Mazzullo, G ^a   La Camera, E ^a   Fera, M T ^a  

^a UNIVERSITY OF MESSINA   (Italy)

^b Polo Universitario Annunziata   (Italy)

Author keywords

Cytokine; Dog; Inflammatory bowel disease; Intestine

Indexed keywords

CYTOKINE; GAMMA INTERFERON; INTERLEUKIN 4; MESSENGER RNA; TUMOR NECROSIS FACTOR ALPHA;

ANTIBIOTIC THERAPY; BLOOD EXAMINATION; CHRONIC DISEASE; CLINICAL EXAMINATION; COLONOSCOPY; CONFERENCE PAPER; DEFECATION DISORDER; DIARRHEA; DISEASE DURATION; DOG; ENTERITIS; ENTEROPATHY; GASTROINTESTINAL BIOPSY; GASTROINTESTINAL ENDOSCOPY; GENE EXPRESSION; HEMATOCHEZIA; HISTOPATHOLOGY; INTESTINE BIOPSY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; STOMACH BIOPSY; TENESMUS; VOMITING; WEIGHT REDUCTION;

ANIMALS; BIOPSY; CD4-POSITIVE T-LYMPHOCYTES; CYTOKINES; DOG DISEASES; DOGS; FEMALE; INFLAMMATORY BOWEL DISEASES; INTERFERON-GAMMA; INTERLEUKIN-4; MALE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; TUMOR NECROSIS FACTOR-ALPHA;

CANIS FAMILIARIS;

EID: 52049092379     PISSN: 01657380     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11259-008-9127-x     Document Type: Conference Paper

References (4)

1. Bustin S.A., 2002. Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): Trends and problems. Journal of Molecular Endocrinology, 29, 23-39.
2. Fujiwara S., Yasanuga S., Nakayama H., Doi K., Masuda K., Ohno K., Tsujimoto H., 2002. Quantitative analysis of cytokines messenger RNAs in the duodenal mucosa in dogs with small intestinal inflammatory bowel disease. Journal of Veterinary Internal Medicine, 16, 327-336.
3. Peters I.R., Helps C.R., Calvert E.L., Hal E.J., Day M.J., 2005. Cytokine mRNA quantification in duodenal mucosa from dogs with chronic enteropathies by Real-Time Reverse Transcriptase Polymerase Chain Reaction. Journal of Veterinary Internal Medicine, 19, 644-653.
4. Ridyard A.E., Nuttall T.J., Else R.W., Simpson J.W., Miller H.R., 2002.Evaluation of Th1, Th2 and immunosuppressive cytokine mRNA expression within the colonic mucosa of dogs with idiopathic lymphocytic-plasmacytic colitis. Veterinary Immunology and Immunopathology, 86, 205-214.