GEF-H1-RhoA signaling pathway mediates LPS-induced NF-κB transactivation and IL-8 synthesis in endothelial cells

Molecular Immunology

Volumn 50, Issue 1-2, 2012, Pages 98-107

  Guo, Feng ^a   Tang, Jiajun ^a   Zhou, Zengding ^a   Dou, Yi ^a   Van Lonkhuyzen, Derek ^b   Gao, Chengjin ^c   Huan, Jingning ^a  

^a SHANGHAI SECOND MEDICAL UNIVERSITY   (China)

^b QUEENSLAND UNIVERSITY OF TECHNOLOGY   (Australia)

^c TENTH PEOPLE S HOSPITAL OF TONGJI UNIVERSITY   (China)

Author keywords

Guanine nucleotide exchange factor; Interleukin 8; Lipopolysaccharide; Nuclear factor B; P38; RhoA

Indexed keywords

CLOSTRIDIUM DIFFICILE TOXIN B; GUANINE NUCLEOTIDE EXCHANGE FACTOR; GUANINE NUCLEOTIDE EXCHANGE FACTOR H1; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; INTERLEUKIN 8; LIPOPOLYSACCHARIDE; MITOGEN ACTIVATED PROTEIN KINASE P38; MYELOID DIFFERENTIATION FACTOR 88; RHOA GUANINE NUCLEOTIDE BINDING PROTEIN; TOLL LIKE RECEPTOR 4; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; CYTOKINE RELEASE; ENDOTHELIUM CELL; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; SIGNAL TRANSDUCTION; UPREGULATION;

BACTERIAL PROTEINS; BACTERIAL TOXINS; BLOTTING, WESTERN; CELLS, CULTURED; DOSE-RESPONSE RELATIONSHIP, DRUG; GENE EXPRESSION REGULATION; GUANINE NUCLEOTIDE EXCHANGE FACTORS; HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS; HUMANS; INTERLEUKIN-8; LIPOPOLYSACCHARIDES; MITOGEN-ACTIVATED PROTEIN KINASES; MYELOID DIFFERENTIATION FACTOR 88; NF-KAPPA B; PHOSPHORYLATION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RHOA GTP-BINDING PROTEIN; RNA INTERFERENCE; SIGNAL TRANSDUCTION; TIME FACTORS; TOLL-LIKE RECEPTOR 4; TRANSCRIPTIONAL ACTIVATION; UP-REGULATION;

CLOSTRIDIUM DIFFICILE;

EID: 84857036975     PISSN: 01615890     EISSN: 18729142     Source Type: Journal    
DOI: 10.1016/j.molimm.2011.12.009     Document Type: Article

References (37)

1. Aird W.C. The role of the endothelium in severe sepsis and multiple organ dysfunction syndrome. Blood 2003, 101:3765-3777.
2. Ait-Oufella H., Maury E., Lehoux S., Guidet B., Offenstadt G. The endothelium: physiological functions and role in microcirculatory failure during severe sepsis. Intensive Care Med. 2010, 36:1286-1298.
3. Akira S., Takeda K. Toll-like receptor signalling. Nat. Rev. Immunol. 2004, 4:499-511.
4. Baggiolini M., Dewald B., Moser B. Human chemokines: an update. Annu. Rev. Immunol. 1997, 15:675-705.
5. Birkenfeld J., Nalbant P., Bohl B.P., Pertz O., Hahn K.M., Bokoch G.M. GEF-H1 modulates localized RhoA activation during cytokinesis under the control of mitotic kinases. Dev. Cell 2007, 12:699-712.
6. Cook-Mills J.M., Deem T.L. Active participation of endothelial cells in inflammation. J. Leukoc. Biol. 2005, 77:487-495.
7. Doe C., Bentley R., Behm D.J., Lafferty R., Stavenger R., Jung D., Bamford M., Panchal T., Grygielko E., Wright L.L., Smith G.K., Chen Z., Webb C., Khandekar S., Yi T., Kirkpatrick R., Dul E., Jolivette L., Marino J.P., Willette R., Lee D., Hu E. Novel Rho kinase inhibitors with anti-inflammatory and vasodilatory activities. J. Pharmacol. Exp. Ther. 2007, 320:89-98.
8. Fiorentini C., Falzano L., Travaglione S., Fabbri A. Hijacking Rho GTPases by protein toxins and apoptosis: molecular strategies of pathogenic bacteria. Cell Death Differ. 2003, 10:147-152.
9. Goebeler M., Gillitzer R., Kilian K., Utzel K., Bröcker E.B., Rapp U.R., Ludwig S. Multiple signaling pathways regulate NF-kappaB-dependent transcription of the monocyte chemoatt Hijacking Rho GTPases by protein toxins and apoptosis: molecular strategies of pathogenic bacteria ractant protein-1 gene in primary endothelial cells. Blood 2001, 97:46-55.
10. Hall A. Small GTP-binding proteins and the regulation of the actin cytoskeleton. Annu. Rev. Cell Biol. 1994, 10:31-54.
11. Hall A., Etienne-Manneville S. Rho GTPases in cell biology. Nature 2002, 420:629-635.
12. Hippenstiel S., Soeth S., Kellas B., Fuhrmann O., Seybold J., Krüll M., Eichel-Streiber C., Goebeler M., Ludwig S., Suttorp N. Rho proteins and the p38-MAPK pathway are important mediators for LPS-induced interleukin-8 expression in human endothelial cells. Blood 2000, 95:3044-3051.
13. Isowa N., Keshavjee S.H., Liu M. Role of microtubules in LPS-induced macrophage inflammatory protein-2 production from rat pneumocytes. Am. J. Physiol. Lung Cell. Mol. Physiol. 2000, 279:L1075-L1082.
14. Kawai T., Adachi O., Ogawa T., Takeda K., Akira S. Unresponsiveness of MyD88-deficient mice to endotoxin. Immunity 1999, 11:115-122.
15. Kim J.H., Woo C.H. Rac GTPase activity is essential for lipopolysaccharide signaling to extracellular signal-regulated kinase and p38 MAP kinase activation in rat-2 fibroblasts. Mol. Cells 2002, 13:470-475.
16. Kong L., Ge B.X. MyD88-independent activation of a novel actin-Cdc42/Rac pathway is required for Toll-like receptor-stimulated phagocytosis. Cell Res. 2008, 18:745-755.
17. Krendel M., Zenke F.T., Bokoch G.M. Nucleotide exchange factor GEF-H1 mediates cross-talk between microtubules and the actin cytoskeleton. Nature Cell Human chemokines: an update. Annu. Rev. Immunol. Biol. 2002, 4:294-301.
18. Liu-Bryan R., Pritzker K., Firestein G.S., Terkeltaub R. TLR2 signaling in chondrocytes drives calcium pyrophosphate dihydrate and monosodium urate crystal-induced nitric oxide generation. J. Immunol. 2005, 174:5016-5023.
19. Mukaida N., Matsumoto T., Yokoi K., Harada A., Matsushima K. Inhibition of neutrophil-mediated acute inflammation injury by an antibody against interleukin-8 (IL-8). Inflamm. Res. 1998, 47:S151-S157.
20. Muñoz C., Pascual-Salcedo D., Castellanos M.C., Alfranca A., Aragonés J., Vara A., Redondo J.M., de Landázuri M.O. Pyrrolidine dithiocarbamate inhibits the production of interleukin-6, interleukin-8, and granulocyte-macrophage colony-stimulating factor by human endothelial cells in response to inflammatory mediators: modulation of NF-kappa B and AP-1 transcription factors activity. Blood 1996, 88(9):3482-3490.
21. Perona R., Montaner S., Saniger L., Sánchez-Pérez I., Bravo R., Lacal J.C. Activation of the nuclear factor-kappaB by Rho, CDC42, and Rac-1 proteins. Genes Dev. 1997, 11:463-475.
22. Rajan S., Ye J., Bai S., Huang F., Guo Y.L. NF-kappaB, but not p38 MAP kinase, is required for TNF-alpha-induced expression of cell adhesion molecules in endothelial cells. J. Cell. Biochem. 2008, 105:477-486.
23. Ren X.D., Schwartz M.A. Determination of GTP loading on Rho. Methods Enzymol. 2000, 325:264-272.
24. Ren Y., Li R., Zheng Y., Busch H. Cloning and characterization of GEF-H1, a microtubule-associated guanine nucleotide exchange factor for Rac and Rho GTPases. J. Biol. Chem. 1998, 273:34954-34960.
25. Riento K., Ridley A.J. Rocks: multifunctional kinases in cell behaviour. Nat. Rev. Mol. Cell Biol. 2003, 4:446-456.
26. Ruse M., Knaus U.G. New players in TLR-mediated innate immunity: PI3 K and small Rho GTPases. Immunol. Res. 2006, 34:33-48.
27. Saha R.N., Jana M., Pahan K. MAPK p38 regulates transcriptional activity of NF-kappaB in primary human astrocytes via acetylation of p65. J. Immunol. 2007, 179:7101-7109.
28. Saha R.N., Jana M., Pahan K. The p38 mitogen-activated protein kinase regulates interleukin-1 beta-induced IL-8 expression via an effect on the IL-8 promoter in intestinal epithelial cells. Immunology 2003, 108:502-512.
29. Shibolet O., Giallourakis C., Rosenberg I., Mueller T., Xavier R.J., Podolsky D.K. AKAP13, a RhoA GTPase-specific guanine exchange factor, is a novel regulator of TLR2 signaling. J. Biol. Chem. 2007, 282:35308-35317.
30. Smart S.J., Casale T.B. Interleukin-8-induced transcellular neutrophil migration is facilitated by endothelial and pulmonary epithelial cells. Am. J. Respir. Cell Mol. Biol. 1993, 9:489-495.
31. Stryjewski G.R., Nylen E.S., Bell M.J., Snider R.H., Becker K.L., Wu A., Lawlor C., Dalton H. Interleukin-6, interleukin-8, and a rapid and sensitive assay for calcitonin precursors for the determination of bacterial sepsis in febrile neutropenic children. Pediatr. Crit. Care Med. 2005, 6:129-135.
32. Vojtek A.B., Cooper J.A. Rho family members: activators of MAP kinase cascades. Cell 1995, 82:527-529.
33. Weighardt H., Holzmann B. Role of Toll-like receptor responses for sepsis pathogenesis. Immunobiology 2007, 212:715-722.
34. Wendel M., Paul R., Heller A.R. Lipoproteins in inflammation and sepsis. II. Clinical aspects. Intensive Care Med. 2007, 33:25-35.
35. Wertheim W.A., Kunkel S.L., Standiford T.J., Burdick M.D., Becker F.S., Wilke C.A., Gilbert A.R., Strieter R.M. Regulation of neutrophil-derived IL-8: the role of prostaglandin E2, dexamethasone, and IL-4. J. Immunol. 1993, 151:2166-2175.
36. Yasumoto K., Okamoto S., Mukaida N., Murakami S., Mai M., Matsushima K. Tumor necrosis factor alpha and interferon gamma synergistically induce interleukin 8 production in a human gastric cancer cell line through acting concurrently on AP-1 and NF-kB-like binding sites of the interleukin 8 gene. J. Biol. Chem. 1992, 267:22506-32251.
37. Zheng Y. Dbl family guanine nucleotide exchange factors. Trends Biochem. Sci. 2001, 26:724-732.