Proteomics analyses of human optic nerve head astrocytes following biomechanical strain

Molecular and Cellular Proteomics

Volumn 11, Issue 2, 2012, Pages

  Rogers, Ronan S ^a,b,c   Dharsee, Moyez ^d   Ackloo, Suzanne ^d   Sivak, Jeremy M ^b,c   Flanagan, John G ^a,b,c,e  

^a UNIVERSITY OF TORONTO   (Canada)

^b UNIVERSITY HEALTH NETWORK   (Canada)

^c TORONTO WESTERN HOSPITAL   (Canada)

^d Ontario Cancer Biomarker Network   (Canada)

^e UNIVERSITY OF WATERLOO   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

CASPASE 3; PROTEIN P53; TRANSFORMING GROWTH FACTOR BETA1; TUMOR NECROSIS FACTOR; PROTEOME;

ARTICLE; BIOMECHANICS; CELL LABELING; CONTROLLED STUDY; GLAUCOMA; GLIA CELL; HUMAN; HUMAN CELL; INTRAOCULAR HYPERTENSION; LIQUID CHROMATOGRAPHY; NERVE CELL CULTURE; NORMAL HUMAN; NUCLEOTIDE SEQUENCE; OPTIC NERVE; OPTIC NERVE DISEASE; PRIORITY JOURNAL; PROTEIN ISOLATION; PROTEOMICS; TANDEM MASS SPECTROMETRY; ASTROCYTE; CELL CULTURE; CYTOLOGY; ENZYME IMMUNOASSAY; GLIA; MECHANICAL STRESS; METABOLISM; OPTIC DISK; PATHOLOGY; WESTERN BLOTTING;

ASTROCYTES; BLOTTING, WESTERN; CELLS, CULTURED; CHROMATOGRAPHY, LIQUID; HUMANS; IMMUNOENZYME TECHNIQUES; NEUROGLIA; OPTIC DISK; OPTIC NERVE DISEASES; PROTEOME; PROTEOMICS; STRESS, MECHANICAL; TANDEM MASS SPECTROMETRY;

EID: 84856689509     PISSN: 15359476     EISSN: 15359484     Source Type: Journal    
DOI: 10.1074/mcp.M111.012302     Document Type: Article

References (105)

1. Quigley, H. A., and Broman, A. T. (2006) The number of people with glaucoma worldwide in 2010 and 2020. Br. J. Ophthalmol. 90, 262-267
2. Krupin, T., Ritch, R., and Shields, M. B. (1996) The glaucomas. 2nd ed., St. Louis: Mosby. 3 v. (xxvi, 1807, 27 p.).
3. Quigley, H. A., (2005) New paradigms in the mechanisms and management of glaucoma. Eye 19, 1241-1248
4. Leske, M. C., Heijl, A., Hussein, M., Bengtsson, B., Hyman, L., and Komaroff, E. (2003) Factors for glaucoma progression and the effect of treatment: the early manifest glaucoma trial. Arch. Ophthalmol. 121, 48-56 (Pubitemid 36401765)
5. Heijl, A., Leske, M. C., Bengtsson, B., Hyman, L., Bengtsson, B., and Hussein, M. (2002) Reduction of intraocular pressure and glaucoma progression: results from the Early Manifest Glaucoma Trial. Arch. Ophthalmol. 120, 1268-1279 (Pubitemid 36105431)
6. Anderson, D. R., Drance, S. M., and Schulzer, M. (2001) Natural history of normal-tension glaucoma. Ophthalmology 108, 247-253 (Pubitemid 32115951)
7. Nickells, R. W. (2007) Ganglion cell death in glaucoma: from mice to men. Vet. Ophthalmol. 10 Suppl 1, 88-94
8. Hernandez, M. R., Miao, H., and Lukas, T. (2008) Astrocytes in glaucomatous optic neuropathy. Prog. Brain Res. 173, 353-373
9. Liu, B., Chen, H., Johns, T. G., and Neufeld, A. H. (2006) Epidermal growth factor receptor activation: an upstream signal for transition of quiescent astrocytes into reactive astrocytes after neural injury. J. Neurosci. 26, 7532-7540 (Pubitemid 44315222)
10. Wyss-Coray, T., and Mucke, L. (2002) Inflammation in neurodegenerative disease-a double-edged sword. Neuron 35, 419-432 (Pubitemid 34879943)
11. Tacconi, M. T. (1998) Neuronal death: is there a role for astrocytes? Neurochem. Res. 23, 759-765
12. Mrak, R. E., and Griffinbc, W. S. (2001) The role of activated astrocytes and of the neurotrophic cytokine S100B in the pathogenesis of Alzheimer's disease. Neurobiol/Aging 22, 915-922 (Pubitemid 34066559)
13. Duncan, A. J., and Heales, S. J. (2005) Nitric oxide and neurological disorders. Mol. Aspects Med. 26, 67-96 (Pubitemid 40250165)
14. Tezel, G. (2010) The immune response in glaucoma: a perspective on the roles of oxidative stress. Exp. Eye Res. 93(2), 178-186
15. Tezel, G. (2008) TNF-alpha signaling in glaucomatous neurodegeneration. Prog. Brain Res. 173, 409-421
16. Tezel, G., Yang, X., Luo, C., Cai, J., Kain, A. D., Powell, D. W., Kuehn, M. H., and Pierce, W. M. (2010) Hemoglobin expression and regulation in glaucoma: insights into retinal ganglion cell oxygenation. Invest. Ophthalmol. Vis. Sci. 51, 907-919
17. Tezel, G. (2009) The role of glia, mitochondria, and the immune system in glaucoma. Invest. Ophthalmol. Vis. Sci. 50, 1001-1012
18. Attwell, D., Buchan, A. M., Charpak, S., Lauritzen, M., Macvicar, B. A., and Newman, E. A. (2010) Glial and neuronal control of brain blood flow. Nature 468, 232-243
19. Eroglu, C., and Barres, B. A. (2010) Regulation of synaptic connectivity by glia. Nature 468, 223-231
20. Nave, K. A. (2010) Myelination and support of axonal integrity by glia. Nature 468, 244-252
21. Ransohoff, R. M., and Cardona, A. E. (2010) The myeloid cells of the central nervous system parenchyma. Nature 468, 253-262
22. Rowitch, D. H., and Kriegstein, A. R. (2010) Developmental genetics of vertebrate glial-cell specification. Nature 468, 214-222
23. Yang, P., and Hernandez, M. R. (2003) Purification of astrocytes from adult human optic nerve heads by immunopanning. Brain Res. Brain Res. Protoc. 12, 67-76 (Pubitemid 37410234)
24. Hernandez, M. R., Agapova, O. A., Yang, P., Salvador-Silva, M., Ricard, C. S., and Aoi, S. (2002) Differential gene expression in astrocytes from human normal and glaucomatous optic nerve head analyzed by cDNA microarray. Glia 38, 45-64
25. Sigal, I. A., Flanagan, J. G., Tertinegg, I., and Ethier, C. R. (2004) Finite element modeling of optic nerve head biomechanics. Invest. Ophthalmol. Vis. Sci. 45, 4378-4387 (Pubitemid 39564663)
26. Sigal, I. A., Flanagan, J. G., and Ethier, C. R. (2005) Factors influencing optic nerve head biomechanics. Invest. Ophthalmol. Vis. Sci. 46, 4189-4199 (Pubitemid 44264622)
27. Sigal, I. A., Flanagan, J. G., Tertinegg, I., and Ethier, C. R. (2005) Reconstruction of human optic nerve heads for finite element modeling. Technol. Health Care 13, 313-329 (Pubitemid 41132442)
28. Sigal, I. A., Flanagan, J. G., Tertinegg, I., and Ethier, C. R. (2009) Modeling individual-specific human optic nerve head biomechanics. Part I: IOPinduced deformations and influence of geometry. Biomech. Model Mechanobiol. 8, 85-98
29. Sigal, I. A., Flanagan, J. G., Tertinegg, I., and Ethier, C. R. (2009) Modeling individual-specific human optic nerve head biomechanics. Part II: influence of material properties. Biomech. Model Mechanobiol. 8, 99-109
30. Norman, R. E., Flanagan, J. G., Rausch, S. M., Sigal, I. A., Tertinegg, I., Eilaghi, A., Portnoy, S., Sled, J. G., and Ethier, C. R. (2010) Dimensions of the human sclera: Thickness measurement and regional changes with axial length. Exp. Eye Res. 90, 277-284
31. Sigal, I. A., Flanagan, J. G., Tertinegg, I., and Ethier, C. R. (2010) 3D morphometry of the human optic nerve head. Exp. Eye Res. 90, 70-80
32. Norman, R. E., Flanagan, J. G., Sigal, I. A., Rausch, S. M., Tertinegg, I., and Ethier, C. R. (2011) Finite element modeling of the human sclera: Influence on optic nerve head biomechanics and connections with glaucoma. Exp. Eye Res. 93(1), 4-12
33. Eilaghi, A., Flanagan, J. G., Simmons, C. A., and Ethier, C. R. (2010) Effects of scleral stiffness properties on optic nerve head biomechanics. Ann. Biomed. Eng. 38, 1586-1592
34. Eilaghi, A., Flanagan, J. G., Tertinegg, I., Simmons, C. A., Wayne Brodland, G., and Ross Ethier, C. (2010) Biaxial mechanical testing of human sclera. J. Biomech. 43, 1696-1701
35. Sigal, I. A., Flanagan, J. G., Tertinegg, I., and Ethier, C. R. (2007) Predicted extension, compression and shearing of optic nerve head tissues. Exp. Eye Res. 85, 312-322 (Pubitemid 47259048)
36. Ethier, C. R. (2006) Scleral biomechanics and glaucoma-a connection? Can. J. Ophthalmol. 41, 9-12, 14
37. Bellezza, A. J., Hart, R. T., and Burgoyne, C. F. (2000) The optic nerve head as a biomechanical structure: initial finite element modeling. Invest. Ophthalmol. Vis. Sci. 41, 2991-3000
38. Burgoyne, C. F., Downs, J. C., Bellezza, A. J., Suh, J. K., and Hart, R. T. (2005) The optic nerve head as a biomechanical structure: a new paradigm for understanding the role of IOP-related stress and strain in the pathophysiology of glaucomatous optic nerve head damage. Prog. Retin. Eye Res. 24, 39-73 (Pubitemid 39535155)
39. Downs, J. C., Suh, J. K., Thomas, K. A., Bellezza, A. J., Burgoyne, C. F., and Hart, R. T. (2003) Viscoelastic characterization of peripapillary sclera: material properties by quadrant in rabbit and monkey eyes. J. Biomech. Eng. 125, 124-131 (Pubitemid 36297235)
40. Downs, J. C., Suh, J. K., Thomas, K. A., Bellezza, A. J., Hart, R. T., and Burgoyne, C. F. (2005) Viscoelastic material properties of the peripapillary sclera in normal and early-glaucoma monkey eyes. Invest. Ophthalmol. Vis. Sci. 46, 540-546 (Pubitemid 40270333)
41. Yang, H., Downs, J. C., Girkin, C., Sakata, L., Bellezza, A., Thompson, H., and Burgoyne, C. F. (2007) 3-D histomorphometry of the normal and early glaucomatous monkey optic nerve head: lamina cribrosa and peripapillary scleral position and thickness. Invest. Ophthalmol. Vis. Sci. 48, 4597-4607 (Pubitemid 351260977)
42. Downs, J. C., Yang, H., Girkin, C., Sakata, L., Bellezza, A., Thompson, H., and Burgoyne, C. F. (2007) Three-dimensional histomorphometry of the normal and early glaucomatous monkey optic nerve head: neural canal and subarachnoid space architecture. Invest. Ophthalmol. Vis. Sci. 48, 3195-3208 (Pubitemid 351261164)
43. Yang, H., Downs, J. C., Bellezza, A., Thompson, H., and Burgoyne, C. F. (2007) 3-D histomorphometry of the normal and early glaucomatous monkey optic nerve head: prelaminar neural tissues and cupping. Invest. Ophthalmol. Vis. Sci. 48, 5068-5084 (Pubitemid 351260917)
44. Sigal, I. A., Yang, H., Roberts, M. D., and Downs, J. C. (2010) Morphing methods to parameterize specimen-specific finite element model geometries. J. Biomech. 43, 254-262
45. Hernandez, M. R., and Ye, H. (1993) Glaucoma: changes in extracellular matrix in the optic nerve head. Ann. Med. 25, 309-315 (Pubitemid 23250266)
46. Varela, H. J., and Hernandez, M. R. (1997) Astrocyte responses in human optic nerve head with primary open-angle glaucoma. J. Glaucoma. 6, 303-313 (Pubitemid 28050922)
47. Kirwan, R. P., Crean, J. K., Fenerty, C. H., Clark, A. F., and O'Brien, C. J. (2004) Effect of cyclical mechanical stretch and exogenous transforming growth factor-beta1 on matrix metalloproteinase-2 activity in lamina cribrosa cells from the human optic nerve head. J. Glaucoma 13, 327-334 (Pubitemid 38944202)
48. Kirwan, R. P., Fenerty, C. H., Crean, J., Wordinger, R. J., Clark, A. F., and O'Brien, C. J. (2005) Influence of cyclical mechanical strain on extracellular matrix gene expression in human lamina cribrosa cells in vitro. Mol. Vis. 11, 798-810 (Pubitemid 41410191)
49. Quill, B., Docherty, N. G., Clark, A. F., and O'Brien, C. J. (2011) The effect of graded cyclic stretching on extracellular matrix-related gene expression profiles in cultured primary human lamina cribrosa cells. Invest. Ophthalmol. Vis. Sci. 52, 1908-1915,
50. Hernandez, M. R. (2000) The optic nerve head in glaucoma: role of astrocytes in tissue remodeling. Prog. Retin. Eye Res. 19, 297-321
51. Hernandez, M. R., Andrzejewska, W. M., and Neufeld, A. H. (1990) Changes in the extracellular matrix of the human optic nerve head in primary open-angle glaucoma. Am. J. Ophthalmol. 109, 180-188 (Pubitemid 20048416)
52. Hernandez, M. R., Ye, H., and Roy, S. (1994) Collagen type IV gene expression in human optic nerve heads with primary open angle glaucoma. Exp. Eye Res. 59, 41-51 (Pubitemid 24257143)
53. Bhattacharya, S. K., Crabb, J. S., Bonilha, V. L., Gu, X., Takahara, H., and Crabb, J. W. (2006) Proteomics implicates peptidyl arginine deiminase 2 and optic nerve citrullination in glaucoma pathogenesis. Invest. Ophthalmol. Vis. Sci. 47, 2508-2514 (Pubitemid 351639834)
54. Lei, Y., Rajabi, S., Pedrigi, R. M., Overby, D. R., Read, A. T., and Ethier, C. R. (2011) In vitro models for glaucoma research: effects of hydrostatic pressure. Invest. Ophthalmol. Vis. Sci. 52, 6329-6339
55. Steely, H. T., Jr., and Clark, A. F. (2000) The use of proteomics in ophthalmic research. Pharmacogenomics 1, 267-280
56. Steely, H. T., Dillow, G. W., Bian, L., Grundstad, J., Braun, T. A., Casavant, T. L., McCartney, M. D., and Clark, A. F. (2006) Protein expression in a transformed trabecular meshwork cell line: proteome analysis. Mol. Vis. 12, 372-383
57. Zhao, X., Ramsey, K. E., Stephan, D. A., and Russell, P. (2004) Gene and protein expression changes in human trabecular meshwork cells treated with transforming growth factor-beta. Invest. Ophthalmol. Vis. Sci. 45, 4023-4034 (Pubitemid 39411157)
58. Fuchshofer, R. (2011) The pathogenic role of transforming growth factorbeta2 in glaucomatous damage to the optic nerve head. Exp. Eye Res. 93, 165-169
59. Bhattacharya, S. K. (2009) Retinal deimination in aging and disease. IUBMB Life 61, 504-509
60. Govindarajan, B., Junk, A., Algeciras, M., Salomon, R. G., and Bhattacharya, S. K. (2009) Increased isolevuglandin-modified proteins in glaucomatous astrocytes. Mol. Vis. 15, 1079-1091
61. Tezel, G., Yang, X., and Cai, J. (2005) Proteomic identification of oxidatively modified retinal proteins in a chronic pressure-induced rat model of glaucoma. Invest. Ophthalmol. Vis. Sci. 46, 3177-3187 (Pubitemid 44264303)
62. Tezel, G., Yang, X., Luo, C., Kain, A., Powell, D., Kuehn, M. H., and Kaplan, H. J. (2010) Oxidative stress and the regulation of complement activation in human glaucoma. Invest. Ophthalmol. Vis. Sci. 51, 5071-5082
63. Yu, A. L., Fuchshofer, R., Birke, M., Kampik, A., Bloemendal, H., and Welge-Lüssen, U. (2008) Oxidative stress and TGF-beta2 increase heat shock protein 27 expression in human optic nerve head astrocytes. Invest. Ophthalmol. Vis. Sci. 49, 5403-5411
64. Hernandez, M. R., Igoe, F., and Neufeld, A. H. (1988) Cell culture of the human lamina cribrosa. Invest. Ophthalmol. Vis. Sci. 29, 78-89 (Pubitemid 18038774)
65. Lambert, W., Agarwal, R., Howe, W., Clark, A. F., and Wordinger, R. J. (2001) Neurotrophin and neurotrophin receptor expression by cells of the human lamina cribrosa. Invest. Ophthalmol. Vis. Sci. 42, 2315-2323 (Pubitemid 32848861)
66. Applied Biosystem Technical Note (2010) Multiplex protein quantitation using iTRAQ reagents-8plex labelling efficiency, dynamc range, and reproducibility. 1, 1-5
67. Ross, P. L., Huang, Y. N., Marchese, J. N., Williamson, B., Parker, K., Hattan, S., Khainovski, N., Pillai, S., Dey, S., Daniels, S., Purkayastha, S., Juhasz, P., Martin, S., Bartlet-Jones, M., He, F., Jacobson, A., and Pappin, D. J. (2004) Multiplexed protein quantitation in Saccharomyces cerevisiae using amine-reactive isobaric tagging reagents. Mol. Cell. Proteomics 3, 1154-1169
68. Shilov, I. V., Seymour, S. L., Patel, A. A., Loboda, A., Tang, W. H., Keating, S. P., Hunter, C. L., Nuwaysir, L. M., and Schaeffer, D. A. (2007) The Paragon Algorithm, a next generation search engine that uses sequence temperature values and feature probabilities to identify peptides from tandem mass spectra. Mol. Cell. Proteomics 6, 1638-1655 (Pubitemid 47506173)
69. Eng, L. F. (1985) Glial fibrillary acidic protein (GFAP): the major protein of glial intermediate filaments in differentiated astrocytes. J. Neuroimmunol. 8, 203-214
70. Steely, H. T., Jr., English-Wright, S. L., and Clark, A. F. (2000) The similarity of protein expression in trabecular meshwork and lamina cribrosa: implications for glaucoma. Exp. Eye Res. 70, 17-30 (Pubitemid 30046871)
71. Wanner, I. B., Deik, A., Torres, M., Rosendahl, A., Neary, J. T., Lemmon, V. P., and Bixby, J. L. (2008) A new in vitro model of the glial scar inhibits axon growth. Glia 56, 1691-1709
72. Araujo, H., Danziger, N., Cordier, J., Glowinski, J., and Chneiweiss, H. (1993) Characterization of PEA-15, a major substrate for protein kinase C in astrocytes. J. Biol. Chem. 268, 5911-5920 (Pubitemid 23090927)
73. Renault, F., Formstecher, E., Callebaut, I., Junier, M. P., and Chneiweiss, H. (2003) The multifunctional protein PEA-15 is involved in the control of apoptosis and cell cycle in astrocytes. Biochem. Pharmacol. 66, 1581-1588 (Pubitemid 38373349)
74. Sharif, A., Renault, F., Beuvon, F., Castellanos R., Canton, B., Barbeito, L., Junier, M. P., and Chneiweiss, H. (2004) The expression of PEA-15 (phosphoprotein enriched in astrocytes of 15 kDa) defines subpopulations of astrocytes and neurons throughout the adult mouse brain. Neuroscience 126, 263-275 (Pubitemid 38780802)
75. Spicer, A. P., Kaback, L. A., Smith, T. J., and Seldin, M. F. (1998) Molecular cloning and characterization of the human and mouse UDP-glucose dehydrogenase genes. J. Biol. Chem. 273, 25117-25124 (Pubitemid 28443270)
76. Binari, R. C., Staveley, B. E., Johnson, W. A., Godavarti, R., Sasisekharan, R., and Manoukian, A. S. (1997) Genetic evidence that heparin-like glycosaminoglycans are involved in wingless signaling. Development 124, 2623-2632 (Pubitemid 27341762)
77. Clarkin, C. E., Allen, S., Kuiper, N. J., Wheeler, B. T., Wheeler-Jones, C. P., and Pitsillides, A. A. (2011) Regulation of UDP-glucose dehydrogenase is sufficient to modulate hyaluronan production and release, control sulfated GAG synthesis, and promote chondrogenesis. J. Cell. Physiol. 226, 749-761
78. Bontemps, Y., Vuillermoz, B., Antonicelli, F., Perreau, C., Danan, J. L., Maquart, F. X., and Wegrowski, Y. (2003) Specific protein-1 is a universal regulator of UDP-glucose dehydrogenase expression: its positive involvement in transforming growth factor-beta signaling and inhibition in hypoxia. J. Biol. Chem. 278, 21566-21575 (Pubitemid 36792555)
79. Egger, S., Chaikuad, A., Kavanagh, K. L., Oppermann, U., and Nidetzky, B. (2010) UDP-glucose dehydrogenase: structure and function of a potential drug target. Biochem. Soc. Trans. 38, 1378-1385
80. Tait, J. F., Smith, C., Frankenberry, D. A., Miao, C. H., Adler, D. A., and Disteche, C. M. (1992) Chromosomal mapping of the human annexin IV (ANX4) gene. Genomics 12, 313-318
81. Yang, Z., Quigley, H. A., Pease, M. E., Yang, Y., Qian, J., Valenta, D., and Zack, D. J. (2007) Changes in gene expression in experimental glaucoma and optic nerve transection: the equilibrium between protective and detrimental mechanisms. Invest. Ophthalmol. Vis. Sci. 48, 5539-5548 (Pubitemid 351260855)
82. Semov, A., Moreno, M. J., Onichtchenko, A., Abulrob, A., Ball, M., Ekiel, I., Pietrzynski, G., Stanimirovic, D., and Alakhov, V. (2005) Metastasisassociated protein S100A4 induces angiogenesis through interaction with Annexin II and accelerated plasmin formation. J. Biol. Chem. 280, 20833-20841 (Pubitemid 40776788)
83. Rothhut, B. (1997) Participation of annexins in protein phosphorylation. Cell. Mol. Life Sci. 53, 522-526
84. Edwards, H. C., and Crumpton, M. J. (1991) Ca(2+)-dependent phospholipid and arachidonic acid binding by the placental annexins VI and IV. Eur. J. Biochem. 198, 121-129
85. Jeon, Y. J., Kim, D. H., Jung, H., Chung, S. J., Chi, S. W., Cho, S., Lee, S. C., Park, B. C., Park, S. G., and Bae, K. H. (2010) Annexin A4 interacts with the NF-kappaB p50 subunit and modulates NF-kappaB transcriptional activity in a Ca2+-dependent manner. Cell. Mol. Life Sci. 67, 2271-2281
86. Ghosh, S., May, M. J., and Kopp, E. B. (1998) NF-kappa B and Rel proteins: evolutionarily conserved mediators of immune responses. Annu. Rev. Immunol. 16, 225-260 (Pubitemid 28183365)
87. Baldwin, A. S. (2001) Control of oncogenesis and cancer therapy resistance by the transcription factor NF-kappaB. J. Clin. Invest. 107, 241-246
88. Li, C., Zhang, F., and Wang, Y. (2010) S100A proteins in the pathogenesis of experimental corneal neovascularization. Mol. Vis. 16, 2225-2235
89. Heizmann, C. W., Fritz, G., and Schafer, B. W. (2002) S100 proteins: structure, functions and pathology. Front Biosci. 7, 1356-1368
90. Marenholz, I., Heizmann, C. W., and Fritz, G. (2004) S100 proteins in mouse and man: from evolution to function and pathology (including an update of the nomenclature). Biochem. Biophys. Res. Commun. 322, 1111-1122 (Pubitemid 39164640)
91. de Silva Rudland, S., Martin, L., Roshanlall, C., Winstanley, J., Leinster, S., Platt-Higgins, A., Carroll, J., West, C., Barraclough, R., and Rudland, P. (2006) Association of S100A4 and osteopontin with specific prognostic factors and survival of patients with minimally invasive breast cancer. Clin. Cancer Res. 12, 1192-1200 (Pubitemid 43342508)
92. Lambros, M. P., Parsa, C., Mulamalla, H., Orlando, R., Lau, B., Huang, Y., Pon, D., and Chow, M. (2011) Identifying cell and molecular stress after radiation in a three-dimensional (3-D) model of oral mucositis. Biochem. Biophys. Res. Commun. 405, 102-106
93. Wan, D., Gong, Y., Qin, W., Zhang, P., Li, J., Wei, L., Zhou, X., Li, H., Qiu, X., Zhong, F., He, L., Yu, J., Yao, G., Jiang, H., Qian, L., Yu, Y., Shu, H., Chen, X., Xu, H., Guo, M., Pan, Z., Chen, Y., Ge, C., Yang, S., and Gu, J. (2004) Large-scale cDNA transfection screening for genes related to cancer development and progression. Proc. Natl. Acad. Sci. U.S.A. 101, 15724-15729 (Pubitemid 39473555)
94. Ihanamaki, T., Pelliniemi, L. J., and Vuorio, E. (2004) Collagens and collagen- related matrix components in the human and mouse eye. Prog. Retin. Eye Res. 23, 403-434 (Pubitemid 38829450)
95. Nakagawa, T., Tanaka, Y., Matsuoka, E., Kondo, S., Okada, Y., Noda, Y., Kanai, Y., and Hirokawa, N. (1997) Identification and classification of 16 new kinesin superfamily (KIF) proteins in mouse genome. Proc. Natl. Acad. Sci. U.S.A., 94, 9654-9659 (Pubitemid 27408117)
96. Robles-Valero, J., Martín-Cófreces, N. B., Lamana, A., Macdonald, S., Volkov, Y., and Sánchez-Madrid, F. (2010) Integrin and CD3/TCR activation are regulated by the scaffold protein AKAP450. Blood 115, 4174-4184
97. Greiner, T. C., Dasgupta, C., Ho, V. V., Weisenburger, D. D., Smith, L. M., Lynch, J. C., Vose, J. M., Fu, K., Armitage, J. O., Braziel, R. M., Campo, E., Delabie, J., Gascoyne, R. D., Jaffe, E. S., Muller-Hermelink, H. K., Ott, G., Rosenwald, A., Staudt, L. M., Im, M. Y., Karaman, M. W., Pike, B. L., Chan, W. C., and Hacia, J. G. (2006) Mutation and genomic deletion status of ataxia telangiectasia mutated (ATM) and p53 confer specific gene expression profiles in mantle cell lymphoma. Proc. Natl. Acad. Sci. U.S.A. 103, 2352-2357 (Pubitemid 43271673)
98. Rosty, C., Sheffer, M., Tsafrir, D., Stransky, N., Tsafrir, I., Peter, M., de Crémoux, P., de La Rochefordière, A., Salmon, R., Dorval, T., Thiery, J. P., Couturier, J., Radvanyi, E., Domany, F., and Sastre-Garau, X. (2005) Identification of a proliferation gene cluster associated with HPV E6/E7 expression level and viral DNA load in invasive cervical carcinoma. Oncogene 24, 7094-7104 (Pubitemid 41573638)
99. Krasnoselsky, A. L., Whiteford, C. C., Wei, J. S., Bilke, S., Westermann, F., Chen, Q. R., and Khan, J. (2005) Altered expression of cell cycle genes distinguishes aggressive neuroblastoma. Oncogene 24, 1533-1541 (Pubitemid 40396083)
100. Reimann, J. D., Freed, E., Hsu, J. Y., Kramer, E. R., Peters, J. M., and Jackson, P. K. (2001) Emi1 is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex. Cell 105, 645-655 (Pubitemid 32524117)
101. Carracedo, A., Ito, K., and Pandolfi, P. P. (2011) The nuclear bodies inside out: PML conquers the cytoplasm. Curr. Opin. Cell Biol. 23, 360-366
102. Thorleifsson, G., Magnusson, K. P., Sulem, P., Walters, G. B., Gudbjartsson, D. F., Stefansson, H., Jonsson, T., Jonasdottir, A., Stefansdottir, G., Masson, G., Hardarson, G. A., Petursson, H., Arnarsson, A., Motallebipour, M., Wallerman, O., Wadelius, C., Gulcher, J. R., Thorsteinsdottir, U., Kong, A., Jonasson, F., and Stefansson, K. (2007) Common sequence variants in the LOXL1 gene confer susceptibility to exfoliation glaucoma. Science 317, 1397-1400 (Pubitemid 47417481)
103. Wolff, S., Otto, A., Albrecht, D., Zeng, J. S., Büttner, K., Glückmann, M., Hecker, M., and Becher, D. (2006) Gel-free and gel-based proteomics in Bacillus subtilis: a comparative study. Mol. Cell. Proteomics 5, 1183-1192 (Pubitemid 44103390)
104. Shadforth, I. P., Dunkley, T. P., Lilley, K. S., and Bessant, C. (2005) i-Tracker: for quantitative proteomics using iTRAQ. BMC Genomics. 6, 145
105. Köcher, T., Pichler, P., Schutzbier, M., Stingl, C., Kaul, A., Teucher, N., Hasenfuss, G., Penninger, J. M., and Mechtler, K. (2009) High precision quantitative proteomics using iTRAQ on an LTQ Orbitrap: a new mass spectrometric method combining the benefits of all. J. Proteome Res. 8, 4743-4752