Protein expression in a transformed trabecular meshwork cell line: Proteome analysis

Molecular Vision

Volumn 12, Issue , 2006, Pages 372-383

  Steely, H Thomas ^a   Dillow, Glen W ^a   Bian, Liangqian ^a   Grundstad, Jason ^b   Braun, Terry A ^b   Casavant, Thomas L ^b   McCartney, Mitchell D ^a   Clark, Abbot F ^a  

^a ALCON RESEARCH LTD   (United States)

^b UNIVERSITY OF IOWA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANAZOLENE SODIUM; PEPTIDE DERIVATIVE; PROTEOME; UREA;

ALGORITHM; AMINO ACID SEQUENCE; ARTICLE; BIOINFORMATICS; CALCULATION; CELL LINE; CELL TRANSFORMATION; COLLOID; CONTROLLED STUDY; DERIVATIZATION; ELECTROSPRAY MASS SPECTROMETRY; EXTRACTION; GEL; GLAUCOMA; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; INFORMATION PROCESSING; INTERNET; ISOELECTRIC FOCUSING; LIQUID CHROMATOGRAPHY; MOLECULAR WEIGHT; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DATABASE; PROTEIN EXPRESSION; PROTEIN IMMOBILIZATION; REDUCTION; SOLUBILIZATION; TISSUE CULTURE; TRABECULAR MESHWORK; TWO DIMENSIONAL GEL ELECTROPHORESIS; UNINDEXED SEQUENCE;

CELL LINE, TRANSFORMED; CHROMATOGRAPHY, LIQUID; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; EYE PROTEINS; HUMANS; MASS SPECTROMETRY; MOLECULAR WEIGHT; PROTEOME; PROTEOMICS; TRABECULAR MESHWORK;

EID: 33645829833     PISSN: 10900535     EISSN: 10900535     Source Type: Journal    
DOI: None     Document Type: Article

References (67)

1. Harvey PT. Common eye diseases of elderly people: Identifying and treating causes of vision loss. Gerontology 2003; 49:1-11.
2. Thylefors B, Negrel AD, Pararajasegaram R, Dadzie KY. Global data on blindness. Bull World Health Organ 1995; 73:115-21.
3. Thylefors B, Negrel AD. The global impact of glaucoma. Bull World Health Organ 1994; 72:323-6.
4. Frezzotti R, Renieri A, Frezzotti P. Adult-onset primary glaucoma and molecular genetics: A review. Eur J Ophthalmol 2004; 14:220-5.
5. Ray K, Mukhopadhyay A, Acharya M. Recent advances in molecular genetics of glaucoma. Mol Cell Biochem 2003; 253:223-31.
6. Libby RT, Gould DB, Anderson MG, John SW. Complex genetics of glaucoma susceptibility. Annu Rev Genomics Hum Genet 2005; 6:15-44.
7. Gordon MO, Beiser JA, Brandt JD, Heuer DK, Higginbotham EJ, Johnson CA, Keltner JL, Miller JP, Parrish RK 2nd, Wilson MR, Kass MA. The Ocular Hypertension Treatment Study: Baseline factors that predict the onset of primary open-angle glaucoma. Arch Ophthalmol 2002; 120:714-20; discussion 829-30.
8. The Advanced Glaucoma Intervention Study (AGIS): 7. The relationship between control of intraocular pressure and visual field deterioration. The AGIS Investigators. Am J Ophthalmol 2000; 130:429-40.
9. Feiner L, Piltz-Seymour JR, Collaborative Initial Glaucoma Treatment Study. Collaborative Initial Glaucoma Treatment Study: A summary of results to date. Curr Opin Ophthalmol 2003; 14:106-11.
10. Rohen JW. New studies on the functional morphology of the trabecular meshwork and the outflow channels. Trans Ophthalmol Soc U K 1970; 89:431-47.
11. Lutjen-Drecoll E. Functional morphology of the trabecular meshwork in primate eyes. Prog Retin Eye Res 1999; 18:91-119.
12. Acott TS. Trabecular extracellular matrix regulation. In: Drance SM, Van Buskirk EM, Neufeld AH, editors. Pharmacology of glaucoma. Baltimore: Williams & Wilkins; 1992. p. 125-57.
13. Acott TS. Biochemistry of aqueous outflow. In: Podos SM, Yanoff M, editors. Textbook of Ophthalmology. Vol 7. London: Mosby; 1994. p. 1.47-1.78.
14. Grierson I, Howes RC. Age-related depletion of the cell population in the human trabecular meshwork. Eye 1987; 1:204-10.
15. Rohen JW, Lutjen-Drecoll E, Flugel C, Meyer M, Grierson I. Ultrastructure of the trabecular meshwork in untreated cases of primary open-angle glaucoma (POAG). Exp Eye Res 1993; 56:683-92.
16. Yun AJ, Murphy CG, Polansky JR, Newsome DA, Alvarado JA. Proteins secreted by human trabecular cells. Glucocorticoid and other effects. Invest Ophthalmol Vis Sci 1989; 30:2012-22.
17. Clark AF, Wilson K, de Kater AW, Allingham RR, McCartney MD. Dexamethasone-induced ocular hypertension in perfusion-cultured human eyes. Invest Ophthalmol Vis Sci 1995; 36:478-89.
18. Knepper PA, Goossens W, Hvizd M, Palmberg PF. Glycosaminoglycans of the human trabecular meshwork in primary openangle glaucoma. Invest Ophthalmol Vis Sci 1996; 37:1360-7.
19. Yue BY. The extracellular matrix and its modulation in the trabecular meshwork. Surv Ophthalmol 1996; 40:379-90.
20. Banks RE, Dunn MJ, Hochstrasser DF, Sanchez JC, Blackstock W, Pappin DJ, Selby PJ. Proteomics: New perspectives, new biomedical opportunities. Lancet 2000; 356:1749-56.
21. Polansky JR, Weinreb RN, Baxter JD, Alvarado J. Human trabecular cells. I. Establishment in tissue culture and growth characteristics. Invest Ophthalmol Vis Sci 1979; 18:1043-9.
22. Wordinger RJ, Clark AF. Effects of glucocorticoids on the trabecular meshwork: Towards a better understanding of glaucoma. Prog Retin Eye Res 1999; 18:629-67.
23. Pang IH, Shade DL, Clark AF, Steely HT, DeSantis L. Preliminary characterization of a transformed cell strain derived from human trabecular meshwork. Curr Eye Res 1994; 13:51-63.
24. Blackstock WP, Weir MP. Proteomics: Quantitative and physical mapping of cellular proteins. Trends Biotechnol 1999; 17:121-7.
25. Appella E, Arnott D, Sakaguchi K, Wirth PJ. Proteome mapping by two-dimensional polyacrylamide gel electrophoresis in combination with mass spectrometric protein sequence analysis. EXS 2000; 88:1-27.
26. Steely HT Jr, English-Wright SL, Clark AF. The similarity of protein expression in trabecular meshwork and lamina cribrosa: Implications for glaucoma. Exp Eye Res 2000; 70:17-30.
27. Brown RE, Jarvis KL, Hyland KJ. Protein measurement using bicinchoninic acid: Elimination of interfering substances. Anal Biochem 1989; 180:136-9.
28. Gorg A, Boguth G, Obermaier C, Posch A, Weiss W. Two-dimensional polyacrylamide gel electrophoresis with immobilized pH gradients in the first dimension (IPG-Dalt): The state of the art and the controversy of vertical versus horizontal systems. Electrophoresis 1995; 16:1079-86.
29. Rabilloud T, Valette C, Lawrence JJ. Sample application by ingel rehydration improves the resolution of two-dimensional electrophoresis with immobilized pH gradients in the first dimension. Electrophoresis 1994; 15:1552-8.
30. Hochstrasser DF, Harrington MG, Hochstrasser AC, Miller MJ, Merril CR. Methods for increasing the resolution of two-dimensional protein electrophoresis. Anal Biochem 1988; 173:424-35.
31. Neuhoff V, Stamm R, Eibl H. Clear background and highly sensitive protein staining with Coomassie Blue dyes in polyacrylamide gels: A systematic analysis. Electrophoresis 1985; 6:427-48.
32. Myrick JE, Lemkin PF, Robinson MK, Upton KM. Comparison of the Bio Image Visage 2000 and the GELLAB-II two-dimensional electrophoresis image analysis systems. Appl Theor Electrophor 1993; 3:335-46.
33. Mirza UA, Liu YH, Tang JT, Porter F, Bondoc L, Chen G, Pramanik BN, Nagabhushan TL. Extraction and characterization of adenovirus proteins from sodium dodecylsulfate polyacrylamide gel electrophoresis by matrix-assisted laser desorption/ionization mass spectrometry. J Am Soc Mass Spectrom 2000; 11:356-61.
34. Shevchenko A, Wilm M, Vorm O, Mann M. Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels. Anal Chem 1996; 68:850-8.
35. Yamashita M, Fenn JB. Electrospray ion source. Another variation on the free-jet theme. J Phys Chem 1984; 88:4451-9.
36. Eng JK, McCormack AL, Yates JR III. An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database. J Am Soc Mass Spectrom 1994; 5:976-89.
37. Clark AF, Wilson K, McCartney MD, Miggans ST, Kunkle M, Howe W. Glucocorticoid-induced formation of cross-linked actin networks in cultured human trabecular meshwork cells. Invest Ophthalmol Vis Sci 1994; 35:281-94.
38. Tamm ER, Siegner A, Baur A, Lutjen-Drecoll E. Transforming growth factor-beta 1 induces alpha-smooth muscle-actin expression in cultured human and monkey trabecular meshwork. Exp Eye Res 1996; 62:389-97.
39. Flugel C, Tamm E, Lutjen-Drecoll E. Different cell populations in bovine trabecular meshwork: An ultrastructural and immunocytochemical study. Exp Eye Res 1991; 52:681-90.
40. Tengroth B, Ammitzboll T. Changes in the content and composition of collagen in the glaucomatous eye basis for a new hypothesis for the genesis of chronic open angle glaucoma - a preliminary report. Acta Ophthalmol (Copenh) 1984; 62:999-1008.
41. Rehnberg M, Ammitzboll T, Tengroth B. Collagen distribution in the lamina cribrosa and the trabecular meshwork of the human eye. Br J Ophthalmol 1987; 71:886-92.
42. Floyd BB, Cleveland PH, Worthen DM. Fibronectin in human trabecular drainage channels. Invest Ophthalmol Vis Sci 1985; 26:797-804.
43. Hernandez MR, Weinstein BI, Schwartz J, Ritch R, Gordon GG, Southren AL. Human trabecular meshwork cells in culture: Morphology and extracellular matrix components. Invest Ophthalmol Vis Sci 1987; 28:1655-60.
44. Murphy CG, Yun AJ, Newsome DA, Alvarado JA. Localization of extracellular proteins of the human trabecular meshwork by indirect immunofluorescence. Am J Ophthalmol 1987; 104:33-43.
45. Zhou L, Zhang SR, Yue BY. Adhesion of human trabecular meshwork cells to extracellular matrix proteins. Roles and distribution of integrin receptors. Invest Ophthalmol Vis Sci 1996; 37:104-13.
46. Wirtz MK, Bradley JM, Xu H, Domreis J, Nobis CA, Truesdale AT, Samples JR, Van Buskirk EM, Acott TS. Proteoglycan expression by human trabecular meshworks. Curr Eye Res 1997; 16:412-21.
47. Wordinger RJ, Clark AF, Agarwal R, Lambert W, McNatt L, Wilson SE, Qu Z, Fung BK. Cultured human trabecular meshwork cells express functional growth factor receptors. Invest Ophthalmol Vis Sci 1998; 39:1575-89.
48. Zhou L, Maruyama I, Li Y, Cheng EL, Yue BY. Expression of integrin receptors in the human trabecular meshwork. Curr Eye Res 1999; 19:395-402.
49. Wordinger RJ, Agarwal R, Talati M, Fuller J, Lambert W, Clark AF. Expression of bone morphogenetic proteins (BMP), BMP receptors, and BMP associated proteins in human trabecular meshwork and optic nerve head cells and tissues. Mol Vis 2002; 8:241-50.
50. Bhattacharya SK, Rockwood EJ, Smith SD, Bonilha VL, Crabb JS, Kuchtey RW, Robertson NG, Peachey NS, Morton CC, Crabb JW. Proteomics reveal Cochlin deposits associated with glaucomatous trabecular meshwork. J Biol Chem 2005; 280:6080-4.
51. Zhao X, Ramsey KE, Stephan DA, Russell P. Gene and protein expression changes in human trabecular meshwork cells treated with transforming growth factor-beta. Invest Ophthalmol Vis Sci 2004; 45:4023-34.
52. Santoni V, Molloy M, Rabilloud T. Membrane proteins and proteomics: Un amour impossible? Electrophoresis 2000; 21:1054-70.
53. Tamm ER, Russell P, Piatigorsky J. Development of characterization of a immortal and differentiated murine trabecular meshwork cell line. Invest Ophthalmol Vis Sci 1999; 40:1392-403.
54. Dennis G Jr, Sherman BT, Hosack DA, Yang J, Gao W, Lane HC, Lempicki RA. DAVID: Database for Annotation, Visualization, and Integrated Discovery. Genome Biol 2003; 4:P3.
55. Stone EM, Fingert JH, Alward WL, Nguyen TD, Polansky JR, Sunden SL, Nishimura D, Clark AF, Nystuen A, Nichols BE, Mackey DA, Ritch R, Kalenak JW, Craven ER, Sheffield VC. Identification of a gene that causes primary open angle glaucoma. Science 1997; 275:668-70.
56. Rezaie T, Child A, Hitchings R, Brice G, Miller L, Coca-Prados M, Heon E, Krupin T, Ritch R, Kreutzer D, Crick RP, Sarfarazi M. Adult-onset primary open-angle glaucoma caused by mutations in optineurin. Science 2002; 295:1077-9.
57. Monemi S, Spaeth G, DaSilva A, Popinchalk S, Ilitchev E, Liebmann J, Ritch R, Heon E, Crick RP, Child A, Sarfarazi M. Identification of a novel adult-onset primary open-angle glaucoma (POAG) gene on 5q22.1. Hum Mol Genet 2005; 14:725-33.
58. Friedman JS, Faucher M, Hiscott P, Biron VL, Malenfant M, Turcotte P, Raymond V, Walter MA. Protein localization in the human eye and genetic screen of opticin. Hum Mol Genet 2002; 11:1333-42.
59. Tamm ER, Russell P, Epstein DL, Johnson DH, Piatigorsky J. Modulation of myocilin/TIGR expression in human trabecular meshwork. Invest Ophthalmol Vis Sci 1999; 40:2577-82.
60. Hojrup P, Roepstorff P, Houen G. Human placental calreticulin characterization of domain structure and post-translational modifications. Eur J Biochem 2001; 268:2558-65.
61. Denning GM, Leidal KG, Holst VA, Iyer SS, Pearson DW, Clark JR, Nauseef WM, Clark RA. Calreticulin biosynthesis and processing in human myeloid cells: Demonstration of signal peptide cleavage and N-glycosylation. Blood 1997; 90:372-81.
62. Peterson JR, Ora A, Van PN, Helenius A. Transient, lectin-like association of calreticulin with folding intermediates of cellular and viral glycoproteins. Mol Biol Cell 1995; 6:1173-84.
63. Jacobson N, Andrews M, Shepard AR, Nishimura D, Searby C, Fingert JH, Hageman G, Mullins R, Davidson BL, Kwon YH, Alward WL, Stone EM, Clark AF, Sheffield VC. Non-secretion of mutant proteins of the glaucoma gene myocilin in cultured trabecular meshwork cells and in aqueous humor. Hum Mol Genet 2001; 10:117-25.
64. Liu Y, Vollrath D. Reversal of mutant myocilin non-secretion and cell killing: Implications for glaucoma. Hum Mol Genet 2004; 13:1193-204.
65. Pratt WB, Galigniana MD, Morishima Y, Murphy PJ. Role of molecular chaperones in steroid receptor action. Essays Biochem 2004; 40:41-58.
66. Clark AF, Morrison JM. Corticosteroid glaucoma. In: Morrison JC, Pollack IP editors. Glaucoma: Science and practice. New York: Thieme; 2003. p. 197-206.
67. White IR, Pickford R, Wood J, Skehel JM, Gangadharan B, Cutler P. A statistical comparison of silver and SYPRO Ruby staining for proteomic analysis. Electrophoresis 2004; 25:3048-54.