Genome-wide landscape of liver X receptor chromatin binding and gene regulation in human macrophages

BMC Genomics

Volumn 13, Issue 1, 2012, Pages

  Pehkonen, Petri ^a   Welter Stahl, Lynn ^b   Diwo, Janine ^b   Ryynänen, Jussi ^a   Wienecke Baldacchino, Anke ^b   Heikkinen, Sami ^a   Treuter, Eckardt ^c   Steffensen, Knut R ^c   Carlberg, Carsten ^a  

^a UNIVERSITY OF EASTERN FINLAND   (Finland)

^b UNIVERSITY OF LUXEMBOURG   (Luxembourg)

^c KAROLINSKA INSTITUTE   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

LIVER X RECEPTOR; MESSENGER RNA; PHORBOL 13 ACETATE 12 MYRISTATE; ORPHAN NUCLEAR RECEPTOR;

ANTIBODY SPECIFICITY; APOPTOSIS; ARTICLE; BINDING SITE; CHROMATIN IMMUNOPRECIPITATION; CHROMOSOMAL LOCALIZATION; CONTROLLED STUDY; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENE IDENTIFICATION; GENETIC ASSOCIATION; HUMAN; HUMAN CELL; LIPID METABOLISM; LIPID TRANSPORT; MACROPHAGE; MICROARRAY ANALYSIS; NUCLEOTIDE SEQUENCE; PROTEIN FUNCTION; RECEPTOR BINDING; RECEPTOR DOWN REGULATION; RECEPTOR UPREGULATION; SEQUENCE ANALYSIS; CELL LINE; CHROMATIN; DNA MICROARRAY; DOWN REGULATION; DRUG ANTAGONISM; METABOLISM; PROTEIN BINDING; UPREGULATION;

APOPTOSIS; BINDING SITES; CELL LINE; CHROMATIN; DOWN-REGULATION; GENE EXPRESSION REGULATION; GENOME-WIDE ASSOCIATION STUDY; HUMANS; LIPID METABOLISM; MACROPHAGES; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; ORPHAN NUCLEAR RECEPTORS; PROTEIN BINDING; UP-REGULATION;

EID: 84856316797     PISSN: None     EISSN: 14712164     Source Type: Journal    
DOI: 10.1186/1471-2164-13-50     Document Type: Article

References (73)

1. Apfel R, Benbrook D, Lernhardt E, Ortiz MA, Salbert G, Pfahl M. A novel orphan receptor specific for a subset of thyroid hormone-responsive elements and its interaction with the retinoid/thyroid hormone receptor subfamily. Mol Cell Biol 1994, 14:7025-7035. 359232, 7935418.
2. Janowski BA, Willy PJ, Devi TR, Falck JR, Mangelsdorf DJ. An oxysterol signalling pathway mediated by the nuclear receptor LXRα. Nature 1996, 383:728-731. 10.1038/383728a0, 8878485.
3. Willy PJ, Umesono K, Ong ES, Evans RM, Heyman RA, Mangelsdorf DJ. LXR, a nuclear receptor that defines a distinct retinoid response pathway. Genes Dev 1995, 9:1033-1045. 10.1101/gad.9.9.1033, 7744246.
4. Peet DJ, Turley SD, Ma W, Janowski BA, Lobaccaro JM, Hammer RE, Mangelsdorf DJ. Cholesterol and bile acid metabolism are impaired in mice lacking the nuclear oxysterol receptor LXRα. Cell 1998, 93:693-704. 10.1016/S0092-8674(00)81432-4, 9630215.
5. Joseph SB, Bradley MN, Castrillo A, Bruhn KW, Mak PA, Pei L, Hogenesch J, O'Connell R M, Cheng G, Saez E, Miller JF, Tontonoz P. LXR-dependent gene expression is important for macrophage survival and the innate immune response. Cell 2004, 119:299-309. 10.1016/j.cell.2004.09.032, 15479645.
6. Bensinger SJ, Bradley MN, Joseph SB, Zelcer N, Janssen EM, Hausner MA, Shih R, Parks JS, Edwards PA, Jamieson BD, Tontonoz P. LXR signaling couples sterol metabolism to proliferation in the acquired immune response. Cell 2008, 134:97-111. 10.1016/j.cell.2008.04.052, 2626438, 18614014.
7. Töröcsik D, Barath M, Benko S, Szeles L, Dezso B, Poliska S, Hegyi Z, Homolya L, Szatmari I, Lanyi A, Nagy L. Activation of liver X receptor sensitizes human dendritic cells to inflammatory stimuli. J Immunol 2010, 184:5456-5465. 10.4049/jimmunol.0902399, 20410489.
8. Chawla A, Boisvert WA, Lee C, Laffitte BA, Barak Y, Joseph SB, Liao D, Nagy L, Edwards PA, Curtiss LK, Evans RM, Tontonoz P. A PPARγ-LXR-ABCA1 pathway in macrophages is involved in cholesterol efflux and atherogenesis. Mol Cell 2001, 7:161-171. 10.1016/S1097-2765(01)00164-2, 11172721.
9. Joseph SB, McKilligin E, Pei L, Watson MA, Collins AR, Laffitte BA, Chen M, Noh G, Goodman J, Hagger GN, Tran J, Tippin TK, Wang X, Lusis AJ, Hsueh WA, Law RE, Collins JL, Willson TM, Tontonoz P. Synthetic LXR ligand inhibits the development of atherosclerosis in mice. Proc Natl Acad Sci USA 2002, 99:7604-7609. 10.1073/pnas.112059299, 124297, 12032330.
10. Castrillo A, Joseph SB, Vaidya SA, Haberland M, Fogelman AM, Cheng G, Tontonoz P. Crosstalk between LXR and toll-like receptor signaling mediates bacterial and viral antagonism of cholesterol metabolism. Mol Cell 2003, 12:805-816. 10.1016/S1097-2765(03)00384-8, 14580333.
11. Ghisletti S, Huang W, Ogawa S, Pascual G, Lin ME, Willson TM, Rosenfeld MG, Glass CK. Parallel SUMOylation-dependent pathways mediate gene- and signal-specific transrepression by LXRs and PPARγ. Mol Cell 2007, 25:57-70. 10.1016/j.molcel.2006.11.022, 1850387, 17218271.
12. Repa JJ, Turley SD, Lobaccaro JA, Medina J, Li L, Lustig K, Shan B, Heyman RA, Dietschy JM, Mangelsdorf DJ. Regulation of absorption and ABC1-mediated efflux of cholesterol by RXR heterodimers. Science 2000, 289:1524-1529. 10.1126/science.289.5484.1524, 10968783.
13. Schultz JR, Tu H, Luk A, Repa JJ, Medina JC, Li L, Schwendner S, Wang S, Thoolen M, Mangelsdorf DJ, Lustig KD, Shan B. Role of LXRs in control of lipogenesis. Genes Dev 2000, 14:2831-2838. 10.1101/gad.850400, 317060, 11090131.
14. Quack M, Frank C, Carlberg C. Differential nuclear receptor signalling from DR4-type response elements. J Cell Biochem 2002, 86:601-612. 10.1002/jcb.10247, 12210766.
15. Luo Y, Tall AR. Sterol upregulation of human CETP expression in vitro and in transgenic mice by an LXR element. J Clin Invest 2000, 105:513-520. 10.1172/JCI8573, 289164, 10683381.
16. Kaplan R, Zhang T, Hernandez M, Gan FX, Wright SD, Waters MG, Cai TQ. Regulation of the angiopoietin-like protein 3 gene by LXR. J Lipid Res 2003, 44:136-143. 10.1194/jlr.M200367-JLR200, 12518032.
17. Uehara Y, Miura S, von Eckardstein A, Abe S, Fujii A, Matsuo Y, Rust S, Lorkowski S, Assmann G, Yamada T, Saku K. Unsaturated fatty acids suppress the expression of the ATP-binding cassette transporter G1 (ABCG1) and ABCA1 genes via an LXR/RXR responsive element. Atherosclerosis 2007, 191:11-21. 10.1016/j.atherosclerosis.2006.04.018, 16730733.
18. Heinz S, Benner C, Spann N, Bertolino E, Lin YC, Laslo P, Cheng JX, Murre C, Singh H, Glass CK. Simple combinations of lineage-determining transcription factors prime cis-regulatory elements required for macrophage and B cell identities. Mol Cell 2010, 38:576-589. 10.1016/j.molcel.2010.05.004, 2898526, 20513432.
19. Boergesen M, Pedersen TA, Gross B, van Heeringen SJ, Hagenbeek D, Bindesbøll C, Caron S, Lalloyer F, Steffensen KR, Nebb H, Gustafsson JA, Stunnenberg HG, Staels B, Mandrup S. Genome-wide profiling of LXR, RXR and PPARα in mouse liver reveals extensive sharing of binding sites. Mol Cell Biol 2012, 32:852-867.
20. Jakobsson T, Venteclef N, Toresson G, Damdimopoulos AE, Ehrlund A, Lou X, Sanyal S, Steffensen KR, Gustafsson JA, Treuter E. GPS2 is required for cholesterol efflux by triggering histone demethylation, LXR recruitment, and coregulator assembly at the ABCG1 locus. Mol Cell 2009, 34:510-518. 10.1016/j.molcel.2009.05.006, 19481530.
21. Venteclef N, Jakobsson T, Ehrlund A, Damdimopoulos A, Mikkonen L, Ellis E, Nilsson LM, Parini P, Jänne OA, Gustafsson JA, Steffensen KR, Treuter E. GPS2-dependent corepressor/SUMO pathways govern anti-inflammatory actions of LRH-1 and LXRβ in the hepatic acute phase response. Genes Dev 2010, 24:381-395. 10.1101/gad.545110, 2816737, 20159957.
22. Langmead B, Trapnell C, Pop M, Salzberg SL. Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. Genome Biol 2009, 10:R25. 10.1186/gb-2009-10-3-r25, 2690996, 19261174.
23. Zhang Y, Liu T, Meyer CA, Eeckhoute J, Johnson DS, Bernstein BE, Nusbaum C, Myers RM, Brown M, Li W, Liu XS. Model-based analysis of ChIP-Seq (MACS). Genome Biol 2008, 9:R137. 10.1186/gb-2008-9-9-r137, 2592715, 18798982.
24. Bailey TL, Elkan C. Fitting a mixture model by expectation maximization to discover motifs in biopolymers. Proc Int Conf Intell Syst Mol Biol 1994, 2:28-36.
25. Gupta S, Stamatoyannopoulos JA, Bailey TL, Noble WS. Quantifying similarity between motifs. Genome Biol 2007, 8:R24. 10.1186/gb-2007-8-2-r24, 1852410, 17324271.
26. Turatsinze JV, Thomas-Chollier M, Defrance M, van Helden J. Using RSAT to scan genome sequences for transcription factor binding sites and cis-regulatory modules. Nat Protoc 2008, 3:1578-1588. 10.1038/nprot.2008.97, 18802439.
27. Landrier JF, Grober J, Demydchuk J, Besnard P. FXRE can function as an LXRE in the promoter of human ileal bile acid-binding protein (I-BABP) gene. FEBS Lett 2003, 553:299-303. 10.1016/S0014-5793(03)01033-0, 14572640.
28. Guillon N, Tirode F, Boeva V, Zynovyev A, Barillot E, Delattre O. The oncogenic EWS-FLI1 protein binds in vivo GGAA microsatellite sequences with potential transcriptional activation function. PLoS One 2009, 4:e4932. 10.1371/journal.pone.0004932, 2654724, 19305498.
29. Thiagalingam A, De Bustros A, Borges M, Jasti R, Compton D, Diamond L, Mabry M, Ball DW, Baylin SB, Nelkin BD. RREB-1, a novel zinc finger protein, is involved in the differentiation response to Ras in human medullary thyroid carcinomas. Mol Cell Biol 1996, 16:5335-5345. 231532, 8816445.
30. Lebson L, Gocke A, Rosenzweig J, Alder J, Civin C, Calabresi PA, Whartenby KA. Cutting edge: The transcription factor Kruppel-like factor 4 regulates the differentiation of Th17 cells independently of RORγt. J Immunol 185:7161-7164.
31. Venkateswaran A, Laffitte BA, Joseph SB, Mak PA, Wilpitz DC, Edwards PA, Tontonoz P. Control of cellular cholesterol efflux by the nuclear oxysterol receptor LXRα. Proc Natl Acad Sci USA 2000, 97:12097-12102. 10.1073/pnas.200367697, 17300, 11035776.
32. Cuddapah S, Jothi R, Schones DE, Roh TY, Cui K, Zhao K. Global analysis of the insulator binding protein CTCF in chromatin barrier regions reveals demarcation of active and repressive domains. Genome Res 2009, 19:24-32. 2612964, 19056695.
33. Houck KA, Borchert KM, Hepler CD, Thomas JS, Bramlett KS, Michael LF, Burris TP. T0901317 is a dual LXR/FXR agonist. Mol Genet Metab 2004, 83:184-187. 10.1016/j.ymgme.2004.07.007, 15464433.
34. Mitro N, Vargas L, Romeo R, Koder A, Saez E. T0901317 is a potent PXR ligand: implications for the biology ascribed to LXR. FEBS Lett 2007, 581:1721-1726. 10.1016/j.febslet.2007.03.047, 17418145.
35. Kumar N, Solt LA, Conkright JJ, Wang Y, Istrate MA, Busby SA, Garcia-Ordonez RD, Burris TP, Griffin PR. The benzenesulfoamide T0901317 [N-(2,2,2-trifluoroethyl)-N-[4-[2,2,2-trifluoro-1-hydroxy-1-(trifluorometh yl)ethyl]phenyl]-benzenesulfonamide] is a novel retinoic acid receptor-related orphan receptor-alpha/gamma inverse agonist. Mol Pharmacol 2010, 77:228-236. 10.1124/mol.109.060905, 2812071, 19887649.
36. Chuu CP, Chen RY, Hiipakka RA, Kokontis JM, Warner KV, Xiang J, Liao S. The liver X receptor agonist T0901317 acts as androgen receptor antagonist in human prostate cancer cells. Biochem Biophys Res Commun 2007, 357:341-346. 10.1016/j.bbrc.2007.03.116, 2693411, 17416342.
37. Dennis G, Sherman BT, Hosack DA, Yang J, Gao W, Lane HC, Lempicki RA. DAVID: Database for Annotation, Visualization, and Integrated Discovery. Genome Biol 2003, 4:P3. 10.1186/gb-2003-4-5-p3, 12734009.
38. Bolker B, Bonebakker L, Gentleman R, Huber W, Liaw A, Lumley T, Maechler M, Magnusson A, Moeller S, Schwartz M, Venables B. gplots: various R programming tools for plotting data. R package version 280 2010,
39. Moras D, Gronemeyer H. The nuclear receptor ligand-binding domain: structure and function. Curr Opin Cell Biol 1998, 10:384-391. 10.1016/S0955-0674(98)80015-X, 9640540.
40. Shen Q, Bai Y, Chang KC, Wang Y, Burris TP, Freedman LP, Thompson CC, Nagpal S. Liver X receptor-retinoid X receptor (LXR-RXR) heterodimer cistrome reveals co-ordination of LXR and AP1 signaling in keratinocytes. J Biol Chem 2011, 286:14554-14563. 10.1074/jbc.M110.165704, 21349840.
41. Heikkinen S, Väisänen S, Pehkonen P, Seuter S, Benes V, Carlberg C. Nuclear hormone 1α,25-dihydroxyvitamin D3 elicts a genome-wide shift in the locations of VDR chromatin accupancy. Nucl Acids Res 2011, 39:9181-9193. 10.1093/nar/gkr654, 3241659, 21846776.
42. Ramagopalan SV, Heger A, Berlanga AJ, Maugeri NJ, Lincoln MR, Burrell A, Handunnetthi L, Handel AE, Disanto G, Orton SM, Watson CT, Morahan JM, Giovannoni G, Ponting CP, Ebers GC, Knight JC. A ChIP-seq defined genome-wide map of vitamin D receptor binding: associations with disease and evolution. Genome Res 2010, 20:1352-1360. 10.1101/gr.107920.110, 2945184, 20736230.
43. Heikkinen S, Seuter S, Carlberg C. The first genome-wide view on vitamin D receptor locations and their mechanistic implications. Anticancer Res 2012, 32:271-282.
44. Väisänen S, Dunlop TW, Sinkkonen L, Frank C, Carlberg C. Spatio-temporal activation of chromatin on the human CYP24 gene promoter in the presence of 1α,25-dihydroxyvitamin D3. J Mol Biol 2005, 350:65-77. 10.1016/j.jmb.2005.04.057, 15919092.
45. Saramäki A, Banwell CM, Campbell MJ, Carlberg C. Regulation of the human p21(waf1/cip1) gene promoter via multiple binding sites for p53 and the vitamin D3 receptor. Nucleic Acids Res 2006, 34:543-554. 10.1093/nar/gkj460, 1351372, 16434701.
46. Meyer MB, Goetsch PD, Pike JW. A downstream intergenic cluster of regulatory enhancers contributes to the induction of CYP24A1 Expression by 1α,25-dihydroxyvitamin D3. J Biol Chem 2010, 285:15599-15610. 10.1074/jbc.M110.119958, 2865326, 20236932.
47. Hotokezaka Y, van Leyen K, Lo EH, Beatrix B, Katayama I, Jin G, Nakamura T. alphaNAC depletion as an initiator of ER stress-induced apoptosis in hypoxia. Cell Death Differ 2009, 16:1505-1514. 10.1038/cdd.2009.90, 19609276.
48. Pratt WB, Morishima Y, Murphy M, Harrell M. Chaperoning of glucocorticoid receptors. Handb Exp Pharmacol 2006, 111-138.
49. Callahan MK, Ransohoff RM. Analysis of leukocyte extravasation across the blood-brain barrier: conceptual and technical aspects. Curr Allergy Asthma Rep 2004, 4:65-73. 10.1007/s11882-004-0046-9, 14680625.
50. Igl W, Johansson A, Wilson JF, Wild SH, Polasek O, Hayward C, Vitart V, Hastie N, Rudan P, Gnewuch C, Schmitz G, Meitinger T, Pramstaller PP, Hicks AA, Oostra BA, van Duijn CM, Rudan I, Wright A, Campbell H, Gyllensten U. Modeling of environmental effects in genome-wide association studies identifies SLC2A2 and HP as novel loci influencing serum cholesterol levels. PLoS Genet 2010, 6:e1000798. 10.1371/journal.pgen.1000798, 2792712, 20066028.
51. Wright KO, Messing EM, Reeder JE. Increased expression of the acid sphingomyelinase-like protein ASML3a in bladder tumors. J Urol 2002, 168:2645-2649. 10.1016/S0022-5347(05)64236-X, 12442002.
52. Liu X, Steffensen KR, Sanna A, Arru G, Fois ML, Rosati G, Sotgiu S, Link H, Gustafsson JA, Huang YM. Anti-inflammatory nuclear receptor superfamily in multiple sclerosis patients from Sardinia and Sweden. Neurobiol Dis 2005, 20:961-968. 10.1016/j.nbd.2005.06.001, 16023861.
53. Giorelli M, Livrea P, Minervini MG, Trojano M. Immunomodulatory properties of increased levels of liver X receptor beta in peripheral blood mononuclear cells from multiple sclerosis patients. Exp Neurol 2007, 204:759-766. 10.1016/j.expneurol.2007.01.013, 17337267.
54. Chintalacharuvu SR, Sandusky GE, Burris TP, Burmer GC, Nagpal S. Liver X receptor is a therapeutic target in collagen-induced arthritis. Arthritis Rheum 2007, 56:1365-1367. 10.1002/art.22528, 17393442.
55. Asquith DL, Miller AM, Hueber AJ, McKinnon HJ, Sattar N, Graham GJ, McInnes IB. Liver X receptor agonism promotes articular inflammation in murine collagen-induced arthritis. Arthritis Rheum 2009, 60:2655-2665. 10.1002/art.24717, 19714646.
56. Hindinger C, Hinton DR, Kirwin SJ, Atkinson RD, Burnett ME, Bergmann CC, Stohlman SA. Liver X receptor activation decreases the severity of experimental autoimmune encephalomyelitis. J Neurosci Res 2006, 84:1225-1234. 10.1002/jnr.21038, 16955483.
57. Joseph SB, Castrillo A, Laffitte BA, Mangelsdorf DJ, Tontonoz P. Reciprocal regulation of inflammation and lipid metabolism by liver X receptors. Nat Med 2003, 9:213-219. 10.1038/nm820, 12524534.
58. Seres L, Cserepes J, Elkind NB, Torocsik D, Nagy L, Sarkadi B, Homolya L. Functional ABCG1 expression induces apoptosis in macrophages and other cell types. Biochim Biophys Acta 2008, 1778:2378-2387. 10.1016/j.bbamem.2008.06.010, 18619413.
59. Choe SS, Choi AH, Lee JW, Kim KH, Chung JJ, Park J, Lee KM, Park KG, Lee IK, Kim JB. Chronic activation of liver X receptor induces beta-cell apoptosis through hyperactivation of lipogenesis: liver X receptor-mediated lipotoxicity in pancreatic beta-cells. Diabetes 2007, 56:1534-1543. 10.2337/db06-1059, 17369526.
60. Mehrotra A, Kaul D, Joshi K. LXR-alpha selectively reprogrammes cancer cells to enter into apoptosis. Mol Cell Biochem 2011, 349:41-55. 10.1007/s11010-010-0659-3, 21125317.
61. Pommier AJ, Alves G, Viennois E, Bernard S, Communal Y, Sion B, Marceau G, Damon C, Mouzat K, Caira F, Baron S, Lobaccaro JM. Liver X Receptor activation downregulates AKT survival signaling in lipid rafts and induces apoptosis of prostate cancer cells. Oncogene 2010, 29:2712-2723. 10.1038/onc.2010.30, 20190811.
62. Toresson G, Schuster GU, Steffensen KR, Bengtsson M, Ljunggren J, Dahlman-Wright K, Gustafsson JA. Purification of functional full-length liver X receptor beta produced in Escherichia coli. Protein Expr Purif 2004, 35:190-198. 10.1016/j.pep.2004.01.007, 15135392.
63. Pehkonen P, Heikkinen S, Carlberg C. Analysis of genome-wide data on transcription factor binding and their target gene regulation: a case study on liver X receptors. Proc Int Workshop Comp Sys Biol 2011, 2011:149-152.
64. Salmon-Divon M, Dvinge H, Tammoja K, Bertone P. PeakAnalyzer: genome-wide annotation of chromatin binding and modification loci. BMC Bioinformatics 2010, 11:415. 10.1186/1471-2105-11-415, 2923140, 20691053.
65. Kent WJ, Sugnet CW, Furey TS, Roskin KM, Pringle TH, Zahler AM, Haussler D. The human genome browser at UCSC. Genome Res 2002, 12:996-1006. 186604, 12045153.
66. R-Development-Core-Team R: A language and environment for statistical computing. R Foundation for Statistical Computing Vienna, Austria, ISBN 2009, 3-900051-900007-900050, R-Development-Core-Team.
67. Gentleman RC, Carey VJ, Bates DM, Bolstad B, Dettling M, Dudoit S, Ellis B, Gautier L, Ge Y, Gentry J, Hornik K, Hothorn T, Huber W, Iacus S, Irizarry R, Leisch F, Li C, Maechler M, Rossini AJ, Sawitzki G, Smith C, Smyth G, Tierney L, Yang JY, Zhang J. Bioconductor: open software development for computational biology and bioinformatics. Genome Biol 2004, 5:R80. 10.1186/gb-2004-5-10-r80, 545600, 15461798.
68. Smyth GK. Linear models and empirical bayes methods for assessing differential expression in microarray experiments. Stat Appl Genet Mol Biol 2004, 3. Article 3.
69. Benjamini Y, Hochberg Y. Controlling the false discovery rate: a practical and powerful approach to multiple testing. J R Statist Soc B 1995, 57:289-300.
70. Ashburner M, Ball CA, Blake JA, Botstein D, Butler H, Cherry JM, Davis AP, Dolinski K, Dwight SS, Eppig JT, Harris MA, Hill DP, Issel-Tarver L, Kasarskis A, Lewis S, Matese JC, Richardson JE, Ringwald M, Rubin GM, Sherlock G. Gene ontology: tool for the unification of biology. The Gene Ontology Consortium. Nat Genet 2000, 25:25-29. 10.1038/75556, 3037419, 10802651.
71. Picard F, Robin S, Lavielle M, Vaisse C, Daudin JJ. A statistical approach for array CGH data analysis. BMC Bioinformatics 2005, 6:27. 10.1186/1471-2105-6-27, 549559, 15705208.
72. Picard F, Robin S, Lebarbier E, Daudin JJ. A segmentation/clustering model for the analysis of array CGH data. Biometrics 2007, 63:758-766. 10.1111/j.1541-0420.2006.00729.x, 17825008.
73. Alberti S, Schuster G, Parini P, Feltkamp D, Diczfalusy U, Rudling M, Angelin B, Björkhem I, Pettersson S, Gustafsson JA. Hepatic cholesterol metabolism and resistance to dietary cholesterol in LXRbeta-deficient mice. J Clin Invest 2001, 107:565-573. 10.1172/JCI9794, 199420, 11238557.