Improvement of thermal stability via outer-loop ion pair interaction of mutated T1 lipase from geobacillus zalihae strain T1

International Journal of Molecular Sciences

Volumn 13, Issue 1, 2012, Pages 943-960

  Ruslan, Rudzanna ^a   Rahman, Raja Noor Zaliha Raja Abd ^a   Leow, Thean Chor ^a   Ali, Mohd Shukuri Mohamad ^a   Basri, Mahiran ^a   Salleh, Abu Bakar ^a  

^a UNIVERSITY PUTRA MALAYSIA   (Malaysia)

Author keywords

CD spectral analysis; Ion pair interaction; Lipase crystal; Thermal stability; Thermostable lipase; X ray diffraction

Indexed keywords

MUTANT PROTEIN; T1 LIPASE; TRIACYLGLYCEROL LIPASE; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; ION; RECOMBINANT PROTEIN;

ALPHA HELIX; AMINO ACID COMPOSITION; ARTICLE; BACTERIAL STRAIN; CHEMICAL PROCEDURES; CIRCULAR DICHROISM; CONTROLLED STUDY; CRYSTALLIZATION; ENZYME ANALYSIS; ENZYME ISOLATION; ENZYME PURIFICATION; ENZYME STABILITY; GEOBACILLUS; GEOBACILLUS ZALIHAE; HYDROGEN BOND; INTER LOOP NETWORK; INTRA LOP NETWORK; MELTING TEMPERATURE; MUTATION; NONHUMAN; PROTEIN CONFORMATION; PROTEIN INTERACTION; PROTEIN SECONDARY STRUCTURE; PROTEIN STRUCTURE; PROTEIN UNFOLDING; SITE DIRECTED MUTAGENESIS; SITTING DROP VAPOR DIFFUSION METHOD; STRUCTURE ANALYSIS; TEMPERATURE; TEMPERATURE SENSITIVITY; THERMOSTABILITY; X RAY DIFFRACTION; BIOSYNTHESIS; CHEMISTRY; ENZYMOLOGY; GENETICS; METABOLISM; PROTEIN TERTIARY STRUCTURE; TRANSITION TEMPERATURE; X RAY CRYSTALLOGRAPHY;

GEOBACILLUS;

BACTERIAL PROTEINS; CIRCULAR DICHROISM; CRYSTALLOGRAPHY, X-RAY; ENZYME STABILITY; GEOBACILLUS; HYDROGEN BONDING; IONS; LIPASE; MUTAGENESIS, SITE-DIRECTED; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; TEMPERATURE; TRANSITION TEMPERATURE;

EID: 84856242067     PISSN: None     EISSN: 14220067     Source Type: Journal    
DOI: 10.3390/ijms13010943     Document Type: Article

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