Increasing stability of water-soluble PQQ glucose dehydrogenase by increasing hydrophobic interaction at dimeric interface

BMC Biochemistry

Volumn 6, Issue , 2005, Pages

  Tanaka, Shunsuke ^a   Igarashi, Satoshi ^a   Ferri, Stefano ^a   Sode, Koji ^a  

^a TOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ASPARAGINE; BACTERIAL ENZYME; GLUCOSE DEHYDROGENASE (PYRROLOQUINOLINEQUINONE); ISOLEUCINE; LEUCINE; OXIDOREDUCTASE; PHENYLALANINE; THREONINE; TYROSINE; UNCLASSIFIED DRUG; VALINE;

AMINO ACID SUBSTITUTION; ARTICLE; CATALYSIS; DIMERIZATION; ENZYME ACTIVITY; ENZYME BINDING; ENZYME STABILITY; ENZYME STRUCTURE; HYDROPHOBICITY; MOLECULAR INTERACTION; MUTANT; PHYSICAL CHEMISTRY; PROTEIN QUATERNARY STRUCTURE; SITE DIRECTED MUTAGENESIS; TEMPERATURE DEPENDENCE; THERMOSTABILITY; WILD TYPE;

ACTINOBACTERIA; DIMERIZATION; ENZYME STABILITY; GLUCOSE DEHYDROGENASES; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; SOLUBILITY; SURFACE PROPERTIES; WATER;

ACINETOBACTER CALCOACETICUS;

EID: 15444364022     PISSN: 14712091     EISSN: 14712091     Source Type: Journal    
DOI: 10.1186/1471-2091-6-1     Document Type: Article

References (26)

1. D'Costa EJ, Higgins IJ, Turner APF: Quinoprotein glucose dehydrogenase and its application in an amperometric glucose sensor. Biosensors 1986, 2:71-87.
2. Yokoyama K, Sode K, Tamiya E, Karube I: Integrated biosensor for glucose and galactose. Anal Chim Acta 1989, 218:137-142.
3. Smolander M, Livio H-L, Rasanen L: Mediated amperometric determination of xylose and glucose with an immobilized aldose dehydrogenase electrode. Biosensors & Bioelectronics 1992, 7:637-643.
4. Sode K, Nakasono S, Tanaka M, Matsunaga T: Subzero temperature operating biosensor utilizing an organic solvent and quinoprotein glucose dehydrogenase. Biotechnol Bioeng 1993, 42:251-254.
5. Ye L, Hammerle M, Olsthoorn AJJ, Schuhmann W, Schmidt H-L, Duine JA, Heller A: High current density "wired" quinoprotein glucose dehydrogenase electrode. Anal Chem 1993, 65:238-241.
6. Katz E, Schlereth DD, Schmidt H-L: Reconstitution of the quinoprotein glucose dehydrogenase from its apoenzyme on a gold electrode surface modified with a monolayer of pyrroloquinoline quinone. Electroanal Chem 1994, 368:165-171.
7. Kost GJ, Vu H-T, Lee JH, Bourgeois P, Kiechle FL, Martin C, Miller SS, Okorodudu AO, Podczasy JJ, Webster R, Whitlow KJ: Multi-center study of oxygen-insensitive handheld glucose point-of-care testing in critical care/hospital/ambulatory patients in the United States and Canada. Crit Care Med 1998, 26:581-590.
8. Laurinavicius V, Kurtinaitiene B, Liauksminas V, Jankauskaite A, Simkus R, Meskys R, Boguslavsky L, Skotheim T, Tanenbaum S: Reagentless biosensor based on PQQ-dependent glucose dehydrogenase and partially hydrolyzed polyarbutin. Talanta 2000, 52:485-493.
9. Razumiene J, Meskys R, Gureviciene V, Laurinavicius V, Reshetova MD, Ryabov AD: 4-Ferrocenyllphenol as an electron transfer mediator in PQQ-dependent alcohol and glucose dehydrogenase-catalyzed reactions. Electrochemistry Commun 2000, 2:307-311.
10. Schmidt B: Oxygen-independent oxidases A new class of enzymes for application in diagnostics. Clinica Chim Acta 1997, 26:33-37.
11. Mullen WH, Churchhouse J, Vadgama P: Enzyme electrode for glucose based on the quinoprotein glucose dehydrogenase. Analyst 1985, 110:925-928.
12. Takahashi Y, Igarashi S, Nakazawa Y, Tsugawa W, Sode K: Construction and characterization of glucose enzyme sensor employing engineered water soluble PQQ glucose dehydrogenase with improved thermal stability. Electrochemistry 2000, 68:907-911.
13. Sode K, Shirahane M, Yoshida H: Construction and characterization of a linked-dimeric pyrroloquinoline quinone glucose dehydrogenase. Biotechnol Lett 1999, 21:707-710.
14. Igarashi I, Sode K: Stabilization of quaternary structure of water-soluble quinoprotein glucose dehydrogenase. Mol Biotech 2003, 24:97-103.
15. Sode K, Ohtera T, Shirahane M, Witarto AB, Igarashi S, Yoshida H: Increasing the thermal stability of the water-soluble pyrroloquinoline quinone glucose dehydrogenase by single amino acid replacement. Enz Microbial Technol 2000, 26:491-496.
16. Igarashi S, Ohtera T, Yoshida H, Witarto AB, Sode K: Construction and characterization of mutant water-soluble PQQ glucose dehydrogenases with altered Km value - site-directed mutagenesis studies on the putative active site. Biochem Biophys Res Commun 1999, 264:820-824.
17. Igarashi S, Okuda J, Ikebukuro K, Sode K: Molecular engineering of PQQGDH and its applications. Arch Biochem Biophys 2004, 428:52-63.
18. Oubrie A, Rozeboom HJ, Kalk KH, Duine JA, Dijkstra BW: The 1.7Å crystal structure of the apo-form of the soluble quinoprotein glucose dehydrogenase from Acinetobacter calcoaceticus reveals a novel internal conserved sequence repeat. J Mol Biol 1999, 289:319-333.
19. Faber HR, Groom CR, Baker HM, Morgan WT, Smith A, Baker EN: 1.8Å crystal structure of the C-terminal domain of rabbit serum haemopexin. Structure 1995, 3:551-559.
20. Oubrie A, Rozeboom HJ, Kalk KH, Olsthoorn AJJ, Duine JA, Dijkstra BW: Structure and mechanism of soluble quinoprotein glucose dehydrogenase. EMBO J 1999, 18:5187-5159.
21. Kallwass HKW, Surewicz W, Parris W, Macfarlane ELA, Luyten MA, Kay CM, Gold M, Jones JB: Single amino acid substitution can further increase the stability of a thermophilic L-lactate dehydrogenase. Protein Eng 1992, 5:769-774.
22. Kirino H, Aoki M, Aoshima M, Hayashi Y, Ohba M, Yamagishi A, Wakagi T, Oshima T: Hydrophobic interaction at the subunit interface contributes to the thermostability of 3-isopropylmalate dehydrogenase from an extreme thermophile, Thermus thermophilus. Eur J Biochem 1994, 220:275-281.
23. Akanuma S, Yamagishi A, Tanaka N, Oshima T: Serial increase in the thermal stability of 3-isopropylmalate dehydrogenase from Bacillus subtilis by experimental evolution. Protein Sci 1998, 7:698-705.
24. Ohkuri T, Yamagishi A: Increased thermal stability against irreversible inactivation of 3-isopropylmalate dehydrogenase induced by decreased van der Waals volume at the subunit interface. Protein Eng 2003, 16:615-621.
25. Glaser F, Steinberg DM, Vakser IA, Ben-Tal N: Residue frequencies and pairing preferences at protein-protein interfaces. Proteins 2001, 43:89-102.
26. Sayle S, Milner-White EJ: RasMol: Biomolecular graphics for all Trends in Biochemical Sciences. TIBS 1995, 20:374.