메뉴 건너뛰기




Volumn 393, Issue 1-2, 2012, Pages 23-35

Dye selection for live cell imaging of intact siRNA

Author keywords

Confocal fluorescence microscopy; Fluorescence resonance energy transfer (FRET); RNA interference (RNAi); SiRNA degradation; SiRNA integrity; Small interfering RNA (siRNA)

Indexed keywords

ENHANCED GREEN FLUORESCENT PROTEIN; FLUORESCENT DYE; OLIGONUCLEOTIDE; SMALL INTERFERING RNA;

EID: 84856200163     PISSN: 14316730     EISSN: 14374315     Source Type: Journal    
DOI: 10.1515/BC-2011-256     Document Type: Article
Times cited : (13)

References (43)
  • 1
    • 34447306000 scopus 로고    scopus 로고
    • Effect of siRNA nuclease stability on the in vitro and in vivo kinetics of siRNA-mediated gene silencing
    • DOI 10.1002/bit.21285
    • Bartlett, D.W. and Davis, M.E. (2007). Effect of siRNA nuclease stability on the in vitro and in vivo kinetics of siRNA-mediated gene silencing. Biotechnol. Bioeng. 97, 909-921. (Pubitemid 47051060)
    • (2007) Biotechnology and Bioengineering , vol.97 , Issue.4 , pp. 909-921
    • Bartlett, D.W.1    Davis, M.E.2
  • 2
    • 79953232011 scopus 로고    scopus 로고
    • Effective siRNA delivery and target mRNA degradation using an amphipathic peptide to facilitate pH-dependent endosomal escape
    • Bartz, R., Fan, H., Zhang, J., Innocent, N., Cherrin, C., Beck, S.C., Pei, Y., Momose, A., Jadhav, V., Tellers, D.M., et al. (2011). Effective siRNA delivery and target mRNA degradation using an amphipathic peptide to facilitate pH-dependent endosomal escape. Biochem. J. 435, 475-487.
    • (2011) Biochem. J. , vol.435 , pp. 475-487
    • Bartz, R.1    Fan, H.2    Zhang, J.3    Innocent, N.4    Cherrin, C.5    Beck, S.C.6    Pei, Y.7    Momose, A.8    Jadhav, V.9    Tellers, D.M.10
  • 4
    • 0036752785 scopus 로고    scopus 로고
    • RNAi in human cells: Basic structural and functional features of small interfering RNA
    • DOI 10.1016/S1097-2765(02)00652-4
    • Chiu, Y.L. and Rana, T.M. (2002). RNAi in human cells: basic structural and functional features of small interfering RNA. Mol. Cell 10, 549-561. (Pubitemid 35284173)
    • (2002) Molecular Cell , vol.10 , Issue.3 , pp. 549-561
    • Chiu, Y.-L.1    Rana, T.M.2
  • 5
    • 4344651353 scopus 로고    scopus 로고
    • Visualizing a correlation between siRNA localization, cellular uptake, and RNAi in living cells
    • DOI 10.1016/j.chembiol.2004.06.006, PII S1074552104001930
    • Chiu, Y.L., Ali, A., Chu, C.Y., Cao, H., and Rana, T.M. (2004). Visualizing a correlation between siRNA localization, cellular uptake, and RNAi in living cells. Chem. Biol. 11, 1165-1175. (Pubitemid 39138769)
    • (2004) Chemistry and Biology , vol.11 , Issue.8 , pp. 1165-1175
    • Chiu, Y.-L.1    Ali, A.2    Chu, C.-Y.3    Cao, H.4    Rana, T.M.5
  • 6
    • 70349992906 scopus 로고    scopus 로고
    • Design and development of IT-101, a cyclodextrin- containing polymer conjugate of camptothecin
    • Davis, M.E. (2009). Design and development of IT-101, a cyclodextrin- containing polymer conjugate of camptothecin. Adv. Drug Deliv. Rev. 61, 1189-1192.
    • (2009) Adv. Drug Deliv. Rev. , vol.61 , pp. 1189-1192
    • Davis, M.E.1
  • 8
    • 59849125915 scopus 로고    scopus 로고
    • Phosphorothioate-stimulated cellular uptake of siRNA: A cell culture model for mechanistic studies
    • Detzer, A., Overhoff, M., Mescalchin, A., Rompf, M., and Sczakiel, G. (2008). Phosphorothioate-stimulated cellular uptake of siRNA: a cell culture model for mechanistic studies. Curr. Pharm. Des. 14, 3666-3673.
    • (2008) Curr. Pharm. Des. , vol.14 , pp. 3666-3673
    • Detzer, A.1    Overhoff, M.2    Mescalchin, A.3    Rompf, M.4    Sczakiel, G.5
  • 9
    • 0036351317 scopus 로고    scopus 로고
    • Analysis of gene function in somatic mammalian cells using small interfering RNAs
    • DOI 10.1016/S1046-2023(02)00023-3, PII S1046202302000233
    • Elbashir, S.M., Harborth, J., Weber, K., and Tuschl, T. (2002). Analysis of gene function in somatic mammalian cells using small interfering RNAs. Methods 26, 199-213. (Pubitemid 34969582)
    • (2002) Methods , vol.26 , Issue.2 , pp. 199-213
    • Elbashir, S.M.1    Harborth, J.2    Weber, K.3    Tuschl, T.4
  • 10
    • 77953140972 scopus 로고    scopus 로고
    • Argonaute proteins at a glance
    • Ender, C. and Meister, G. (2010). Argonaute proteins at a glance. J. Cell Sci. 123, 1819 - 1823.
    • (2010) J. Cell Sci. , vol.123 , pp. 1819-1823
    • Ender, C.1    Meister, G.2
  • 12
    • 31544433310 scopus 로고    scopus 로고
    • The RNA silencing pathway: The bits and pieces that matter
    • Groenenboom, M.A., Maree, A.F., and Hogeweg, P. (2005). The RNA silencing pathway: the bits and pieces that matter. PLoS Comput. Biol. 1, 155-165.
    • (2005) PLoS Comput. Biol. , vol.1 , pp. 155-165
    • Groenenboom, M.A.1    Maree, A.F.2    Hogeweg, P.3
  • 14
    • 79957755711 scopus 로고    scopus 로고
    • SiRNA-like double-stranded RNAs are specifically protected against degradation in human cell extract
    • Hoerter, J.A., Krishnan, V., Lionberger, T.A., and Walter, N.G. (2011). siRNA-like double-stranded RNAs are specifically protected against degradation in human cell extract. PLoS One 6, e20359.
    • (2011) PLoS One , vol.6
    • Hoerter, J.A.1    Krishnan, V.2    Lionberger, T.A.3    Walter, N.G.4
  • 15
    • 63049105426 scopus 로고    scopus 로고
    • Localization of doublestranded small interfering RNA to cytoplasmic processing bodies is Ago2 dependent and results in up-regulation of GW182 and Argonaute-2
    • Jagannath, A. and Wood, M.J. (2009). Localization of doublestranded small interfering RNA to cytoplasmic processing bodies is Ago2 dependent and results in up-regulation of GW182 and Argonaute-2. Mol. Biol. Cell 20, 521-529.
    • (2009) Mol. Biol. Cell , vol.20 , pp. 521-529
    • Jagannath, A.1    Wood, M.J.2
  • 17
    • 77951677584 scopus 로고    scopus 로고
    • Upconversion nanoparticle-based FRET system for study of siRNA in live cells
    • Jiang, S. and Zhang, Y. (2010). Upconversion nanoparticle-based FRET system for study of siRNA in live cells. Langmuir 26, 6689-6694.
    • (2010) Langmuir , vol.26 , pp. 6689-6694
    • Jiang, S.1    Zhang, Y.2
  • 18
    • 47249138782 scopus 로고    scopus 로고
    • Mechanisms and strategies for effective delivery of antisense and siRNA oligonucleotides
    • DOI 10.1093/nar/gkn342
    • Juliano, R., Alam, M.R., Dixit, V., and Kang, H. (2008). Mechanisms and strategies for effective delivery of antisense and siRNA oligonucleotides. Nucleic Acids Res. 36, 4158-4171. (Pubitemid 351984831)
    • (2008) Nucleic Acids Research , vol.36 , Issue.12 , pp. 4158-4171
    • Juliano, R.1    Alam, Md.R.2    Dixit, V.3    Kang, H.4
  • 19
    • 84856188088 scopus 로고    scopus 로고
    • Cellular uptake and intracellular trafficking of antisense and siRNA oligonucleotides
    • [Epub ahead of print]
    • Juliano, R.L., Ming, X., and Nakagawa, O. (2011). Cellular uptake and intracellular trafficking of antisense and siRNA oligonucleotides. Bioconjug. Chem. [Epub ahead of print].
    • (2011) Bioconjug. Chem
    • Juliano, R.L.1    Ming, X.2    Nakagawa, O.3
  • 21
    • 79751485564 scopus 로고    scopus 로고
    • The many faces of RNAi
    • Ketting, R.F. (2011). The many faces of RNAi. Dev. Cell 20, 148-161.
    • (2011) Dev. Cell , vol.20 , pp. 148-161
    • Ketting, R.F.1
  • 23
    • 79959479529 scopus 로고    scopus 로고
    • Mechanisms of single-stranded phosphorothioate modifi ed antisense oligonucleotide accumulation in hepatocytes
    • Koller, E., Vincent, T.M., Chappell, A., De, S., Manoharan, M., and Bennett, C.F. (2011). Mechanisms of single-stranded phosphorothioate modifi ed antisense oligonucleotide accumulation in hepatocytes. Nucleic Acids Res. 39, 4795-4807.
    • (2011) Nucleic Acids Res. , vol.39 , pp. 4795-4807
    • Koller, E.1    Vincent, T.M.2    S. De, C.A.3    Manoharan, M.4    Bennett, C.F.5
  • 24
    • 77049109902 scopus 로고    scopus 로고
    • Intracellular trafficking and unpacking of siRNA/quantum dot-PEI complexes modifi ed with and without cell penetrating peptide: Confocal and flow cytometric FRET analysis
    • Lee, H., Kim, I.K., and Park, T.G. (2010). Intracellular trafficking and unpacking of siRNA/quantum dot-PEI complexes modifi ed with and without cell penetrating peptide: confocal and flow cytometric FRET analysis. Bioconjug. Chem. 21, 289-295.
    • (2010) Bioconjug. Chem. , vol.21 , pp. 289-295
    • Lee, H.1    Kim, I.K.2    Park, T.G.3
  • 25
    • 59849111969 scopus 로고    scopus 로고
    • SiRNA modifications and subcellular localization: A question of intracellular transport?
    • Ohrt, T. and Schwille, P. (2008). siRNA modifications and subcellular localization: a question of intracellular transport? Curr. Pharm. Des. 14, 3674-3685.
    • (2008) Curr. Pharm. Des. , vol.14 , pp. 3674-3685
    • Ohrt, T.1    Schwille, P.2
  • 26
    • 33644992707 scopus 로고    scopus 로고
    • In situ fluorescence analysis demonstrates active SiRNA exclusion from the nucleus by exportin 5
    • Ohrt, T., Merkle, D., Birkenfeld, K., Echeverri, C.J., and Schwille, P. (2006). In situ fluorescence analysis demonstrates active siRNA exclusion from the nucleus by Exportin 5. Nucleic Acids Res. 34, 1369-1380.
    • (2006) Nucleic Acids Res. , vol.34 , pp. 1369-1380
    • Ohrt, T.1    Merkle, D.2    Birkenfeld, K.3    Echeverri, C.J.4    Schwille, P.5
  • 27
    • 56649122127 scopus 로고    scopus 로고
    • Fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy reveal the cytoplasmic origination of loaded nuclear RISC in vivo in human cells
    • Ohrt, T., Mutze, J., Staroske, W., Weinmann, L., Hock, J., Crell, K., Meister, G., and Schwille, P. (2008). Fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy reveal the cytoplasmic origination of loaded nuclear RISC in vivo in human cells. Nucleic Acids Res. 36, 6439-6449.
    • (2008) Nucleic Acids Res. , vol.36 , pp. 6439-6449
    • Ohrt, T.1    Mutze, J.2    Staroske, W.3    Weinmann, L.4    Hock, J.5    Crell, K.6    Meister, G.7    Schwille, P.8
  • 28
    • 79960306815 scopus 로고    scopus 로고
    • Fluorescence cross-correlation spectroscopy reveals mechanistic insights into the effect of 2'-O-methyl modifi ed siRNAs in living cells
    • Ohrt, T., Staroske, W., Mutze, J., Crell, K., Landthaler, M., and Schwille, P. (2011). Fluorescence cross-correlation spectroscopy reveals mechanistic insights into the effect of 2'-O-methyl modifi ed siRNAs in living cells. Biophys. J. 100, 2981-2990.
    • (2011) Biophys. J. , vol.100 , pp. 2981-2990
    • Ohrt, T.1    Staroske, W.2    Mutze, J.3    Crell, K.4    Landthaler, M.5    Schwille, P.6
  • 29
    • 0842346323 scopus 로고    scopus 로고
    • Long-lasting RNAi activity in mammalian neurons
    • DOI 10.1016/S0014-5793(04)00017-1
    • Omi, K., Tokunaga, K., and Hohjoh, H. (2004). Long-lasting RNAi activity in mammalian neurons. FEBS Lett. 558, 89-95. (Pubitemid 38167416)
    • (2004) FEBS Letters , vol.558 , Issue.1-3 , pp. 89-95
    • Omi, K.1    Tokunaga, K.2    Hohjoh, H.3
  • 30
    • 79960618381 scopus 로고    scopus 로고
    • Balancing ER dynamics: Shaping, bending, severing, and mending membranes
    • Pendin, D., McNew, J.A., and Daga, A. (2011). Balancing ER dynamics: shaping, bending, severing, and mending membranes. Curr. Opin. Cell Biol. 23, 435-442.
    • (2011) Curr. Opin. Cell Biol. , vol.23 , pp. 435-442
    • Pendin, D.1    McNew, J.A.2    Daga, A.3
  • 32
    • 6344249316 scopus 로고    scopus 로고
    • Dynamics of gene silencing by RNA interference
    • DOI 10.1002/bit.20216
    • Raab, R.M. and Stephanopoulos, G. (2004). Dynamics of gene silencing by RNA interference. Biotechnol. Bioeng. 88, 121-132. (Pubitemid 39388701)
    • (2004) Biotechnology and Bioengineering , vol.88 , Issue.1 , pp. 121-132
    • Raab, R.M.1    Stephanopoulos, G.2
  • 33
    • 33748253098 scopus 로고    scopus 로고
    • In situ analysis of single-stranded and duplex siRNA integrity in living cells
    • DOI 10.1021/bi060351b
    • Raemdonck, K., Remaut, K., Lucas, B., Sanders, N.N., Demeester, J., and De Smedt, S.C. (2006). In situ analysis of single-stranded and duplex siRNA integrity in living cells. Biochemistry 45, 10614-10623. (Pubitemid 44320476)
    • (2006) Biochemistry , vol.45 , Issue.35 , pp. 10614-10623
    • Raemdonck, K.1    Remaut, K.2    Lucas, B.3    Sanders, N.N.4    Demeester, J.5    De Smedt, S.C.6
  • 34
    • 77954314924 scopus 로고    scopus 로고
    • Prolonged gene silencing by combining siRNA nanogels and photochemical internalization
    • Raemdonck, K., Naeye, B., Hogset, A., Demeester, J., and De Smedt, S.C. (2010). Prolonged gene silencing by combining siRNA nanogels and photochemical internalization. J. Control. Release 145, 281-288.
    • (2010) J. Control. Release , vol.145 , pp. 281-288
    • Raemdonck, K.1    Naeye, B.2    Hogset, A.3    Demeester, J.4    De Smedt, S.C.5
  • 35
    • 13844274900 scopus 로고    scopus 로고
    • FRET-FCS as a tool to evaluate the stability of oligonucleotide drugs after intracellular delivery
    • DOI 10.1016/j.jconrel.2004.11.019, PII S0168365904005796
    • Remaut, K., Lucas, B., Braeckmans, K., Sanders, N.N., De Smedt, S.C., and Demeester, J. (2005). FRET-FCS as a tool to evaluate the stability of oligonucleotide drugs after intracellular delivery. J. Control. Release 103, 259-271. (Pubitemid 40248577)
    • (2005) Journal of Controlled Release , vol.103 , Issue.1 , pp. 259-271
    • Remaut, K.1    Lucas, B.2    Braeckmans, K.3    Sanders, N.N.4    De Smedt, S.C.5    Demeester, J.6
  • 36
    • 0028024646 scopus 로고
    • Regulation of gamma-glutamyl transpeptidase and alkaline phosphatase activities in immortalized rat brain microvessel endothelial cells
    • DOI 10.1002/jcp.1041590114
    • Roux, F., Durieu-Trautmann, O., Chaverot, N., Claire, M., Mailly, P., Bourre, J.M., Strosberg, A.D., and Couraud, P.O. (1994). Regulation of gamma-glutamyl transpeptidase and alkaline phosphatase activities in immortalized rat brain microvessel endothelial cells. J. Cell. Physiol. 159, 101-113. (Pubitemid 24234582)
    • (1994) Journal of Cellular Physiology , vol.159 , Issue.1 , pp. 101-113
    • Roux, F.1    Durieu-Trautmann, O.2    Chaverot, N.3    Claire, M.4    Mailly, P.5    Bourre, J.-M.6    Strosberg, A.D.7    Couraud, P.-O.8
  • 37
    • 79959233482 scopus 로고    scopus 로고
    • FRET-based probing to gain direct information on siRNA sustainability in live cells: Asymmetric degradation of siRNA strands
    • Shin, S., Kwon, H.M., Yoon, K.S., Kim, D.E., and Hah, S.S. (2011). FRET-based probing to gain direct information on siRNA sustainability in live cells: asymmetric degradation of siRNA strands. Mol. Biosyst. 7, 2110-2113.
    • (2011) Mol. Biosyst. , vol.7 , pp. 2110-2113
    • Shin, S.1    Kwon, H.M.2    Yoon, K.S.3    Kim, D.E.4    Hah, S.S.5
  • 38
    • 83555166124 scopus 로고    scopus 로고
    • Subcellular fate and off-target effects of siRNA, shRNA, and miRNA
    • Singh, S., Narang, A.S., and Mahato, R.I. (2011). Subcellular fate and off-target effects of siRNA, shRNA, and miRNA. Pharm. Res. 28, 2996-3015.
    • (2011) Pharm. Res. , vol.28 , pp. 2996-3015
    • Singh, S.1    Narang, A.S.2    Mahato, R.I.3
  • 40
    • 0344443392 scopus 로고    scopus 로고
    • RNA Degradation in Cell Extracts: Real-Time Monitoring by Fluorescence Resonance Energy Transfer
    • DOI 10.1021/ja036854b
    • Uhler, S.A., Cai, D., Man, Y., Figge, C., and Walter, N.G. (2003). RNA degradation in cell extracts: real-time monitoring by fluorescence resonance energy transfer. J. Am. Chem. Soc. 125, 14230-14231. (Pubitemid 37452334)
    • (2003) Journal of the American Chemical Society , vol.125 , Issue.47 , pp. 14230-14231
    • Uhler, S.A.1    Cai, D.2    Man, Y.3    Figge, C.4    Walter, N.G.5
  • 42
    • 36849023999 scopus 로고    scopus 로고
    • Novel polymerizable surfactants with pH-sensitive amphiphilicity and cell membrane disruption for efficient siRNA delivery
    • DOI 10.1021/bc700285q
    • Wang, X.L., Ramusovic, S., Nguyen, T., and Lu, Z.R. (2007). Novel polymerizable surfactants with pH-sensitive amphiphilicity and cell membrane disruption for efficient siRNA delivery. Bioconjug. Chem. 18, 2169-2177. (Pubitemid 350220022)
    • (2007) Bioconjugate Chemistry , vol.18 , Issue.6 , pp. 2169-2177
    • Wang, X.-L.1    Ramusovic, S.2    Nguyen, T.3    Lu, Z.-R.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.