RNAi in human cells: Basic structural and functional features of small interfering RNA

Molecular Cell

Volumn 10, Issue 3, 2002, Pages 549-561

  Chiu, Ya Lin ^a   Rana, Tariq M ^a  

^a UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOTIN; COMPLEMENTARY RNA; HYDROXYL GROUP; PSORALEN; RNA; RNA DIRECTED RNA POLYMERASE;

ARTICLE; CELL ISOLATION; CONTROLLED STUDY; CROSS LINKING; FEMALE; GENE AMPLIFICATION; HUMAN; HUMAN CELL; IN VIVO STUDY; NONHUMAN; PHOSPHORYLATION; RNA STRUCTURE;

EID: 0036752785     PISSN: 10972765     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1097-2765(02)00652-4     Document Type: Article

References (40)

1. Bass B.L. Double-stranded RNA as a template for gene silencing. Cell. 101:2000;235-238.
2. Bernstein E., Caudy A.A., Hammond S.M., Hannon G.J. Role for a bidentate ribonuclease in the initiation step of RNA interference. Nature. 409:2001;363-366.
3. Cimino G.D., Gamper H.B., Isaacs S.T., Hearst J.E. Psoralens as photoactive probes of nucleic acid structure and function. organic chemistry, photochemistry, and biochemistry Annu. Rev. Biochem. 54:1985;1151-1193.
4. Cogoni C., Macino G. Gene silencing in Neurospora crassa requires a protein homologous to RNA-dependent RNA polymerase. Nature. 399:1999;166-169.
5. Cogoni C., Macino G. Post-transcriptional gene silencing across kingdoms. Curr. Opin. Genet. Dev. 10:2000;638-643.
6. Dalmay T., Hamilton A., Rudd S., Angell S., Baulcombe D.C. An RNA-dependent RNA polymerase gene in Arabidopsis is required for posttranscriptional gene silencing mediated by a transgene but not by a virus. Cell. 101:2000;543-553.
7. Elbashir S.M., Harborth J., Lendeckel W., Yalcin A., Weber K., Tuschl T. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature. 411:2001;494-498. a.
8. Elbashir S.M., Lendeckel W., Tuschl T. RNA interference is mediated by 21- and 22-nucleotide RNAs. Genes Dev. 15:2001;188-200. b.
9. Elbashir S.M., Martinez J., Patkaniowska A., Lendeckel W., Tuschl T. Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysate. EMBO J. 20:2001;6877-6888. c.
10. Fire A., Xu S., Montgomery M.K., Kostas S.A., Driver S.E., Mello C.C. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature. 391:1998;806-811.
11. Grishok A., Tabara H., Mello C.C. Genetic requirements for inheritance of RNAi in C. elegans. Science. 287:2000;2494-2497.
12. Hamilton A.J., Baulcombe D.C. A species of small antisense RNA in posttranscriptional gene silencing in plants. Science. 286:1999;950-952.
13. Hammond S.M., Bernstein E., Beach D., Hannon G.J. An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells. Nature. 404:2000;293-296.
14. Hammond S.M., Caudy A.A., Hannon G.J. Post-transcriptional gene silencing by double-stranded RNA. Nat. Rev. Genet. 2:2001;110-119.
15. Hearst J.E., Isaacs S.T., Kanne D., Rapoport H., Straub K. The reaction of the psoralens with deoxyribonucleic acid. Q. Rev. Biophys. 17:1984;1-44.
16. Kanne D., Straub K., Rapoport H., Hearst J.E. Psoralen-deoxyribonucleic acid photoreaction. Characterization of the monoaddition products from 8-methoxypsoralen and 4,5′8-trimethylpsoralen. Biochemistry. 21:1982;861-871.
17. Kennerdell J.R., Carthew R.W. Use of dsRNA-mediated genetic interference to demonstrate that frizzled and frizzled 2 act in the wingless pathway. Cell. 95:1998;1017-1026.
18. Ketting R.F., Plasterk R.H. A genetic link between co-suppression and RNA interference in C. elegans. Nature. 404:2000;296-298.
19. Lipardi C., Wei Q., Paterson B.M. RNAi as random degradative PCR. siRNA primers convert mRNA into dsRNAs that are degraded to generate new siRNAs Cell. 107:2001;297-307.
20. Lipson S.E., Cimino G.D., Hearst J.E. Structure of M1 RNA as determined by psoralen cross-linking. Biochemistry. 27:1988;570-575.
21. Matzke M., Matzke A.J., Kooter J.M. Rna. guiding gene silencing Science. 293:2001;1080-1083.
22. Mourrain P., Beclin C., Elmayan T., Feuerbach F., Godon C., Morel J.B., Jouette D., Lacombe A.M., Nikic S., Picault N.et al. Arabidopsis SGS2 and SGS3 genes are required for posttranscriptional gene silencing and natural virus resistance. Cell. 101:2000;533-542.
23. Neenhold H.R., Rana T.M. Major groove opening at the HIV-1 Tat binding site of TAR RNA evidenced by a rhodium probe. Biochemistry. 34:1995;6303-6309.
24. Nishikura K. A short primer on RNAi. RNA-directed RNA polymerase acts as a key catalyst Cell. 107:2001;415-418.
25. Nykanen A., Haley B., Zamore P.D. ATP requirements and small interfering RNA structure in the RNA interference pathway. Cell. 107:2001;309-321.
26. Parrish S., Fleenor J., Xu S., Mello C., Fire A. Functional anatomy of a dsRNA trigger. differential requirement for the two trigger strands in RNA interference Mol. Cell. 6:2000;1077-1087.
27. Sharp P.A. RNA interference - 2001. Genes Dev. 15:2001;485-490.
28. Sijen T., Kooter J.M. Post-transcriptional gene-silencing. RNAs on the attack or on the defense? Bioessays. 22:2000;520-531.
29. Sijen T., Fleenor J., Simmer F., Thijssen K.L., Parrish S., Timmons L., Plasterk R.H., Fire A. On the role of RNA amplification in dsRNA-triggered gene silencing. Cell. 107:2001;465-476.
30. Smardon A., Spoerke J.M., Stacey S.C., Klein M.E., Mackin N., Maine E.M. EGO-1 is related to RNA-directed RNA polymerase and functions in germ-line development and RNA interference in C. elegans. Curr. Biol. 10:2000;169-178.
31. Stark G.R., Kerr I.M., Williams B.R., Silverman R.H., Schreiber R.D. How cells respond to interferons. Annu. Rev. Biochem. 67:1998;227-264.
32. Thompson J.F., Hearst J.E. Structure of E. coli 16S RNA elucidated by psoralen crosslinking. Cell. 32:1983;1355-1365.
33. Turner S., Noller H.F. Identification of sites of 4′-(hydroxymethyl)-4,5′,8-trimethoxylpsoralen cross-linking in Escherichia coli 23S ribosomal ribonucleic acid. Biochemistry. 22:1983;4159-4164.
34. Tuschl T. RNA interference and small interfering RNAs. Chembiochem. 2:2001;239-245.
35. Tuschl T., Zamore P.D., Lehmann R., Bartel D.P., Sharp P.A. Targeted mRNA degradation by double-stranded RNA in vitro. Genes Dev. 13:1999;3191-3197.
36. Waterhouse P.M., Wang M.B., Finnegan E.J. Role of short RNAs in gene silencing. Trends Plant Sci. 6:2001;297-301.
37. Weeks K.M., Crothers D.M. RNA recognition by Tat-derived peptides. interaction in the major groove? Cell. 66:1991;577-588.
38. Weeks K.M., Crothers D.M. Major groove accessibility of RNA. Science. 261:1993;1574-1577.
39. Yang D., Lu H., Erickson J.W. Evidence that processed small dsRNAs may mediate sequence-specific mRNA degradation during RNAi in Drosophila embryos. Curr. Biol. 10:2000;1191-1200.
40. Zamore P.D., Tuschl T., Sharp P.A., Bartel D.P. Rnai. double-stranded rna directs the atp-dependent cleavage of mRNA at 21 to 23 nucleotide intervals Cell. 101:2000;25-33.