Effective siRNA delivery and target mRNA degradation using an amphipathic peptide to facilitate pH-dependent endosomal escape

Biochemical Journal

Volumn 435, Issue 2, 2011, Pages 475-487

  Bartz, René ^a   Fan, Haihong ^a   Zhang, Jingtao ^a   Innocent, Nathalie ^a   Cherrin, Craig ^a   Beck, Stephen C ^a   Pei, Yi ^a   Momose, Aaron ^a   Jadhav, Vasant ^a   Tellers, David M ^a   Meng, Fanyu ^a   Crocker, Louis S ^a   Sepp Lorenzino, Laura ^a   Barnett, Stanley F ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

Author keywords

Amphipathic peptide; Endosomal escape; Small interfering RNA (siRNA) delivery; Small interfering RNA (siRNA) trafficking

Indexed keywords

AMPHIPATHIC PEPTIDE; CELL PENETRATING PEPTIDE; HISTIDINE; LEUCINE; MESSENGER RNA; SMALL INTERFERING RNA; UNCLASSIFIED DRUG;

ALPHA HELIX; ARTICLE; CELL ASSAY; CELL FRACTIONATION; CELL MEMBRANE; CONTROLLED STUDY; CYTOSOL; EMBRYO; ENDOCYTOSIS; ENDOSOME; ENDOSOME ESCAPE ASSAY; HUMAN; HUMAN CELL; ISOTHERMAL TITRATION CALORIMETRY; LYSOSOME; NUCLEOTIDE SEQUENCE; PH; PHOSPHATE TRANSPORT; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN DEGRADATION; PROTEIN PROTEIN INTERACTION; PROTEIN SECRETION;

AUTOANTIGENS; BIOLOGICAL TRANSPORT; CELLS, CULTURED; DRUG DELIVERY SYSTEMS; EFFICIENCY; ENDOSOMES; GENE TARGETING; GENE TRANSFER TECHNIQUES; HELA CELLS; HUMANS; HYDROGEN-ION CONCENTRATION; PEPTIDE FRAGMENTS; RIBONUCLEOPROTEINS; RNA INTERFERENCE; RNA STABILITY; RNA, MESSENGER; RNA, SMALL INTERFERING;

EID: 79953232011     PISSN: 02646021     EISSN: 14708728     Source Type: Journal    
DOI: 10.1042/BJ20101021     Document Type: Article

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