The effect of chitosan on transcellular and paracellular mechanisms in the intestinal epithelial barrier

Biomaterials

Volumn 33, Issue 9, 2012, Pages 2791-2800

  Rosenthal, Rita ^a   Günzel, Dorothee ^a   Finger, Caroline ^a   Krug, Susanne M ^a   Richter, Jan F ^a   Schulzke, Jörg Dieter ^b   Fromm, Michael ^a   Amasheh, Salah ^a  

^a HUMBOLDT UNIVERSITY   (Germany)

^b FREIE UNIVERSITÄT BERLIN   (Germany)

Author keywords

Chitin chitosan; Drug delivery; Electrophysiology; Epithelial cell

Indexed keywords

ABSORPTION-ENHANCERS; ACTIN CYTOSKELETON; ACTIN-MYOSIN INTERACTIONS; BARRIER PROPERTIES; CHITIN/CHITOSAN; ENDOCYTOSIS PATHWAY; EPITHELIAL BARRIER; EPITHELIAL CELLS; IMPEDANCE SPECTROSCOPY; INTESTINAL EPITHELIAL CELLS; INTRACELLULAR PH; INTRACELLULAR SIGNALING; ION PERMEABILITY; MAP KINASE; PH-DEPENDENCE; PROTONATED; SUBCELLULAR DISTRIBUTION; TIGHT JUNCTIONS;

CELLS; CHITOSAN; CHLORINE COMPOUNDS; DRUG DELIVERY; ELECTROPHYSIOLOGY; MOLECULAR BIOLOGY; PROTEINS;

PH EFFECTS;

ACTIN; BICARBONATE CHLORIDE ANTIPORTER; CHITOSAN; HEPARIN; MITOGEN ACTIVATED PROTEIN KINASE; PROTEIN KINASE B; SYNAPTOPHYSIN;

ACTIN MYOSIN INTERACTION; ARTICLE; CELL MEMBRANE RESISTANCE; CELL PH; CELLULAR DISTRIBUTION; CONTROLLED STUDY; ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY; ENDOCYTOSIS; HUMAN; HUMAN CELL; INTESTINE EPITHELIUM CELL; INTRACELLULAR SIGNALING; ION PERMEABILITY; PERMEABILITY BARRIER; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTON TRANSPORT; SIGNAL TRANSDUCTION; TIGHT JUNCTION; VASCULAR RESISTANCE;

ACTIN CYTOSKELETON; BIOLOGICAL MARKERS; CELL MEMBRANE PERMEABILITY; CELL SURVIVAL; CHITOSAN; ELECTRIC IMPEDANCE; EPITHELIAL CELLS; HT29 CELLS; HUMANS; INTESTINES; ION TRANSPORT; MEMBRANE PROTEINS; TIGHT JUNCTIONS;

EID: 84855935214     PISSN: 01429612     EISSN: 18785905     Source Type: Journal    
DOI: 10.1016/j.biomaterials.2011.12.034     Document Type: Article

References (45)

1. Valenta C., Auner B.G. The use of polymers for dermal and transdermal delivery. Eur J Pharm Biopharm 2004, 58:279-289.
2. Casettari L., Vllasaliu D., Mantovani G., Howdle S.M., Stolnik S., Illum L. Effect of PEGylation on the toxicity and permeability enhancement of chitosan. Biomacromolecules 2010, 11:2854-2865.
3. Luppi B., Bigucci F., Cerchiara T., Zecchi V. Chitosan-based hydrogels for nasal drug delivery: from inserts to nanoparticles. Expert Opin Drug Deliv 2010, 7:811-828.
4. Alonso M.J., Sanchez A. The potential of chitosan in ocular drug delivery. JPharm Pharmacol 2003, 55:1451-1463.
5. Paolicelli P., de la Fuente M., Sanchez A., Seijo B., Alonso M.J. Chitosan nanoparticles for drug delivery to the eye. Expert Opin Drug Deliv 2009, 6:239-253.
6. Wadhwa S., Paliwal R., Paliwal S.R., Vyas S.P. Chitosan and its role in ocular therapeutics. Mini Rev Med Chem 2009, 9:1639-1647.
7. Davis S.S. Delivery of peptide and non-peptide drugs through the respiratory tract. Pharm Sci Technolo Today 1999, 2:450-456.
8. Thanou M., Verhoef J.C., Junginger H.E. Chitosan and its derivatives as intestinal absorption enhancers. Adv Drug Deliv Rev 2001, 50(Suppl. 1):S91-101.
9. Chopra S., Mahdi S., Kaur J., Iqbal Z., Talegaonkar S., Ahmad F.J. Advances and potential applications of chitosan derivatives as mucoadhesive biomaterials in modern drug delivery. JPharm Pharmacol 2006, 58:1021-1032.
10. Yao J., Zhou J.P., Ping Q.N., Lu Y., Chen L. Distribution of nobiletin chitosan-based microemulsions in brain following i.v. injection in mice. Int J Pharm 2008, 352:256-262.
11. Kotze A.F., Luessen H.L., de Boer A.G., Verhoef J.C., Junginger H.E. Chitosan for enhanced intestinal permeability: prospects for derivatives soluble in neutral and basic environments. Eur J Pharm Sci 1999, 7:145-151.
12. Kotze A.F., Thanou M.M., Luessen H.L., de Boer B.G., Verhoef J.C., Junginger H.E. Effect of the degree of quaternization of N-trimethyl chitosan chloride on the permeability of intestinal epithelial cells (Caco-2). Eur J Pharm Biopharm 1999, 47:269-274.
13. Ranaldi G., Marigliano I., Vespignani I., Perozzi G., Sambuy Y. The effect of chitosan and other polycations on tight junction permeability in the human intestinal Caco-2 cell line(1). JNutr Biochem 2002, 13:157-167.
14. Dorkoosh F.A., Broekhuizen C.A., Borchard G., Rafiee-Tehrani M., Verhoef J.C., Junginger H.E. Transport of octreotide and evaluation of mechanism of opening the paracellular tight junctions using superporous hydrogel polymers in Caco-2 cell monolayers. JPharm Sci 2004, 93:743-752.
15. Smith J., Wood E., Dornish M. Effect of chitosan on epithelial cell tight junctions. Pharm Res 2004, 21:43-49.
16. Opanasopit P., Aumklad P., Kowapradit J., Ngawhiranpat T., Apirakaramwong A., Rojanarata T., et al. Effect of salt forms and molecular weight of chitosans on invitro permeability enhancement in intestinal epithelial cells (Caco-2). Pharm Dev Technol 2007, 12:447-455.
17. Kowapradit J., Opanasopit P., Ngawhirunpat T., Apirakaramwong A., Rojanarata T., Ruktanonchai U., et al. Invitro permeability enhancement in intestinal epithelial cells (Caco-2) monolayer of water soluble quaternary ammonium chitosan derivatives. AAPS PharmSciTech 2010, 11:497-508.
18. Vllasaliu D., Exposito-Harris R., Heras A., Casettari L., Garnett M., Illum L., et al. Tight junction modulation by chitosan nanoparticles: comparison with chitosan solution. Int J Pharm 2010, 400:183-193.
19. Artursson P., Lindmark T., Davis S.S., Illum L. Effect of chitosan on the permeability of monolayers of intestinal epithelial cells (Caco-2). Pharm Res 1994, 11:1358-1361.
20. Schipper N.G., Olsson S., Hoogstraate J.A., deBoer A.G., Varum K.M., Artursson P. Chitosans as absorption enhancers for poorly absorbable drugs 2: mechanism of absorption enhancement. Pharm Res 1997, 14:923-929.
21. Dodane V., Kachar B. Identification of isoforms of G proteins and PKC that colocalize with tight junctions. JMembr Biol 1996, 149:199-209.
22. Dodane V., Amin Khan M., Merwin J.R. Effect of chitosan on epithelial permeability and structure. Int J Pharm 1999, 182:21-32.
23. Smith J.M., Dornish M., Wood E.J. Involvement of protein kinase C in chitosan glutamate-mediated tight junction disruption. Biomaterials 2005, 26:3269-3276.
24. Thanou M., Nihot M.T., Jansen M., Verhoef J.C., Junginger H.E. Mono-N-carboxymethyl chitosan (MCC), a polyampholytic chitosan derivative, enhances the intestinal absorption of low molecular weight heparin across intestinal epithelia invitro and invivo. JPharm Sci 2001, 90:38-46.
25. Sandri G., Bonferoni M.C., Rossi S., Ferrari F., Boselli C., Caramella C. Insulin-loaded nanoparticles based on N-trimethyl chitosan: invitro (Caco-2 model) and exvivo (excised rat jejunum, duodenum, and ileum) evaluation of penetration enhancement properties. AAPS PharmSciTech 2010, 11:362-371.
26. Kudsiova L., Lawrence M.J. Acomparison of the effect of chitosan and chitosan-coated vesicles on monolayer integrity and permeability across Caco-2 and 16HBE14o-cells. JPharm Sci 2008, 97:3998-4010.
27. - secretion in epithelial monolayers of mucus-forming human colon cells (HT-29/B6). Am J Physiol 1991, 261:574-582.
28. Amasheh S., Schmidt T., Mahn M., Florian P., Mankertz J., Tavalali S., et al. Contribution of claudin-5 to barrier properties in tight junctions of epithelial cells. Cell Tissue Res 2005, 321:89-96.
29. Zeissig S., Burgel N., Günzel D., Richter J., Mankertz J., Wahnschaffe U., et al. Changes in expression and distribution of claudin 2, 5 and 8 lead to discontinuous tight junctions and barrier dysfunction in active Crohn's disease. Gut 2007, 56:61-72.
30. + absorption defends from paracellular back-leakage by claudin-8 upregulation. Biochem Biophys Res Commun 2009, 378:45-50.
31. Amasheh M., Fromm A., Krug S.M., Amasheh S., Andres S., Zeitz M., et al. TNFalpha-induced and berberine-antagonized tight junction barrier impairment via tyrosine kinase, Akt and NFkappaB signaling. JCell Sci 2010, 123:4145-4155.
32. Madara J.L., Stafford J. Interferon-gamma directly affects barrier function of cultured intestinal epithelial monolayers. JClin Invest 1989, 83:724-727.
33. Günzel D., Stuiver M., Kausalya P.J., Haisch L., Krug S.M., Rosenthal R., et al. Claudin-10 exists in six alternatively spliced isoforms that exhibit distinct localization and function. JCell Sci 2009, 122:1507-1517.
34. Krug S.M., Fromm M., Günzel D. Two-path impedance spectroscopy for measuring paracellular and transcellular epithelial resistance. Biophys J 2009, 97:2202-2211.
35. Amasheh S., Meiri N., Gitter A.H., Schöneberg T., Mankertz J., Schulzke J.D., et al. Claudin-2 expression induces cation-selective channels in tight junctions of epithelial cells. JCell Sci 2002, 115:4969-4976.
36. Krug S.M., Amasheh S., Richter J.F., Milatz S., Günzel D., Westphal J.K., et al. Tricellulin forms a barrier to macromolecules in tricellular tight junctions without affecting ion permeability. Mol Biol Cell 2009, 20:3713-3724.
37. Markov A.G., Veshnyakova A., Fromm M., Amasheh M., Amasheh S. Segmental expression of claudin proteins correlates with tight junction barrier properties in rat intestine. JComp Physiol B 2010, 180:591-598.
38. Furuse M., Hirase T., Itoh M., Nagafuchi A., Yonemura S., Tsukita S. Occludin: a novel integral membrane protein localizing at tight junctions. JCell Biol 1993, 123:1777-1788.
39. Furuse M., Fujita K., Hiiragi T., Fujimoto K., Tsukita S. Claudin-1 and -2: novel integral membrane proteins localizing at tight junctions with no sequence similarity to occludin. JCell Biol 1998, 141:1539-1550.
40. Ikenouchi J., Furuse M., Furuse K., Sasaki H., Tsukita S. Tricellulin constitutes a novel barrier at tricellular contacts of epithelial cells. JCell Biol 2005, 171:939-945.
41. Yeh T.H., Hsu L.W., Tseng M.T., Lee P.L., Sonjae K., Ho Y.C., et al. Mechanism and consequence of chitosan-mediated reversible epithelial tight junction opening. Biomaterials 2011, 32:6164-6173.
42. Kotze A.F., Luessen H.L., de Leeuw B.J., de Boer A.G., Verhoef J.C., Junginger H.E. Comparison of the effect of different chitosan salts and N-trimethyl chitosan chloride on the permeability of intestinal epithelial cells (Caco-2). JControl Release 1998, 51:35-46.
43. Hamman J.H., Schultz C.M., Kotze A.F. N-trimethyl chitosan chloride: optimum degree of quaternization for drug absorption enhancement across epithelial cells. Drug Dev Ind Pharm 2003, 29:161-172.
44. McEwan G.T., Jepson M.A., Hirst B.H., Simmons N.L. Polycation-induced enhancement of epithelial paracellular permeability is independent of tight junctional characteristics. Biochim Biophys Acta 1993, 1148:51-60.
45. Escaffit F., Boudreau F., Beaulieu J.F. Differential expression of claudin-2 along the human intestine: implication of GATA-4 in the maintenance of claudin-2 in differentiating cells. JCell Physiol 2005, 203:15-26.