Production and characterization of recombinant transmembrane proteins from Mycoplasma hyopneumoniae

Veterinary Microbiology

Volumn 155, Issue 1, 2012, Pages 44-52

  Marchioro, S B ^a   Simionatto, S ^a,b   Galli, V ^a   Conceicao F R ^a   Brum, C B ^a   Fisch, A ^a   Gomes, C K ^a   Dellagostin, O A ^a  

^a FEDERAL UNIVERSITY OF PELOTAS   (Brazil)

^b UNIVERSIDADE FEDERAL DA GRANDE DOURADOS   (Brazil)

Author keywords

Mycoplasma hyopneumoniae; Swine enzootic pneumonia; Transmembrane proteins

Indexed keywords

BACTERIAL VACCINE; BACTERIN; MEMBRANE PROTEIN; RECOMBINANT ABC TRANSPORTER PROTEIN; RECOMBINANT CARRIER PROTEIN; RECOMBINANT HEMOLYSIN C; RECOMBINANT HYPOTHETICAL PROTEIN; RECOMBINANT MAGNESIUM TRANSPORT PROTEIN; RECOMBINANT PUTATIVE INTEGRATIVE CONJUGAL ELEMENT 2 PROTEIN; RECOMBINANT SERINE PROTEASE; RECOMBINANT TRANSMEMBRANE PROTEIN; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIGENICITY; ARTICLE; CONTROLLED STUDY; DRUG SCREENING; DRUG SYNTHESIS; ESCHERICHIA COLI; IMMUNOGENICITY; MOUSE; MYCOPLASMA HYOPNEUMONIAE; MYCOPLASMAL PNEUMONIA OF SWINE; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION;

ANIMALS; ANTIBODIES, BACTERIAL; ANTIGENS, BACTERIAL; ESCHERICHIA COLI; FEMALE; IMMUNITY, HUMORAL; MALE; MEMBRANE PROTEINS; MICE; MYCOPLASMA HYOPNEUMONIAE; RECOMBINANT PROTEINS; VACCINES, SYNTHETIC;

ESCHERICHIA COLI; MUS; MYCOPLASMA HYOPNEUMONIAE; SUIDAE;

EID: 84855884275     PISSN: 03781135     EISSN: 18732542     Source Type: Journal    
DOI: 10.1016/j.vetmic.2011.08.001     Document Type: Article

References (38)

1. Adu-Bobie J., Capecchi B., Serruto D., Rappuoli R., Pizza M. Two years into reverse vaccinology. Vaccine 2003, 21:605-610.
2. Brusic V., Bucci K., Schonbach C., Petrovsky N., Zeleznikow J., Kazura J.W. Efficient discovery of immune response targets by cyclical refinement of QSAR models of peptide binding. J. Mol. Graph. Model. 2001, 19:405-411.
3. Castro L.A., Pedroso T.R., Kuchiishi S.S., Ramenzoni M., Kich J.D., Zaha A., Vainstein M.H., Ferreira H.B. Variable number of tandem aminoacid repeats in adhesion-related CDS products in Mycoplasma hyopneumoniae strains. Vet. Microbiol. 2006, 116:258-269.
4. Chen J.R., Liao C.W., Mao S.J., Weng C.N. A recombinant chimera composed of repeat region RR1 of Mycoplasma hyopneumoniae adhesin with Pseudomonas exotoxin: in vivo evolution of specific IgG response in mice and pigs. Vet. Microbiol. 2001, 80:347-357.
5. Chen Y.L., Wang S.N., Yang W.J., Chen Y.J., Lin H.H., Shiuan D. Expression and immunogenicity of Mycoplasma hyopneumoniae heat shock protein antigen P42 by DNA vaccination. Infect. Immun. 2003, 71:1155-1160.
6. Chen A.Y., Fry S.R., Forbes-Faulkner J., Daggard G., Mukkur T.K. Evaluation of the immunogenicity of the P97R1 adhesin of Mycoplasma hyopneumoniae as a mucosal vaccine in mice. J. Med. Microbiol. 2006, 55:923-929.
7. Chen A.Y., Fry S.R., Daggard G.E., Mukkur T.K.S. Evaluation of immune response to recombinant potential protective antigens of Mycoplasma hyopneumoniae delivered as cocktail DNA and/or recombinant protein vaccines in mice. Vaccine 2008, 26:4372-4378.
8. Conceição F.R., Moreira A.N., Dellagostin O.A. A recombinant chimera composed of R1 repeat region of Mycoplasma hyopneumoniae P97 adhesin with Escherichia coli heat-labile enterotoxin B subunit elicits immune response in mice. Vaccine 2006, 24:5734-5743.
9. De Groot A.S. Immunomics: discovering new targets for vaccine and therapeutics. Drug Discov. Today 2006, 11:203-209.
10. De Lalla C., Sturniolo T., Abbruzzese L., Hammer J., Sidoli A., Sinigaglia F., Panina-Bordignon P. Cutting edge: identification of novel T cell epitopes in Lol p5a by computational prediction. J. Immunol. 1999, 163:1725-1729.
11. Depilet S., Morales O., Castelli F.A., Delhem N., Francois V., Georges B., Dufosse F., Morschhauser F., Hammer J., Maillere B. Determination of a HLA II promiscuous peptide cocktail as potential vaccine against EBV latency II malignancies. J. Immunother. 2007, 30:215-226.
12. Djordjevic S.P., Eamens G.J., Romalis L.F., Nicholis P.J., Taylor V., Chin J. Serum and mucosal antibody responses and protection in pigs vaccinated against Mycoplasma hyopneumoniae with vaccines containing a denatured membrane antigen pool and adjuvant. Aust. Vet. J. 1997, 75:504-511.
13. Fagan P.K., Djordjevic S.P., Eamens G.J., Chin J., Walker M.J. Molecular characterization of a ribonucleotideredutase (nrdF) gene fragment of Mycoplasma hyopneumoniae and assessment of the recombinant product as an experimental vaccine for enzootic pneumonia. Infect. Immun. 1996, 64:1060-1064.
14. Georgiou G., Segatori L. Preparative expression of secreted proteins in bacteria: status report and future prospects. Curr. Opin. Biotechnol. 2005, 16:538-545.
15. Haesebrouck F., Pasmans F., Chiers K., Maes D., Ducatelle R., Decostere A. Efficacy of vaccines against bacterial diseases in swine: what can we expect?. Vet. Microbiol. 2004, 100:255-268.
16. Hakenberg J., Nussbaum A.K., Schild H., Rammensee H.G., Kuttler C., Holzhütter H.G., Kloetzel P.M., Kaufmann S.H., Mollenkopf H.J. MAPPP: MHC class I antigenic peptide processing prediction. Appl. Bioinform. 2003, 2:155-158.
17. Honeyman M.C., Brusic V., Stone N.L., Harrison L.C. Neural network-based prediction of candidate T cell epitopes. Nat. Biotechnol. 1998, 16:966-969.
18. Janulczyk R., Rasmussen M. Improved pattern for genome-based screening identifies novel cell wall-attached proteins in gram-positive bacteria. Infect. Immun. 2001, 69:4019-4026.
19. Lin J., Weng C.N., Liao C.W., Yeh K.S., Pan M.J. Protective effects of oral microencapsulated Mycoplasma hyopneumoniae vaccine prepared by co-spray drying method. J. Vet. Med. Sci. 2003, 65:69-74.
20. Liu W., Feng Z., Fang L., Zhou Z., Qiang L., Li S., Luo R., Wang L., Chen H., Shao G., Xiao S. Complete genome sequence of Mycoplasma hyopneumoniae strain 168. J. Bacteriol. 2011, 193:1016-1017.
21. Maes D., Segales J., Meyns T., Sibila M., Pieters M., Haesebrouck F. Control of Mycoplasma hyopneumoniae infections in pigs. Vet. Microbiol. 2008, 126:297-309.
22. Meens J., Bolotin V., Frank R., Böhmer J., Gerlach G.F. Characterization of a highly immunogenic Mycoplasma hyopneumoniae lipoprotein Mhp366 identified by peptide-spot array. Vet. Microbiol. 2009, 142:293-302.
23. Meyns T., Dewulf J., de K.A., Calus D., Haesebrouck F., Maes D. Comparison of transmission of Mycoplasma hyopneumoniae in vaccinated and non-vaccinated populations. Vaccine 2006, 24:7081-7086.
24. Minion F.C., Lefkowitz E.J., Madsen M.L., Cleary B.J., Swartzell S.M., Mahairas G.G. The genome sequence of Mycoplasma hyopneumoniae strain 232, the agent of swine mycoplasmosis. J. Bacteriol. 2004, 186:7123-7133.
25. Okamba F.R., Arellaa M., Musicb N., Jia J.J., Gottschalkb M., Gagnonb C.A. Potential use of a recombinant replication-defective adenovirus vector carrying the C-terminal portion of the P97 adhesin protein as a vaccine against Mycoplasma hyopneumoniae in swine. Vaccine 2010, 28:4802-4809.
26. Rappuoli R. Reverse vaccinology. Curr. Opin. Microbiol. 2000, 3:445-450.
27. Reynolds S.C., St Aubin L.B., Sabbadini L.G., Kula J., Vogelaar J., Runnels P., Peters A.R. Reduced lung lesions in pigs challenged 25 weeks after the administration of a single dose of Mycoplasma hyopneumoniae vaccine at approximately 1 week of age. Vet. J. 2009, 181:312-320.
28. Sambrook J., Russell D.W. Molecular Cloning: A Laboratory Manual 2001, Cold Spring Harbor Laboratory Press, New York. 3rd ed.
29. Shimoji Y., Oishi E., Muneta Y., Nosaka H., Mori Y. Vaccine efficacy of the attenuated Erysipelothrix rhusiopathiae YS-19 expressing a recombinant protein of Mycoplasma hyopneumoniae P97 adhesin against mycoplasmal pneumonia of swine. Vaccine 2003, 21:532-537.
30. Simionatto S., Marchioro S.B., Galli V., Luerce T.D., Hartwig D.D., Moreira A.N., Dellagostin O.A. Efficient site-direct mutagenesis using an overlap extension-PCR method for expressing Mycoplasma hyopneumoniae genes in Escherichia coli. J. Microbiol. Meth. 2009, 79:101-105.
31. Simionatto S., Marchioro S.B., Galli V., Hartwig D.D., Carlessi R.M., Munari F.M., Laurino J.P., Conceição F.R., Dellagostin O.A. Cloning and purification of recombinant proteins of Mycoplasma hyopneumoniae expressed in Escherichia coli. Protein Expr. Purif. 2010, 69:132-136.
32. Thacker E.L., Thacker B.J., Boettcher T.B., Jayappa H. Comparison of antibody production, lymphocyte stimulation, and protection induced by four commercial Mycoplasma hyopneumoniae bacterins. J. Swine Health Prod. 1998, 6:107-112.
33. Thacker E., Thacker B.J., Kuhn M., Hawkins P.A., Waters W.R. Evaluation of local and systemic immune responses induced by intramuscular injection of a Mycoplasma hyopneumoniae bacterin to pigs. Am. J. Vet. Res. 2000, 61:1384-1389.
34. Thacker E.L. Mycoplasmal diseases. Diseases of Swine 2006, 701-717. Blacwell Publishing Ltd., Oxford, UK. 9th ed. B.E. STRAW, J.J. Zimmerman, S. D'Allaire, D.J. Taylor (Eds.).
35. Vasconcelos A.T., Ferreira H.B., Bizarro C.V., Bonatto S.L., Carvalho M.O., Pinto P.M., Almeida D.F., Almeida L.G., Almeida R., Alves-Filho L., Assunção E.N., Azevedo V.A., Bogo M.R., Brigido M.M., Brocchi M., Burity H.A., Camargo A.A., Camargo S.S., Carepo M.S., Carraro D.M., de Mattos Cascardo J.C., Castro L.A., Cavalcanti G., Chemale G., Collevatti R.G., Cunha C.W., Dallagiovanna B., Dambros B.P., Dellagostin O.A., Falcao C., Fantinatti-Garboggini F., Felipe M.S., Fiorentin L., Franco G.R., Freitas N.S., Frias D., Grangeiro T.B., Grisard E.C., Guimarães C.T., Hungria M., Jardim S.N., Krieger M.A., Laurino J.P., Lima L.F., Lopes M.I., Loreto E.L., Madeira H.M., Manfio G.P., Maranhão A.Q., Martinkovics C.T., Medeiros S.R., Moreira M.A., Neiva M., Ramalho-Neto C.E., Nicolas M.F., Oliveira S.C., Paixao R.F., Pedrosa F.O., Pena S.D., Pereira M., Pereira-Ferrari L., Piffer I., Pinto L.S., Potrich D.P., Salim A.C., Santos F.R., Schmitt R., Schneider M.P., Schrank A., Schrank I.S., Schuck A.F., Seuanez H.N., Silva D.W., Silva R., Silva S.C., Soares C.M., Souza K.R., Souza R.C., Staats C.C., Steffens M.B., Teixeira S.M., Urmenyi T.P., Vainstein M.H., Zuccherato L.W., Simpson A.J., Zaha A. Swine, poultry pathogens: the complete genome sequences of two strains of Mycoplasma hyopneumoniae, a strain of Mycoplasma synoviae. J. Bacteriol. 2005, 187:5568-5577.
36. Wagner S., Baars L., Ytterberg A.J., Klussmeier A., Wagner C.S., Nord O., Nygren P.A., van Wijk K.J., de Gier J.W. Consequences of membrane protein overexpression in Escherichia coli. Mol. Cell. Proteomics 2007, 6:1527-1550.
37. Yang W.J., Lai J.F., Peng K.C., Chiang H.J., Weng C.N., Shiuan D. Epitope mapping of Mycoplasma hyopneumoniae using phage displayed peptide libraries and the immune responses of the selected phagotopes. J. Immunol. Methods 2005, 304:15-29.
38. Yoshino T., Shimojo A., Maeda Y., Matsunaga T. Inducible expression of transmembrane proteins on bacterial magnetic particles in MagnetospirillummagneticumAMB-1. Appl. Environ. Microbiol. 2010, 76:1152-1157.