Molecular characterization of a ribonucleotide reductase (nrdF) gene fragment of Mycoplasma hyopneumoniae and assessment of the recombinant product as an experimental vaccine for enzootic pneumonia

Infection and Immunity

Volumn 64, Issue 3, 1996, Pages 1060-1064

  Fagan, Peter K ^a,b   Djordjevic, Steven P ^b   Eamens, Graeme J ^b   Chin, James ^b   Walker, Mark J ^a  

^a UNIVERSITY OF WOLLONGONG   (Australia)

^b NSW DEPARTMENT OF PRIMARY INDUSTRIES   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; HYBRID PROTEIN; RECOMBINANT VACCINE; RIBONUCLEOTIDE REDUCTASE;

ANIMAL DISEASE; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BACTERIAL GENE; BACTERIUM ISOLATION; CONTROLLED STUDY; ENZYME SUBUNIT; GRAM NEGATIVE INFECTION; MOLECULAR CLONING; MYCOPLASMA; NONHUMAN; PNEUMONIA; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; SWINE DISEASE;

EID: 0030020421     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/iai.64.3.1060-1064.1996     Document Type: Article

References (40)

1. Burnette, W. N. 1981. Western blotting: electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal. Biochem. 112:195-203.
2. Ciprian, A., C. Pijoan, T. Cruz, J. Camacho, J. Tórtora, G. Colmenares, R. López-Revilla, and M. de la Garza. 1988. Mycoplasma hyopneumoniae increases the susceptibility of pigs to experimental Pasteurella multocida pneumonia. Can. J. Vet. Res. 52:434-438.
3. Cooper, P. D., and E. J. Steele. 1991. Algammulin, a new vaccine adjuvant comprising gamma inulin particles containing alum preparation and in vitro properties. Vaccine 9:351-357.
4. Dattananda, C. S., K. Rajkumari, and J. Gowrishankar. 1991. Multiple mechanisms contribute to osmotic inducibility of proU operon expression in Escherichia coli: demonstration of two osmoresponsive promoters and of a negative regulatory element within the first structural gene. J. Bacteriol. 173:7481-7490
5. Dayalu, K. I., R. L. Keich, P. Charlier, and S. Martinod. 1992. Evaluation of the beneficial effects of a Mycoplasma hyopneumoniae vaccine (Respisure): results from controlled and field studies, p. 302. In Proceedings of the 12th Congress of the International Pig Veterinary Society Royal Netherlands Veterinary Association, Boxtel, The Netherlands.
6. Djordjevic, S. P., G. J. Eamens, L. F. Romalis, and J. C. Chin. Unpublished data.
7. Etheridge, J. R., G. S. Cottew, and L. C. Lloyd. 1979. Isolation of Mycoplasma hyopneumoniae from lesions in experimentally infected pigs. Aust. Vet. J 55:356-359.
8. Falk, K., S. Hoie, and B. M. Lium. 1991. An abattoir survey of pneumonia and pleuritis in slaughter weight from 9 selected herds. II. Enzootic pneumonia of pigs: microbiological findings and their relationship to pathomorphology Acta Vet. Scand. 32:67-77.
9. Gilson, E., G. Allong, T. Schmidt, J. Claverys, R. Dudler, and M. Hofnung. 1988. Evidence for high affinity binding protein dependent transport systems in Gram-positive bacteria and in Mycoplasma. EMBO J 7:3971-3974.
10. Goodwin, R. F. W., R. G. Hodgson, P. Whittlestone, and R. L. Woodhams. 1969. Some experiments relating to artificial immunity in enzootic pneumonia in pigs. J Hyg 67:193-208
11. Gowrishankar, J. 1989. Nucleotide sequence of the osmoregulatory proU operon of Escherichia coli. J. Bacteriol. 171:1923-1931.
12. Harlow, E., and D. Lane. 1988. Antibodies: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
13. Herrmann, R. 1992 Genome structure and organization, p. 157-168. In J. Maniloff, R. N. McElhaney, L. R. Finch, and J. B. Baseman (ed.), Mycoplasmas: molecular biology and pathogenesis. American Society for Microbiology. Washington, D.C
14. Hoskinson, R. M., R. D. G. Rigby, P. E. Mattner, V. L. Iluyhn, M. D'Occhio, A. Neish, T. E. Trigg, B. A. Moss, M. J. Lindsey, G. D. Coleman, and C. L. Schwartzkoff. 1990. Vaxtrate: an anti-reproductive vaccine for cattle. Aust. J. Biotechnol. 4:166-176.
15. Howell, M. J., and J. J. Hargreaves. 1988. Cloning and expression of Taenia ovis antigens in Escherichia coli. Mol. Biochem Parasitol. 28:21-30.
16. Huhn, R. G. 1970. Swine enzootic pneumonia: incidence and effect on rate of body weight gain. Am. J Vet Res 31:1097-1108.
17. Jordan, A., I. Gibert, and J. Barbe. 1994. Cloning and sequencing of the genes from Salmonella typhimurium encoding a new bacterial ribonucleotide reductase. J. Bacteriol. 176:3420-3427
18. Kobisch, M., B. Blanchard, and M. F. Le Potier. 1993. Mycoplasma hyopneumoniae infection in pigs: duration of the disease and resistance to reinfection. Vet Res. 24:67-77
19. Kristensen, B., P. Poroz, J. Nicolet, M. Wanner, and A. L. deWeck. 1981. Cell-mediated and humoral response in swine after vaccination and natural infection with Mycoplasma hyopneumoniae. Am J. Vet. Res. 42:784-788.
20. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680-685.
21. Lucht, J. M., and E. Bremer. 1991. Characterization of mutations affecting the osmoregulated proU promoter of Escherichia coli and identification of 5′ sequences required for high-level expression. J. Bacteriol. 173:801-809.
22. Meissner, P. S., W. P. Sisk, and M. L. Berman. 1987. Bacteriophage λ cloning system for the construction of directional cDNA libraries. Proc Natl. Acad. Sci. USA 84:4171.
23. Miller, S. K., R. F. Ross, B. Z. Erickson, D. Gerber, R. H. Schultz, and D. W. Chladek. 1992. Response of pigs vaccinated with Mycoplasma hyopneumoniae vaccines to challenge with Mycoplasma hyopneumomae, p. 324. In Proceedings of the 12th Congress of the International Pig Veterinary Society. Royal Netherlands Veterinary Association, Boxtel, The Netherlands
24. Morrison, R. B., C. Pijoan, and A. D. Leman. 1986. Association between enzootic pneumonia and performance. Pig News Inform. 7:23-31.
25. Murphy, D. A., W. G. Van Alstine, L. K. Clark, S. Albregts, and K. Knox. 1993. Aerosol vaccination of pigs against Mycoplasma hyopneumoniae infection Am J. Vet. Res. 34:1874-1880.
26. Peterson, S. N., P.-C. Hu, K. F. Bott, and C. A. Hutchinson III. 1993. A survey of the Mycoplasma genitalium genome by using random sequencing. J. Bacteriol. 175:7918-7930
27. Pointon, A. M., D. Byrt, and P. Heap. 1985. Effect of enzootic pneumonia of pigs on growth performance. Aust. Vet. J. 62:13-18.
28. Razin, S., J. G. Tully, D. L. Rose, and M. F. Barile. 1983 DNA cleavage patterns as indicators of genetic heterogeneity among strains of Acholeplasma and Mycoplasma species. J. Gen. Microbiol. 129:1935-1944
29. Reichard, P. 1993. From RNA to DNA, why so many reductases? Science 260:1773-1777.
30. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press. Cold Spring Harbor, N Y.
31. Sanger, F., S. Nicklen, and A. R. Coulsen. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
32. Sheldrake, R. F., and L. F. Romalis. 1992. Evaluation of an enzyme-linked immunosorbant assay for the detection of Mycoplasma hyopneumoniae antibody in porcine serum. Aust. Vet. J. 69:255-259.
33. Simecka, J. W., J. K. Davis, M. K. Davidson, S. E. Ross, C. T. K.-H. Städtlander, and G. H. Cassell. 1992. Mycoplasma diseases of animals, p. 391-415. In J. Maniloff, R. N. McElhaney, L R. Finch, and J. B. Baseman (ed.), Mycoplasmas: molecular biology and pathogenesis. American Society for Microbiology, Washington, D C
34. Southern, E. M. 1975 Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98:503-517.
35. Stanley, K. K., and J. P. Luzio. 1984. Construction of a new family of high efficiency bacterial expression vectors: identification of cDNA clones coding for human liver proteins. EMBO J. 3:1429-1434.
36. Takeshita, S., M. Sato, M. Toba, W. Masahashi, and T. Hashimoto-Gotoh. 1987. High-copy-number and low-copy-number plasmid vectors for LacZ a-complementation and chloramphenicol or kanamycin-resistance selection. Gene 61:63-74.
37. Taschke, C., M.-Q. Klinkert, E. Pirkl, and R. Herrmann. 1987 Gene expression signals in Mycoplasma hyopneumoniae and Mycoplasma capricolum. Isr. J. Med. Sci. 23:347-351.
38. Thelander, L., and P. Reichard. 1979 Reduction of ribonucleotides. Annu Rev. Biochem. 48:133-158
39. Yagihashi, T., T. Nunoya, T. Mitui, and M. Tajima. 1984. Effect of Mycoplasma hyopneumoniae infection on the development of Haemophilus pleuropneumoniae pneumonia in pigs Jpn. J. Vet. Sci. 46:705-713.
40. Yogev, D., R. Rosengarten, R. Watson-McKown, and K. S. Wise, 1991 Molecular basis of Mycoplasma surface variation: a novel set of divergent genes undergo spontaneous mutation of periodic coding regions and 5′ regulatory sequences. EMBO J. 10:4069-4079.