High expression of recombinant Streptomyces sp. S38 xylanase in Pichia pastoris by codon optimization and analysis of its biochemical properties

Molecular Biology Reports

Volumn 38, Issue 8, 2011, Pages 4991-4997

  Fu, Xiao Yan ^a   Zhao, Wei ^a   Xiong, Ai Sheng ^a   Tian, Yong Sheng ^a   Peng, Ri He ^a  

^a INSTITUTE OF EDIBLE FUNGI   (China)

Author keywords

Codon optimization; Expression; Pichia pastoris; Streptomyces sp. S38 xylanase

Indexed keywords

ALUMINUM; CADMIUM; CALCIUM ION; CHROMIUM; CUPRIC ION; FERROUS ION; LEAD; MAGNESIUM ION; MANGANESE; MERCURY; POTASSIUM ION; XYLAN ENDO 1,3 BETA XYLOSIDASE; ZINC ION;

ALCOHOL OXIDASE 1 GENE; ARTICLE; BACTERIAL GENE; CODON OPTIMIZATION; CODON USAGE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME INHIBITION; ENZYME STABILITY; EXPRESSION VECTOR; GENE; GENE EXPRESSION REGULATION; GENE SYNTHESIS; GENETIC ANALYSIS; GENETIC TRANSFORMATION; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROMOTER REGION; RECOMBINANT STREPTOMYCES XYLANASE GENE; STREPTOMYCES; TEMPERATURE;

CLONING, MOLECULAR; CODON; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDO-1,4-BETA XYLANASES; ENZYME STABILITY; GENE EXPRESSION; GENETIC VECTORS; GLYCOSYLATION; HYDROGEN-ION CONCENTRATION; IONS; KINETICS; METALS; MOLECULAR BIOLOGY; PICHIA; RECOMBINANT PROTEINS; RECOMBINATION, GENETIC; STREPTOMYCES; TEMPERATURE;

PICHIA PASTORIS; STREPTOMYCES SP.;

EID: 84855227216     PISSN: 03014851     EISSN: 15734978     Source Type: Journal    
DOI: 10.1007/s11033-010-0644-7     Document Type: Article

References (39)

1. Singh S, Madlala AM, Prior BA (2003) Thermomyces lanugi-nosus: properties of strains and their hemicellulases. FEMS Microbiol Rev 27:3-16 (Pubitemid 36412184)
2. Polizeli MLTM, Rizzatti ACS, Monti R, Terenzi HF, Jorge JA, Amorim DS (2005) Xylanases from fungi: properties and industrial applications. Appl Microbiol Biotechnol 67:577-591 (Pubitemid 40903124)
3. Biely P (1985) Microbial xylanolytic systems. Trends Biotechnol 3:286-290 (Pubitemid 16188081)
4. Silversides FG, Bedford MR (1999) Effect of pelleting temperature on the recovery and efficacy of a xylanase enzyme in wheat-based diets. Poultry Sci 78:1184-1190 (Pubitemid 129555935)
5. Kung L Jr, Treacher RJ, Nauman GA, Smagala AM, Endres KM, Cohen MA (2000) The effect of treating forages with fibrolytic enzymes on its nutritive value and lactation performance of dairy cows. J Dairy Sci 83:115-122
6. Kuhad RC, Singh A (1993) Lignocellulose biotechnology: current and future prospects. Crit Rev Biotechnol 13:151-172
7. Figueroa-Espinoza MC, Poulsen C, Borch Soe J, Zargahi MR, Rouau X (2004) Enzymatic solubilization of arabinoxylans from native, extruded, and high-shear-treated rye bran by different endo-xylanases and other hydrolyzing enzymes. J Agric Food Chem 52:4240-4249
8. Hang YD, Woodams EE (1997) Xylanolytic activity of commercial juice-processing enzyme preparations. Lett Appl Micro-biol 24:389-392 (Pubitemid 27239558)
9. Subramaniyan S, Prema P (2002) Biotechnology of microbial xylanases: enzymology, molecular biology, and application. Crit Rev Biotechnol 22:33-64 (Pubitemid 34272292)
10. Wong KKY, Saddler JN (1992) Trichoderma xylanases, their properties and application. Crit Rev Biotechnol 12:413-435
11. Sunna A, Antranikian G (1997) Xylanolytic enzymes from fungi and bacteria. Crit Rev Biotechnol 17:39-67 (Pubitemid 127716370)
12. Wong KKY, Tan LU, Saddler JN (1988) Multiplicity of b-1, 4-xylanase in microorganisms: functions and applications. Micro-biol Rev 52:305-317
13. Gilkes NR, Henrissat B, Kilburn DG, Miller RC Jr, Warren RA (1991) Domains in microbial beta-1,4-glycanases: sequence conservation, function, and enzyme families. Microbiol Rev 55:303-315 (Pubitemid 21895156)
14. Henrissat B, Bairoch A (1993) New families in the classification of glycosyl hydrolases based on amino acid sequence similarities. Biochemi J 293:781-788 (Pubitemid 23244564)
15. Henrissat B, Davies G (1997) Structural and sequence-based classification of glycoside hydrolases. Curr Opin Struc Biol 7:637-644 (Pubitemid 27472582)
16. Garg AP, Roberts JC, McCarthy AJ (1998) Bleachboosting effect of cellulase-free xylanase of Streptomyces thermoviolaceus and its comparison with two commercial enzyme preparations on birchwood kraft pulp. Enzyme Microb Technol 22:594-598 (Pubitemid 28260575)
17. Cereghino JL, Cregg JM (2000) Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev 24:45-66 (Pubitemid 30047114)
18. Xiong AS, Yao QH, Peng RH, Li X, Fan HQ, Li Y, Cheng ZM (2004) A simple, rapid, high fidelity and cost-effective PCR based two-step DNA synthesis (PTDS) method for long gene sequences. Nucleic Acids Res 32:e98
19. Xiong AS, Peng RH, Li X, Fan HQ, Yao QH, Guo MJ, Zhang SL (2003) Influence of signal peptide sequences on the expression of heterogeneous proteins in Pichia pastoris. Acta Biochim Biophys Sin 35:154-160 (Pubitemid 36419134)
20. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor, New York
21. Bailey MJ, Biely P, Poutanen K (1992) Interlaboratory testing of methods for assay of xylanase activity. J Biotechnol 32:257-270
22. Bhat MK (2000) Cellulases and related enzymes in biotechnology. Biotechnol Adv 18:355-383
23. Aristidou A, Pentillä M (2000) Metabolic engineering applications to renewable resource utilization. Curr Opin Biotechnol 11:187-198 (Pubitemid 30182315)
24. Beg QK, Bhushan B, Kapoor M, Hoondal GS (2000) Enhanced production of a thermostable xylanase from Streptomyces sp. QG-11-3 and its application in biobleaching of eucalyptus kraft pulp. Enzyme Microb Technol 27:459-466
25. Beg QK, Kapoor M, Mahajan L, Hoondal GS (2001) Microbial xylanases and their industrial applications: a review. Appl Microbiol Biotechnol 56:326-338 (Pubitemid 32763342)
26. Techapun C, Poosaran N, Watanabe M, Sasaki K (2003) Thermostable and alkaline-tolerant microbial cellulose-free xylanases produced from agricultural wastes and the properties required for use in pulp bleaching bioprocesses: a review. Process Biochem 38:1327-1340 (Pubitemid 36708616)
27. Berrin JG, Williamson G, Puigserver A, Chaix JC, Mclauchlan WR, Juge N (2000) High-level production of recombinant fungal endo-b-1,4-xylanase in the methylotrophic yeast Pichia pastoris. Protein Expr Purif 19:179-187 (Pubitemid 30387909)
28. Zhang HL, Yao B, Wang YR, Zhang WZ (2003) Expression of xylanase gene xynA from Streptomyces olivaceoviridis A1 in Escherichia coli and Pichia pastoris. Chin J Biotechnol 19:41-45
29. Jiang ZB, Song HT, Ma LX (2003) Secreted expression of Bacillus pumilus xylanase gene in Pichia pastoris and study on enzymatic properties. Chin J Biotechnol 19:50-55
30. Damaso MCT, Almeida MS, Kurtenbach E, Martins OBNP Jr, Andrade CMMC, Albano RM (2003) Optimized expression of a thermostable xylanase from Thermomyces lanuginosus in Pichia pastoris. Appl Environ Microbiol 69:6064-6072 (Pubitemid 37287580)
31. Tanaka H, Okuno T, Moriyama S, Muguruma M, Ohta K (2004) Acidophilic xylanase from Aureobasidum pullulans: efficient expression and secretion in Pichia pastoris and mutational analysis. J Biosci Bioeng 98:338-343 (Pubitemid 40013198)
32. Chantasingh D, Pootanakit K, Champreda V, Kanokratana P, Eurwilaichitr L (2006) Cloning, expression, and characterization of a xylanase 10 from Aspergillus terreus (BCC129) in Pichia pastoris. Protein Expr Purif 46:143-149 (Pubitemid 43247007)
33. Cheng YF, Yang CH, Liu WH (2005) Cloning and expression of Thermobifida xylanase gene in the methylotrophic yeast Pichia pastoris. Enzyme Microb Technol 37:541-546
34. Ramchurn SO, Mateus B, Holst O, Karlsson EN (2005) The methylotrophic yeast pichia pastoris as a host for the expression and production of thermostable xylanase. FEMS Yeast Res 5: 839-850 (Pubitemid 40733230)
35. Georis J, Giannotta F, Buyl ED, Granier B, Frère JM (2000) Purification and properties of three ento-b-1,4-xylanases produced by Streptomyces sp.strain S38 which differ in their ability to enhance the bleaching of kraft pulps. Enzyme Microb Technol 26:178-186
36. Zhou C, Bai J, Deng S, Wang J, Zhu J, Wu M, Wang W (2008) Cloning of a xylanase gene from Aspergillus usamii and its expression in Escherichia coli. Bioresour Technol 99:831-838 (Pubitemid 350088445)
37. Zhang M, Jiang Z, Yang S, Hua C, Li L (2010) Bioresource technology cloning and expression of a Paecilomyces thermo-phila xylanase gene in E. coli and characterization of the recombinant xylanase. Bioresour Technol 101(2):688-695
38. Ghaffar A, Khan SA, Mukhtar Z, Rajoka MI, Latif F. (2010) Heterologous expression of a gene for thermostable xylanase from Chaetomium thermophilum in Pichia pastoris GS115. Mol Biol Rep. http://www.ncbi.nlm.nih.gov/pubmed/20213504
39. He J, Yu B, Zhang K, Ding X, Chen D (2009) Expression of endo-1,4-beta-xylanase from Trichoderma reesei in Pichia pas-toris and functional characterization of the produced enzyme. BMC Biotechnol 10:1-10