Expression of endo-1, 4-beta-xylanase from Trichoderma reesei in Pichia pastoris and functional characterization of the produced enzyme

BMC Biotechnology

Volumn 9, Issue , 2009, Pages

  He, Jun ^a,b   Yu, Bing ^a,b   Zhang, Keying ^a,b   Ding, Xuemei ^a,b   Chen, Daiwen ^a,b  

^a SICHUAN AGRICULTURAL UNIVERSITY   (China)

^b BEIJING UNIVERSITY OF POSTS AND TELECOMMUNICATIONS   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ALCOHOL OXIDASE; CULTURE SUPERNATANT; ENDOXYLANASE; ENZYME FUNCTIONS; EXPRESSION SYSTEM; FUNCTIONAL CHARACTERIZATION; FUNCTIONAL TEST; GLYCOSYL HYDROLASES; HIGH LEVEL EXPRESSION; HYDROLYSIS PRODUCTS; INDUSTRIAL ENZYMES; LARGE SCALE PRODUCTIONS; P. PASTORIS; PASTORIS; PICHIA PASTORIS; PROTEIN SPECIES; PURIFIED ENZYME; SDS-PAGE; TRICHODERMA REESEI; XYLAN HYDROLYSIS; XYLANASE; XYLANASE ACTIVITY; XYLANASES;

DIFFUSERS (OPTICAL); HYDROLYSIS; INDUSTRIAL APPLICATIONS; METHANOL;

ENZYMES;

ALCOHOL OXIDASE; ALCOHOL OXIDASE 1; ENDO 1,4 BETA XYLANASE; GLYCOSIDASE; METHANOL; PLASMID VECTOR; RECOMBINANT ENZYME; UNCLASSIFIED DRUG; XYLAN; FUNGAL PROTEIN; RECOMBINANT PROTEIN; XYLAN ENDO 1,3 BETA XYLOSIDASE;

ARTICLE; BINDING ASSAY; CONTROLLED STUDY; CYTOLYSIS; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME KINETICS; ENZYME RELEASE; ENZYME SPECIFICITY; ENZYME SUBSTRATE; FERMENTATION; GENE EXPRESSION SYSTEM; HYDROLYSIS; INCUBATION TEMPERATURE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROMOTER REGION; PROTEIN STABILITY; THERMOSTABILITY; TRICHODERMA REESEI; ZYMOGRAPHY; BIOSYNTHESIS; ENZYMOLOGY; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; PICHIA; TEMPERATURE; TRICHODERMA;

HYPOCREA JECORINA; PICHIA PASTORIS;

CLONING, MOLECULAR; FUNGAL PROTEINS; GENE EXPRESSION; HYDROGEN-ION CONCENTRATION; KINETICS; PICHIA; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY; TEMPERATURE; TRICHODERMA; XYLAN ENDO-1,3-BETA-XYLOSIDASE;

EID: 67649631523     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-9-56     Document Type: Article

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