Acidophilic xylanase from Aureobasidium pullulans: Efficient expression and secretion in Pichia pastoris and mutational analysis

Journal of Bioscience and Bioengineering

Volumn 98, Issue 5, 2004, Pages 338-343

  Tanaka, Hidenori ^a   Okuno, Tomoko ^a   Moriyama, Satoshi ^a   Muguruma, Michio ^a   Ohta, Kazuyoshi ^a  

^a UNIVERSITY OF MIYAZAKI   (Japan)

Author keywords

Acidophilic enzyme; Aureobasidium pullulans; Heterologous expression; Pichia pastoris; Protein secretion; Signal sequence; Site directed mutagenesis; Xylanase

Indexed keywords

AMINO ACIDS; DNA; PROTEINS; YEAST;

CULTURE MEDIUM; MUTATIONAL ANALYSIS; PRECURSOR PROTEIN (XYNI); SIGNAL PEPTIDES;

MUTAGENS;

ACIDOPHILIC ENZYME; COMPLEMENTARY DNA; FUNGAL ENZYME; MUTANT PROTEIN; RECOMBINANT ENZYME; SIGNAL PEPTIDE; UNCLASSIFIED DRUG; XYLAN ENDO 1,3 BETA XYLOSIDASE;

AMINO TERMINAL SEQUENCE; ARTICLE; AUREOBASIDIUM PULLULANS; ENZYME ACTIVE SITE; ENZYME ASSAY; ENZYME PURIFICATION; GENE EXPRESSION; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PICHIA PASTORIS; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PROTEIN DETERMINATION; PROTEIN SECRETION; SITE DIRECTED MUTAGENESIS; TEMPERATURE; YEAST;

AUREOBASIDIUM; AUREOBASIDIUM PULLULANS; AUREOBASIDIUM PULLULANS VAR. MELANIGENUM; FUNGI; PICHIA; PICHIA PASTORIS; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 10944248996     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1389-1723(04)00292-0     Document Type: Article

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