Phosphorylatable short peptide conjugated low molecular weight chitosan for efficient siRNA delivery and target gene silencing

International Journal of Pharmaceutics

Volumn 422, Issue 1-2, 2012, Pages 445-453

  Kong, Fanqiang ^a   Liu, Guoping ^b   Sun, Bei ^a   Zhou, Shumin ^a   Zuo, Aijun ^a   Zhao, Ronglan ^a   Liang, Dongchun ^a  

^a TIANJIN MEDICAL UNIVERSITY   (China)

^b TIANJIN FIRST CENTRAL HOSPITAL   (China)

Author keywords

Chitosan; Low molecular weight; Phosphorylable peptide conjugation; siRNA; Unpacking; Uptake

Indexed keywords

CHITOSAN; FIREFLY LUCIFERASE; LIPOFECTAMINE; LOW MOLECULAR WEIGHT CHITOSAN; SMALL INTERFERING RNA; UNCLASSIFIED DRUG;

ARTICLE; BREAST CANCER; CANCER CELL CULTURE; CELL LYSATE; CELL MEMBRANE PERMEABILITY; DRUG DELIVERY SYSTEM; GENE SILENCING; GENE TARGETING; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; ISOTOPE LABELING; MOLECULAR WEIGHT; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; REPORTER GENE;

ACETYLATION; BREAST NEOPLASMS; CELL LINE, TUMOR; CHITOSAN; FEMALE; HUMANS; LIPIDS; LUCIFERASES; MOLECULAR WEIGHT; NANOPARTICLES; NUCLEIC ACID CONFORMATION; PARTICLE SIZE; PEPTIDES; PERMEABILITY; PHOSPHORYLATION; RNA INTERFERENCE; RNA, SMALL INTERFERING; TRANSFECTION;

EID: 84655167822     PISSN: 03785173     EISSN: 18733476     Source Type: Journal    
DOI: 10.1016/j.ijpharm.2011.10.041     Document Type: Article

References (21)

1. S.M. Elbashir, J. Harborth, W. Lendeckel, A. Yalcin, K. Weber, and T. Tuschl Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells Nature 411 2001 494 498 (Pubitemid 32494397)
2. F.D. Glen, K.W. Jonathan, A. Tommy, R. Francis, K. Anna, A. Veenu, P. Jerry, M.G. Alan, S. Nahum, and J.D. Masad Synergistic effects between analogs of DNA and RNA improve the potency of siRNA-mediated gene silencing Nucleic Acids Res. 38 2010 4547 4557
3. H.D. Han, L.S. Mangala, J.W. Lee, M.M. Shahzad, H.S. Kim, D. Shen, E.J. Nam, E.M. Mora, R.L. Stone, C. Lu, S.J. Lee, J.W. Roh, A.M. Nick, G. Lopez-Berestein, and A.K. Sood Targeted gene silencing using RGD-labeled chitosan nanoparticles Clin. Cancer Res. 16 2010 3910 3922
4. T. Ishii, Y. Okahata, and T. Sato Mechanism of cell transfection with plasmid/chitosan complexes Biochim. Biophys. Acta 1514 2001 51 64 (Pubitemid 32769928)
5. A.M. Ji, D. Su, O. Che, W.S. Li, L. Sun, Z.Y. Zhang, B. Yang, and F. Xu Functional gene silencing mediated by chitosan/siRNA nanocomplexes Nanotechnology 40 2009 405103
6. A.H. Kenneth, R.P. Soren, A.B. Mark, B. Flemming, D. Bent, and K. Jorgen Chitosan/siRNA nanoparticle-mediated TNF-α knockdown in peritoneal macrophages for anti-inflammatory treatment in a murine arthritis model Mol. Ther. 17 2008 162 168
7. D.W. Lee, K.S. Yun, H.S. Ban, W. Choe, S.K. Lee, and K.Y. Lee Preparation and characterization of chitosan/polyguluronate nanoparticles for siRNA delivery J. Control. Release 139 2009 146 152
8. M. Lee, J.W. Nah, Y. Kwon, J.J. Koh, K.S. Ko, and S.W. Kim Watersoluble and low molecular weight chitosan-based plasmid DNA delivery Pharm. Res. 18 2001 427 431 (Pubitemid 32592572)
9. D.C. Liang, W.G. Liu, A.J. Zuo, S.J. Sun, N. Cheng, G. Guo, J.Y. Zhang, and K. De Yao Pre-deliver chitosanase to cells: a novel strategy to improve gene expression by endocellular degradation-induced vector unpacking Int. J. Pharm. 314 2006 63 71
10. X. Liu, K.A. Howard, M. Dong, M.Ø. Andersen, U.L. Rahbek, M.G. Johnsen, O.C. Hansen, F. Besenbacher, and J. Kjems The influence of polymeric properties on chitosan/siRNA nanoparticle formulation and gene silencing Biomaterials 28 2008 1280 1288 (Pubitemid 44816373)
11. F.C. MacLaughlin, R.J. Mumper, J. Wang, J.M. Tagliaferri, I. Gill, and M. HinCHyiffe Chitosan and depolymerized chitosan oligomers as condensing carriers for in vivo plasmid delivery J. Control. Release 56 1998 259L 72
12. S.M. Noh, M.O. Park, G. Shim, S.E. Han, H.Y. Lee, J.H. Huh, M.S. Kim, J.J. Choi, K. Kim, I.C. Kwon, J.S. Kim, K.H. Baek, and Y.K. Oh Pegylated poly-l-arginine derivatives of chitosan for effective delivery of siRNA J. Control. Release 145 2010 159 164
13. M. Sandberg, T.A. Wallace, M.D. Godeny, D. VonDerLinden, and P.P. Sayeski Jak2 tyrosine kinase: a true jak of all trades? Cell Biochem. Biophys. 41 2004 207 232
14. P.A. Sharp RNA interference Genes Dev. 15 2001 485 490
15. B. Sun, R. Zhao, F. Kong, Y. Ren, A. Zuo, D. Liang, and J. Zhang Phosphorylatable short peptide conjugation for facilitating transfection efficacy of CS/DNA complex Int. J. Pharm. 2010 206 210
16. S. Techaarpornkul, S. Wongkupasert, P. Opanasopit, A. Apirakaramwong, J. Nunthanid, and U. Ruktanonchai Chitosan-Mediated siRNA delivery in vitro: effect of polymer molecular weight, concentration and salt forms AAPS Pharm. Sci. Technol. 11 2010 64 72
17. T.A. Wallace, D. VonDerLinden, K. He, S.J. Frank, and P. PSayeski Microarray analyses identify JAK2 tyrosine kinase as a key mediator of ligand-independent gene expression Am. J. Physiol. Cell. Physiol. 287 2004 981 991
18. W. Weecharangsan, P. Opanasopit, T. Ngawhirunpat, T. Rojanarata, and A. Apirakaramwong Chitosan lactate as a nonviral gene delivery vector in COS-1 cells AAPS Pharm. Sci. Technol. 7 2006 E1 E6
19. Y. Wu, W. Wang, Y. Chen, K. Huang, X. Shuai, Q. Chen, X. Li, and G. Lian The investigation of polymer-siRNA nanoparticle for gene therapy of gastric cancer in vitro Int. J. Nanomed. 5 2010 129 136
20. X. Zhao, S.B. Yu, F.L. Wu, Z.B. Mao, and C.L. Yu Transfection of primary chondrocytes using chitosan-pEEGFP nanoparticles J. Control. Release 112 2006 223 228
21. A. Zuo, B. Sun, D. Liang, W. Liu, R. Zhao, G. Guo, N. Chengn, J. Zhang, and K. Yao Improved transfection efficiency of CS/DNA complex by co-transfected chitosanase gene Int. J. Pharm. 352 2008 302 308