Improved transfection efficiency of CS/DNA complex by co-transfected chitosanase gene

International Journal of Pharmaceutics

Volumn 352, Issue 1-2, 2008, Pages 302-308

  Zuo, Aijun ^a,b   Sun, Pei ^a,b   Liang, Dongchun ^a,b   Liu, Wenguang ^c   Zhao, Ronglan ^a,b   Guo, Gang ^a,b   Cheng, Nan ^c   Zhang, Jingyu ^a,b   Yao, Kangde ^c  

^a TIANJIN MEDICAL UNIVERSITY   (China)

^b Key Lab of Hormone and development   (China)

^c TIANJIN UNIVERSITY   (China)

Author keywords

Chitosan; Chitosanase gene; Co transfection

Indexed keywords

BETA GALACTOSIDASE; CHITOSANASE; DNA; PLASMID VECTOR;

ANIMAL CELL; ARTICLE; ASPERGILLUS FUMIGATUS; BACTERIAL CELL; COMPLEX FORMATION; CONTROLLED STUDY; DEGRADATION; ENZYME ACTIVITY; FLUORESCENCE MICROSCOPY; GENE EXPRESSION; GENE MUTATION; GENE TARGETING; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; MOLECULAR CLONING; MOUSE; MYOBLAST; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN PURIFICATION; QUANTITATIVE ANALYSIS; REPORTER GENE;

ACTIVE TRANSPORT, CELL NUCLEUS; ANIMALS; ASPERGILLUS FUMIGATUS; CELL LINE; CELL LINE, TUMOR; CELL NUCLEUS; CHITOSAN; CLONING, MOLECULAR; DNA; FUNGAL PROTEINS; GENES, REPORTER; GENETIC VECTORS; GLYCOSIDE HYDROLASES; HUMANS; MICE; MICROSCOPY, FLUORESCENCE; MUTAGENESIS, SITE-DIRECTED; RECOMBINANT PROTEINS; TRANSFECTION;

EID: 39549096259     PISSN: 03785173     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ijpharm.2007.10.042     Document Type: Article

References (22)

1. Aral C., and Akbuga J. Preparation and in vitro transfection efficiency of chitosan microspheres containing plasmid DNA:poly(l-lysine) complexes. J. Pharm. Pharm. Sci. 6 (2003) 321-326
2. Cheng C.Y., Chang C.H., Wu Y.J., and Li Y.K. Exploration of glycosyl hydrolase family 75, a chitosanase from Aspergillus fumigatus. J. Biol. Chem. 281 (2006) 3137-3144
3. Chen J., Yang W.L., Li G., Qian J., Xue J.L., Fu S.K., and Lu D.R. Transfection of mEpo gene to intestinal epithelium in vivo mediated by oral delivery of chitosan-DNA nanoparticles. World J. Gastroenterol. 10 (2004) 112-116
4. Corsi K., Chellat F., Yahia H., and Fernandes J.C. Mesenchymal stem cells, MG63 and HEK293 transfection using chitosan-DNA nanoparticles. Biomaterials 24 (2003) 1255-1264
5. Iqbal M., Lin W., Jabbal-Gill I., Davis S.S., Steward M.W., and Illum L. Nasal delivery of chitosan-DNA plasmid expressing epitopes of respiratory syncytial virus (RSV) induces protective CTL responses in BALB/c mice. Vaccine 21 (2003) 1478-1485
6. Kim T.H., Park I.Y., Nah J.W., Choi Y.J., and Cho C.S. Galactosylated chitosan/DNA nanopaticles prepared using water-soluble chitosan as a gene carrier. Biomaterials 25 (2004) 3783-3792
7. Kim Y.H., Gihm S.H., and Park C.R. Structural characteristics of size-controlled self-aggregates of deoxycholic acid-modified chitosan and their application as a DNA delivery carrier. Bioconjug. Chem. 12 (2001) 932-938
8. Kim T.H., Ihm J.E., Choi Y.J., Nah J.W., and Cho C.S. Efficient gene delivery by urocanic acid-modified chitosan. J. Controlled Release 93 (2003) 389-402
9. Liang D.C., Liu W.G., Zuo A.J., Sun S.J., Cheng N., Guo G., Zhang J.Y., and Yao K.De. Pre-deliver chitosanase to cells: A novel strategy to improve gene expression by endocellular degradation-induced vector unpacking. Int. J. Pharm. 314 (2006) 63-71
10. Liu W.G., Zhang X., Sun S.J., Sun G.J., Yao K.D., Liang D.C., Guo G., and Zhang J.Y. N-Alkylated chitosan as a potential nonviral vector for gene transfection. Bioconjug. Chem. 14 (2003) 782-789
11. Mansouri S., Lavigne P., Corsi K., Benderdour M., Beaumont E., and Fernandes J.C. Chitosan-DNA nanoparticles as non-viral vectors in gene therapy: strategies to improve transfection efficacy. Eur. J. Pharm. Biopharm. 57 (2004) 1-8
12. Mansouri S., Cuie Y., Winnik F., Shi Q., Lavigne P., Benderdour M., Beaumont E., and Fernandes J.C. Characterization of folate-chitosan-DNA nanoparticles for gene therapy. Biomaterials 27 (2006) 2060-2065
13. Ozgel G., and Akbuga J. In vitro characterization and transfection of IL-2 gene complexes. Int. J. Pharm. 315 (2006) 44-51
14. Park I.K., Ihm J.E., Park Y.H., Choi Y.J., Kim S.I., Kim W.J., Akaike T., and Cho C.S. Galactosylated chitosan (GC)-graft-poly(vinyl pyrrolidone) (PVP) as hepatocyte-targeting DNA carrier, preparation and physicochemical characterization of GC-graft-PVP/DNA complex (1). J. Controlled Release 86 (2004) 349-359
15. Prabaharan M., and Mano J.F. Chitosan-based particles as controlled drug delivery systems. Drug Deliv. 12 (2005) 41-57
16. Romoren K., Pedersen S., Smistad G., Evensen O., and Thu B.J. The influence of formulation variables on in vitro transfection efficiency and physicochemical properties of chitosan-based polyplexes. Int. J. Pharm. 261 (2003) 115-127
17. Sato T., Ishii T., and Okahata Y. In vitro gene delivery mediated by chitosan. Effect of pH, serum, and molecular mass of chitosan on the transfection efficiency. Biomaterials 22 (2001) 2075-2080
18. Wong K., Sun G., Zhang X., Dai H., Liu Y., He C., and Leong K.W. PEI-g-chitosan, a novel gene delivery system with transfection efficiency comparable to polyethylenimine in vitro and after liver administration in vivo. Bioconjug. Chem. 17 (2006) 152-158
19. Yoo H.S., Lee J.E., Chung H., Kwon I.C., and Jeong S.Y. Self-assembled nanoparticles containing hydrophobically modified glycol chitosan for gene delivery. J. Controlled Release 103 (2005) 235-243
20. Yun Y.H., Jiang H., Chan R., and Chen W. Sustained release of PEG-g-chitosan complexed DNA from poly(lactide-co-glycolide). J. Biomater. Sci. Polym. Ed. 16 (2005) 1359-1378
21. Zhang X.Y., Dai A.L., Kuroiwa K., Kodaira R., Nogawa M., Shimosaka M., and Okazaki M. Cloning and characterization of a chitosanase gene from the koji mold Aspergillus oryzae strain IAM 2660. Biosci. Biotechnol. Biochem. 65 (2001) 977-981
22. Zhang X., Yu C., Xu S., Zhang C., Tang T., and Dai K. Direct chitosan-mediated gene delivery to the rabbit knee joints in vitro and in vivo. Biochem. Biophys. Res. Commun. 341 (2006) 202-208