Polyphenolic fraction of Pilea microphylla (L.) protects Chinese hamster lung fibroblasts against γ-radiation-induced cytotoxicity and genotoxicity

Environmental Toxicology and Pharmacology

Volumn 33, Issue 1, 2012, Pages 107-119

  Paul, Piya ^a   Bansal, Punit ^a   Nayak, Pawan G ^a   Pannakal, Steve Thomas ^a   Priyadarsini, K I ^a   Unnikrishnan, M K ^b  

^a MANIPAL COLLEGE OF PHARMACEUTICAL SCIENCES   (India)

^b BHABHA ATOMIC RESEARCH CENTRE   (India)

Author keywords

Radiation; Cytotoxicity; Genotoxicity; Pilea microphylla; Polyphenols

Indexed keywords

CHLOROGENIC ACID; PILEA MICROPHYLLA EXTRACT; PLANT EXTRACT; QUERCETIN; RUTOSIDE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL PROTECTION; CHINESE HAMSTER; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOTOXICITY; DNA DAMAGE; FENTON REACTION; GAMMA RADIATION; GENOTOXICITY; LIPID PEROXIDATION; LUNG FIBROBLAST; MEDICINAL PLANT; NONHUMAN; PILEA MICROPHYLLA; PRIORITY JOURNAL; RADIATION DOSE; RADIATION PROTECTION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

ANIMALS; CELL LINE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; COMET ASSAY; CRICETINAE; CRICETULUS; DNA DAMAGE; FIBROBLASTS; FREE RADICAL SCAVENGERS; GAMMA RAYS; HUMANS; LIPID PEROXIDATION; LUNG; MICRONUCLEUS TESTS; PLANT EXTRACTS; POLYPHENOLS; RADIATION-PROTECTIVE AGENTS; URTICACEAE;

CRICETULUS GRISEUS; PILEA MICROPHYLLA;

EID: 84455202520     PISSN: 13826689     EISSN: 18727077     Source Type: Journal    
DOI: 10.1016/j.etap.2011.09.007     Document Type: Article

References (49)

1. Andreassen C.N., Grau C., Lindegaard J.C. Chemical radioprotection: a critical review of amifostine as a cytoprotector in radiotherapy. Semin. Radiat. Oncol. 2003, 13:62-72.
2. Aoki M., Furusawa Y., Yamada T. LET dependency of heavy-ion induced apoptosis in V79 cells. J. Radiat. Res. 2000, 41:163-175.
3. Archana P., Nageshwar Rao B., Ballal M., Satish Rao B. Thymol, a naturally occurring monocyclic dietary phenolic compound protects Chinese hamster lung fibroblasts from radiation-induced cytotoxicity. Mutat. Res. 2009, 680:70-77.
4. Arora R., Gupta D., Chawla R., Sagar R., Sharma A., Kumar R. Radioprotection by plant products: present status and future prospects. Phytother. Res. 2005, 19:1-22.
5. Banerjee A., Kunwar A., Mishra B., Priyadarsini K.I. Concentration dependent antioxidant/pro-oxidant activity of curcumin studies from AAPH induced hemolysis of RBCs. Chem. Biol. Interact. 2008, 174:134-139.
6. Bansal P., Paul P., Mudgal J., Nayak P.G., Thomas Pannakal S., Priyadarsini K.I., Unnikrishnan M.K. Antidiabetic, antihyperlipidemic and antioxidant effects of the flavonoid rich fraction of Pilea microphylla (L.) in high fat diet/streptozotocin-induced diabetes in mice. Exp. Toxicol. Pathol. 2011, 10.1016/j.etp.2010.12.009.
7. Benkovic V., Knezevic A.H., Dikic D., Lisicic D., Orsolic N., Basic I., Kosalec I., Kopjar N. Radioprotective effects of propolis and quercetin in gamma-irradiated mice evaluated by the alkaline comet assay. Phytomedicine 2008, 15:851-858.
8. Carmichael J., DeGraff W.G., Gazdar A.F., Minna J.D., Mitchell J.B. Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res. 1987, 47:936-941.
9. Chaudhary P., Shukla S.K., Kumar I.P., Namita I., Afrin F., Sharma R.K. Radioprotective properties of apple polyphenols: an in vitro study. Mol. Cell. Biochem. 2006, 288:37-46.
10. Devi P.U., Bisht K.S., Vinitha M. A comparative study of radioprotection by Ocimum flavonoids and synthetic aminothiol protectors in the mouse. Br. J. Radiol. 1998, 71:782-784.
11. Devi P.U., Ganasoundari A., Vrinda B., Srinivasan K.K., Unnikrishnan M.K. Radiation protection by the ocimum flavonoids orientin and vicenin: mechanisms of action. Radiat. Res. 2000, 154:455-460.
12. Devipriya N., Sudheer A.R., Srinivasan M., Menon V.P. Quercetin ameliorates gamma radiation-induced DNA damage and biochemical changes in human peripheral blood lymphocytes. Mutat. Res. 2008, 654:1-7.
13. Fenech M., Chang W.P., Kirsch-Volders M., Holland N., Bonassi S., Zeiger E. Human micronucleus (HMN) project: detailed description of the scoring criteria for the cytokinesis-block micronucleus assay using isolated lymphocytes. Mutat. Res. 2003, 534:65-75.
14. Ferreira J.F., Luthria D.L., Sasaki T., Heyerick A. Flavonoids from Artemisia annua L. as antioxidants and their potential synergism with artemisinin against malaria and cancer. Molecules 2010, 15:3135-3170.
15. Goodman G.E., Thornquist M.D., Balmes J., Cullen M.R., Meyskens F.L., Omenn G.S., Valanis B., Williams J.H. The beta-carotene and retinol efficacy trial: incidence of lung cancer and cardiovascular disease mortality during 6-year follow-up after stopping beta-carotene and retinol supplements. J. Natl. Cancer Inst. 2004, 96:1743-1750.
16. Gupta V.K. Comprehensive bioactive natural products. Efficacy, Safety & Clinical Evaluation (Part 1) 2010, 1-29. Studium Press LLC, U.S.A. R. Khanna, S.P. Agarwal, R.K. Khar (Eds.).
17. Hosseinimehr S.J. Foundation review: trends in the development of radioprotective agents. Drug Discov. Today 2007, 12:794-805.
18. Hosseinimehr S.J., Tavakoli H., Pourheidari G., Sobhani A., Shafiee A. Radioprotective effects of citrus extract against gamma-irradiation in mouse bone marrow cells. J. Radiat. Res. 2003, 44:237-241.
19. Hosseinimehr S.J., Zakaryaee V., Ahmadi A., Akhlaghpoor S. Radioprotective effects of chlorogenic acid against mortality induced by gamma-irradiation in mice. Methods Find. Exp. Clin. Pharmacol. 2008, 30:13-16.
20. Inouye S. Site-specific cleavage of double-strand DNA by hydroperoxide of linoleic acid. FEBS Lett. 1984, 172:231-234.
21. Kelly G.S. The interaction of cigarette smoking and antioxidants. Part I: diet and carotenoids. Altern. Med. Rev. 2002, 7:370-388.
22. Kleinerman R.A. Cancer risks following diagnostic and therapeutic radiation exposure in children. Pediatr. Radiol. 2006, 36:121-125.
23. Kumar M., Samarth R., Kumar M., Selvan S.R., Saharan B., Kumar A. Protective effect of Adhatoda vascia Nees against radiation-induced damage at cellular, biochemical and chromosomal levels in swiss albino mice. Evid. Based Complement. Altern. Med. 2007, 4:343-350.
24. Kunwar A., Bansal P., Kumar S.J., Bag P.P., Paul P., Reddy N.D., Kumbhare L.B., Jain V.K., Chaubey R.C., Unnikrishnan M.K., Priyadarsini K.I. In vivo radioprotection studies of 3,3'-diselenodipropionic acid, a selenocystine derivative. Free Radic. Biol. Med. 2010, 48:399-410.
25. Kyoung A.K., Rui Z., Kyoung H.L., Sungwook C., Bum J.K., Young S.K., Jae W.P., Nam H.L., Jin W.H. Protective effect of triphlorethol-A from Ecklonia cava against ionizing radiation in vitro. J. Radiat. Res. 2006, 47:61-68.
26. Lemmens R.H.M.J., Bunyapraphatsara N. Plant Resources of South-East Asia: Medicinal and Poisonous Plants 3 2003, Backhuys Publishers, Leiden.
27. Manach C., Williamson G., Morand C., Scalbert A., Rémésy C. Bioavailability and bioefficacy of polyphenols in humans. I. Review of 97 bioavailability studies. Am. J. Clin. Nutr. 2005, 81:S230-S242.
28. Mantena S.K., Unnikrishnan M.K., Joshi R., Radha V., Devi P.U., Mukherjee T. In vivo radioprotection by 5-aminosalicylic acid. Mutat. Res. 2008, 650:63-79.
29. Maurya D.K., Devasagayam T.P., Nair C.K. Some novel approaches for radioprotection and the beneficial effect of natural products. Indian J. Exp. Biol. 2006, 44:93-114.
30. Mishra B., Priyadarsini K.I., Kumar M.S., Unnikrishnan M.K., Mohan H. Effect of O-glycosilation on the antioxidant activity and free radical reactions of a plant flavonoid, chrysoeriol. J. Bioorg. Med. Chem. 2003, 11:2677-2685.
31. Newman D.J., Cragg G.M. Natural products as sources of new drugs over the last 25 years. J. Nat. Prod. 2007, 70:461-477.
32. Nijveldt R.J., van Nood E., van Hoorn D.E., Boelens P.G., van Norren K., van Leeuwen P.A. Flavonoids: a review of probable mechanisms of action and potential applications. Am. J. Clin. Nutr. 2001, 74:418-425.
33. Ohkawa H., Ohishi N., Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 1979, 95:351-358.
34. Omenn G.S., Goodman G.E., Thornquist M.D., Balmes J., Cullen M.R., Glass A., Keogh J.P., Meyskens F.L., Valanis B., Williams J.H., Barnhart S., Cherniack M.G., Brodkin C.A., Hammar S. Risk factors for lung cancer and for intervention effects in CARET, the beta-carotene and retinol efficacy trial. J. Natl. Cancer Inst. 1996, 88:1550-1559.
35. Omenn G.S., Goodman G.E., Thornquist M.D., Balmes J., Cullen M.R., Glass A., Keogh J.P., Meyskens F.L., Valanis B., Williams J.H., Barnhart S., Hammar S. Effects of a combination of beta carotene and vitamin A on lung cancer and cardiovascular disease. N. Engl. J. Med. 1996, 334:1150-1155.
36. Parihar V.K., Prabhakar K.R., Veerapur V.P., Priyadarsini K.I., Unnikrishnan M.K., Rao C.M. Anticlastogenic activity of morin against whole body gamma irradiation in Swiss albino mice. Eur. J. Pharmacol. 2007, 557:58-65.
37. Prabhakar K.R., Veerapur V.P., Bansal P., Parihar V.K., Reddy Kandadi M., Bhagath Kumar P., Priyadarsini K.I., Unnikrishnan M.K. Antioxidant and radioprotective effect of the active fraction of Pilea microphylla (L.) ethanolic extract. Chem. Biol. Interact. 2007, 165:22-32.
38. Prabhakar K.R., Veeresh V.P., Vipan K., Sudheer M., Priyadarsini K.I., Satish R.B., Unnikrishnan M.K. Bioactivity-guided fractionation of Coronopus didymus: a free radical scavenging perspective. Phytomedicine 2006, 13:591-595.
39. Rajagopalan R., Kagiya T.V., Nair C.K. Radiosensitizer sanazole (AK-2123) enhances gamma-radiation-induced apoptosis in murine fibrosarcoma. J. Radiat. Res. 2003, 44:359-365.
40. Rasoanaivo P., Wright C.W., Willcox M.L., Gilbert B. Whole plant extracts versus single compounds for the treatment of malaria: synergy and positive interactions. Malar. J. 2011, 10.
41. Renvoizé C., Biola A., Pallardy M., Bréard J. Apoptosis: identification of dying cells. Cell Biol. Toxicol. 1998, 14:111-120.
42. Riley P.A. Free radicals in biology: oxidative stress and the effects of ionizing radiation. Int. J. Radiat. Biol. 1994, 65:27-33.
43. Singh N.P. Microgels for estimation of DNA strand breaks, DNA protein crosslinks and apoptosis. Mutat. Res. 2000, 455:111-127.
44. Singh U., Kunwar A., Srinivasan R., Nanjan M.J., Priyadarsini K.I. Differential free radical scavenging activity and radioprotection of Caesalpinia digyna extracts and its active constituent. J. Radiat. Res. 2009, 50:425-433.
45. Srinivasan M., Sudheer A.R., Rajasekaran K.N., Menon V.P. Effect of curcumin analog on γ-radiation-induced cellular changes in primary culture of isolated rat hepatocytes in vitro. Chem. Biol. Interact. 2008, 176:1-8.
46. Veerapur V.P., Prabhakar K.R., Parihar V.K., Bansal P., Srinivasan K.K., Priyadarsini K.I., Unnikrishnan M.K. Antidiabetic, hypolipidaemic and antioxidant activity of Dodonaea viscosa aerial parts in streptozotocin-induced diabetic rats. Int. J. Phytomedicine 2010, 2:59-70.
47. Veerapur V.P., Prabhakar K.R., Parihar V.K., Kandadi M.R., Ramakrishana S., Mishra B., Satish Rao B.S., Srinivasan K.K., Priyadarsini K.I., Unnikrishnan M.K. Ficus racemosa stem bark extract: a potent antioxidant and a probable natural radioprotector. Evid. Based Complement. Altern. Med. 2009, 6:317-324.
48. WHO, 1977. Use of ionizing radiation and radionuclides on human beings for medical research, training, and nonmedical purposes. Report of a WHO Expert Committee. World Health Organ. Tech. Rep. Ser. 611, pp. 1-39.
49. Zhou F.X., Liao Z.K., Dai J., Xiong J., Xie C.H., Luo Z.G., Liu S.Q., Zhou Y.F. Radiosensitization effect of zidovudine on human malignant glioma cells. Biochem. Biophys. Res. Commun. 2007, 354:351-356.