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Volumn 16, Issue 23, 2004, Pages 4467-4476

Homogeneous fluorescence-based DNA detection with water-soluble conjugated polymers

Author keywords

[No Author keywords available]

Indexed keywords

BIOASSAY; COLORIMETRY; DNA; ELECTROSTATICS; FLUORESCENCE; ORGANIC POLYMERS; POLYELECTROLYTES; SELF ASSEMBLY; SOLUBILITY;

EID: 8444248899     PISSN: 08974756     EISSN: None     Source Type: Journal    
DOI: 10.1021/cm049587x     Document Type: Review
Times cited : (603)

References (74)
  • 46
    • 0036813339 scopus 로고    scopus 로고
    • Although anionic poly(phenyleneethynylene), such as biotinylated PPEs (1 in Figure 1), have also been used for DNA analysis, strepavidine-derivated polystyrene microspheres are required to bind both polymers and biotinylated probes. See references: (a) Kushon, S. A.; Ley, K. D.; Bradford, K.; Jones, R. M.; McBranch, D.; Whitten, D. Langmuir 2002, 18, 7245. (b) Kushon, S. A.; Bradford, K.; Marin, V.; Suhrada, C.; Armitage, B. A.; McBranch, D.; Whitten, D. Langmuir 2003, 19, 6456.
    • (2002) Langmuir , vol.18 , pp. 7245
    • Kushon, S.A.1    Ley, K.D.2    Bradford, K.3    Jones, R.M.4    McBranch, D.5    Whitten, D.6
  • 47
    • 0042069981 scopus 로고    scopus 로고
    • Although anionic poly(phenyleneethynylene), such as biotinylated PPEs (1 in Figure 1), have also been used for DNA analysis, strepavidine-derivated polystyrene microspheres are required to bind both polymers and biotinylated probes. See references: (a) Kushon, S. A.; Ley, K. D.; Bradford, K.; Jones, R. M.; McBranch, D.; Whitten, D. Langmuir 2002, 18, 7245. (b) Kushon, S. A.; Bradford, K.; Marin, V.; Suhrada, C.; Armitage, B. A.; McBranch, D.; Whitten, D. Langmuir 2003, 19, 6456.
    • (2003) Langmuir , vol.19 , pp. 6456
    • Kushon, S.A.1    Bradford, K.2    Marin, V.3    Suhrada, C.4    Armitage, B.A.5    McBranch, D.6    Whitten, D.7
  • 56
    • 8444247335 scopus 로고    scopus 로고
    • note
    • This result indicates that the rigid polyfluorene structure most likely does not adapt to the DNA conformation upon complexation. They also suggest that binding into the grooves of DNA, like other cationic dyes such as ethidium bromide, is not a dominant form of association. Any twisting to accommodate such groove binding or helical conformation would likely shorten the effective conjugation length in the conducting polymer and thus blue-shift the emission. This information strengthens the idea that interactions between DNA and 4 are largely due to electrostatics.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.