Homogeneous single-label biochemical ras activation assay using time-resolved luminescence

Analytical Chemistry

Volumn 83, Issue 24, 2011, Pages 9230-9233

  Martikkala, Eija ^a   Veltel, Stefan ^b   Kirjavainen, Jonna ^a   Rozwandowicz Jansen, Anita ^a   Lamminmäki, Urpo ^a   Hänninen, Pekka ^a   Härmä, Harri ^a  

^a UNIVERSITY OF TURKU   (Finland)

^b VTT TECHNICAL RESEARCH CENTRE OF FINLAND   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVITY ASSAYS; ASSAY OPTIMIZATION; CELL SIGNALING; COEFFICIENT OF VARIATION; GTP-BINDING PROTEINS; GTPASES; GUANINE NUCLEOTIDE EXCHANGE FACTORS; HIGH-THROUGHPUT SCREENING; IMMUNOBLOTTING; IMPROVED METHODS; LOW CONCENTRATIONS; POTENTIAL DRUG; PRIMARY SCREENING; PURIFIED PROTEIN; RADIOACTIVE LABELS; RAPID DETERMINATION; RESONANCE ENERGY TRANSFER; SIGNAL-TO-BACKGROUND RATIO; SIGNALING PATHWAYS; TIME-RESOLVED LUMINESCENCE;

BINDING ENERGY; ENERGY TRANSFER; ONCOGENIC VIRUSES; PROTEINS; PURIFICATION; QUENCHING; SIGNAL DETECTION; TERBIUM;

ASSAYS;

GUANINE; GUANOSINE TRIPHOSPHATE; RAS PROTEIN; TERBIUM;

ARTICLE; CHEMISTRY; ENERGY TRANSFER; HIGH THROUGHPUT SCREENING; LUMINESCENCE; METABOLISM;

ENERGY TRANSFER; GUANINE; GUANOSINE TRIPHOSPHATE; HIGH-THROUGHPUT SCREENING ASSAYS; LUMINESCENT MEASUREMENTS; RAS PROTEINS; TERBIUM;

EID: 83655192576     PISSN: 00032700     EISSN: None     Source Type: Journal    
DOI: 10.1021/ac202723h     Document Type: Article

References (30)

1. Oxford, G.; Theodorescu, D Cancer Lett. 2003, 189, 117-128
2. Bos, J. Cancer Res. 1989, 49, 4682-4689
3. Takai, Y; Sasaki, T.; Matozaki, T. Physiol. Rev. 2001, 81, 153-208
4. Vetter, I. R.; Wittinghofer, A. Science 2001, 294, 1299-304
5. Gibbs, J.; Schaber, M.; Marshall, M.; Scolnick, E.; Sigal, I J. Biol. Chem. 1987, 262, 10426-10429
6. Satoh, T.; Endo, M.; Nakamura, S.; Kaziro, Y FEBS Lett. 1988, 236, 185-189
7. Taylor, S.; Shalloway, D. Curr. Biol. 1996, 6, 1621-7
8. Spinosa, M.; Progida, C.; De Luca, A.; Rosaria Colucci, A.; Alifano, P.; Bucci, C. J. Neurosci. 2008, 28, 1640-1648
9. Gasmi-Seabrook, G.; Marshall, C.; Cheung, M.; Kim, B.; Wang, F.; Jang, Y.; Mak, T.; Stambolic, V.; Ikura, M J. Biol. Chem. 2010, 285, 5137-5145
10. Korlach, J.; Baird, D.; Heikal, A.; Gee, K.; Hoffman, G.; Webb, W. Proc. Natl. Acad. Sci. U.S.A. 2004, 101, 2800-2805
11. Rojas, R.; Kimple, R.; Rossman, K.; Siderovski, D.; Sondek, J. Comb. Chem. High Throughput Screen. 2003, 6, 409-418
12. Hadjuk, P.; Burns, D. Comb. Chem. High Throughput Screen. 2002, 5, 613-621
13. Härmä, H.; Rozwandowicz-Jansen, A.; Martikkala, E.; Frang, H.; Hemmilä, I.; Sahlberg, N.; Fey, V.; Perälä, M.; Hänninen, P. J. Biomol. Screen. 2009, 14, 936-943
14. Härmä, H.; Sarrail, G.; Kirjavainen, J.; Martikkala, E.; Hemmilä, I.; Hänninen, P. Anal. Chem. 2010, 82, 892-897
15. Rozwandowicz-Jansen, A.; Laurila, J.; Martikkala, E.; Frang, H.; Hemmilä, I.; Scheinin, M.; Hänninen, P.; Härmä, H. J. Biomol. Screen. 2010, 15, 261-267
16. Martikkala, E.; Rozwandowicz-Jansen, A.; Hänninen, P.; Petäjä-Repo, U.; Härmä, H. J. Biomol. Screen. 2011, 16, 356-362
17. Huttunen, R.; Shweta; Martikkala, E.; Lahdenranta, M.; Virta, P.; Hänninen, P.; Härmä, H. Anal. Biochem. 2011, 415, 27-31
18. Vuojola, J.; Lamminmäki, U.; Soukka, T. Anal. Chem. 2009, 81, 5033-5038
19. Lenzen, C.; Cool, R.; Prinz, H.; Kuhlmann, J.; Wittinhofer, A. Biochemistry 1998, 37, 7420-7430
20. Brownbridge, G.; Lowee, P.; Moore, K.; Skinner, R.; Webb, M. J. Biol. Chem. 1993, 268, 10914-10919
21. Soini, E.; Hemmila, I Clin. Chem. 1979, 25, 353-361
22. Jameson, E.; Cunliffe, J.; Neubig, R.; Sunahara, R.; Kennedy, R Anal. Chem. 2003, 75, 4297-4304
23. Ford, B.; Boykevisch, B.; Zhao, C.; Kunzelmann, S.; Bar-Sagi, D.; Herrmann, C.; Nassar, N. Biochemistry 2009, 48, 11449-11457
24. Eberth, A.; Dvorsky, R.; Becker, C.; Beste, A.; Goody, R.; Ahmadian, M. Biol. Chem. 2005, 386, 1105-1114
25. Gureasko, J; Galush, J; Boykevisch, S.; Sondermann, H; Bar-Sagi, D; Groves, J; Kuriyan, J. Nat. Struct. Mol. Biol. 2008, 15, 452-461
26. Mazhab-Jafari, M.; Marshall, C.; Smith, M.; Gasmi-Seabrook, G.; Stambolic, V.; Rottapel, R.; Neel, B.; Ikura, M. J. Biol. Chem. 2010, 285, 5132-5136
27. Zhang, J.; Matthews, C. Biochemistry 1998a, 37, 14881-14890
28. Zhang, J.; Matthews, C. Biochemistry 1998b, 37, 14891-14899
29. Itzen, A.; Pylypenko, O.; Goody, R.; Alexandrov, K.; Rak, A. EMBO J. 2006, 25, 1445-1455
30. Zhang, B.; Zhang, Y.; Shacter, E.; Zheng, Y. Biochemistry 2005, 44, 2566-2576