A homogeneous single-label time-resolved fluorescence cAMP assay

Journal of Biomolecular Screening

Volumn 16, Issue 3, 2011, Pages 356-362

  Martikkala, Eija ^a   Rozwandowicz Jansen, Anita ^a   Hänninen, Pekka ^a   Petäjä Repo, Ulla ^b   Härmä, Harri ^a  

^a UNIVERSITY OF TURKU   (Finland)

^b UNIVERSITY OF OULU   (Finland)

Author keywords

cAMP; cell signaling; cell based assay; functional assay; G protein coupled receptor; LANthanide Chelation Excitation (LANCE); QRET assay; time resolved fluorescence; TR FRET

Indexed keywords

ADENYLATE CYCLASE; BETA 2 ADRENERGIC RECEPTOR; CYCLIC AMP; DELTA OPIATE RECEPTOR; EUROPIUM; FORSKOLIN; INHIBITORY GUANINE NUCLEOTIDE BINDING PROTEIN; STIMULATORY GUANINE NUCLEOTIDE BINDING PROTEIN;

ANTIGEN BINDING; ARTICLE; CELL STRAIN HEK293; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; ENZYME ASSAY; FLUORESCENCE RESONANCE ENERGY TRANSFER; GENE OVEREXPRESSION; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; LIMIT OF DETECTION; PRIORITY JOURNAL; QUENCHING RESONANCE ENERGY TRANSFER; REPRODUCIBILITY; TIME RESOLVED FLUORESCENCE;

ADRENERGIC BETA-2 RECEPTOR AGONISTS; BIOCHEMISTRY; CELL COUNT; CYCLIC AMP; FLUORESCENCE RESONANCE ENERGY TRANSFER; GENE EXPRESSION REGULATION; HEK293 CELLS; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; RECEPTORS, G-PROTEIN-COUPLED; RESEARCH DESIGN;

EID: 79954586393     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057110397356     Document Type: Article

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