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Volumn 77, Issue 21, 2011, Pages 7595-7604

Generalized schemes for high-throughput manipulation of the Desulfovibrio vulgaris genome

Author keywords

[No Author keywords available]

Indexed keywords

AFFINITY PURIFICATION; BIOLOGICAL APPLICATIONS; DESULFOVIBRIO VULGARIS; DIVERSE SPECIES; DOWNSTREAM APPLICATIONS; FUNCTIONAL GENOMICS; GENE FUSION; GENE REPLACEMENT; HIGH THROUGHPUT IMPLEMENTATION; HIGH-THROUGHPUT; METABOLIC PATHWAYS; PROTEIN LOCALIZATION; PROTEIN-PROTEIN INTERACTIONS; SUICIDE VECTORS;

EID: 83155185432     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.05495-11     Document Type: Article
Times cited : (10)

References (51)
  • 1
    • 0031970775 scopus 로고    scopus 로고
    • Effects of the Escherichia coli DNA-binding protein H-NS on rRNA synthesis in vivo
    • Afflerbach, H., O. Schroder, and R. Wagner. 1998. Effects of the Escherichia coli DNA-binding protein H-NS on rRNA synthesis in vivo. Mol. Microbiol. 28:641-653.
    • (1998) Mol. Microbiol. , vol.28 , pp. 641-653
    • Afflerbach, H.1    Schroder, O.2    Wagner, R.3
  • 2
    • 0030928287 scopus 로고    scopus 로고
    • Repressor induced site-specific binding of HU for transcriptional regulation
    • Aki, T., and S. Adhya. 1997. Repressor induced site-specific binding of HU for transcriptional regulation. EMBO J. 16:3666-3674.
    • (1997) EMBO J , vol.16 , pp. 3666-3674
    • Aki, T.1    Adhya, S.2
  • 3
    • 22244489084 scopus 로고    scopus 로고
    • The MicrobesOnline Web site for comparative genomics
    • Alm, E. J., et al. 2005. The MicrobesOnline Web site for comparative genomics. Genome Res. 15:1015-1022.
    • (2005) Genome Res , vol.15 , pp. 1015-1022
    • Alm, E.J.1
  • 5
    • 31544450286 scopus 로고    scopus 로고
    • Construction of Escherichia coli K-12 in-frame, singlegene knockout mutants: the Keio collection
    • Baba, T., et al. 2006. Construction of Escherichia coli K-12 in-frame, singlegene knockout mutants: the Keio collection. Mol. Syst. Biol. 2:2006.0008.
    • (2006) Mol. Syst. Biol. , vol.2
    • Baba, T.1
  • 6
    • 42049092059 scopus 로고    scopus 로고
    • Markerless multiple-gene-deletion system for Streptococcus mutans
    • Banerjee, A., and I. Biswas. 2008. Markerless multiple-gene-deletion system for Streptococcus mutans. Appl. Environ. Microbiol. 74:2037-2042.
    • (2008) Appl. Environ. Microbiol. , vol.74 , pp. 2037-2042
    • Banerjee, A.1    Biswas, I.2
  • 7
    • 34548505486 scopus 로고    scopus 로고
    • Analysis of a ferric uptake regulator (Fur) mutant of Desulfovibrio vulgaris Hildenborough
    • Bender, K. S., et al. 2007. Analysis of a ferric uptake regulator (Fur) mutant of Desulfovibrio vulgaris Hildenborough. Appl. Environ. Microbiol. 73: 5389-5400.
    • (2007) Appl. Environ. Microbiol. , vol.73 , pp. 5389-5400
    • Bender, K.S.1
  • 8
    • 15444350252 scopus 로고    scopus 로고
    • The complete genome sequence of Escherichia coli K-12
    • Blattner, F. R., et al. 1997. The complete genome sequence of Escherichia coli K-12. Science 277:1453-1462.
    • (1997) Science , vol.277 , pp. 1453-1462
    • Blattner, F.R.1
  • 9
    • 0017184389 scopus 로고
    • A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
    • Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
    • (1976) Anal. Biochem. , vol.72 , pp. 248-254
    • Bradford, M.M.1
  • 10
    • 34948885665 scopus 로고    scopus 로고
    • Effects of Fis on Escherichia coli gene expression during different growth stages
    • Bradley, M. D., M. B. Beach, A. P. de Koning, T. S. Pratt, and R. Osuna. 2007. Effects of Fis on Escherichia coli gene expression during different growth stages. Microbiology 153:2922-2940.
    • (2007) Microbiology , vol.153 , pp. 2922-2940
    • Bradley, M.D.1    Beach, M.B.2    de Koning, A.P.3    Pratt, T.S.4    Osuna, R.5
  • 11
    • 0029824860 scopus 로고    scopus 로고
    • Direct selection of cloned DNA in Bacillus subtilis based on sucrose-induced lethality
    • Bramucci, M. G., and V. Nagarajan. 1996. Direct selection of cloned DNA in Bacillus subtilis based on sucrose-induced lethality. Appl. Environ. Microbiol. 62:3948-3953.
    • (1996) Appl. Environ. Microbiol. , vol.62 , pp. 3948-3953
    • Bramucci, M.G.1    Nagarajan, V.2
  • 12
    • 0019777576 scopus 로고
    • Growth of Desulfovibrio species on hydrogen and sulfate as sole energy-source
    • Brandis, A., and R. K. Thauer. 1981. Growth of Desulfovibrio species on hydrogen and sulfate as sole energy-source. J. Gen. Microbiol. 126:249-252.
    • (1981) J. Gen. Microbiol. , vol.126 , pp. 249-252
    • Brandis, A.1    Thauer, R.K.2
  • 13
    • 50849098825 scopus 로고    scopus 로고
    • eSGA: E. coli synthetic genetic array analysis
    • Butland, G., et al. 2008. eSGA: E. coli synthetic genetic array analysis. Nat. Methods 5:789-795.
    • (2008) Nat. Methods , vol.5 , pp. 789-795
    • Butland, G.1
  • 14
    • 13444283630 scopus 로고    scopus 로고
    • Interaction network containing conserved and essential protein complexes in Escherichia coli
    • Butland, G., et al. 2005. Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433:531-537.
    • (2005) Nature , vol.433 , pp. 531-537
    • Butland, G.1
  • 15
    • 47249101480 scopus 로고    scopus 로고
    • A defined transposon mutant library and its use in identifying motility genes in Vibrio cholerae
    • Cameron, D. E., J. M. Urbach, and J. J. Mekalanos. 2008. A defined transposon mutant library and its use in identifying motility genes in Vibrio cholerae. Proc. Natl. Acad. Sci. U. S. A. 105:8736-8741.
    • (2008) Proc. Natl. Acad. Sci. U. S. A. , vol.105 , pp. 8736-8741
    • Cameron, D.E.1    Urbach, J.M.2    Mekalanos, J.J.3
  • 16
    • 33644748620 scopus 로고    scopus 로고
    • Global analysis of heat shock response in Desulfovibrio vulgaris Hildenborough
    • Chhabra, S. R., et al. 2006. Global analysis of heat shock response in Desulfovibrio vulgaris Hildenborough. J. Bacteriol. 188:1817-1828.
    • (2006) J. Bacteriol. , vol.188 , pp. 1817-1828
    • Chhabra, S.R.1
  • 17
    • 79959592317 scopus 로고    scopus 로고
    • Towards a rigorous network of protein-protein interactions of the model sulfate reducer Desulfovibrio vulgaris Hildenborough
    • doi: 10.1371/journal.pone.0021470
    • Chhabra, S. R., et al. 2011. Towards a rigorous network of protein-protein interactions of the model sulfate reducer Desulfovibrio vulgaris Hildenborough. PLoS One 6:e21470. doi:10.1371/journal.pone.0021470.
    • (2011) PLoS One , vol.6
    • Chhabra, S.R.1
  • 18
    • 33747337245 scopus 로고    scopus 로고
    • Temporal transcriptomic analysis as Desulfovibrio vulgaris Hildenborough transitions into stationary phase during electron donor depletion
    • Clark, M. E., et al. 2006. Temporal transcriptomic analysis as Desulfovibrio vulgaris Hildenborough transitions into stationary phase during electron donor depletion. Appl. Environ. Microbiol. 72:5578-5588.
    • (2006) Appl. Environ. Microbiol , vol.72 , pp. 5578-5588
    • Clark, M.E.1
  • 19
    • 0034612342 scopus 로고    scopus 로고
    • One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
    • Datsenko, K. A., and B. L. Wanner. 2000. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U. S. A. 97:6640-6645.
    • (2000) Proc. Natl. Acad. Sci. U. S. A. , vol.97 , pp. 6640-6645
    • Datsenko, K.A.1    Wanner, B.L.2
  • 20
    • 42949142245 scopus 로고    scopus 로고
    • A complete collection of single-gene deletion mutants of Acinetobacter baylyi ADP1
    • de Berardinis, V., et al. 2008. A complete collection of single-gene deletion mutants of Acinetobacter baylyi ADP1. Mol. Syst. Biol. 4:174.
    • (2008) Mol. Syst. Biol. , vol.4 , pp. 174
    • de Berardinis, V.1
  • 21
    • 0024291348 scopus 로고
    • Integration host factor: a protein for all reasons
    • Friedman, D. I. 1988. Integration host factor: a protein for all reasons. Cell 55:545-554.
    • (1988) Cell , vol.55 , pp. 545-554
    • Friedman, D.I.1
  • 22
    • 33846504443 scopus 로고    scopus 로고
    • A comprehensive transposon mutant library of Francisella novicida, a bioweapon surrogate
    • Gallagher, L. A., et al. 2007. A comprehensive transposon mutant library of Francisella novicida, a bioweapon surrogate. Proc. Natl. Acad. Sci. U. S. A. 104:1009-1014.
    • (2007) Proc. Natl. Acad. Sci. U. S. A. , vol.104 , pp. 1009-1014
    • Gallagher, L.A.1
  • 23
    • 23644442434 scopus 로고    scopus 로고
    • Evolutionary plasticity of methionine biosynthesis
    • Gophna, U., E. Bapteste, W. F. Doolittle, D. Biran, and E. Z. Ron. 2005. Evolutionary plasticity of methionine biosynthesis. Gene 355:48-57.
    • (2005) Gene , vol.355 , pp. 48-57
    • Gophna, U.1    Bapteste, E.2    Doolittle, W.F.3    Biran, D.4    Ron, E.Z.5
  • 24
    • 32044474842 scopus 로고    scopus 로고
    • A method adapting microarray technology for signature-tagged mutagenesis of Desulfovibrio desulfuricans G20 and Shewanella oneidensis MR-1 in anaerobic sediment survival experiments
    • Groh, J. L., Q. Luo, J. D. Ballard, and L. R. Krumholz. 2005. A method adapting microarray technology for signature-tagged mutagenesis of Desulfovibrio desulfuricans G20 and Shewanella oneidensis MR-1 in anaerobic sediment survival experiments. Appl. Environ. Microbiol. 71:7064-7074.
    • (2005) Appl. Environ. Microbiol. , vol.71 , pp. 7064-7074
    • Groh, J.L.1    Luo, Q.2    Ballard, J.D.3    Krumholz, L.R.4
  • 25
    • 10744229659 scopus 로고    scopus 로고
    • Comprehensive transposon mutant library of Pseudomonas aeruginosa
    • Jacobs, M. A., et al. 2003. Comprehensive transposon mutant library of Pseudomonas aeruginosa. Proc. Natl. Acad. Sci. U. S. A. 100:14339-14344.
    • (2003) Proc. Natl. Acad. Sci. U. S. A. , vol.100 , pp. 14339-14344
    • Jacobs, M.A.1
  • 26
    • 73249117149 scopus 로고    scopus 로고
    • Development of a markerless genetic exchange system in Desulfovibrio vulgaris Hildenborough and its use in generating a strain with increased transformation efficiency
    • Keller, K. L., K. S. Bender, and J. D. Wall. 2009. Development of a markerless genetic exchange system in Desulfovibrio vulgaris Hildenborough and its use in generating a strain with increased transformation efficiency. Appl. Environ. Microbiol. 75:7682-7691.
    • (2009) Appl. Environ. Microbiol. , vol.75 , pp. 7682-7691
    • Keller, K.L.1    Bender, K.S.2    Wall, J.D.3
  • 27
    • 36749013034 scopus 로고    scopus 로고
    • Development of a markerless gene knock-out system for Mannheimia succiniciproducens using a temperature-sensitive plasmid
    • Kim, J. M., K. H. Lee, and S. Y. Lee. 2008. Development of a markerless gene knock-out system for Mannheimia succiniciproducens using a temperature-sensitive plasmid. FEMS Microbiol. Lett. 278:78-85.
    • (2008) FEMS Microbiol. Lett. , vol.278 , pp. 78-85
    • Kim, J.M.1    Lee, K.H.2    Lee, S.Y.3
  • 28
    • 26844580763 scopus 로고    scopus 로고
    • Development of a method for markerless genetic exchange in Enterococcus faecalis and its use in construction of a srtA mutant
    • Kristich, C. J., D. A. Manias, and G. M. Dunny. 2005. Development of a method for markerless genetic exchange in Enterococcus faecalis and its use in construction of a srtA mutant. Appl. Environ. Microbiol. 71:5837-5849.
    • (2005) Appl. Environ. Microbiol. , vol.71 , pp. 5837-5849
    • Kristich, C.J.1    Manias, D.A.2    Dunny, G.M.3
  • 29
    • 33847608289 scopus 로고    scopus 로고
    • Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC
    • Li, M. Z., and S. J. Elledge. 2007. Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC. Nat. Methods 4:251-256.
    • (2007) Nat. Methods , vol.4 , pp. 251-256
    • Li, M.Z.1    Elledge, S.J.2
  • 30
    • 33644504829 scopus 로고    scopus 로고
    • An ordered, nonredundant library of Pseudomonas aeruginosa strain PA14 transposon insertion mutants
    • Liberati, N. T., et al. 2006. An ordered, nonredundant library of Pseudomonas aeruginosa strain PA14 transposon insertion mutants. Proc. Natl. Acad. Sci. U. S. A. 103:2833-2838.
    • (2006) Proc. Natl. Acad. Sci. U. S. A. , vol.103 , pp. 2833-2838
    • Liberati, N.T.1
  • 31
    • 77957963526 scopus 로고    scopus 로고
    • Identification of conditionally essential genes for growth of Pseudomonas putida KT2440 on minimal medium through the screening of a genome-wide mutant library
    • Molina-Henares, M. A., et al. 2010. Identification of conditionally essential genes for growth of Pseudomonas putida KT2440 on minimal medium through the screening of a genome-wide mutant library. Environ. Microbiol. 12:1468-1485.
    • (2010) Environ. Microbiol. , vol.12 , pp. 1468-1485
    • Molina-Henares, M.A.1
  • 32
    • 33744776637 scopus 로고    scopus 로고
    • Salt stress in Desulfovibrio vulgaris Hildenborough: an integrated genomics approach
    • Mukhopadhyay, A., et al. 2006. Salt stress in Desulfovibrio vulgaris Hildenborough: an integrated genomics approach. J. Bacteriol. 188:4068-4078.
    • (2006) J. Bacteriol. , vol.188 , pp. 4068-4078
    • Mukhopadhyay, A.1
  • 33
    • 77954648994 scopus 로고    scopus 로고
    • Development of SNAP-tag-mediated live cell labeling as an alternative to GFP in Porphyromonas gingivalis
    • Nicolle, O., et al. 2010. Development of SNAP-tag-mediated live cell labeling as an alternative to GFP in Porphyromonas gingivalis. FEMS Immunol. Med. Microbiol. 59:357-363.
    • (2010) FEMS Immunol. Med. Microbiol. , vol.59 , pp. 357-363
    • Nicolle, O.1
  • 34
    • 77954095162 scopus 로고    scopus 로고
    • Systematic screens of a Candida albicans homozygous deletion library decouple morphogenetic switching and pathogenicity
    • Noble, S. M., S. French, L. A. Kohn, V. Chen, and A. D. Johnson. 2010. Systematic screens of a Candida albicans homozygous deletion library decouple morphogenetic switching and pathogenicity. Nat. Genet. 42:590-598.
    • (2010) Nat. Genet. , vol.42 , pp. 590-598
    • Noble, S.M.1    French, S.2    Kohn, L.A.3    Chen, V.4    Johnson, A.D.5
  • 35
    • 33750380250 scopus 로고    scopus 로고
    • Suicide vectors for antibiotic marker exchange and rapid generation of multiple knockout mutants by allelic exchange in Gram-negative bacteria
    • Ortiz-Martin, I., A. P. Macho, L. Lambersten, C. Ramos, and C. R. Beuzon. 2006. Suicide vectors for antibiotic marker exchange and rapid generation of multiple knockout mutants by allelic exchange in Gram-negative bacteria. J. Microbiol. Methods 67:395-407.
    • (2006) J. Microbiol. Methods , vol.67 , pp. 395-407
    • Ortiz-Martin, I.1    Macho, A.P.2    Lambersten, L.3    Ramos, C.4    Beuzon, C.R.5
  • 36
    • 33644808569 scopus 로고    scopus 로고
    • SNAP-tag mediated live cell labeling as an alternative to GFP in anaerobic organisms
    • 812
    • Regoes, A., and A. B. Hehl. 2005. SNAP-tag mediated live cell labeling as an alternative to GFP in anaerobic organisms. Biotechniques 39:809-810, 812.
    • (2005) Biotechniques , vol.39 , pp. 809-810
    • Regoes, A.1    Hehl, A.B.2
  • 37
    • 0018405881 scopus 로고
    • E. coli DNA binding protein HU forms nucleosomelike structure with circular double-stranded DNA
    • Rouviere-Yaniv, J., M. Yaniv, and J. E. Germond. 1979. E. coli DNA binding protein HU forms nucleosomelike structure with circular double-stranded DNA. Cell 17:265-274.
    • (1979) Cell , vol.17 , pp. 265-274
    • Rouviere-Yaniv, J.1    Yaniv, M.2    Germond, J.E.3
  • 38
    • 37449020577 scopus 로고    scopus 로고
    • Response of Desulfovibrio vulgaris to alkaline stress
    • Stolyar, S., et al. 2007. Response of Desulfovibrio vulgaris to alkaline stress. J. Bacteriol. 189:8944-8952.
    • (2007) J. Bacteriol. , vol.189 , pp. 8944-8952
    • Stolyar, S.1
  • 39
    • 77955102352 scopus 로고    scopus 로고
    • Quantifying E. coli proteome and transcriptome with single-molecule sensitivity in single cells
    • Taniguchi, Y., et al. 2010. Quantifying E. coli proteome and transcriptome with single-molecule sensitivity in single cells. Science 329:533-538.
    • (2010) Science , vol.329 , pp. 533-538
    • Taniguchi, Y.1
  • 40
    • 34547786556 scopus 로고    scopus 로고
    • Multi-copy plasmid modification with phage lambda Red recombineering
    • Thomason, L. C., N. Costantino, D. V. Shaw, and D. L. Court. 2007. Multi-copy plasmid modification with phage lambda Red recombineering. Plasmid 58:148-158.
    • (2007) Plasmid , vol.58 , pp. 148-158
    • Thomason, L.C.1    Costantino, N.2    Shaw, D.V.3    Court, D.L.4
  • 41
    • 62449230949 scopus 로고    scopus 로고
    • Modifying bacteriophage lambda with recombineering
    • Thomason, L. C., A. B. Oppenheim, and D. L. Court. 2009. Modifying bacteriophage lambda with recombineering. Methods Mol. Biol. 501:239-251.
    • (2009) Methods Mol. Biol. , vol.501 , pp. 239-251
    • Thomason, L.C.1    Oppenheim, A.B.2    Court, D.L.3
  • 42
    • 0027514035 scopus 로고
    • Topology and subcellular localization of FtsH protein in Escherichia coli
    • Tomoyasu, T., et al. 1993. Topology and subcellular localization of FtsH protein in Escherichia coli. J. Bacteriol. 175:1352-1357.
    • (1993) J. Bacteriol. , vol.175 , pp. 1352-1357
    • Tomoyasu, T.1
  • 43
    • 50849125711 scopus 로고    scopus 로고
    • High-throughput, quantitative analyses of genetic interactions in E. coli
    • Typas, A., et al. 2008. High-throughput, quantitative analyses of genetic interactions in E. coli. Nat. Methods 5:781-787.
    • (2008) Nat. Methods , vol.5 , pp. 781-787
    • Typas, A.1
  • 44
    • 0020442864 scopus 로고
    • The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers
    • Vieira, J., and J. Messing. 1982. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259-268.
    • (1982) Gene , vol.19 , pp. 259-268
    • Vieira, J.1    Messing, J.2
  • 46
    • 66049157868 scopus 로고    scopus 로고
    • Quantitative genome-scale analysis of protein localization in an asymmetric bacterium
    • Werner, J. N., et al. 2009. Quantitative genome-scale analysis of protein localization in an asymmetric bacterium. Proc. Natl. Acad. Sci. U. S. A. 106:7858-7863.
    • (2009) Proc. Natl. Acad. Sci. U. S. A. , vol.106 , pp. 7858-7863
    • Werner, J.N.1
  • 47
    • 0034705144 scopus 로고    scopus 로고
    • An efficient recombination system for chromosome engineering in Escherichia coli
    • Yu, D., et al. 2000. An efficient recombination system for chromosome engineering in Escherichia coli. Proc. Natl. Acad. Sci. U. S. A. 97:5978-5983.
    • (2000) Proc. Natl. Acad. Sci. U. S. A. , vol.97 , pp. 5978-5983
    • Yu, D.1
  • 48
    • 0038472057 scopus 로고    scopus 로고
    • Recombineering with overlapping single-stranded DNA oligonucleotides: testing a recombination intermediate
    • Yu, D., J. A. Sawitzke, H. Ellis, and D. L. Court. 2003. Recombineering with overlapping single-stranded DNA oligonucleotides: testing a recombination intermediate. Proc. Natl. Acad. Sci. U. S. A. 100:7207-7212.
    • (2003) Proc. Natl. Acad. Sci. U. S. A. , vol.100 , pp. 7207-7212
    • Yu, D.1    Sawitzke, J.A.2    Ellis, H.3    Court, D.L.4
  • 49
    • 77954199597 scopus 로고    scopus 로고
    • PSORTb 3.0: improved protein subcellular localization prediction with refined localization subcategories and predictive capabilities for all prokaryotes
    • Yu, N. Y., et al. 2010. PSORTb 3.0: improved protein subcellular localization prediction with refined localization subcategories and predictive capabilities for all prokaryotes. Bioinformatics 26:1608-1615.
    • (2010) Bioinformatics , vol.26 , pp. 1608-1615
    • Yu, N.Y.1
  • 50
    • 77955964940 scopus 로고    scopus 로고
    • Effect of the deletion of qmoABC and the promoter-distal gene encoding a hypothetical protein on sulfate reduction in Desulfovibrio vulgaris Hildenborough
    • Zane, G. M., H. C. Yen, and J. D. Wall. 2010. Effect of the deletion of qmoABC and the promoter-distal gene encoding a hypothetical protein on sulfate reduction in Desulfovibrio vulgaris Hildenborough. Appl. Environ. Microbiol. 76:5500-5509.
    • (2010) Appl. Environ. Microbiol. , vol.76 , pp. 5500-5509
    • Zane, G.M.1    Yen, H.C.2    Wall, J.D.3
  • 51
    • 4444281106 scopus 로고    scopus 로고
    • Sequential peptide affinity (SPA) system for the identification of mammalian and bacterial protein complexes
    • Zeghouf, M., et al. 2004. Sequential peptide affinity (SPA) system for the identification of mammalian and bacterial protein complexes. J. Proteome Res. 3:463-468.
    • (2004) J. Proteome Res. , vol.3 , pp. 463-468
    • Zeghouf, M.1


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