Markerless multiple-gene-deletion system for Streptococcus mutans

Applied and Environmental Microbiology

Volumn 74, Issue 7, 2008, Pages 2037-2042

  Banerjee, Anirban ^a   Biswas, Indranil ^a,b  

^a UNIVERSITY OF SOUTH DAKOTA   (United States)

^b UNIVERSITY OF KANSAS SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; MUTAGENESIS; PH EFFECTS; PROTEINS;

ETIOLOGICAL AGENT; STREPTOCOCCUS MUTANS;

GENES;

CRE RECOMBINASE; VIRULENCE FACTOR;

BACTERIUM; CHROMOSOME; DENTITION; GENE EXPRESSION; MUTATION; PH; PROTEIN; RECOMBINATION; VIRULENCE;

ARTICLE; BACTERIAL CHROMOSOME; BACTERIAL GENETICS; BACTERIAL VIRULENCE; GENE DELETION; GENE INACTIVATION; GENE LOCUS; GENETIC RECOMBINATION; NONHUMAN; NUCLEOTIDE SEQUENCE; OXIDATIVE STRESS; PH; POINT MUTATION; PROTEIN PROCESSING; STREPTOCOCCUS MUTANS;

ATTACHMENT SITES, MICROBIOLOGICAL; GENE DELETION; GENE EXPRESSION REGULATION, BACTERIAL; GENES, BACTERIAL; GENETIC MARKERS; GENOME, BACTERIAL; INTEGRASES; STREPTOCOCCUS MUTANS;

STREPTOCOCCUS MUTANS;

EID: 42049092059     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.02346-07     Document Type: Article

References (49)

1. Abremski, K., and R. Hoess. 1984. Bacteriophage P1 site-specific recombination, purification and properties of the Cre recombinase protein. J. Biol. Chem. 259:1509-1514.
2. Ahn, S. J., J. A. Lemos, and R. A. Burne. 2005. Role of HtrA in growth and competence of Streptococcus mutans UA159. J. Bacteriol. 187:3028-3038.
3. Ajdic, D., W. M. McShan, R. E. McLaughlin, G. Savic, J. Chang, M. B. Carson, C. Primeaux, R. Tian, S. Kenton, H. Jia, S. Lin, Y. Qian, S. Li, H. Zhu, F. Najar, H. Lai, J. White, B. A. Roe, and J. J. Ferretti. 2002. Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen. Proc. Natl. Acad. Sci. USA 99:14434-14439.
4. Albert, H., E. C. Dale, E. Lee, and D. W. Ow. 1995. Site-specific integration of DNA into wild-type and mutant lox sites placed in the plant genome. Plant J. 7:649-659.
5. Araki, K., M. Araki, and K. Yamamura. 2002. Site-directed integration of the cre gene mediated by Cre recombinase using a combination of mutant lox sites. Nucleic Acids Res. 30:e103.
6. Atlagic, D., A. O. Kilic, and L. Tao. 2006. Unmarked gene deletion mutagenesis of gtfB and gtfC in Streptococcus mutans using a targeted hit-and-run strategy with a thermosensitive plasmid. Oral Microbiol. Immunol. 21:132-135.
7. Belli, W. A., and R. E. Marquis. 1991. Adaptation of Streptococcus mutans and Enterococcus hirae to acid stress in continuous culture. Appl. Environ. Microbiol. 57:1134-1138.
8. Biswas, I., L. Drake, D. Erkina, and S. Biswas. 2008. Involvement of sensor kinases in the stress tolerance response of Streptococcus mutans. J. Bacteriol. 190:68-77.
9. Biswas, I., L. Drake, S. Johnson, and D. Thielen. 2007. Unmarked gene modification in Streptococcus mutans by a cotransformation strategy with a thermosensitive plasmid. BioTechniques 42:487-490.
10. Biswas, I., A. Gross, S. D. Ehrlich, and E. Maguin. 1993. High-efficiency gene inactivation and replacement system for gram-positive bacteria. J. Bacteriol. 175:3628-3635.
11. Biswas, S., and I. Biswas. 2005. Role of HtrA in surface protein expression and biofilm formation by Streptococcus mutans. Infect. Immun. 73:6923-6934.
12. Campo, N., M. L. Daveran-Mingot, K. Leenhouts, P. Ritzenthaler, and P. Le Bourgeois. 2002. Cre-loxP recombination system for large genome rearrangements in Lactococcus lactis. Appl. Environ. Microbiol. 68:2359-2367.
13. Diaz-Torres, M. L., and R. R. Russell. 2001. HtrA protease and processing of extracellular proteins of Streptococcus mutans. FEMS Microbiol. Lett. 204:23-28.
14. Frees, D., and H. Ingmer. 1999. ClpP participates in the degradation of misfolded protein in Lactococcus lactis. Mol. Microbiol. 31:79-87.
15. Gilbertson, L. 2003. Cre-lox recombination: Cre-ative tools for plant biotechnology. Trends Biotechnol. 21:550-555.
16. Gutierrez, J. A., P. J. Crowley, D. P. Brown, J. D. Hillman, P. Youngman, and A. S. Bleiweis. 1996. Insertional mutagenesis and recovery of interrupted genes of Streptococcus mutans by using transposon Tn977: preliminary characterization of mutants displaying acid sensitivity and nutritional requirements. J. Bacteriol. 178:4166-4175.
17. Hamilton, I. R., and N. D. Buckley. 1991. Adaptation by Streptococcus mutans to acid tolerance. Oral Microbiol. Immunol. 6:65-71.
18. Hamilton, I. R., and G. Svensater. 1998. Acid-regulated proteins induced by Streptococcus mutans and other oral bacteria during acid shock. Oral Microbiol. Immunol. 13:292-300.
19. Hoess, R. H., M. Ziese, and N. Sternberg. 1982. P1 site-specific recombination: nucleotide sequence of the recombining sites. Proc. Natl. Acad. Sci. USA 79:3398-3402.
20. Husmann, L. K., J. R. Scott, G. Lindahl, and L. Stenberg. 1995. Expression of protein Arp, a member of the M protein family, is not sufficient to inhibit phagocytosis of Streptococcus pyogenes. Infect. Immun. 63:345-348.
21. Iannelli, F., and G. Pozzi. 2004. Method for introducing specific and unmarked mutations into the chromosome of Streptococcus pneumoniae. Mol. Biotechnol. 26:81-86.
22. Kuramitsu, H. K. 2003. Molecular genetic analysis of the virulence of oral bacterial pathogens: an historical perspective. Crit. Rev. Oral Biol. Med. 14:331-344.
23. Kuramitsu, H. K. 1987. Utilization of a mini-mu transposon to construct defined mutants in Streptococcus mutans. Mol. Microbiol. 1:229-232.
24. Lambert, J. M., R. S. Bongers, and M. Kleerebezem. 2007. Cre-lox-based system for multiple gene deletions and selectable-marker removal in Lactobacillus plantarum. Appl. Environ. Microbiol. 73:1126-1135.
25. Lane, M. A., K. W. Bayles, and R. E. Yasbin. 1991. Identification and initial characterization of glucose-repressible promoters of Streptococcus mutans. Gene 100:225-229.
26. Langer, S. J., A. P. Ghafoori, M. Byrd, and L. Leinwand. 2002. A genetic screen identifies novel non-compatible loxP sites. Nucleic Acids Res. 30: 3067-3077.
27. Lau, P. C., C. K. Sung, J. H. Lee, D. A. Morrison, and D. G. Cvitkovitch. 2002. PCR ligation mutagenesis in transformable streptococci: application and efficiency. J. Microbiol. Methods 49:193-205.
28. Lemos, J. A., and R. A. Burne. 2002. Regulation and physiological significance of ClpC and ClpP in Streptococcus mutans. J. Bacteriol. 184:6357-6366.
29. Loesche, W. J. 1986. Role of Streptococcus mutans in human dental decay. Microbiol. Rev. 50:353-380.
30. Maguin, E., P. Duwat, T. Hege, D. Ehrlich, and A. Gross. 1992. New thermosensitive plasmid for gram-positive bacteria. J. Bacteriol. 174:5633-5638.
31. Merritt, J., P. Tsang, L. Zheng, W. Shi, and F. Qi. 2007. Construction of a counterselection-based in-frame deletion system for genetic studies of Streptococcus mutans. Oral Microbiol. Immunol. 22:95-102.
32. Min Kim, J., J. Young Choi, M. Sun Kim, and S. Chang Kim. 2004. In vivo excision and amplification of large human genomic segments using the Cre/loxP-and large T antigen/SV40 ori-mediated machinery. J. Biotechnol. 110: 227-233.
33. Palmeros, B., J. Wild, W. Szybalski, S. Le Borgne, G. Hernandez-Chavez, G. Gosset, F. Valle, and F. Bolivar. 2000. A family of removable cassettes designed to obtain antibiotic-resistance-free genomic modifications of Escherichia coli and other bacteria. Gene 247:255-264.
34. Perez-Casal, J., M. G. Caparon, and J. R. Scott. 1991. Mry, a trans-acting positive regulator of the M protein gene of Streptococcus pyogenes with similarity to the receptor proteins of two-component regulatory systems. J. Bacteriol. 173:2617-2624.
35. Pomerantsev, A. P., K. V. Kalnin, M. Osorio, and S. H. Leppla. 2003. Phosphatidylcholine-specific phospholipasc C and sphingomyelinase activities in bacteria of the Bacillus cereus group. Infect. Immun. 71:6591-6606.
36. Pomerantsev, A. P., O. M. Pomerantseva, and S. H. Leppla. 2004. A spontaneous translational fusion of Bacillus cereus PlcR and PapR activates transcription of PlcR-dependent genes in Bacillus anthracis via binding with a specific palindromic sequence. Infect. Immun. 72:5814-5823.
37. Pomerantsev, A. P., R. Sitaraman, C. R. Galloway, V. Kivovich, and S. H. Leppla. 2006. Genome engineering in Bacillus anthracis using Cre recombinase. Infect. Immun. 74:682-693.
38. Pritzlaff, C. A., J. C. Chang, S. P. Kuo, G. S. Tamura, C. E. Rubens, and V. Nizet. 2001. Genetic basis for the beta-haemolytic/cytolytic activity of group B Streptococcus. Mol. Microbiol. 39:236-247.
39. Procino, J. K., L. Marri, G. D. Shockman, and L. Daneo-Moore. 1988. Tn916 insertional inactivation of multiple genes on the chromosome of Streptococcus mutans GS-5. Infect. Immun. 56:2866-2870.
40. Sauer, B. 1987. Functional expression of the cre-lox site-specific recombination system in the yeast Saccharomyces cerevisiae. Mol. Cell. Biol. 7:2087-2096.
41. Sauer, B. 1998. Inducible gene targeting in mice using the Cre/lox system. Methods 14:381-392.
42. Sternberg, N. 1981. Bacteriophage P1 site-specific recombination. III. Strand exchange during recombination at lox sites. J. Mol. Biol. 150:603-608.
43. Sung, C. K., H. Li, J. P. Claverys, and D. A. Morrison. 2001. An rpsL cassette, Janus, for gene replacement through negative selection in Streptococcus pneumoniae. Appl. Environ. Microbiol. 67:5190-5196.
44. Suzuki, N., M. Inui, and H. Yukawa. 2007. Site-directed integration system using a combination of mutant lox sites for Corynebacterium glutamicum. Appl. Microbiol. Biotechnol. 77:871-878.
45. Tao, L., D. J. LeBlanc, and J. J. Ferretti. 1992. Novel streptococcal-integration shuttle vectors for gene cloning and inactivation. Gene 120:105-110.
46. Tao, L., and J. M. Tanzer. 2002. Novel sucrose-dependent adhesion cofactors in Streptococcus mutans. J. Dent. Res. 81:505-510.
47. Tsang, P., J. Merritt, T. Nguyen, W. Shi, and F. Qi. 2005. Identification of genes associated with mutacin I production in Streptococcus mutans using random insertional mutagenesis. Microbiology 151:3947-3955.
48. VanBogelen, R. A., and F. C. Neidhardt. 1990. Ribosomes as sensors of heat and cold shock in Escherichia coli. Proc. Natl. Acad. Sci. USA 87:5589-5593.
49. Vergunst, A. C., and P. J. Hooykaas. 1998. Cre/lox-mediated site-specific integration of Agrobacterium T-DNA in Arabidopsis thaliana by transient expression of cre. Plant Mol. Biol 38:393-406.