Development of SNAP-tag-mediated live cell labeling as an alternative to GFP in Porphyromonas gingivalis

FEMS Immunology and Medical Microbiology

Volumn 59, Issue 3, 2010, Pages 357-363

  Nicolle, Ophélie ^a   Rouillon, Astrid ^a   Guyodo, Helene ^a   Tamanai Shacoori, Zohreh ^a   Chandad, Fatiha ^b   Meuric, Vincent ^a,c   Bonnaure Mallet, Martine ^a,c  

^a UNIVERSITÉ EUROPÉENNE DE BRETAGNE   (France)

^b UNIVERSITÉ LAVAL   (Canada)

^c RENNES UNIVERSITY HOSPITAL   (France)

Author keywords

biofilm; confocal laser scanning microscopy (CLSM); green fluorescent protein (GFP); Porphyromonas gingivalis; SNAP tag; Streptococcus gordonii

Indexed keywords

6 O ALKYLGUANINE DNA ALKYLTRANSFERASE; 6 O BENZYLGUANINE; BACTERIAL PROTEIN; GREEN FLUORESCENT PROTEIN; PROTEIN SNAP26; UNCLASSIFIED DRUG; METHYLATED DNA PROTEIN CYSTEINE METHYLTRANSFERASE; RECOMBINANT PROTEIN;

ARTICLE; BIOFILM; CELL LABELING; DNA REPAIR; ENZYME MULTIPLIED IMMUNOASSAY TECHNIQUE; FLUORESCENCE ANALYSIS; MOUTH FLORA; NONHUMAN; OXYGEN CONSUMPTION; PORPHYROMONAS GINGIVALIS; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN BINDING; PROTEIN EXPRESSION; SCANNING ELECTRON MICROSCOPY; SEQUENCE ANALYSIS; STREPTOCOCCUS GORDONII; ANAEROBIC GROWTH; CONFOCAL MICROSCOPY; FLOW CYTOMETRY; FLUORESCENCE; GENE EXPRESSION; GENETICS; GROWTH, DEVELOPMENT AND AGING; HUMAN; METABOLISM; MICROBIAL GENETICS; MICROBIOLOGICAL EXAMINATION; MOLECULAR BIOLOGY; PLASMID; PROCEDURES; REPORTER GENE; STAINING;

ANAEROBIOSIS; BACTERIOLOGICAL TECHNIQUES; BIOFILMS; FLOW CYTOMETRY; FLUORESCENCE; GENE EXPRESSION; GENES, REPORTER; GENETICS, MICROBIAL; GREEN FLUORESCENT PROTEINS; HUMANS; MICROSCOPY, CONFOCAL; MOLECULAR BIOLOGY; O(6)-METHYLGUANINE-DNA METHYLTRANSFERASE; PLASMIDS; PORPHYROMONAS GINGIVALIS; PROMOTER REGIONS, GENETIC; RECOMBINANT PROTEINS; STAINING AND LABELING; STREPTOCOCCUS GORDONII;

EID: 77954648994     PISSN: 09288244     EISSN: 1574695X     Source Type: Journal    
DOI: 10.1111/j.1574-695X.2010.00681.x     Document Type: Article

References (26)

1. Belanger M, Rodrigues P Progulske-Fox A (2007) Genetic manipulation of Porphyromonas gingivalis. Curr Protoc Microbiol 13C2 : 1 3.
2. Chalfie M, Tu Y, Euskirchen G, Ward WW Prasher DC (1994) Green fluorescent protein as a marker for gene expression. Science 263 : 802 805.
3. Christensen BB, Haagensen JA, Heydorn A Molin S (2002) Metabolic commensalism and competition in a two-species microbial consortium. Appl Environ Microb 68 : 2495 2502.
4. Drenkard E Ausubel FM (2002) Pseudomonas biofilm formation and antibiotic resistance are linked to phenotypic variation. Nature 416 : 740 743.
5. Errampalli D, Leung K, Cassidy MB, Kostrzynska M, Blears M, Lee H Trevors JT (1999) Applications of the green fluorescent protein as a molecular marker in environmental microorganisms. J Microbiol Meth 35 : 187 199.
6. Hansen MC, Palmer RJ Jr., Udsen C, White DC Molin S (2001) Assessment of GFP fluorescence in cells of Streptococcus gordonii under conditions of low pH and low oxygen concentration. Microbiology 147 : 1383 1391. (Pubitemid 32454655)
7. Hentzer M, Riedel K, Rasmussen TB et al 2002) Inhibition of quorum sensing in Pseudomonas aeruginosa biofilm bacteria by a halogenated furanone compound. Microbiology 148 : 87 102.
8. Heydorn A, Ersboll B, Kato J et al 2002) Statistical analysis of Pseudomonas aeruginosa biofilm development: impact of mutations in genes involved in twitching motility, cell-to-cell signaling, and stationary-phase sigma factor expression. Appl Environ Microb 68 : 2008 2017.
9. Keppler A, Gendreizig S, Gronemeyer T, Pick H, Vogel H Johnsson K (2003) A general method for the covalent labeling of fusion proteins with small molecules in vivo. Nat Biotechnol 21 : 86 89.
10. Keppler A, Arrivoli C, Sironi L Ellenberg J (2006) Fluorophores for live cell imaging of AGT fusion proteins across the visible spectrum. Biotechniques 41 : 167 170.
11. Kindermann M, George N, Johnsson N Johnsson K (2003) Covalent and selective immobilization of fusion proteins. J Am Chem Soc 125 : 7810 7811.
12. Klausen M, Aaes-Jorgensen A, Molin S Tolker-Nielsen T (2003) Involvement of bacterial migration in the development of complex multicellular structures in Pseudomonas aeruginosa biofilms. Mol Microbiol 50 : 61 68.
13. Kuboniwa M, Tribble GD, James CE et al 2006) Streptococcus gordonii utilizes several distinct gene functions to recruit Porphyromonas gingivalis into a mixed community. Mol Microbiol 60 : 121 139.
14. Kumagai Y, Yajima A Konishi K (2003) Peptidase activity of dipeptidyl aminopeptidase IV produced by Porphyromonas gingivalis is important but not sufficient for virulence. Microbiol Immunol 47 : 735 743.
15. Lamont RJ Jenkinson HF (1998) Life below the gum line: pathogenic mechanisms of Porphyromonas gingivalis. Microbiol Mol Biol R 62 : 1244 1263.
16. Lawrence JR Neu TR (1999) Confocal laser scanning microscopy for analysis of microbial biofilms. Method Enzymol 310 : 131 144.
17. Liu Y, Abaibou H Fletcher HM (2000) Development of a noninvasive reporter system for gene expression in Porphyromonas gingivalis. Plasmid 44 : 250 261.
18. Neu TR, Kuhlicke U Lawrence JR (2002) Assessment of fluorochromes for two-photon laser scanning microscopy of biofilms. Appl Environ Microb 68 : 901 909.
19. Palmer RJ Jr. Sternberg C (1999) Modern microscopy in biofilm research: confocal microscopy and other approaches. Curr Opin Biotech 10 : 263 268.
20. Purevdorj B, Costerton JW Stoodley P (2002) Influence of hydrodynamics and cell signaling on the structure and behavior of Pseudomonas aeruginosa biofilms. Appl Environ Microb 68 : 4457 4464.
21. Regoes A Hehl AB (2005) SNAP-tag mediated live cell labeling as an alternative to GFP in anaerobic organisms. Biotechniques 39 : 809 810.
22. Rosan B Lamont RJ (2000) Dental plaque formation. Microbes Infect 2 : 1599 1607.
23. Takahashi Y, Kato D, Hamada N, Yoshimoto H Umemoto T (1999) Transformation and expression of a cloned fimA gene in Porphyromonas gingivalis. Infect Immun 67 : 2013 2018.
24. Tirat A, Freuler F, Stettler T, Mayr LM Leder L (2006) Evaluation of two novel tag-based labelling technologies for site-specific modification of proteins. Int J Biol Macromol 39 : 66 76.
25. Tolker-Nielsen T, Brinch UC, Ragas PC, Andersen JB, Jacobsen CS Molin S (2000) Development and dynamics of Pseudomonas sp. biofilms. J Bacteriol 182 : 6482 6489.
26. Tsien RY (1998) The green fluorescent protein. Annu Rev Biochem 67 : 509 544.