A protocol for in situ enzyme assays to assess the differentiation of human intestinal Caco-2 cells

Toxicology in Vitro

Volumn 26, Issue 8, 2012, Pages 1247-1251

  Ferruzza, Simonetta ^a   Rossi, Carlotta ^a   Scarino, Maria Laura ^a   Sambuy, Yula ^a  

^a ISTITUTO NAZIONALE DELLA NUTRIZIONE   (Italy)

Author keywords

Alanyl aminopeptidase; Alkaline phosphatase; Enterocytes; Sucrase

Indexed keywords

4 NITROPHENYL PHOSPHATE; ALANINE 4 NITROANILIDE; ALKALINE PHOSPHATASE; AMINO ACID; GLUCOSE OXIDASE; HORSERADISH PEROXIDASE; MICROSOMAL AMINOPEPTIDASE; POLYCARBONATE; RED FLUORESCENT PROTEIN; RESORUFIN; SUCRASE; UNCLASSIFIED DRUG; ALANINE-4-NITROANILIDE; ANILINE DERIVATIVE; NITROPHENOL; NITROPHENYL PHOSPHATE; ORGANOPHOSPHORUS COMPOUND; POLYCARBOXYLATE CEMENT;

APICAL MEMBRANE; ARTICLE; CACO-2 CELL LINE; CELL COMPARTMENTALIZATION; CELL DENSITY; CELL DIFFERENTIATION; CELL FREE SYSTEM; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME MECHANISM; ENZYME SUBSTRATE; FLUOROMETRY; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; IN VITRO STUDY; LIVE CELL IMAGING; PROTEIN ANALYSIS; REPRODUCIBILITY; CELL STRAIN CACO 2; CULTURE TECHNIQUE; ENZYMOLOGY; FILTRATION; INTESTINE CELL; METABOLISM; PHYSIOLOGY; TIME;

ALKALINE PHOSPHATASE; ANILINE COMPOUNDS; ANTIGENS, CD13; CACO-2 CELLS; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; ENTEROCYTES; FILTRATION; FLUOROMETRY; HUMANS; NITROPHENOLS; ORGANOPHOSPHORUS COMPOUNDS; POLYCARBOXYLATE CEMENT; REPRODUCIBILITY OF RESULTS; SUCRASE; TIME FACTORS;

EID: 82955246605     PISSN: 08872333     EISSN: 18793177     Source Type: Journal    
DOI: 10.1016/j.tiv.2011.11.007     Document Type: Article

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