An inter-laboratory study to evaluate the effects of medium composition on the differentiation and barrier function of Caco-2 cell lines

ATLA Alternatives to Laboratory Animals

Volumn 33, Issue 6, 2005, Pages 603-618

  Zucco, Flavia ^a   Batto, Anne Françoise ^b   Bises, Giovanna ^c   Chambaz, Jean ^b   Chiusolo, Arianna ^d   Consalvo, Rosa ^e   Cross, Heide ^c   Dal Negro, Gianni ^d   De Angelis, Isabella ^f   Fabre, Gérard ^g   Guillou, François ^g   Hoffman, Sebastian ^h   Laplanche, Loïc ^g   Morel, Etienne ^b   Pinçon Raymond, Martine ^b   Prieto, Pilar ^h   Turco, Laura ^f   Ranaldi, Giulia ^e   Rousset, Monique ^b   Sambuy, Yula ^e   Scarino, Maria Laura ^e   Torreilles, François ^g   Stammati, Annalaura ^f   more..

^a CNR   (Italy)

^b INSERM   (France)

^c MEDICAL UNIVERSITY OF VIENNA   (Austria)

^d GSK   (Italy)

^e ISTITUTO NAZIONALE DELLA NUTRIZIONE   (Italy)

^f ISTITUTO SUPERIORE DI SANITÀ   (Italy)

^g SANOFI   (France)

^h JOINT RESEARCH CENTRE   (Italy)

Author keywords

Barrier integrity; Caco 2; Defined medium; Epithelial differentiation; Permeability; Protocol standardisation

Indexed keywords

ALKALINE PHOSPHATASE; INSULIN; LIPID; SELENIUM; TRANSFERRIN;

ARTICLE; CELL CULTURE; CELL DIFFERENTIATION; CELL STRAIN CACO 2; CELL STRUCTURE; CONTROLLED STUDY; CULTURE MEDIUM; ELECTRIC RESISTANCE; ENZYME ACTIVITY; EPITHELIUM; HUMAN; HUMAN CELL; MEMBRANE PERMEABILITY; PRIORITY JOURNAL; REPRODUCIBILITY; TRANSEPITHELIAL ELECTRIC RESISTANCE;

EID: 31444436705     PISSN: 02611929     EISSN: None     Source Type: Journal    
DOI: 10.1177/026119290503300618     Document Type: Article

References (37)

1. Anon. (2001). White paper on a Strategy for a Future Chemicals Policy. COM(2001) 88 final. 32pp. Brussels, Belgium: Commission of the European Communities.
2. Anon. (2003). Directive 2003/15/EC of the European Parliament and of the Council of 27 February 2003 amending Council Directive 76/768/EEC on the approximations of the laws of the Member States relating to cosmetic products. Official Journal of the European Union L66, 26-35.
3. Fogh, J., Fogh, J.M. & Orfeo, T. (1977). One hundred and twenty seven cultured human tumour cell lines producing tumours in nude mice. Journal of the National Cancer Institute 59, 221-226.
4. Pinto, M., Robine-Leon, S., Appay, M.D., Kedinger, M., Tradou, N., Dussaulx, E., Lacroix, B., Simon-Assman, P., Haffen, K., Fogh, J. & Zweibaum, A. (1983). Enterocyte-like differentiation and polarization of the human colon carcinoma cell line Caco-2 in culture. Biology of the Cell 47, 323-330.
5. Matsumoto, H., Erickson, R.H., Gum, J.R., Yoshioka, M., Gum, E. & Kim, Y.S. (1990). Biosynthesis of alkaline phosphatase during differentiation of the human colon cancer cell line Caco-2. Gastroenterology 98, 1199-1207.
6. Levy, P., Robert, A. & Picard, J. (1988). Biosynthesis of glycosaminoglycans in the human colonic tumor cell line Caco-2: structural changes occurring with the morphological differentiation of the cells. Biology of the Cell 62, 255-264.
7. Levy, P., Cherqui, G., Robert, A., Wicek, D. & Picard, J. (1989). Changes in glycosaminoglycan sulfation and protein kinase C subcellular distribution during differentiation of the human colon tumor cell line Caco-2. Experientia 45, 588-591.
8. Hidalgo, I.J., Raub, T.J. & Borchardt, R.T. (1989). Characterization of the human colon carcinoma cell line (Caco-2) as a model system for intestinal epithelial permeability. Gastroenterology 96, 736-749.
9. Sambuy, Y., De Angelis, I., Ranaldi, G., Scarino, M.L., Stammati, A. & Zucco, F. (2005). The Caco-2 cell line as a model of the intestinal barrier: influence of cell and culture-related factors on Caco-2 cell functional characteristics. Cell Biology and Toxicology 21, 1-26.
10. Bjorge, S., Hamelehle, K.L., Roman, R., Rose, S.E., Turluck, D.A. & Wright, D.S. (1991). Evidence for glucuronide conjugation of p-nitrophenol in the Caco-2 cell model. Pharmacological Research 8, 1441-1443.
11. Baranczyk-Kuzma, A., Garren, J.A., Hidalgo, I.J. & Borchardt, R.T. (1991). Substrate specificity and some properties of phenol sulfotransferase from human intestinal Caco-2 cells. Life Sciences 49, 1197-1206.
12. Boulenc, X., Bourrie, M., Fahre, I., Roque, G., Joyeux, H., Berger, Y. & Fabre, G. (1992). Regulation of cytochrome P4501A1 gene expression in a human intestinal cell line, Caco-2. Journal of Pharmacology and Experimental Therapeutics 263, 1471-1478.
13. Peters, W.H.M. & Roelofs, H.M.J. (1992). Biochemical characterisation of resistance to mitoxantrone and adriamycin in Caco-2 human colon carcinoma cells: a possible role of glutathione-S-transferase. Cancer Research 52, 1886-1890.
14. Prueksaritanont, T., Gorham, L.M., Hochman, J.H., Tran, L.O. & Vyas, K.P. (1996). Comparative studies of drug-metabolising enzymes in dog, monkey and human small intestines and in Caco-2 cells. Drug Metaboli73m and Disposition 24, 634-642.
15. Lampen, A., Bader, A., Bestmann, T., Winkler, M., Witte, L. & Borlak, J.T. (1998). Catalytic activities, protein and mRNA expression of cytochrome P450 isoenzymes in intestinal cell lines. Xenobiotica 28, 429-441.
16. Augustijns, P., Annaert, P.P.H., Van den Mooter, G. & Kinget, R. (1998). Drug absorption studies of pro-drug esters using the Caco-2 model: evaluation of ester hydrolysis and transepithelial transport. International Journal of Pharmaceutics 166, 45-53.
17. De Angelis, I., Vincentini, O., Brambilla, G., Stammati, A. & Zucco, F. (1998). Characterization of furazolidone apical-related effects to human polarized intestinal cells. Toxicology and Applied Pharmacology 152, 119-127.
18. Chantret, I., Barbat, A., Dussaulx, E., Brattain, M.G. & Zweibaum, A. (1988). Epithelial polarity, villin expression and enterocytic differentiation of cultured human colon carcinoma cells: a survey of twenty cell lines. Cancer Research 48, 1936-1942.
19. Harris, D.S., Slot, J.W., Geuze, H.J. & James, D.E. (1992). Polarized distribution of glucose transporter isoforms in Caco-2 cells. Proceedings of the National Academy of Sciences USA 89, 7556-7560.
20. Engle, M.J., Goetz, G.S. & Alpers, D.H. (1998). Caco-2 cells express a combination of colonocyte and enterocyte phenotypes. Journal of Cell Physiology 174, 362-369.
21. Sambuy, Y., Ferruza, S., Ranaldi, G. & De Angelis, I. (2001). Intestinal cell culture models. Applications in toxicology and pharmacology. Cell Biology and Toxicology 17, 301-317.
22. Delie, F. & Kubas, W. (1997). A human colonic cell line sharing similarities with enterocytes as a model to examine oral absorption: Advantages and limitations of the Caco-2 model. Critical Review in Therapeutic Drug Carrier Systems 14, 221-286.
23. Artursson, P., Palm, K. & Luthman, K. (2001). Caco-2 monolayers in experimental and theoretical predictions of drug transport. Advanced Drug Delivery Reviews 46, 27-43.
24. ISS/ECVAM (2003). Final Report of Development and refinement of a Caco-2 cell in vitro model of intestinal barrier function. Contract number ISS/ECVAM 17299-2000-12. Website http://ecvam.jrc.it
25. Madara, J. (1998). Regulation of the movement of solutes across tight junctions. Annual Review of Physiology 60, 143-159.
26. Spring, K. (1998). Routes and mechanism of fluid transport by epithelia. Annual Review of Physiology 60, 105-119.
27. Sussman, N.L., Eliakim, R., Rubin, D., Perlmutter, D.H., Deschryver-Keckemeti, K. & Alpers, D.H. (1989). Intestinal alkaline phosphatase is secreted bidirectionally from villous enterocytes. American Journal of Physiology 275, G14-G23.
28. Chantret, I., Rodolosse, A., Barbat, A., Dussaulx, E., Brot-Laroche, E., Zweibaum, A. & Rousset, M. (1994). Differential expression of sucrase-isomaltase in clones isolated from early and late passages of the cell line Caco-2: evidence for glucose-dependent negative regulation. Journal of Cell Science 107, 213-225.
29. Beaulieu, J-F. & Quaroni, A. (1991). Clonal analysis of sucrase-isomaltase expression in the human colon adenocarcinoma Caco-2 cells. Biochemical Journal 280, 599-608.
30. 2+ transport in Caco-2 cell cultures. American Journal of Physiology 274, G389-396.
31. Morel, E., Demignote, S., Chateau, D., Chambaz, J., Rousset, M. & Delers, F. (2004). Lipid-dependent bidirectional traffic of apolipoprotin B in polarized enterocytes. Molecular Biology of the Cell 15, 132-141.
32. Lowry, O.H., Rosenbrough, N.J., Farr, A.L. & Randall, R.I. (1951). Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193, 265-275.
33. Bradford, M. (1976). A rapid sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein dye binding. Analytical Biochemistry 72, 248-254.
34. Balls, M., Blaauboer, B.J., Fentem, J.H., Bruner, L., Combes, R.D., Ekwall, B., Fielder, R.J., Guillouzo, A., Lewis, R.W., Lovell, D.P., Reinhardt, C.A., Repetto, G., Sladowski, D., Spielmann, H. & Zucco, F. (1995). Practical aspects of the validation of toxicity test procedures. ATLA 23, 129-147.
35. Artursson, P. (1990). Epithelial transport of drugs in cell culture. I: A model for studying the passive diffusion of drugs over intestinal absorptive (Caco-2) cells. Journal of Pharmaceutical Science 79, 476-482.
36. Tsukita, S. & Furuse, M. (1999). Occludin and claudins in tight-junction strands: leading or supporting players? Trends in Cell Biology 9, 268-273.
37. D'Atri, F. & Citi, S. (2002). Molecular complexity of vertebrate tight junctions. Molecular Membrane Biology 19, 103-112.