Copper treatment alters the permeability of tight junctions in cultured human intestinal Caco-2 cells

American Journal of Physiology - Gastrointestinal and Liver Physiology

Volumn 277, Issue 6 40-6, 1999, Pages

  Ferruzza, Simonetta ^a   Scarino, Maria Laura ^a   Rotilio, Giuseppe ^a,b   Ciriolo, Maria Rosa ^c   Santaroni, Paolo ^a   Muda, Andrea Onetti ^d   Sambuy, Yula ^a  

^a ISTITUTO NAZIONALE DELLA NUTRIZIONE   (Italy)

^b UNIVERSITY OF ROME TOR VERGATA   (Italy)

^c UNIVERSITY OF CHIETI   (Italy)

^d SAPIENZA UNIVERSITY OF ROME   (Italy)

Author keywords

Copper histidine; CuCl2; F actin organization; Junctional proteins; Microvilli; PH; Recovery; Uptake

Indexed keywords

COPPER; F ACTIN; OCCLUDIN; UVOMORULIN;

ARTICLE; CELL MEMBRANE PERMEABILITY; CELL ULTRASTRUCTURE; CONTROLLED STUDY; CYTOSKELETON; HUMAN; HUMAN CELL; INTESTINE ABSORPTION; INTESTINE BRUSH BORDER; INTESTINE CELL; PRIORITY JOURNAL; PROTEIN LOCALIZATION; TIGHT JUNCTION;

EID: 0033400532     PISSN: 01931857     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajpgi.1999.277.6.g1138     Document Type: Article

References (39)

1. Alvarez-Hernandez, X., M. Smith, and J. Glass. The effect of apotransferrin on iron release from Caco-2 cells, an intestinal epithelial cell line. Blood 91: 3974-3979, 1998.
2. Brandtzaeg, P. Mechanisms of gastrointestinal reactions to food. Environ. Toxicol. Pharmacol. 4: 9-24, 1997.
3. Briske-Anderson, M., J. Finlay, and S. Newman. The influence of culture time and passage number on the morphological and physiological development of Caco-2 cells. Proc. Soc. Exp. Biol. Med. 214: 248-257, 1997.
4. Da Costa Ferreira, A., M. Ciriolo, L. Marcocci, and G. Rotilio. Copper(I) transfer into metallothionein mediated by glutathione. Biochem. J. 292: 673-676, 1993.
5. Dameron, C., and M. Harrison. Mechanisms for protection against copper toxicity. Am. J. Clin. Nutr. 67, Suppl.: 1091S-1097S, 1998.
6. De Angelis, I., O. Vincentini, G. Brambilla, A. Stammati, and F. Zucco. Characterization of furazolidone apical-related effects to human polarized intestinal cells. Toxicol. Appl. Pharmacol. 152: 119-127, 1998.
7. Denker, B., and S. Nigam. Molecular structure and assembly of the tight junction. Am. J. Physiol. 274 (Renal Physiol. 43): F1-F9, 1998.
8. Fagotti, A., I. Di Rosa, F. Simoncelli, R. Pipe, F. Panara, and R. Pascolini. The effects of copper on actin and fibronectin organization in Mytilus galloprovincialis in haematocytes. Dev. Comp. Immunol. 20: 383-391, 1996.
9. Ferruzza, S., G. Ranaldi, M. Di Girolamo, and Y. Sambuy. The transport of lysine across monolayers of human cultured intestinal cells (Caco-2) depends on Na-dependent and Naindependent mechanisms on different plasma membrane domains. J. Nutr. 125: 2577-2585, 1995.
10. Ferruzza, S., Y. Sambuy, G. Rotilio, M. Ciriolo, and M. Scarino. The effect of copper on tight junctional permeability in a human intestinal cell line (Caco-2). In: Copper Transport and its Disorders: Molecular and Cellular Aspects, edited by A. Leone and J. Mercer. New York: Plenum, 1999, p. 215-222.
11. Fuentealba, I., and S. Haywood. Cellular mechanisms of toxicity and tolerance in the copper-loaded rat. I. Ultrastructural changes in the liver. Liver 8: 372-380, 1988.
12. Gunshin, H., B. Mackenzie, U. Berger, Y. Gunshin, M. Romero, W. Boron, S. Nussberger, J. Gollan, and M. Hediger. Cloning and characterization of a mammalian proton-coupled metal-ion transporter. Nature 338: 482-488, 1997.
13. Han, O., M. Failla, A. Hill, E. Morris, and J. Smith. Reduction of Fe(III) is required for uptake of non-heme iron by Caco-2 cells. J. Nutr. 125: 1291-1299, 1995.
14. Hecht, G., C. Pothoulakis, J. T. La Mont, and J. L. Madara. Clostridium difficile toxin A perturbs cytoskeletal structure and tight junction permeability of cultured human intestinal epithelial monolayers. J. Clin. Invest. 82: 1516-1524, 1988.
15. Kim, E., and E. Reisler. Intermolecular coupling between loop 38-52 and the C-terminus in actin filaments. Biophys. J. 71: 1914-1919, 1996.
16. Lange, J., K. Schlieps, K. Lange, and E. Knoll-Kohler. Activation of calcium signaling in isolated rat hepatocytes is accompanied by shape changes of microvilli. Exp. Cell Res. 234: 486-497, 1997.
17. Leibach, F., and V. Ganapathy. Peptide transporters in the intestine and the kidney. Annu. Rev. Nutr. 16: 99-119, 1997.
18. Linder, M. Copper. In: Present Knowledge in Nutrition (7th ed.), edited by E. Ziegler and L. Filer. Washington, DC: ILSI, 1996, p. 307-319.
19. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265-275, 1951.
20. Ma, T., D. Hollander, L. Tran, T. Nguyen, N. Hoa, and D. Bhalla. Cytoskeletal regulation of Caco-2 intestinal monolayer paracellular permeability. J. Cell. Physiol. 164: 533-545, 1995.
21. Ma, T., D. Nguyen, V. Bui, H. Nguyen, and N. Hoa. Ethanol modulation of intestinal epithelial tight junction barrier. Am. J. Physiol. 276 (Gastrointest. Liver Physiol. 39): G965-G974, 1999.
22. Madara, J. Regulation of the movement of solutes across tight junctions. Annu. Rev. Physiol. 60: 143-159, 1998.
23. Matter, K., and M. Balda. Occludin and the functions of tight junctions. Int. Rev. Cytol. 186: 117-146, 1999.
24. McArdle, H., S. Gross, D. Danks, and A. Wedd. Role of albumin's copper binding site in copper uptake by mouse hepatocytes. Am. J. Physiol. 258 (Gastrointest. Liver Physiol. 21): G988-G991, 1990.
25. McArdle, H., S. M. Gross, and D. Danks. Uptake of copper by mouse hepatocytes. J. Cell. Physiol. 136: 373-378, 1988.
26. Mitic, L., and J. Anderson. Molecular architecture of tight junctions. Annu. Rev. Physiol. 60: 121-142, 1998.
27. Nurko, S., K. Sogabe, J. Davis, N. Roeser, M. Defrain, A. Chien, D. Hinshaw, B. Athey, W. Meixner, M. Venkatachalam, and J. Weinberg. Contribution of actin cytoskeleton alterations to ATP depletion and calcium-induced proximal tubule cell injury. Am. J. Physiol. 270 (Renal Fluid Electrolyte Physiol. 39): F39-F52, 1996.
28. Olivares, M., and R. Uauy. Limits of metabolic tolerance to copper and biological basis for present recommendations and regulations. Am. J. Clin. Nutr. 63, Suppl.: 846S-852S, 1996.
29. Pinto, M., S. Robine-Leon, M. Appay, M. Kedinger, E. Triadou, E. Dussaulx, B. Lacroix, P. Simon-Assman, K. Haffen, J. Fogh, and A. Zweibaum. Enterocyte-like differentiation and polarization of the human colon carcinoma cell line Caco-2 in culture. Biol. Cell 47: 323-330, 1983.
30. Ranaldi, G., K. Islam, and Y. Sambuy. D-Cycloserine uses an active transport mechanism in the human intestinal cell line Caco 2. Antimicrob. Agents Chemother. 38: 1239-1245, 1994.
31. Reeves, P., M. Briske-Anderson, and L. Johnson. Physiological concentrations of zinc affect the kinetics of copper uptake and transport in the human intestinal cell model. Caco-2. J. Nutr. 128: 1794-1801, 1998.
32. Rossi, A., R. Poverini, G. Di Lullo, A. Modesti, A. Modica, and M. Scarino. Heavy metal toxicity following apical and basolateral exposure in the human intestinal cell line Caco-2. Toxicol. In Vitro 10: 27-36, 1996.
33. Sakai, M., T. Imai, H. Ohtake, H. Azuma, and M. Otagiri. Effect of absorption enhancers on the transport of model compounds in Caco-2 cell monolayers: assessment by confocal laser scanning microscopy. J. Pharm. Res. 86: 779-785, 1997.
34. Sandstead, H. Requirements and toxicity of essential trace elements illustrated by zinc and copper. Am. J. Clin. Nutr. 61 (Suppl.): 621S-624S, 1995.
35. Scarino, M. L., R. Poverini, G. Di Lullo, and G. Bises. Metallothionein gene expression in the intestinal cell; modulation of mRNA and protein synthesis by copper and zinc. Biochem. Soc. Trans. 19: 238S, 1991.
36. Tapia, V., M. Arredondo, and M. Nunez. Regulation of Fe absorption by cultured intestinal epithelia (Caco-2) cell monolayers with varied Fe status. Am. J. Physiol. 271 (Gastrointest. Liver Physiol. 34): G443-G447, 1996.
37. Thwaites, D., G. McEwan, and N. Simmons. The role of the proton electrochemical gradient in the transepithelial absorption of amino acids by human intestinal Caco-2 cell monolayers. J. Membr. Biol. 145: 245-256, 1995.
38. Wang, Z., and D. Templeton. Cellular factors mediate cadmium-dependent actin depolymerization. Toxicol. Appl. Pharmacol. 139: 115-121, 1996.
39. Wapnir, R. Copper absorption and bioavailability. Am. J. Clin. Nutr. 67, Suppl.: 1054S-1060S, 1998.