Overexpression of manganese superoxide dismutase does not increase clonogenic cell survival despite effect on apoptosis in irradiated lymphoblastoid cells

Radiation Research

Volumn 176, Issue 6, 2011, Pages 725-731

  Veldwijk, Marlon R ^a,b   Trah, Julian ^a   Wang, Meng ^a   Maier, Patrick ^a   Fruehauf, Stefan ^c   Zeller, W Jens ^b   Herskind, Carsten ^a   Wenz, Frederik ^a  

^a UNIVERSITY OF HEIDELBERG   (Germany)

^b GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

^c Paracelsus Klinik   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

COPPER ZINC SUPEROXIDE DISMUTASE; LENTIVIRUS VECTOR; MANGANESE SUPEROXIDE DISMUTASE;

APOPTOSIS; ARTICLE; CELL SURVIVAL; CONTROLLED STUDY; ENZYME ACTIVITY; HUMAN; HUMAN CELL; IRRADIATION; LYMPHOBLASTOID CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; RADIATION DOSE;

APOPTOSIS; CELL LINE, TUMOR; CELL SURVIVAL; CLONING, MOLECULAR; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENETIC VECTORS; HEK293 CELLS; HUMANS; LENTIVIRUS; LYMPHOCYTES; SUPEROXIDE DISMUTASE; SUPEROXIDES;

EID: 82955168395     PISSN: 00337587     EISSN: 19385404     Source Type: Journal    
DOI: 10.1667/RR2651.1     Document Type: Article

References (42)

1. Mann T, Keilin D. Proc Roy Soc Ser B (London) 1939; 126:303-15.
2. McCord JM, Fridovich I. Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein). J Biol Chem 1969; 244:6049-55.
3. Keele BB Jr, McCord JM, Fridovich I. Superoxide dismutase from Escherichia coli B. A new manganese-containing enzyme. J Biol Chem 1970; 245:6176-81.
4. Epperly MW, Bray JA, Krager S, Berry LM, Gooding W, Engelhardt JF, et al. Intratracheal injection of adenovirus containing the human MnSOD transgene protects athymic nude mice from irradiation-induced organizing alveolitis. Int J Radiat Oncol Biol Phys 1999; 43:169-81. (Pubitemid 29065842)
5. Epperly MW, Epperly LD, Niu Y, Wang H, Zhang X, Franicola D, et al. Overexpression of the MnSOD transgene product protects cryopreserved bone marrow hematopoietic progenitor cells from ionizing radiation. Radiat Res 2007; 168:560-6. (Pubitemid 351295119)
6. Veldwijk MR, Herskind C, Sellner L, Radujkovic A, Laufs S, Fruehauf S, et al. Normal-tissue radioprotection by overexpression of the copper-zinc and manganese superoxide dismutase genes. Strahlenther Onkol 2009; 185:517-23.
7. Vozenin-Brotons MC, Sivan V, Gault N, Renard C, Geffrotin C, Delanian S, et al. Antifibrotic action of Cu/Zn SOD is mediated by TGF-beta1 repression and phenotypic reversion of myofibroblasts. Free Radic Biol Med 2001; 30:30-42. (Pubitemid 32008086)
8. Epperly MW, Sikora CA, DeFilippi SJ, Gretton JA, Zhan Q, Kufe DW, et al. Manganese superoxide dismutase (SOD2) inhibits radiation-induced apoptosis by stabilization of the mitochondrial membrane. Radiat Res 2002; 157:568-77. (Pubitemid 34492564)
9. Noshita N, Sugawara T, Fujimura M, Morita-Fujimura Y, Chan PH. Manganese superoxide dismutase affects cytochrome c release and caspase-9 activation after transient focal cerebral ischemia in mice. J Cereb Blood Flow Metab 2001; 21:557-67. (Pubitemid 32422876)
10. Drane P, Bravard A, Bouvard V, May E. Reciprocal downregulation of p53 and SOD2 gene expression-implication in p53 mediated apoptosis. Oncogene 2001; 20:430-9. (Pubitemid 32158223)
11. Akashi M, Hachiya M, Paquette RL, Osawa Y, Shimizu S, Suzuki G. Irradiation increases manganese superoxide dismutase mRNA levels in human fibroblasts. Possible mechanisms for its accumulation. J Biol Chem 1995; 270:15864-9.
12. Hardmeier R, Hoeger H, Fang-Kircher S, Khoschsorur A, Lubec G. Transcription and activity of antioxidant enzymes after ionizing irradiation in radiation-resistant and radiation-sensitive mice. Proc Natl Acad Sci USA 1997; 94:7572-6. (Pubitemid 27345357)
13. Riley PA. Free radicals in biology: oxidative stress and the effects of ionizing radiation. Int J Radiat Biol 1994; 65:27-33. (Pubitemid 24063526)
14. Sun J, Chen Y, Li M, Ge Z. Role of antioxidant enzymes on ionizing radiation resistance. Free Radic Biol Med 1998; 24:586-93. (Pubitemid 28150477)
15. Levy JA, Virolainen M, Defendi V. Human lymphoblastoid lines from lymph node and spleen. Cancer 1968; 22:517-24.
16. Liber HL, Thilly WG. Mutation assay at the thymidine kinase locus in diploid human lymphoblasts. Mutat Res 1982;94:467-85. (Pubitemid 12074552)
17. Yu Y, Little JB. p53 is involved in but not required for ionizing radiation-induced caspase-3 activation and apoptosis in human lymphoblast cell lines. Cancer Res 1998; 58:4277-81. (Pubitemid 28450025)
18. Kimmel RR, Agnani S, Yang Y, Jordan R, Schwartz JL. DNA copy-number instability in low-dose gamma-irradiated TK6 lymphoblastoid clones. Radiat Res 2008; 169:259-69. (Pubitemid 351521761)
19. Maier P, Fleckenstein K, Li L, Laufs S, Zeller WJ, Baum C, et al. Overexpression of MDR1 using a retroviral vector differentially regulates genes involved in detoxification and apoptosis and confers radioprotection. Radiat Res 2006; 166:463-73. (Pubitemid 44353429)
20. Schafer J, Bachtler J, Engling A, Little JB, Weber KJ, Wenz F. Suppression of apoptosis and clonogenic survival in irradiated human lymphoblasts with different TP53 status. Radiat Res 2002; 158:699-706. (Pubitemid 35429964)
21. Wenz F, Azzam EI, Little JB. The response of proliferating cell nuclear antigen to ionizing radiation in human lymphoblastoid cell lines is dependent on p53. Radiat Res 1998; 149:32-40. (Pubitemid 28022893)
22. Rasheed S, Nelson-Rees WA, Toth EM, Arnstein P, Gardner MB. Characterization of a newly derived human sarcoma cell line (HT-1080). Cancer 1974; 33:1027-33.
23. Veldwijk MR, Herskind C, Laufs S, Zeller WJ, Fruehauf S, Wenz F. Recombinant adeno-associated virus 2-mediated transfer of the human superoxide-dismutase gene does not confer radioresistance on HeLa cervical carcinoma cells. Radiother Oncol 2004; 72:341-50. (Pubitemid 39265389)
24. Demaison C, Parsley K, Brouns G, Scherr M, Battmer K, Kinnon C, et al. High-level transduction and gene expression in hematopoietic repopulating cells using a human immunodeficiency [correction of imunodeficiency] virus type 1-based lentiviral vector containing an internal spleen focus forming virus promoter. Hum Gene Ther 2002; 13:803-13. (Pubitemid 34493974)
25. Maier P, Herskind C, Fleckenstein K, Spier I, Laufs S, Zeller WJ, et al. MDR1 gene transfer using a lentiviral SIN vector confers radioprotection to human CD34\+ hematopoietic progenitor cells. Radiat Res 2008; 169:301-10. (Pubitemid 351521765)
26. Scherr M, Battmer K, Blomer U, Ganser A, Grez M. Quantitative determination of lentiviral vector particle numbers by real-time PCR. Biotechniques 2001; 31:520-4.
27. Nicoletti I, Migliorati G, Pagliacci MC, Grignani F, Riccardi C. A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. J Immunol Methods 1991; 139:271-9.
28. Peskin AV, Winterbourn CC. A microtiter plate assay for superoxide dismutase using a water-soluble tetrazolium salt (WST-1). Clin Chim Acta 2000; 293:157-66. (Pubitemid 30103563)
29. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983; 65:55-63. (Pubitemid 14203433)
30. Veldwijk MR, Berlinghoff S, Laufs S, Hengge UR, Zeller WJ, Wenz F, et al. Suicide gene therapy of sarcoma cell lines using recombinant adeno-associated virus 2 vectors. Cancer Gene Ther 2004; 11:577-84. (Pubitemid 39079496)
31. Furth EE, Thilly WG, Penman BW, Liber HL, Rand WM. Quantitative assay for mutation in diploid human lymphoblasts using microtiter plates. Anal Biochem 1981; 110:1-8. (Pubitemid 11157545)
32. Maier P, Herskind C, Barzan D, Zeller WJ, Wenz F. SNAI2 as a novel radioprotector of normal tissue by gene transfer using a lentiviral bicistronic SIN vector. Radiat Res 2010; 173:612-9.
33. Bus JS, Gibson JE. Paraquat: model for oxidant-initiated toxicity. Environ Health Perspect 1984; 55:37-46. (Pubitemid 14094796)
34. Epperly MW, Gretton JE, Sikora CA, Jefferson M, Bernarding M, Nie S, et al. Mitochondrial localization of superoxide dismutase is required for decreasing radiation-induced cellular damage. Radiat Res 2003; 160:568-78. (Pubitemid 38055205)
35. Epperly MW, Carpenter M, Agarwal A, Mitra P, Nie S, Greenberger JS. Intraoral manganese superoxide dismutaseplasmid/liposome (MnSOD-PL) radioprotective gene therapy decreases ionizing irradiation-induced murine mucosal cell cycling and apoptosis. In Vivo 2004; 18:401-10. (Pubitemid 39199983)
36. Epperly MW, Defilippi S, Sikora C, Gretton J, Kalend A, Greenberger JS. Intratracheal injection of manganese superoxide dismutase (MnSOD) plasmid/liposomes protects normal lung but not orthotopic tumors from irradiation. Gene Ther 2000; 7:1011-8. (Pubitemid 30398349)
37. Guo H, Epperly MW, Bernarding M, Nie S, Gretton J, Jefferson M, et al. Manganese superoxide dismutase-plasmid/liposome (MnSOD-PL) intratracheal gene therapy reduction of irradiationinduced inflammatory cytokines does not protect orthotopic Lewis lung carcinomas. In Vivo 2003; 17:13-21. (Pubitemid 36382960)
38. Zhong W, Oberley LW, Oberley TD, Yan T, Domann FE, St Clair DK. Inhibition of cell growth and sensitization to oxidative damage by overexpression of manganese superoxide dismutase in rat glioma cells. Cell Growth Differ 1996; 7:1175-86. (Pubitemid 26360698)
39. Aykin-Burns N, Ahmad IM, Zhu Y, Oberley L, Spitz DR. Increased levels of superoxide and hydrogen peroxide mediate the differential susceptibility of cancer cells vs. normal cells to glucose deprivation. Biochem J 2009; 418:29-37.
40. Duan H, Zhang HJ, Yang JQ, Oberley LW, Futscher BW, Domann FE. MnSOD up-regulates maspin tumor suppressor gene expression in human breast and prostate cancer cells. Antioxid Redox Signal 2003;5(5):677-88. (Pubitemid 37315157)
41. Urano M, Kuroda M, Reynolds R, Oberley TD, St Clair DK. Expression of manganese superoxide dismutase reduces tumor control radiation dose: gene-radiotherapy. Cancer Res 1995; 55:2490-3.
42. Amstad PA, Liu H, Ichimiya M, Berezesky IK, Trump BF. Manganese superoxide dismutase expression inhibits soft agar growth in JB6 clone41 mouse epidermal cells. Carcinogenesis 1997; 18:479-84. (Pubitemid 28132483)