Quantitative determination of lentiviral vector particle numbers by real-time PCR

BioTechniques

Volumn 31, Issue 3, 2001, Pages 520-526

  Scherr, M ^a   Battmer, K ^a   Blomer U ^a   Ganser, A ^a   Grez, M ^a  

^a HANNOVER MEDICAL SCHOOL   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; VIRUSES;

INFECTIOUS PARTICLES;

BIOTECHNOLOGY;

VIRUS VECTOR;

ARTICLE; LENTIVIRINAE; NONHUMAN; POLYMERASE CHAIN REACTION; TARGET CELL; VIRUS CELL INTERACTION; VIRUS PARTICLE;

EID: 0034857455     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/01313st05     Document Type: Article

References (13)

1. Novel approach to quantitative polymerase chain reaction using real-time detection: Application to the detection of gene amplification in breast cancer
2. Highly efficient and sustained gene transfer in adult neurons with a lentivirus vector
3. High-efficiency transformation of mammalian cells by plasmid-DNA
4. + cells via human immunodeficiency virus-based gene transfer vectors
5. Real-time quantitative PCR
6. 4+ cells that mediate long-term engraftment of NOD/SCID mice by HIV vectors
7. A rapid screening procedure for the identification of high-titer retrovirus packaging clones
8. Efficient transfer, integration, and sustained long-term expression of the transgene in adult rat brains injected with a lentiviral vector
9. Rapid titer determination using quantitative real-time PCR
10. One step screening of retroviral producer clones by real time quantitative PCR
11. Partial reverse transcripts in virions from human immunodeficiency and murine leukemia viruses
12. Self-inactivating lentivirus vector for safe and efficient in vivo gene delivery
13. Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo