High-level production of a kringle domain variant by high-cell-density cultivation of Escherichia coli

Applied Microbiology and Biotechnology

Volumn 92, Issue 2, 2011, Pages 327-336

  Jang, Seung Hoon ^a   Lee, Chang Han ^b   Kim, Yong Sung ^b   Jeong, Ki Jun ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

^b Ajou University   (South Korea)

Author keywords

Escherichia coli; Fusion tag; High cell density culture; Kringle domain

Indexed keywords

BINDING PROTEINS; CELL DENSITY; DISULFIDE BONDS; E. COLI; EXPRESSION SYSTEM; FEEDING SOLUTIONS; FUSION TAG; GOOD YIELD; HIGH ACTIVITY; HIGH CELL DENSITY; HIGH-CELL-DENSITY CULTIVATIONS; HIGH-LEVEL PRODUCTION; KRINGLE DOMAIN; PRODUCTION YIELD; PROTEIN EXPRESSIONS; SHAKE FLASK CULTIVATIONS; SOLUBLE PROTEINS;

CELL CULTURE; CELLS; COVALENT BONDS; ESCHERICHIA COLI; PROTEINS;

SCAFFOLDS (BIOLOGY);

ANTINEOPLASTIC AGENT; DISULFIDE; KD 548; LAC REPRESSOR; POLYHISTIDINE TAG; PROTEIN; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ANTIBODY; BIOREACTOR; CANCER; DISEASE TREATMENT; FECAL COLIFORM; GENE EXPRESSION; GENETIC ENGINEERING; INDUSTRIAL PRODUCTION;

ANIMAL CELL; ARTICLE; BACTERIAL STRAIN; BACTERIUM CULTURE; BIOREACTOR; CELL DENSITY; CONTROLLED STUDY; CYTOPLASM; DISULFIDE BOND; DRUG DESIGN; ESCHERICHIA COLI; FEEDING; GENE EXPRESSION SYSTEM; KRINGLE DOMAIN; LARGE SCALE PRODUCTION; NONHUMAN; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN FOLDING;

BLOOD PROTEINS; ESCHERICHIA COLI; GENE EXPRESSION; HUMANS; KINETICS; KRINGLES; RECOMBINANT PROTEINS;

ESCHERICHIA COLI; PICHIA;

EID: 82455208943     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-011-3423-z     Document Type: Article

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