Multivalent avimer proteins evolved by exon shuffling of a family of human receptor domains

Nature Biotechnology

Volumn 23, Issue 12, 2005, Pages 1556-1561

  Silverman, Joshua ^a   Lu, Qiang ^a   Bakker, Alice ^a   To, Wayne ^a   Duguay, Amy ^a   Alba, Ben M ^a   Smith, Richard ^a   Rivas, Alberto ^a   Li, Peng ^a   Le, Hon ^a   Whitehorn, Erik ^a   Moore, Kevin W ^a   Swimmer, Candace ^a   Perlroth, Victor ^a   Vogt, Martin ^a   Kolkman, Joost ^a   Stemmer, Willem Pim C ^a  

^a Avidia Research Institute   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODIES; CELL CULTURE; ENZYME INHIBITION; ESCHERICHIA COLI; IMMUNOLOGY; MATHEMATICAL MODELS;

AVIMERS; EXON SHUFFLING;

PROTEINS;

AVIMER PROTEIN C 2; AVIMER PROTEIN C 2810; AVIMER PROTEIN C 326; AVIMER PROTEIN C 426; AVIMER PROTEIN C 65; BINDING PROTEIN; CD28 ANTIGEN; CD40 LIGAND; IMMUNOGLOBULIN; IMMUNOLOGIC AGENT; INTERLEUKIN 6; PROTEIN DERIVATIVE; PROTEINASE; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; DRUG BINDING; DRUG HALF LIFE; ESCHERICHIA COLI; EXON; FEMALE; HUMAN; HUMAN CELL; IC 50; MOUSE; NONHUMAN; PHAGE DISPLAY; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; THERMOSTABILITY;

ANIMALS; DNA SHUFFLING; EVOLUTION, MOLECULAR; EXONS; FEMALE; HUMANS; MICE; PROTEIN ENGINEERING; PROTEIN STRUCTURE, TERTIARY; RECEPTORS, CELL SURFACE;

ANIMALIA; ESCHERICHIA COLI;

EID: 28644436100     PISSN: 10870156     EISSN: 15461696     Source Type: Journal    
DOI: 10.1038/nbt1166     Document Type: Article

References (18)

1. Huang, W., Dolmer, K. & Gettins, P.G. NMR solution structure of complement-like repeat CR8 from the low density lipoprotein receptor-related protein. J. Biol. Chem. 274, 14130-14136 (1999).
2. North, C.L. & Blacklow, S.C. Structural independence of ligand-binding modules five and six of the LDL receptor. Biochemistry 38, 3926-3935 (1999).
3. Gliemann, J. Receptors of the low density lipoprotein (LDL) receptor family in man. Multiple functions of the large family members via interaction with complex ligands. Biol. Chem. 379, 951-964 (1998).
4. Krieger, M. & Herz, J. Structures and functions of multiligand lipoprotein receptors: macrophage scavenger receptors and LDL receptor-related protein (LRP). Annu. Rev. Biochem. 63, 601-637 (1994).
5. Rettenberger, P.M. et al. Ligand binding properties of the very low density lipoprotein receptor. Absence of the third complement-type repeat encoded by exon 4 is associated with reduced binding of Mr 40,000 receptor-associated protein. J. Biol. Chem. 274, 8973-8980 (1999).
6. Mammen, M., Choi, S.K. & Whitesides, G.M. Polyvalent interactions in biological systems: implications for design and use of multivalent ligands and inhibitors. Angew. Chem. Int. Ed. 37, 2755-2794 (1998).
7. Koduri, V. & Blacklow, S.C. Folding determinants of LDL receptor type A modules. Biochemistry 40, 12801-12807 (2001).
8. Physicians Desk Reference, edn. 59 (Medical Economics, Montvale, N.J., 2005).
9. Suffredini, A.F., Fantuzzi, G., Badolato, R., Oppenheim, J.J. & O'Grady, N.P. New insights into the biology of the acute phase response. J. Clin. Immunol. 19, 203-214 (1999).
10. Chang, C.C. et al. Evolution of a cytokine using DNA family shuffling. Nat. Biotechnol. 17, 793-797 (1999).
11. Crameri, A., Raillard, S.A., Bermudez, E. & Stemmer, W.P. DNA shuffling of a family of genes from diverse species accelerates directed evolution. Nature 391, 288-291 (1998).
12. Patten, P.A. & Schellekens, H. The immunogenicity of biopharmaceuticals. Lessons learned and consequences for protein drug development. Dev. Biol. (Basel) 112, 81-97 (2003).
13. Binz, H.K. & Pluckthun, A. Engineered proteins as specific binding reagents. Curr. Opin. Biotechnol. 16, 459-469 (2005).
14. Stemmer, W.P., Crameri, A., Ha, K.D., Brennan, T.M. & Heyneker, H.L. Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides. Gene 164, 49-53 (1995).
15. Stemmer, W.P.C. in The Encyclopedia of Molecular Biology, 447-457, (VCH Publishers, New York, 1996).
16. Szybalski, W., Kim, S.C., Hasan, N. & Podhajska, A.J. Class-IIS restriction enzymes - a review. Gene 100, 13-26 (1991).
17. Coulie, P.G., Stevens, M. & Van Snick, J. High- and low-affinity receptors for murine interleukin 6. Distinct distribution on B and T cells. Eur. J. Immunol. 19, 2107-2114 (1989).
18. Hammacher, A. et al. Structure-function analysis of human IL-6: identification of two distinct regions that are important for receptor binding. Protein Sci. 3, 2280-2293 (1994).