Efficient expression and purification of human aglycosylated Fcγ receptors in Escherichia coli

Biotechnology and Bioengineering

Volumn 107, Issue 1, 2010, Pages 21-30

  Jung, Sang Taek ^a   Kang, Tae Hyun ^a   Georgiou, George ^a,b  

^a The University of Texas at Austin   (United States)

^b UNIVERSITY OF TEXAS AT AUSTIN   (United States)

Author keywords

Bacterial expression; Fc gamma receptor (Fc R); Glycosylation; IgG binding

Indexed keywords

BACTERIAL EXPRESSION; BINDING SELECTIVITY; CELLULAR RESPONSE; CROSSLINKED; CYTOKINES; CYTOTOXIC; E. COLI; EXTRACELLULAR DOMAINS; GLYCOSYLATED; HEMATOPOIETIC LINEAGE; HIGH YIELD; IGG BINDING; IMMUNE COMPLEX; INNATE IMMUNE RESPONSE; MAMMALIAN CELLS; N-TERMINALS; RS TRIGGER; SECONDARY STRUCTURE PREDICTION; SIGNALING CASCADES; TRANSMEMBRANES;

ANTIGENS; BACTERIOLOGY; COMPLEXATION; ESCHERICHIA COLI; ESTERIFICATION; GENE EXPRESSION; GLYCOSYLATION; MAMMALS; OPTIMIZATION; PURIFICATION;

ANTIBODIES;

FC RECEPTOR; IMMUNOGLOBULIN G1; IMMUNOGLOBULIN G2; IMMUNOGLOBULIN G3; IMMUNOGLOBULIN G4; MESSENGER RNA;

ARTICLE; ESCHERICHIA COLI; HUMAN; NONHUMAN; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN SECONDARY STRUCTURE; RECEPTOR BINDING;

ESCHERICHIA COLI; GENETIC ENHANCEMENT; GLYCOSYLATION; HUMANS; PROTEIN ENGINEERING; RECEPTORS, IGG; RECOMBINANT PROTEINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MAMMALIA;

EID: 77956260081     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22785     Document Type: Article

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