Preparation of an activated rhodopsin/transducin complex using a constitutively active mutant of rhodopsin

Biochemistry

Volumn 50, Issue 47, 2011, Pages 10399-10407

  Xie, Guifu ^a   D'Antona, Aaron M ^a   Edwards, Patricia C ^b   Fransen, Maikel ^b   Standfuss, Jorg ^c   Schertler, Gebhard F X ^c   Oprian, Daniel D ^a  

^a BRANDEIS UNIVERSITY   (United States)

^b MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

^c PAUL SCHERRER INSTITUTE   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVATED COMPLEX; BICELLES; CONSTITUTIVELY ACTIVES; COVALENTLY BOUND; DETERGENT SOLUTION; GUANINE NUCLEOTIDES; IMMUNOAFFINITY CHROMATOGRAPHY; IMMUNOAFFINITY COLUMNS; MOLAR RATIO; SUCROSE DENSITY GRADIENT CENTRIFUGATION; TRANSDUCIN;

AFFINITY CHROMATOGRAPHY; ANTIGEN-ANTIBODY REACTIONS; CENTRIFUGATION; CHROMOPHORES; COLUMN CHROMATOGRAPHY; GEL PERMEATION CHROMATOGRAPHY; NUCLEOTIDES; STOICHIOMETRY; SUGAR (SUCROSE);

SIZE EXCLUSION CHROMATOGRAPHY;

DETERGENT; GUANINE; MUTANT PROTEIN; RHODOPSIN; SEPHAROSE; TRANSDUCIN;

ARTICLE; CHROMATOPHORE; COMPLEX FORMATION; COVALENT BOND; COW; DENSITOMETRY; ELUTION; GEL PERMEATION CHROMATOGRAPHY; HUMAN; HUMAN CELL; IMMUNOAFFINITY CHROMATOGRAPHY; NONHUMAN; NUCLEOTIDE BINDING SITE; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; REPRODUCIBILITY; RETINA; STOICHIOMETRY; SUCROSE DENSITY GRADIENT CENTRIFUGATION; THERMOSTABILITY; WILD TYPE;

ANALYTIC SAMPLE PREPARATION METHODS; ANIMALS; CATTLE; CELL LINE; ENZYME ACTIVATION; HUMANS; MUTATION; NUCLEOTIDES; PROTEIN BINDING; RETINA; RHODOPSIN; TRANSDUCIN;

BOVINAE;

EID: 81855226606     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi201126r     Document Type: Article

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