High density micromass cultures of a human chondrocyte cell line: A reliable assay system to reveal the modulatory functions of pharmacological agents

Biochemical Pharmacology

Volumn 82, Issue 12, 2011, Pages 1919-1929

  Greco, K V ^c   Iqbal, A J ^c   Rattazzi, L ^c   Nalesso, G ^c   Moradi Bidhendi, N ^c   Moore, A R ^a   Goldring, M B ^b   Dell'Accio, F ^c   Perretti, M ^c  

^a UCB PHARMA   (Belgium)

^b HOSPITAL FOR SPECIAL SURGERY   (United States)

^c QUEEN MARY UNIVERSITY OF LONDON   (United Kingdom)

Author keywords

Anti inflammatory drugs; IL 1 ; Micromasses; Naproxen; Prednisolone; TGF 1

Indexed keywords

AGGRECAN; AGGRECANASE 2; BONE MORPHOGENETIC PROTEIN 2; COLLAGEN TYPE 1; COLLAGENASE 3; DISINTEGRIN; INTERLEUKIN 1BETA; INTERSTITIAL COLLAGENASE; NAPROXEN; PREDNISOLONE; TRANSCRIPTION FACTOR SOX9; TRANSFORMING GROWTH FACTOR BETA1;

ARTICLE; CARTILAGE CELL; CELL ASSAY; CELL CULTURE; CELL DENSITY; CELL PROLIFERATION; CHONDROSARCOMA; CONTROLLED STUDY; DOSE RESPONSE; DRUG MECHANISM; EVOKED RESPONSE; EXPRESSION VECTOR; GENE OVEREXPRESSION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IMMUNOMODULATION; OSTEOARTHRITIS; PHARMACODYNAMICS; PRIORITY JOURNAL; PROTEIN ANALYSIS; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SCREENING; STANDARDIZATION; WESTERN BLOTTING;

ALCIAN BLUE; ANTI-INFLAMMATORY AGENTS; CARTILAGE, ARTICULAR; CELL LINE; CELL PROLIFERATION; CHONDROCYTES; GENE EXPRESSION REGULATION; HUMANS; INTERLEUKIN-1BETA; NAPROXEN; PREDNISOLONE;

EID: 80555150580     PISSN: 00062952     EISSN: 18732968     Source Type: Journal    
DOI: 10.1016/j.bcp.2011.09.009     Document Type: Article

References (69)

1. R.C. Lawrence, D.T. Felson, C.G. Helmick, L.M. Arnold, H. Choi, and R.A. Deyo Estimates of the prevalence of arthritis and other rheumatic conditions in the United States. Part II Arthritis Rheum 58 1 2008 26 35
2. A.R. Poole, M. Kobayashi, T. Yasuda, S. Laverty, F. Mwale, and T. Kojima Type II collagen degradation and its regulation in articular cartilage in osteoarthritis Ann Rheum Dis 61 2 2002 78 81
3. M.B. Goldring The role of the chondrocyte in osteoarthritis Arthritis Rheum 43 9 2000 1916 1926
4. M. Otero, and M.B. Goldring Cells of synovium in rheumatoid arthritis, chondrocytes Arthritis Res Ther 9 5 2007 220
5. T. Aigner, S. Soeder, and J. Haag IL-1b and BMPs - intereactive players of cartilage matrix degradation and regeneration Eur Cells Mater 12 2006 49 56 (Pubitemid 44898115)
6. F. Dell'Accio, C. De Bari, and F.P. Luyten Molecular markers predictive of the capacity of expanded human articular chondrocytes to form stable cartilage in vivo Arthritis Rheum 44 2001 1608 1619 (Pubitemid 32642223)
7. F. Finger, C. Schorle, A. Zien, P. Gebhard, M.B. Goldring, and T. Aigner Molecular phenotyping of human chondrocyte cell lines T/C-28a2, T/C-28a4, and C-28/I2 Arthritis Rheum 48 12 2003 3395 3403 (Pubitemid 37531906)
8. M.B. Goldring, J.R. Birkhead, L-F. Suen, R. Yamin, S. Mizuno, and J. Glowacki Interleukin-1-modualted gene expression in immortalized human chondrocytes J Clin Invest 94 1994 2307 2316
9. S.P. Scully, K.R. Berend, A. Toth, W.N. Qi, Z. Qi, and J.A. Block Interstitial collagenase gene expression correlates with in vivo invasion in human chondrossarcoma Clin Orthop Relat Res 376 2000 291 303 (Pubitemid 30445707)
10. C. De Bari, F. Dell'Accio, and F.P. Luyten Human periosteum-derived cells maintain phenotypic stability and chondrogenic potential throughout expansion regardless of donor age Arthritis Rheum 41 1 2001 85 95 (Pubitemid 32113122)
11. R. Kokenyesi, L. Tan, J. Robbins, and M.B. Goldring Proteoglycan production by immortalized chondrocyte cell lines cultured under conditions that promote expression of the differentiated phenotype Arch Biochem Biophys 383 2000 79 90
12. K.H. Chua, B.S. Aminuddin, N.H. Fuzina, and B.H.I. Ruszymah Insulin-transferrin-selenium prevent human chondrocyte dedifferentiation and promote the formation of high quality tissue engineered human hyaline cartilage Eur Cells Mater 9 2005 58 67 (Pubitemid 41008179)
13. G. Nalesso, J. Sherwood, J. Bertrand, T. Pap, M. Ramachandran, and C. De Bari WNT-3A modulates articular chondrocyte phenotype by activating both canonical and noncanonical pathways J Cell Biol 193 3 2011 551 564
14. H.J. Mankin, H. Dorfman, L. Lippiello, A. Zarins, and Biochemical metabolic abnormalities in articular cartilage from osteo-arthritic human hips II. Correlation of morphology with biochemical and metabolic data J Bone Joint Surg Am 53 1971 523 537
15. F. Dell'Accio, C. De Bari, N.M. Eltawil, P. Vanhummelen, and C. Pitzalis Identification of the molecular response of articular cartilage to injury, by microarray screening: Wnt-16 expression and signaling after injury and in osteoarthritis Arthritis Rheum8 58 5 2008 1410 1421 (Pubitemid 351705930)
16. C. De Bari, F. Dell'Accio, P. Tylzanowski, and F.P. Luyten Multipotent mesenchymal stem cells from adult human synovial membrane Arthritis Rheum 44 8 2001 1928 1942 (Pubitemid 32758241)
17. M.W. Pfaffl A new mathematical model for relative quantification in real-time RT-PCR Nucleic Acids Res 29 2001 e45
18. M. Gosset, F. Berenbaum, S. Thirion, and C. Jacques Primary culture and phenotyping of murine chondrocytes Nat Protoc 3 8 2008 1253 1312
19. M.B. Goldring Culture of immortalized chondrocytes and their use as models of chondrocyte function Methods Mol Med 100 2004 37 52
20. Y.C. Fong, W.H. Yang, S.F. Hsu, H.C. Hsu, K.F. Tseng, and C.J. Hsu 2-methoxiestradiol induces apoptosis and cell cycle arrest in human chondrossarcoma cells J Orthop Res 25 2007 1106 1114
21. T.H. Lai, Y.C. Fong, W.M. Fu, R.S. Yang, and C.H. Tang Stromal cell-derived factor-1 increase avb3 integrin expression and invasion in human chondrossarcoma cells J Cell Physiol 218 2 2008 334 342
22. C. Bui, M. Ouzzine, I. Talhaoui, S. Sharp, K. Prydz, and M.W.H. Coughtrie Epigenetics: methylation-associated repression of heparan sulfate 3-O-sulfotransferase gene expression contributes to the invasive phenotype of H-EMC-SS chondrosarcoma cells FASEB J 24 2010 436 450
23. V. Lefebvre, and P. Smits Transcriptional control of chondrocyte fate and differentiation Birth Defects Res C Embryo Today 75 2005 200 212 (Pubitemid 41456322)
24. M.B. Goldring, M. Otero, D.A. Plumb, C. Dragomir, M. Favero, and K. El Hachem Roles of inflammatory and anabolic cytokines in cartilage metabolism: signals and multiple effectors converge upon MMP-13 regulation in Osteoarthritis Eur Cells Mater 21 2011 202 220
25. J. Flannelly, M.G. Chambers, J. Dudhia, R.M. Hembry, G. Murphy, and R.M. Mason Metalloproteinase and tissue inhibitor of metalloproteinase expression in the murine STR/ort model of osteoarthritis Osteoarthritis Cartilage 10 9 2002 722 733 (Pubitemid 34993960)
26. S.S. Glasson, R. Askew, B. Sheppard, B. Carito, T. Blanchet, and H.L. Ma Deletion of active ADAMTS5 prevents cartilage degradation in a murine model of osteoarthritis Nature 434 2005 644 648 (Pubitemid 40488556)
27. A. Plaas, B. Osborn, Y. Yoshihara, Y. Bai, T. Bloom, and F. Nelson Aggreganolysis in human osteoarthritis: confocal localization and biochemical characterization of ADAMTS5-hyaluronan complexes in articular cartilages Osteoarthritis Cartilage 15 2007 719 734 (Pubitemid 46888154)
28. T. Dehne, R. Schenk, C. Perka, L. Morawietz, A. Pruss, and M. Sittinger Gene expression profiling of primary human articular chondrocytes in high-density micromasses revels patterns of recovery, maintenance, re- and dedifferentiation Gene 462 1-2 2010 8 17
29. K. Andreas, H. Haupl, C. Lubke, J. Ringe, L. Morawietz, and A. Wachtel Antirheumatic drug response signatures in human chondrocytes: potential molecular targets to stimulate cartilage regeneration Arthritis Res Ther 11 1 2009 1 14
30. M.M. Fedewa, T.R. Oegema Jr., M.H. Schwartz, A. MacLeod, and J.L. Lewis Chondrocytes in culture produce a mechanically functional tissue J Orthop Res 16 1998 227 236 (Pubitemid 28249161)
31. B. Johnstone, T.M. Hering, A.I. Caplan, V.M. Goldberg, and J.U. Yoo In vitro chondrogenesis of bone marrowderived mesenchymal progenitor cells Exp Cell Res 238 1998 265 272 (Pubitemid 28366150)
32. Y. Kato, M. Iwamoto, T. Koike, F. Suzuki, and Y. Takano Terminal differentiation and calcification in rabbit chondrocyte cultures grown in centrifuge tubes: regulation by transforming growth factor beta and serum factors Proc Natl Acad Sci USA 85 1998 9552 9556
33. B. de Crombrugghe, V. Lefebvre, R.R. Behringer, W. Bi, S. Murakami, and W. Huang Transcriptional mechanisms of chondrocyte differentiation Matrix Biol 19 5 2000 389 394
34. L. Rosenberg Chemical basis for the histological use of safranin O in the study of articular cartilage J Bone Joint Surg 53A 1971 69 82
35. G. Schulze-Tanzil, P. Souza, V.C.T. John, H.J. Merker, A. Scheid, and M. Shakibaei Redifferentiation of dedifferentiated human chondrocytes in high-density cultures Cell Tissue Res 308 2002 371 379 (Pubitemid 34666963)
36. J. Bertrand, C. Cromme, D. Umlauf, S. Frank, and T. Pap Molecular mechanisms of cartilage remodeling in osteoarthritis Int J Biochem Cell Biol 42 2010 1594 1601
37. T. Kobayashi, K. Notoya, T. Naito, S. Unno, A. Nakamura, and J. Martel-Pelletier A peroxisome proliferator-activated receptor gamma agonist, reduces the progression of experimental osteoarthritis in guinea pigs Arthritis Rheum 52 2 2005 479 487 (Pubitemid 40216312)
38. A. Scharstuhl, E.L. Vitters, P.M. van der Kraan, and W.B. van den Berg Reduction of osteophyte formation and synovial thickening by adenoviral overexpression of transforming growth factor β/bone morphogenetic protein inhibitors during experimental osteoarthritis Arthritis Rheum 48 12 2003 3442 3451 (Pubitemid 37531910)
39. H.L. Glansbeek, H.M. van Beuningen, E.L. Vitters, P.M. van der Kraan, and W.B. van den Berg Stimulation of articular cartilage repair in established arthritis by local administration of transforming growth factor-beta into murine knee joints Lab Invest 78 2 1998 133 142 (Pubitemid 28113452)
40. H. Madry, and S.B. Trippel Efficient lipid-mediated gene transfer to articular chondrocytes Gene Ther 7 2000 286 291 (Pubitemid 30119731)
41. A. Valentin-Opran, J. Wozney, C. Csimma, L. Lilly, and G.E. Riedel Clinical evaluation of recombinant human bone morphogenetic protein-2 Clin Orthop Relat Res 395 2002 110 120 (Pubitemid 34136661)
42. N. Fukui, Y. Zhu, and W.J. Maloney Stimulation of BMP-2 expression by pro-inflammatory cytokines IL-1 and TNF-< in normal and osteoarthritic chondrocytes J Bone Joint Surg Am 85-A Suppl. 3 2003 59 66 (Pubitemid 36951118)
43. D. Chen, M. Zhao, and G.R. Mundy Bone morphogenetic proteins Growth Factors 22 4 2004 233 241 (Pubitemid 40136452)
44. S. Claus, E. Aubert-Foucher, M. Demoor, B. Camuzeaux, A. Paumier, and M. Piperno Chronic exposure of bone morphogenetic protein-2 favors chondrogenic expression in human articular chondrocytes amplified in monolayer cultures J Cell Biochem 111 2010 1642 1651
45. M.B. Goldring, and S.R. Goldring Osteoarthritis J Cell Physiol 213 2007 626 634 (Pubitemid 350058254)
46. V. Krenn, L. Morawietz, G.R. Burmester, R.W. Kinne, U. Mueller-Ladner, and B. Muller Synovitis score: discrimination between chronic low-grade and high-grade synovitis Histopathology 9 2006 358 364 (Pubitemid 44401542)
47. M. Bolla, S. Viappiani, M. Dave, J. Patel, S. Abramson, and M. Attur Effects of NSAIDs and the cyclooxygenase-inhibiting nitric oxide donator (CINOD) NCX 429 on human chondrocytes and cartilage from OA patients [abstract] Arthritis Rheum 62 10 2010 1477
48. T. Sadowski, and J. Steinmeyer Effects of non-steroidal antiinflammatory drugs and dexamethasone on the activity and expression of matrix metalloproteinase-1, matrix metalloproteinase-3 and tissue inhibitor of metalloproteinases-1 by bovine articular chondrocytes Osteoarthritis Cartilage 9 5 2001 407 415 (Pubitemid 32734343)
49. M.J. Shield Anti-inflammatory drugs and their effects on cartilage synthesis and renal function Eur J Rheumatol Inflamm 13 1 1993 7 16
50. E.F. Morand Effects of glucocorticoids on inflammation and arthritis Curr Opin Rheumatol 19 3 2007 302 307 (Pubitemid 46597471)
51. T.D. Warner, and J.A. Mitchell COX-2 selectivity alone does not define the cardiovascular risks associated with non-steroidal anti-inflammatory drugs Lancet 371 2008 270 273
52. S.C. Mastbergen, N.W. Jansen, J.W. Bijlsma, and F.P. Lafeber Differential direct effects of cyclo-oxygenase-1/2 inhibition on proteoglycan turnover of human osteoarthritic cartilage: an in vitro study Arthritis Res Ther 8 1 2006 R2
53. C. Bassleer, Y. Herontin, and P. Franchimont Effects of sodium naproxen on differentiated human chondrocytes cultivated in clusters Clin Rheumatol 11 1 1992 60 65
54. C. Ding Do NSAIDs affect the progression of osteoarthritis? Inflammation 26 3 2002 139 142 (Pubitemid 34596973)
55. Y. Henrotin, and J.Y. Reginster In-vitro differences among nonsteroidal anti-inflammatory drugs in their activities related to osteoarthritis pathophysiology Osteoarthritis Cartilage 7 1999 355 357 (Pubitemid 29244128)
56. P.A. Glazer, M.P. Rosenwasser, and A. Ratcliffe The effect of naproxen and interleukin-1 on proteoglycan catabolism and on neutral metalloproteinase activity in normal articular cartilage in vitro J Clin Pharmacol 33 2 1993 109 114 (Pubitemid 23089631)
57. X. Li, M. Ellman, P. Muddasani, J.H.C. Wang, G. Cs-Szabo, and A.J. van Wijnen Prostaglandin E2 and its cognate EP receptors control human adult articular cartilage homeostasis and are linked to the pathophysiology of osteoarthritis Arthritis Rheum 60 2 2009 513 523
58. M. Miwa, R. Saura, S. Hirata, Y. Hayashi, K. Mizuno, and H. Itoh Induction of apoptosis in bovine articular chondrocyte by prostaglandin E(2) through cAMP-dependent pathway Osteoarthritis Cartilage 8 2000 17 24 (Pubitemid 30032723)
59. M. Attur, H.E. Al-Mussawir, J. Patel, A. Kitay, M. Dave, and G. Palmer Prostaglandin E2 exerts catabolic effects in osteoarthritis cartilage: evidence for signaling via the EP4 receptor J Immunol 181 7 2008 5082 5088
60. E.V. Tchetina, J.A. Di Battista, D.J. Zukor, J. Antoniou, A.R. Poole, and Prostaglandin PGE2 at very low concentrations suppresses collagen cleavage in cultured human osteoarthritic articular cartilage: this involves a decrease in expression of proinflammatory genes, collagenases and COL10A1, a gene linked to chondrocyte hypertrophy Arthritis Res Ther 9 2007 R75
61. S. Otsuka, T. Aoyama, M. Furu, K. Ito, Y. Jin, and A. Nasu PGE2 signal via EP2 receptors evoked by a selective agonist enhances regeneration if injured articular cartilage Osteoarthritis Cartilage 17 2009 529 538
62. M.B. Goldring, K. Fukuo, and J.R. Birkhead Transcriptional suppression by interleukin-1 and interferon-γ of type II collagen gene expression in human chondrocytes J Cell Biochem 54 1994 85 99 (Pubitemid 24073736)
63. M.B. Goldring, L.F. Suen, R. Yamin, and W.F. Lai Regulation of collagen gene expression by prostaglandins and interleukin-1-β in cultured chondrocytes and fibroblasts Am J Ther 3 1996 9 16
64. M. Miyamoto, H. Ito, S. Mukai, T. Kobayashi, H. Yamamoto, and M. Kobayashi Simultaneous stimulation of EP2 and EP4 is essential to the effect of prostaglandin E2 in chondrocyte differentiation Osteoarthritis Cartilage 11 2003 644 652 (Pubitemid 37045683)
65. S.H. Chen, H. Fahmi, Q. Shi, and M. Benderdour Regulation of microsomal prostaglandin E2 synthase-1 and 5-lipoxygenase-activating protein/5-lipoxygenase by 4-hydroxynonenal in human osteoarthritic chondrocytes Arthritis Res Ther 12 1 2010 R21
66. J.T. Dingle The effects of NSAID on the matrix of human articular cartilages Z Rheumatol 58 1999 125 129 (Pubitemid 29315443)
67. A.P.S. Hungin, and D.W.F. Kean Nonsteroidal anti-inflammatory drugs: overused or underused in osteoarthritis? Am J Med 110 1 2001 8 11
68. A.R. Clark Anti-inflammatory functions of glucocorticoid- induced genes Mol Cell Endocrinol 275 2007 79 97 (Pubitemid 47259278)
69. M. Perretti, D'Acquisto, and Annexin A1 and glucocorticoids as effectors of the resolution of inflammation Nat Rev Immunol 9 2009 62 70