Increasing the thermostability of sucrose phosphorylase by a combination of sequence- and structure-based mutagenesis

Protein Engineering, Design and Selection

Volumn 24, Issue 11, 2011, Pages 829-834

  Cerdobbel, An ^a   De Winter, Karel ^a   Aerts, Dirk ^a   Kuipers, Remko ^b,c   Joosten, Henk Jan ^d   Soetaert, Wim ^a   Desmet, Tom ^a  

^a GHENT UNIVERSITY   (Belgium)

^b RADBOUD UNIVERSITY MEDICAL CENTER   (Netherlands)

^c WAGENINGEN UNIVERSITY   (Netherlands)

^d Bio Prodict   (Netherlands)

Author keywords

enzyme engineering; solvent stability; sucrose phosphorylase; thermostability

Indexed keywords

ACCEPTOR MOLECULES; BIFIDOBACTERIUM ADOLESCENTIS; COSOLVENTS; ENZYME ENGINEERING; LOW TEMPERATURES; SOLVENT STABILITY; STRUCTURE-BASED; THERMOSTABILITY; WILD-TYPE ENZYMES;

AMINO ACIDS; ENZYME ACTIVITY; INDUSTRIAL APPLICATIONS; MUTAGENESIS; PHOSPHORYLATION; SUGAR (SUCROSE);

CRYSTAL STRUCTURE;

BACTERIAL ENZYME; ENZYME VARIANT; ORGANIC SOLVENT; PHOSPHORYLASE; SUCROSE PHOSPHORYLASE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; BIFIDOBACTERIUM ADOLESCENTIS; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME ACTIVITY; ENZYME ENGINEERING; ENZYME KINETICS; ENZYME METABOLISM; ENZYME STABILITY; ENZYME STRUCTURE; HYDROPHOBICITY; LOW TEMPERATURE; MUTATION; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; SOLVENT EFFECT; STATIC ELECTRICITY; THERMODYNAMICS; WILD TYPE;

AMINO ACID SEQUENCE; BIFIDOBACTERIUM; ENZYME STABILITY; GLUCOSYLTRANSFERASES; HALF-LIFE; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS; SOLVENTS; STATIC ELECTRICITY; THERMODYNAMICS;

BIFIDOBACTERIUM ADOLESCENTIS;

EID: 80054902432     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzr042     Document Type: Article

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